Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key step...Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.展开更多
背景常规注气结肠镜检查最为困难的部分在左半结肠,插镜过程中患者可有明显不适,有研究报道水交换技术可明显减轻内镜检查过程中患者的疼痛和不适感,然而,左半结肠水交换技术与常规注气比较效果如何,目前与之相关的研究尚少.目的比较左...背景常规注气结肠镜检查最为困难的部分在左半结肠,插镜过程中患者可有明显不适,有研究报道水交换技术可明显减轻内镜检查过程中患者的疼痛和不适感,然而,左半结肠水交换技术与常规注气比较效果如何,目前与之相关的研究尚少.目的比较左半结肠水交换(water exchange,WE)结肠镜与常规注气(air insufflation,AI)结肠镜检查的临床应用效果.方法2020-06/2020-12,至我院消化内镜中心行结肠镜检查的连续患者,符合纳入标准的患者,按照随机数字表以(1:1)进行随机分为左半结肠WE组与常规AI组,记录两组的盲肠插镜成功率,到达盲肠的进镜时间,退镜时间,腺瘤检出率(adenoma detection rate,ADR),疼痛视觉模拟评分(visual analogue scale,VAS),穿孔等检查相关并发症.结果根据纳入与排除标准,共筛选出276例患者,其中左半结肠WE组139例,常规AI组137例,两组盲肠插镜成功率、退镜时间以及腺瘤检出率差异无统计学意义,但左半结肠WE组较常规AI组进镜时间明显缩短(4.6 min vs 5.3 min;P<0.05),VAS评分更低(2.7 min vs 3.3 min;P<0.05),两组间均无穿孔等检查相关并发症发生.结论左半结肠水交换结肠镜较常规注气结肠镜检查明显缩短进镜时间,减轻患者的疼痛和不适,值得临床选择使用.展开更多
基金supported by grants from the Guangzhou Science and Technology Program Key Project(Grant No.201803010044)Guangdong Province College Characteristic Innovation Project(2019KTSCX011)+2 种基金Guangdong Province Natural Sciences Fund Project(2021A1515012480)the Key Areas Research and Development Program of Guangzhou(202103030003)Guangdong Province Special Fund Projects(Yueziranzihe,2021,No.50).
文摘Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same results.In conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.
文摘背景常规注气结肠镜检查最为困难的部分在左半结肠,插镜过程中患者可有明显不适,有研究报道水交换技术可明显减轻内镜检查过程中患者的疼痛和不适感,然而,左半结肠水交换技术与常规注气比较效果如何,目前与之相关的研究尚少.目的比较左半结肠水交换(water exchange,WE)结肠镜与常规注气(air insufflation,AI)结肠镜检查的临床应用效果.方法2020-06/2020-12,至我院消化内镜中心行结肠镜检查的连续患者,符合纳入标准的患者,按照随机数字表以(1:1)进行随机分为左半结肠WE组与常规AI组,记录两组的盲肠插镜成功率,到达盲肠的进镜时间,退镜时间,腺瘤检出率(adenoma detection rate,ADR),疼痛视觉模拟评分(visual analogue scale,VAS),穿孔等检查相关并发症.结果根据纳入与排除标准,共筛选出276例患者,其中左半结肠WE组139例,常规AI组137例,两组盲肠插镜成功率、退镜时间以及腺瘤检出率差异无统计学意义,但左半结肠WE组较常规AI组进镜时间明显缩短(4.6 min vs 5.3 min;P<0.05),VAS评分更低(2.7 min vs 3.3 min;P<0.05),两组间均无穿孔等检查相关并发症发生.结论左半结肠水交换结肠镜较常规注气结肠镜检查明显缩短进镜时间,减轻患者的疼痛和不适,值得临床选择使用.
