Microbial selenite(Se(Ⅳ)) reduction to elemental selenium nanoparticles(SeNPs) and chromate(Cr(Ⅵ)) remediation constitute effective detoxification processes. The strain Bacillus wiedmannii ES2-45 can efficiently red...Microbial selenite(Se(Ⅳ)) reduction to elemental selenium nanoparticles(SeNPs) and chromate(Cr(Ⅵ)) remediation constitute effective detoxification processes. The strain Bacillus wiedmannii ES2-45 can efficiently reduce 5 mmol/L Se(Ⅳ) into SeNPs over 14 h and 1 mmol/L Cr(Ⅵ) within 36 h. Proteomic analysis and qRT-PCR revealed that reductases, including dithiol oxidoreductase(DsbA), Fe-S biosynthesis domain-containing protein(Fes),and aldose-6-phosphate reductase(Aldo), and bacillithiol(BSH) were involved in Se(Ⅳ) reduction. Heterologous expression further confirmed the Se(Ⅳ)-reducing function of three genes and enhanced the Se(Ⅳ) and Cr(Ⅵ) resistance ability. Moreover, the difference between Se(Ⅳ) treatments and controls both intra-and extra-cellular BSH concentration per biomass indicated that BSH contributes to Se(Ⅳ) reduction. Selenium-fortified rice was obtained by foliar spray of SeNPs synthesized by the strain ES2-45. To the best of our knowledge, DsbA and Aldo reductase were firstly verified for the role in Se(Ⅳ) reduction and Cr(Ⅵ)resistance. Importantly, it is a promising strategy that B. wiedmannii ES2-45 is served as an efficient bacterium for SeNP synthesis, selenium biofortification and heavy metal bioremediation.展开更多
Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin Rive...Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China： six nitrogen-phosphorus-potassium fertilizer levels were provided（0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply）, with or without inoculation with Glomus mosseae. The volume and concentrations of particle P（PP） and dissolved P（DP） were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields.展开更多
Carbamazepine is frequently detected in waters and hardly eliminated during conventional wastewater treatment processes due to its complicated chemical structure and resistance to biodegradation. A carbamazepine-degra...Carbamazepine is frequently detected in waters and hardly eliminated during conventional wastewater treatment processes due to its complicated chemical structure and resistance to biodegradation. A carbamazepine-degrading bacterium named CBZ-4 was isolated at a low temperature （10℃） from activated sludge in a municipal wastewater treatment plant. Strain CBZ-4, which can use carbamazepine as its sole source of carbon and energy, was identified as Pseudomonas sp. by the 16S rRNA gene sequence. The composition and percentage of fatty acids, which can reveal the cold-adaptation mechanism of strain CBZ-4, were determined. Strain CBZ-4 can effectively degrade carbamazepine at optimal conditions： pH 7.0, 10℃, 150 r/min rotation speed, and 13% inoculation volume. The average removal rate of carbamazepine was 46.6% after 144 hr of incubation. The biodegradation kinetics of carbamazepine by CBZ-4 was fitted via the Monod model. Vmax and Ks were found to be 0.0094 hr^-1 and 32.5 mg/L, respectively.展开更多
Developing an eco-friendly approach for metallic nanoparticles synthesis is important in current nanotechnology research. In this study, green synthesis of gold nanoparticles （AuNPs） was carried out by a newly isola...Developing an eco-friendly approach for metallic nanoparticles synthesis is important in current nanotechnology research. In this study, green synthesis of gold nanoparticles （AuNPs） was carried out by a newly isolated strain Trichoderrna sp. WL-Go. UV-vis spectra of AuNPs showed a surface plasmon resonance peak at 550 nm, and transmission electron microscopy images revealed that the AuNPs were of varied shape with well dispersibility. The optimal conditions for AuNPs synthesis were HAuC14 1.0 mmol/L, biomass 0.5 g and pH 7-11. Moreover, the bio-AuNPs could efficiently catalyze the decolorization of various azo dyes. This research provided a new microbial resource candidate for green synthesis of AuNPs and demonstrated the potential application ofbio-AuNPs for azo dye decolorization.展开更多
Bioflocculant-producing bacteria Agrobacterium tumefaciens F2 and Bacillus sphaeicus F6 were immobilized onto mycelial pellets to investigate the bioflocculant-producing potential of this combined organism and the cor...Bioflocculant-producing bacteria Agrobacterium tumefaciens F2 and Bacillus sphaeicus F6 were immobilized onto mycelial pellets to investigate the bioflocculant-producing potential of this combined organism and the corresponding flocculating efficiency. The atomic force microscope ( AFM) images of mycelial surface indicate that the mycelia pellet can immobilize bioflocculant-producing bacteria F2 and F6 as a biomass carrier. The flocculating efficiency of bioflocculant produced by this combined organism was studied under the optimum flocculating conditions obtained by Response Surface Methodology ( RSM ) . The fermentation yield of the combined mycelial pellet is about 2. 6 g / L,which is higher than that of the free bacteria ( only 2. 2 g / L) . Flocculating efficiency of the combined mycelial pellet was comparable with that of bioflocculant generated by the free bacteria. The bioflocculant yield is enhanced and the flocculating efficiency of the co-culture is uninfluenced after immobilized with mycelial pellet as a carrier. In conclusion,the mycelial pellet is feasible as a biomass carrier for the immobilization of bioflocculant-producing bacteria.展开更多
