Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration vi...Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.展开更多
Due to the digital transformation tendency among cultural institutions and the substantial influence of the social media platform,the demands of visual communication keep increasing for promoting traditional cultural ...Due to the digital transformation tendency among cultural institutions and the substantial influence of the social media platform,the demands of visual communication keep increasing for promoting traditional cultural artifacts online.As an effective medium,posters serve to attract public attention and facilitate broader engagement with cultural artifacts.However,existing poster generation methods mainly rely on fixed templates and manual design,which limits their scalability and adaptability to the diverse visual and semantic features of the artifacts.Therefore,we propose CAPGen,an automated aesthetic Cultural Artifacts Poster Generation framework built on a Multimodal Large Language Model(MLLM)with integrated iterative optimization.During our research,we collaborated with designers to define principles of graphic design for cultural artifact posters,to guide the MLLM in generating layout parameters.Later,we generated these parameters into posters.Finally,we refined the posters using an MLLM integrated with a multi-round iterative optimization mechanism.Qualitative results show that CAPGen consistently outperforms baseline methods in both visual quality and aesthetic performance.Furthermore,ablation studies indicate that the prompt,iterative optimization mechanism,and design principles significantly enhance the effectiveness of poster generation.展开更多
The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium thres...The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium threshold up to high energies. Both the positronium formation and ionization continuum channels are included in the calculations via a complex equivalent local potential. The positronium formation, ionization, elastic and 2~3S–2~3P excitation, and total scattering cross sections are all presented and compared with the available information.展开更多
基金supported by the National Natural Science Foundation of China,No.81571211(to FL)the Natural Science Foundation of Shanghai,No.22ZR1476800(to CH)。
文摘Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.
基金supported by the National Key Research and Development Program of China(2023YFF0906502)the Postgraduate Research and Innovation Project of Hunan Province under Grant(CX20240473).
文摘Due to the digital transformation tendency among cultural institutions and the substantial influence of the social media platform,the demands of visual communication keep increasing for promoting traditional cultural artifacts online.As an effective medium,posters serve to attract public attention and facilitate broader engagement with cultural artifacts.However,existing poster generation methods mainly rely on fixed templates and manual design,which limits their scalability and adaptability to the diverse visual and semantic features of the artifacts.Therefore,we propose CAPGen,an automated aesthetic Cultural Artifacts Poster Generation framework built on a Multimodal Large Language Model(MLLM)with integrated iterative optimization.During our research,we collaborated with designers to define principles of graphic design for cultural artifact posters,to guide the MLLM in generating layout parameters.Later,we generated these parameters into posters.Finally,we refined the posters using an MLLM integrated with a multi-round iterative optimization mechanism.Qualitative results show that CAPGen consistently outperforms baseline methods in both visual quality and aesthetic performance.Furthermore,ablation studies indicate that the prompt,iterative optimization mechanism,and design principles significantly enhance the effectiveness of poster generation.
基金Project supported by the Natural Science Foundation of Heilongjiang Province of China(Grant No.12541160)
文摘The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium threshold up to high energies. Both the positronium formation and ionization continuum channels are included in the calculations via a complex equivalent local potential. The positronium formation, ionization, elastic and 2~3S–2~3P excitation, and total scattering cross sections are all presented and compared with the available information.