Flexible aqueous zinc-air batteries with high energy density and safety have garnered significant attention.Gel polymer electrolytes have emerged as the preferred option over conventional liquid electrolytes due to th...Flexible aqueous zinc-air batteries with high energy density and safety have garnered significant attention.Gel polymer electrolytes have emerged as the preferred option over conventional liquid electrolytes due to their ability to prevent electrolyte leakage.In this study,a composite PANa-PVP-TiO_(2)(NH_(2))hydrogel with high alkaline resistance and ionic conductivity is designed,where the inorganic TiO_(2)(NH_(2))nanoparticles are evenly distributed and integrated into the organic dual network of polyacrylate sodium and polyvinyl pyrrolidone.The organic-inorganic hybrid structure enhances the absorption and retention capabilities for electrolyte solution,leading to impressive ionic conductivity of the gel polymer electrolyte throughout the operation of flexible aqueous zinc-air batteries.Additionally,the incorporation of TiO_(2)(NH_(2))nanoparticles and the dual network construction effectively strengthen the mechanical strength and flexibility of the gel polymer electrolyte,suppressing by-products and zinc dendrite formation.The enhancements lead to the extended cycling longevity of zinc symmetric batteries and excellent power density,as well as the prolonged cycle life of flexible aqueous zinc-air batteries.展开更多
Objective: To explore application of targeted contrast enhanced ultrasonography in diagnosis of early stage vascular endothelial injury and diabetic nephropathy. Methods: Targeted Sono VueTM microbubble was prepared b...Objective: To explore application of targeted contrast enhanced ultrasonography in diagnosis of early stage vascular endothelial injury and diabetic nephropathy. Methods: Targeted Sono VueTM microbubble was prepared by attaching anti-TM monoclonal antibody to the surface of ordinary micro-bubble Sono Vue by biotin-avidin bridge method and ultrasonic instrument was used to evaluate the developing situation of targeted microbubble in vitro. Twenty 12-week-old male GK rats and 20 Wistar rats were enrolled in this study, and were randomly divided into targeted angiography group and ordinary angiography group. Targeted microbubbles Sono VueTM or general microbubble Sono Vue were rapidly injected to the rats via tail vein; the developing situation of the two contrast agents in rats kidneys was dynamically observed. Time intensity curve was used to analyse rat kidney perfusion characteristics in different groups. Results: Targeted ultrasound microbubble Sono Vue-TM was successfully constructed, and it could be used to develop an external image. Targeted microbubbles Sono Vue-TM enabled clear development of experimental rat kidney. Time intensity curve shapes of rat kidney of the two groups showed as single apex with steep ascending and slowly descending branch. Compared with the control group, the rising slope of the GK rat renal cortex, medulla in targeted angiography group increased(P<0.05); the peak intensity of medulla increased(P<0.05), and the total area under the curve of medulla increased(P<0.05). Compared with control group, the ascending branch of the GK rat in renal cortex, medulla in ordinary angiography group increased(P<0.05). The peak intensity of the curve increased(P<0.05), and the total area under the curve increased(P<0.05). Compared with the ordinary angiography group, the peak of GK rat medullacurve in targeted angiography group intensity increased(P<0.05), and the total area under the curve increased(P<0.05). Conclusions: Targeted microbubbles Sono VueTM can make a clear development of experimental rat kidney, its stable performance meet the requirement of ultrasonic observation time limit, and it can reflect early changes of blood perfusion in GK rat kindey.展开更多
目的 旨在探讨基于信息寻求风格的术前教育方式在降低经皮冠状动脉介入治疗患者术前焦虑和抑郁的效果.方法 本研究为单盲随机对照试验.2020年4-9月,从中国西南部昆明市1所三级医院的心内科选取114例经皮冠状动脉介入治疗患者,并随机分...目的 旨在探讨基于信息寻求风格的术前教育方式在降低经皮冠状动脉介入治疗患者术前焦虑和抑郁的效果.方法 本研究为单盲随机对照试验.2020年4-9月,从中国西南部昆明市1所三级医院的心内科选取114例经皮冠状动脉介入治疗患者,并随机分配到干预组(n=57)和对照组(n=57).所有患者均获得口头的术前健康教育和纸质版的术前健康教育手册,并在干预前采用中文版米勒应对风格量表监测维度子量表(the Chinese Version of the Monitoring Subscale of the Miller Behavioral Style Scale,C-MMBSS)将患者分为"监测型"和"非敏感型".干预组实施基于信息寻求风格的术前健康教育,对照组实施常规术前健康教育.采用状态-特质焦虑量表(State-Trait Anxiety Inventory,STAI)和抑郁自评量表(Self-rating Depression Scale,SDS)在患者入院时和术前1 h时测量其焦虑和抑郁水平,并在出院时测量术前健康教育满意度和住院时间.结果 共有104例患者完成研究(干预组和对照组各52例).基于信息寻求风格的术前健康教育方式在缓解患者术前焦虑(21.5±2.1比23.1±3.1)、抑郁(21.9±2.1比23.1±2.5)和提高术前健康教育满意度(94.4±6.8比85.2±9.8)方面的效果优于常规术前健康教育(P<0.01).干预组和对照组患者住院时长比较[(8.2±3.2)d比(7.8±3.3)d],差异无统计学意义(P>0.05).对照组患者在常规术前健康教育后术前焦虑水平稍有提高(22.6±3.2比23.1±3.1,P<0.05).结论 研究结果显示,基于信息寻求风格的术前健康教育方式可以降低患者术前焦虑、抑郁水平,提高术前健康教育满意度.展开更多
Stocking and structural composition of a deciduous broad-leaved forest were determined to predict coarse woody debris quantity by quantifying the empirical relationships between these two attributes.The most ecologica...Stocking and structural composition of a deciduous broad-leaved forest were determined to predict coarse woody debris quantity by quantifying the empirical relationships between these two attributes.The most ecologically significant families by stem density were Salicaceae,Betulaceae,Fagaceae,and Aceraceae.P opulus davidiana was the most dominant species followed by B etula dahurica,Quercus mongolica,and Acer mono.The four species accounted for 69.5%of total stems.Numerous small-diameter species characterized the coarse woody debris showing a reversed J-shaped distribution.The coarse debris of P.davidiana,B.dahurica,and Q.mongolica mainly comprised the 10–20 cm size class,whereas A.mono debris was mainly in the 5–10 cm size class.The spatial patterns of different size classes of coarse woody debris were analyzed using the g-function to determine the size of the tree at its death.The results indicate that the spatial patterns at the 0–50 m scale shifted gradually from an aggregated to a random pattern.For some species,the larger coarse debris might change from an aggregated to a random distribution more easily.Given the importance of coarse woody debris in forest ecosystems,its composition and patterns can improve understanding of community structure and dynamics.The aggregation pattern might be due to density dependence and self-thinning effects,as well as by succession and mortality.The four dominant species across the different size classes showed distinct aggregated distribution features at different spatial scales.This suggests a correlation between the dominant species population,size class,and aggregated distribution of coarse woody debris.展开更多
Nitrogen（N） runoff from paddy fields serves as one of the main sources of water pollution. Our aim was to reduce N runoff from paddy fields by fertilizer management and inoculation with arbuscular mycorrhizal fungi...Nitrogen（N） runoff from paddy fields serves as one of the main sources of water pollution. Our aim was to reduce N runoff from paddy fields by fertilizer management and inoculation with arbuscular mycorrhizal fungi（AMF）. In northeast China, Shuangcheng city in Heilongjiang province, a field experiment was conducted, using rice provided with 0%, 20%, 40%, 60%, 80%,and 100% of the local norm of fertilization（including N, phosphorus and potassium）, with or without inoculation with Glomus mosseae. The volume, concentrations of total N（TN）,dissolved N（DN） and particulate N（PN） of runoff water were measured. We found that the local norm of fertilization led to 18.9 kg/ha of N runoff during rice growing season, with DN accounting for 60%–70%. We also found that reduction in fertilization by 20% cut down TN runoff by 8.2% while AMF inoculation decreased N runoff at each fertilizer level and this effect was inhibited by high fertilization. The combination of inoculation with AMF and 80% of the local norm of fertilization was observed to reduce N runoff by 27.2%. Conclusively, we suggested that the contribution of AMF inoculation combined with decreasing fertilization should get more attention to slow down water eutrophication by reducing N runoff from paddy fields.展开更多
Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly...Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model（R^2= 0.9821） being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions（30.71°C, pH 7.14, 4.23%（V/V） inoculum size and 157.1 mg/L initial atrazine concentration）.Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.展开更多
In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was u...In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was used in this research,to quantitatively estimate the NPS load and analyze the temporal and spatial distributions of NPS pollution in Ashi River Basin. The results indicated that SWAT was suitable to simulate stream-flow and water quality in Ashi River Basin. Total Nitrogen which was contributed by NPS( NPS-TN) accounted for 32. 47%-62. 61%,and Total Phosphorus which was contributed by NPS( NPS-TP)accounted for 22. 30%- 57. 85% of the total load respectively. In inter-annual timescale,both NPS-TN and NPS-TP were influenced by stream-flow and fertilizer. However,when compared with fertilizer,NPS pollution was more directly affected by stream-flow. In annual timescale,NPS-TN and NPS-TP mainly occurred in flood season( from May to September). In the aspect of space,spatial differences of NPS-TN and NPS-TP were extremely significant. The spatial variations of NPS pollution were mainly influenced by land use,precipitation,soil and slope.展开更多
A compound bioflocculant CBF,produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture...A compound bioflocculant CBF,produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture medium constituents including carbon source,nitrogen source and C / N ratio,metal ions and ionic strength on CBF production were studied. Flocculating properties of CBF were examined by a series of experiments and CBF had good flocculating activities in kaolin suspension with divalent cations and stable over wide range of p H. Studies of the flocculating properties revealed that the flocculation could be stimulated by cations Ca2+,Mg2+,Fe2+,Al3+and Fe3+. In addition,it was stable at 4-30 ℃ in the presence of Ca Cl2. It was found to be effective for flocculation of a kaolin suspension under neutral and weak alkaline conditions( p H 7. 0- 9. 0),and flocculating activities of higher than 95% were obtained when the CBF concentrations among 6- 14 mg / L at p H 8. 0. The results of this study indicate that CBF is a potential replacement of conventional synthetic flocculants and is widely applied in water treatment and downstream processing of food and fermentation industries.展开更多
To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was ...To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS),polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques.It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were 420～560mg/L and 64～100mg/L,respectively,the corresponding average effluent concentra-tions were 160mg/L and 55mg/L,which were 1.6 and 2.2times higher than those of the national standards in China,respectively,demonstrating the inefficient performances of A/O process.Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption,but not by biodegradation.PCR-DGGE profile analysis suggested that the biological system was species-rich,but there was apparent succession of the bacterial community structure in different locations of the A/O system.Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies.Thus,A/O process was inapplicable for the treatment of complicated petrochemical wastewater,and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.展开更多
Nitrate is considered to be one of the most widely present pollutants leading to eutrophication of environment. The purpose of this work was to isolate and identify new anaerobic denitrifying bacteria from reservoir s...Nitrate is considered to be one of the most widely present pollutants leading to eutrophication of environment. The purpose of this work was to isolate and identify new anaerobic denitrifying bacteria from reservoir sediments and utilize different electron donors for isolates to improve nitrate removal efficiency. Using traditional enrichment approach,one purified anaerobic bacterium( Y12) capable of NO-3-N removal from sediments was obtained. The species identity of Y12 was determined via 16 S rRNA gene sequence analysis to be Acinetobacter. In this work,the fastest denitrification rates were observed with ferrous iron as electron donor.And,slightly slower rates were observed with hydrogen and sodium sulfide as electron donors. However,when used hydrogen gas, ferrous iron and sodium sulfide as electron donors, C / N ratios had little effect on autotrophic denitrification rate at the initial C / N ratio from 1.5 to 9.0. Meanwhile,when made use of hydrogen gas,ferrous iron and sodium sulfide as electron donors,a maximum nitrate removal ratio of 100.00%,91.43%and 87.99% at the temperature of 30 ℃,respectively. Moreover,maximum denitrification activity was observed at p H 6.0-7.0.展开更多
Background and Objectives:Sarcopenia is the progressive loss of muscle mass and strength that can adversely affect an individual’s health and quality of life.The objective of this study was to evaluate the effectiven...Background and Objectives:Sarcopenia is the progressive loss of muscle mass and strength that can adversely affect an individual’s health and quality of life.The objective of this study was to evaluate the effectiveness of a combined nutritional and exercise intervention among older adults who were at risk of sarcopenia.Methods and Study Design:46 older adults were included in a 30±3 days intervention that combined oral nutritional supple ments with resistance exercise.Parameters were measured at baseline(day 0)and after intervention(day 30±3),including routine parameters of sarcopenia,blood tests,and body measurements.The ITT analysis method was used,and the data were analysed using paired t-tests/paired Wilcoxon test,and ANOVA.Results:Among the 46 participants,there were no significant changes in hip circumference(HC),muscle mass of both lower limbs,ap pendicular skeletal muscle mass index(ASMI),and hemoglobin(Hb)after intervention.However,both hand grip strength(GS)significantly increased,as did muscle mass of both upper limbs and the total muscle strength.Blood tests showed a slight increase in albumin(ALB)levels and a significant increase in 25-OH-D levels,while the waist(WC)and calf circumferences(CC)also increased significantly after intervention.Somatic motor per formance improved significantly in the 6-meter walk and 5 sit-ups tests.Conclusions:The combined nutritional and exercise intervention was feasible and effective in improving muscle mass and strength,especially in the up per limbs,as well as somatic motor performance among older adults at risk of sarcopenia.It could be beneficial among three stages of sarcopenia.展开更多
Nanomaterials are increasingly used for biomedical applications; thus, it is important to understand their biological effects. Previous studies suggested that magnetic iron oxide nanoparticles （IONPs） have tissue-re...Nanomaterials are increasingly used for biomedical applications; thus, it is important to understand their biological effects. Previous studies suggested that magnetic iron oxide nanoparticles （IONPs） have tissue-repairing effects. In the present study, we explored cellular effects of IONPs in mesenchymal stem cells （MSCs） and identified the underlying molecular mechanisms. The results showed that our as-prepared IONPs were structurally stable in MSCs and promoted osteogenic differentiation of MSCs as whole particles. Moreover, at the molecular level, we compared the gene expression of MSCs with or without IONP exposure and showed that IONPs upregulated long noncoding RNA INZEB2, which is indispensable for maintaining osteogenesis by MSCs. Furthermore, overexpression of INZEB2 downregulated ZEB2, a factor necessary to repress BMP/Smad- dependent osteogenic transcription. We also demonstrated that the essential role of INZEB2 in osteogenic differentiation was ZEB2-dependent. In summary, we elucidated the molecular basis of IONPs＇ effects on MSCs; these findings may serve as a meaningful theoretical foundation for applications of stem cells to regenerative medicine.展开更多
基金supported by the National Natural Science Foundation of China(No.32370130).
文摘Microbial selenite(Se(Ⅳ)) reduction to elemental selenium nanoparticles(SeNPs) and chromate(Cr(Ⅵ)) remediation constitute effective detoxification processes. The strain Bacillus wiedmannii ES2-45 can efficiently reduce 5 mmol/L Se(Ⅳ) into SeNPs over 14 h and 1 mmol/L Cr(Ⅵ) within 36 h. Proteomic analysis and qRT-PCR revealed that reductases, including dithiol oxidoreductase(DsbA), Fe-S biosynthesis domain-containing protein(Fes),and aldose-6-phosphate reductase(Aldo), and bacillithiol(BSH) were involved in Se(Ⅳ) reduction. Heterologous expression further confirmed the Se(Ⅳ)-reducing function of three genes and enhanced the Se(Ⅳ) and Cr(Ⅵ) resistance ability. Moreover, the difference between Se(Ⅳ) treatments and controls both intra-and extra-cellular BSH concentration per biomass indicated that BSH contributes to Se(Ⅳ) reduction. Selenium-fortified rice was obtained by foliar spray of SeNPs synthesized by the strain ES2-45. To the best of our knowledge, DsbA and Aldo reductase were firstly verified for the role in Se(Ⅳ) reduction and Cr(Ⅵ)resistance. Importantly, it is a promising strategy that B. wiedmannii ES2-45 is served as an efficient bacterium for SeNP synthesis, selenium biofortification and heavy metal bioremediation.
基金supported by National Natural Science Foundation of China (51179041)Major Science and Technology Program for Water Pollution Control and Treatment (No. 2013ZX07201003)the State Key Lab of Urban Water Resource and Environment (Harbin Institute of Technology) (No. 2014TS05)
文摘Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China： six nitrogen-phosphorus-potassium fertilizer levels were provided（0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply）, with or without inoculation with Glomus mosseae. The volume and concentrations of particle P（PP） and dissolved P（DP） were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields.
基金supported by the National Creative Research Group from the National Natural Science Foundation of China (No. 51121062)the National Natural Science Foundation of China (No. 51108120, 51178139)+1 种基金the 4th Special Financial Grant from the China Postdoctoral Science Foundation (No. 201104430)the 46th China Postdoctoral Science Foundation (No. 20090460901)
文摘Carbamazepine is frequently detected in waters and hardly eliminated during conventional wastewater treatment processes due to its complicated chemical structure and resistance to biodegradation. A carbamazepine-degrading bacterium named CBZ-4 was isolated at a low temperature （10℃） from activated sludge in a municipal wastewater treatment plant. Strain CBZ-4, which can use carbamazepine as its sole source of carbon and energy, was identified as Pseudomonas sp. by the 16S rRNA gene sequence. The composition and percentage of fatty acids, which can reveal the cold-adaptation mechanism of strain CBZ-4, were determined. Strain CBZ-4 can effectively degrade carbamazepine at optimal conditions： pH 7.0, 10℃, 150 r/min rotation speed, and 13% inoculation volume. The average removal rate of carbamazepine was 46.6% after 144 hr of incubation. The biodegradation kinetics of carbamazepine by CBZ-4 was fitted via the Monod model. Vmax and Ks were found to be 0.0094 hr^-1 and 32.5 mg/L, respectively.
基金supported by the National Natural Science Foundation of China (Nos. 21176040 and 51508068)the Program for New Century Excellent Talents in University (No. NCET-13-0077)+1 种基金the Fundamental Research Funds for the Central Universities (No. DUT14YQ107)the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. ESK201529)
文摘Developing an eco-friendly approach for metallic nanoparticles synthesis is important in current nanotechnology research. In this study, green synthesis of gold nanoparticles （AuNPs） was carried out by a newly isolated strain Trichoderrna sp. WL-Go. UV-vis spectra of AuNPs showed a surface plasmon resonance peak at 550 nm, and transmission electron microscopy images revealed that the AuNPs were of varied shape with well dispersibility. The optimal conditions for AuNPs synthesis were HAuC14 1.0 mmol/L, biomass 0.5 g and pH 7-11. Moreover, the bio-AuNPs could efficiently catalyze the decolorization of various azo dyes. This research provided a new microbial resource candidate for green synthesis of AuNPs and demonstrated the potential application ofbio-AuNPs for azo dye decolorization.
基金Sponsored by the National Hi-Tech Research and Development Program (Grant No. 2009AA062906)the National Creative Research Group from the National Natural Science Foundation of China (Grant No. 51121062)+1 种基金the State Key Lab of Urban Water Resource and Environment,Harbin Institute of Technology (Grant No. 2010DX09,2010TX03)the Science and Technology Development Program of Jilin Province (Grant No. 201101108)
文摘Bioflocculant-producing bacteria Agrobacterium tumefaciens F2 and Bacillus sphaeicus F6 were immobilized onto mycelial pellets to investigate the bioflocculant-producing potential of this combined organism and the corresponding flocculating efficiency. The atomic force microscope ( AFM) images of mycelial surface indicate that the mycelia pellet can immobilize bioflocculant-producing bacteria F2 and F6 as a biomass carrier. The flocculating efficiency of bioflocculant produced by this combined organism was studied under the optimum flocculating conditions obtained by Response Surface Methodology ( RSM ) . The fermentation yield of the combined mycelial pellet is about 2. 6 g / L,which is higher than that of the free bacteria ( only 2. 2 g / L) . Flocculating efficiency of the combined mycelial pellet was comparable with that of bioflocculant generated by the free bacteria. The bioflocculant yield is enhanced and the flocculating efficiency of the co-culture is uninfluenced after immobilized with mycelial pellet as a carrier. In conclusion,the mycelial pellet is feasible as a biomass carrier for the immobilization of bioflocculant-producing bacteria.
基金supported by the National Natural Science Foundation of China(Grant Nos.51872147,22136003)the Project of Hubei Provincial Department of Education(Grant No.D20221202)+1 种基金the 111 Project(Grant No.D20015)the Hubei Provincial Natural Science Foundation of China(Grant Nos.2022CFA065,2022CFB820).
文摘Flexible aqueous zinc-air batteries with high energy density and safety have garnered significant attention.Gel polymer electrolytes have emerged as the preferred option over conventional liquid electrolytes due to their ability to prevent electrolyte leakage.In this study,a composite PANa-PVP-TiO_(2)(NH_(2))hydrogel with high alkaline resistance and ionic conductivity is designed,where the inorganic TiO_(2)(NH_(2))nanoparticles are evenly distributed and integrated into the organic dual network of polyacrylate sodium and polyvinyl pyrrolidone.The organic-inorganic hybrid structure enhances the absorption and retention capabilities for electrolyte solution,leading to impressive ionic conductivity of the gel polymer electrolyte throughout the operation of flexible aqueous zinc-air batteries.Additionally,the incorporation of TiO_(2)(NH_(2))nanoparticles and the dual network construction effectively strengthen the mechanical strength and flexibility of the gel polymer electrolyte,suppressing by-products and zinc dendrite formation.The enhancements lead to the extended cycling longevity of zinc symmetric batteries and excellent power density,as well as the prolonged cycle life of flexible aqueous zinc-air batteries.
基金supported by Shanghai Municipal Commission of Health and Family Health Planning Key Projects(NO.20134023)
文摘Objective: To explore application of targeted contrast enhanced ultrasonography in diagnosis of early stage vascular endothelial injury and diabetic nephropathy. Methods: Targeted Sono VueTM microbubble was prepared by attaching anti-TM monoclonal antibody to the surface of ordinary micro-bubble Sono Vue by biotin-avidin bridge method and ultrasonic instrument was used to evaluate the developing situation of targeted microbubble in vitro. Twenty 12-week-old male GK rats and 20 Wistar rats were enrolled in this study, and were randomly divided into targeted angiography group and ordinary angiography group. Targeted microbubbles Sono VueTM or general microbubble Sono Vue were rapidly injected to the rats via tail vein; the developing situation of the two contrast agents in rats kidneys was dynamically observed. Time intensity curve was used to analyse rat kidney perfusion characteristics in different groups. Results: Targeted ultrasound microbubble Sono Vue-TM was successfully constructed, and it could be used to develop an external image. Targeted microbubbles Sono Vue-TM enabled clear development of experimental rat kidney. Time intensity curve shapes of rat kidney of the two groups showed as single apex with steep ascending and slowly descending branch. Compared with the control group, the rising slope of the GK rat renal cortex, medulla in targeted angiography group increased(P<0.05); the peak intensity of medulla increased(P<0.05), and the total area under the curve of medulla increased(P<0.05). Compared with control group, the ascending branch of the GK rat in renal cortex, medulla in ordinary angiography group increased(P<0.05). The peak intensity of the curve increased(P<0.05), and the total area under the curve increased(P<0.05). Compared with the ordinary angiography group, the peak of GK rat medullacurve in targeted angiography group intensity increased(P<0.05), and the total area under the curve increased(P<0.05). Conclusions: Targeted microbubbles Sono VueTM can make a clear development of experimental rat kidney, its stable performance meet the requirement of ultrasonic observation time limit, and it can reflect early changes of blood perfusion in GK rat kindey.
文摘目的 旨在探讨基于信息寻求风格的术前教育方式在降低经皮冠状动脉介入治疗患者术前焦虑和抑郁的效果.方法 本研究为单盲随机对照试验.2020年4-9月,从中国西南部昆明市1所三级医院的心内科选取114例经皮冠状动脉介入治疗患者,并随机分配到干预组(n=57)和对照组(n=57).所有患者均获得口头的术前健康教育和纸质版的术前健康教育手册,并在干预前采用中文版米勒应对风格量表监测维度子量表(the Chinese Version of the Monitoring Subscale of the Miller Behavioral Style Scale,C-MMBSS)将患者分为"监测型"和"非敏感型".干预组实施基于信息寻求风格的术前健康教育,对照组实施常规术前健康教育.采用状态-特质焦虑量表(State-Trait Anxiety Inventory,STAI)和抑郁自评量表(Self-rating Depression Scale,SDS)在患者入院时和术前1 h时测量其焦虑和抑郁水平,并在出院时测量术前健康教育满意度和住院时间.结果 共有104例患者完成研究(干预组和对照组各52例).基于信息寻求风格的术前健康教育方式在缓解患者术前焦虑(21.5±2.1比23.1±3.1)、抑郁(21.9±2.1比23.1±2.5)和提高术前健康教育满意度(94.4±6.8比85.2±9.8)方面的效果优于常规术前健康教育(P<0.01).干预组和对照组患者住院时长比较[(8.2±3.2)d比(7.8±3.3)d],差异无统计学意义(P>0.05).对照组患者在常规术前健康教育后术前焦虑水平稍有提高(22.6±3.2比23.1±3.1,P<0.05).结论 研究结果显示,基于信息寻求风格的术前健康教育方式可以降低患者术前焦虑、抑郁水平,提高术前健康教育满意度.
基金supported by The National Science Foundation of China(31770567,31570630)。
文摘Stocking and structural composition of a deciduous broad-leaved forest were determined to predict coarse woody debris quantity by quantifying the empirical relationships between these two attributes.The most ecologically significant families by stem density were Salicaceae,Betulaceae,Fagaceae,and Aceraceae.P opulus davidiana was the most dominant species followed by B etula dahurica,Quercus mongolica,and Acer mono.The four species accounted for 69.5%of total stems.Numerous small-diameter species characterized the coarse woody debris showing a reversed J-shaped distribution.The coarse debris of P.davidiana,B.dahurica,and Q.mongolica mainly comprised the 10–20 cm size class,whereas A.mono debris was mainly in the 5–10 cm size class.The spatial patterns of different size classes of coarse woody debris were analyzed using the g-function to determine the size of the tree at its death.The results indicate that the spatial patterns at the 0–50 m scale shifted gradually from an aggregated to a random pattern.For some species,the larger coarse debris might change from an aggregated to a random distribution more easily.Given the importance of coarse woody debris in forest ecosystems,its composition and patterns can improve understanding of community structure and dynamics.The aggregation pattern might be due to density dependence and self-thinning effects,as well as by succession and mortality.The four dominant species across the different size classes showed distinct aggregated distribution features at different spatial scales.This suggests a correlation between the dominant species population,size class,and aggregated distribution of coarse woody debris.
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment (No. 2012ZX07201003)the National Natural Science Foundation of China (No. 31570505)+1 种基金the Natural Science Foundation of Heilongjiang Province,China (No. E201206)the State Key Lab of Urban Water Resource and Environment (Harbin Institute of Technology) (No. 2014TS05)
文摘Nitrogen（N） runoff from paddy fields serves as one of the main sources of water pollution. Our aim was to reduce N runoff from paddy fields by fertilizer management and inoculation with arbuscular mycorrhizal fungi（AMF）. In northeast China, Shuangcheng city in Heilongjiang province, a field experiment was conducted, using rice provided with 0%, 20%, 40%, 60%, 80%,and 100% of the local norm of fertilization（including N, phosphorus and potassium）, with or without inoculation with Glomus mosseae. The volume, concentrations of total N（TN）,dissolved N（DN） and particulate N（PN） of runoff water were measured. We found that the local norm of fertilization led to 18.9 kg/ha of N runoff during rice growing season, with DN accounting for 60%–70%. We also found that reduction in fertilization by 20% cut down TN runoff by 8.2% while AMF inoculation decreased N runoff at each fertilizer level and this effect was inhibited by high fertilization. The combination of inoculation with AMF and 80% of the local norm of fertilization was observed to reduce N runoff by 27.2%. Conclusively, we suggested that the contribution of AMF inoculation combined with decreasing fertilization should get more attention to slow down water eutrophication by reducing N runoff from paddy fields.
基金supported by the Major Science and Technology Program for Water Pollution Control and Treatment(No.2012ZX07212001)the National Natural Science Foundation of China(No.31570505)the State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(No.2014TS05)
文摘Atrazine, a widely used herbicide, is increasing the agricultural production effectively, while also causing great environmental concern. Efficient atrazine-degrading bacterium is necessary to removal atrazine rapidly to keep a safe environment. In the present study, a new atrazine-degrading strain ZXY-1, identified as Pseudomonas, was isolated. This new isolated strain has a strong ability to biodegrade atrazine with a high efficiency of 9.09 mg/L/hr.Temperature, p H, inoculum size and initial atrazine concentration were examined to further optimize the degradation of atrazine, and the synthetic effect of these factors were investigated by the response surface methodology. With a high quadratic polynomial mathematical model（R^2= 0.9821） being obtained, the highest biodegradation efficiency of 19.03 mg/L/hr was reached compared to previous reports under the optimal conditions（30.71°C, pH 7.14, 4.23%（V/V） inoculum size and 157.1 mg/L initial atrazine concentration）.Overall, this study provided an efficient bacterium and approach that could be potentially useful for the bioremediation of wastewater containing atrazine.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51179041)the Major Science and Technology Program for Water Pollution Control and Treatment(Grant No.2013ZX07201007)+2 种基金Natural Science Foundation of Heilongjiang Province,China(Grant No.E201206)Special Fund for Science and Technology Innovation of Harbin(Grant No.2012RFLXS026)the State Key Lab of Urban Water Resource and Environment(Harbin Institute of Technology)(Grant No.2014TS05)
文摘In order to get a thorough understanding of non-point source pollution,it is essential to examine its temporal and spatial distribution. A physically-based distributed model,Soil and Water Assessment Tool( SWAT),was used in this research,to quantitatively estimate the NPS load and analyze the temporal and spatial distributions of NPS pollution in Ashi River Basin. The results indicated that SWAT was suitable to simulate stream-flow and water quality in Ashi River Basin. Total Nitrogen which was contributed by NPS( NPS-TN) accounted for 32. 47%-62. 61%,and Total Phosphorus which was contributed by NPS( NPS-TP)accounted for 22. 30%- 57. 85% of the total load respectively. In inter-annual timescale,both NPS-TN and NPS-TP were influenced by stream-flow and fertilizer. However,when compared with fertilizer,NPS pollution was more directly affected by stream-flow. In annual timescale,NPS-TN and NPS-TP mainly occurred in flood season( from May to September). In the aspect of space,spatial differences of NPS-TN and NPS-TP were extremely significant. The spatial variations of NPS pollution were mainly influenced by land use,precipitation,soil and slope.
基金Sponsored by the National High Technology Research and Development Program of China(Grant No.2009AA062906)the"Twelfth Five-Year Plan"National Key Technology R&D Program of China(Grant No.2012BAD14B06-04)+2 种基金the National Natural Science Foundation of China(Grant No.51478140&51408200)State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology)(Grant No.2015DX06)Promising Youngsters Training Program of Heilongjiang University of Science and Technology(Grant No.Q20120201)
文摘A compound bioflocculant CBF,produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture medium constituents including carbon source,nitrogen source and C / N ratio,metal ions and ionic strength on CBF production were studied. Flocculating properties of CBF were examined by a series of experiments and CBF had good flocculating activities in kaolin suspension with divalent cations and stable over wide range of p H. Studies of the flocculating properties revealed that the flocculation could be stimulated by cations Ca2+,Mg2+,Fe2+,Al3+and Fe3+. In addition,it was stable at 4-30 ℃ in the presence of Ca Cl2. It was found to be effective for flocculation of a kaolin suspension under neutral and weak alkaline conditions( p H 7. 0- 9. 0),and flocculating activities of higher than 95% were obtained when the CBF concentrations among 6- 14 mg / L at p H 8. 0. The results of this study indicate that CBF is a potential replacement of conventional synthetic flocculants and is widely applied in water treatment and downstream processing of food and fermentation industries.
基金Project supported by the National Basic Research Program(973)of China(No.2004CB418505)the Science and Technology Devel-opment Program of Heilongjiang Province(No.CC05S301),China
文摘To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment,the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS),polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques.It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were 420～560mg/L and 64～100mg/L,respectively,the corresponding average effluent concentra-tions were 160mg/L and 55mg/L,which were 1.6 and 2.2times higher than those of the national standards in China,respectively,demonstrating the inefficient performances of A/O process.Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption,but not by biodegradation.PCR-DGGE profile analysis suggested that the biological system was species-rich,but there was apparent succession of the bacterial community structure in different locations of the A/O system.Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies.Thus,A/O process was inapplicable for the treatment of complicated petrochemical wastewater,and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.
基金Sponsored by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China(Grant No.2012BAC04B02)Open Project of State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(Grant No.QA201518)the Key Laboratory of the Education Department of Shaanxi Province(Grant No.12JS051)
文摘Nitrate is considered to be one of the most widely present pollutants leading to eutrophication of environment. The purpose of this work was to isolate and identify new anaerobic denitrifying bacteria from reservoir sediments and utilize different electron donors for isolates to improve nitrate removal efficiency. Using traditional enrichment approach,one purified anaerobic bacterium( Y12) capable of NO-3-N removal from sediments was obtained. The species identity of Y12 was determined via 16 S rRNA gene sequence analysis to be Acinetobacter. In this work,the fastest denitrification rates were observed with ferrous iron as electron donor.And,slightly slower rates were observed with hydrogen and sodium sulfide as electron donors. However,when used hydrogen gas, ferrous iron and sodium sulfide as electron donors, C / N ratios had little effect on autotrophic denitrification rate at the initial C / N ratio from 1.5 to 9.0. Meanwhile,when made use of hydrogen gas,ferrous iron and sodium sulfide as electron donors,a maximum nitrate removal ratio of 100.00%,91.43%and 87.99% at the temperature of 30 ℃,respectively. Moreover,maximum denitrification activity was observed at p H 6.0-7.0.
基金supported by China National Key R&D Pro gram during the 13th Five-year Plan Period(No.2020YFC2005605)China National Key R&D Program during the 13th Five-year Plan Period(No.2020YFC2005604)the Comprehensive intervention technology and product develop ment for sarcopenia in the elderly National Project of Multidis ciplinary Management of Major Diseases,2020.(No.2199000764).
文摘Background and Objectives:Sarcopenia is the progressive loss of muscle mass and strength that can adversely affect an individual’s health and quality of life.The objective of this study was to evaluate the effectiveness of a combined nutritional and exercise intervention among older adults who were at risk of sarcopenia.Methods and Study Design:46 older adults were included in a 30±3 days intervention that combined oral nutritional supple ments with resistance exercise.Parameters were measured at baseline(day 0)and after intervention(day 30±3),including routine parameters of sarcopenia,blood tests,and body measurements.The ITT analysis method was used,and the data were analysed using paired t-tests/paired Wilcoxon test,and ANOVA.Results:Among the 46 participants,there were no significant changes in hip circumference(HC),muscle mass of both lower limbs,ap pendicular skeletal muscle mass index(ASMI),and hemoglobin(Hb)after intervention.However,both hand grip strength(GS)significantly increased,as did muscle mass of both upper limbs and the total muscle strength.Blood tests showed a slight increase in albumin(ALB)levels and a significant increase in 25-OH-D levels,while the waist(WC)and calf circumferences(CC)also increased significantly after intervention.Somatic motor per formance improved significantly in the 6-meter walk and 5 sit-ups tests.Conclusions:The combined nutritional and exercise intervention was feasible and effective in improving muscle mass and strength,especially in the up per limbs,as well as somatic motor performance among older adults at risk of sarcopenia.It could be beneficial among three stages of sarcopenia.
文摘Nanomaterials are increasingly used for biomedical applications; thus, it is important to understand their biological effects. Previous studies suggested that magnetic iron oxide nanoparticles （IONPs） have tissue-repairing effects. In the present study, we explored cellular effects of IONPs in mesenchymal stem cells （MSCs） and identified the underlying molecular mechanisms. The results showed that our as-prepared IONPs were structurally stable in MSCs and promoted osteogenic differentiation of MSCs as whole particles. Moreover, at the molecular level, we compared the gene expression of MSCs with or without IONP exposure and showed that IONPs upregulated long noncoding RNA INZEB2, which is indispensable for maintaining osteogenesis by MSCs. Furthermore, overexpression of INZEB2 downregulated ZEB2, a factor necessary to repress BMP/Smad- dependent osteogenic transcription. We also demonstrated that the essential role of INZEB2 in osteogenic differentiation was ZEB2-dependent. In summary, we elucidated the molecular basis of IONPs＇ effects on MSCs; these findings may serve as a meaningful theoretical foundation for applications of stem cells to regenerative medicine.
