Abstract [Objective] This study was conducted to evaluate the ecotoxicity of boscalid to adult zebrafish （Danio rerio）. [Method] The activities of antioxidant enzymes superoxide dismutase （SOD）, catalase （CAT）, ...Abstract [Objective] This study was conducted to evaluate the ecotoxicity of boscalid to adult zebrafish （Danio rerio）. [Method] The activities of antioxidant enzymes superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, glutathione peroxidase （GPx）, as well as a non-enzymatic antioxidant malondialdehyde （MDA）, in the liver were measured 3, 7, 14 and 21 d post exposure （dpe） to 0.02 （1/100 of acute toxicity）, 0.036 （monitored concentration）, 0.08 （1/20 of acute toxicity）, 0.16 （1/10 of acute toxicity） and 0.32 mg/L （1/5 of acute toxicity） boscalid using a semi-static method. [Result] SOD, CAT, POD, GPx and MDA activity in the liver of zebrafish varied with boscalid concentration and exposure time. Boscalid significantly enhanced MDA content at 21 dpe. A significant upregulation of the activity of SOD, CAT, POD and GPx at 7 dpe was observed, suggesting that boscalid resulted in oxidative stress and lipid peroxidation. [Conclusion] These results show that these biomarkers are all appropriate for monitoring oxidative stress and the lipid peroxidation status of fish after exposure to boscalid. Key words Boscalid; Zebrafish; Antioxidant enzyme展开更多
目的:探讨心血管危险因素对非动脉炎性前部缺血性视神经病变(NAION)的发生及视功能的影响。方法:选取2014-06/2018-06于我科就诊的单眼初发NAION患者(NAION组)和与其基本资料匹配的非NAION患者(对照组)各68例68眼,检测两组患者同型半胱...目的:探讨心血管危险因素对非动脉炎性前部缺血性视神经病变(NAION)的发生及视功能的影响。方法:选取2014-06/2018-06于我科就诊的单眼初发NAION患者(NAION组)和与其基本资料匹配的非NAION患者(对照组)各68例68眼,检测两组患者同型半胱氨酸(Hcy)、血脂、叶酸、维生素B12水平,并进行颈动脉多普勒超声检查,NAION组患者同时进行视功能检查。结果:与对照组相比,NAION组患者Hcy(24.8±13.9μmol/L vs 11.1±8.2μmol/L)、血浆中总胆固醇(4.5±1.0mmol/L vs 3.8±0.7mmol/L)、甘油三酯(2.0±0.9mmol/L vs 1.5±0.5mmol/L)、低密度脂蛋白(2.9±0.8mmol/L vs 2.3±0.5mmol/L)水平均升高(P<0.05),维生素B12水平明显下降(315.6±214.5pg/mL vs 467.9±198.2pg/mL,P<0.05),但两组患者颈内动脉阻力指数和内径无差异。NAION组患者患眼视野缺损值为16.6±7.5dB,Hcy、维生素B12、叶酸及血脂水平以及是否存在全身疾病均不是NAION视野损害的危险因素,而图形视觉诱发电位P100波幅和潜伏期峰值均与视野缺损值相关。结论:高同型半胱氨酸、高血脂和低维生素B12水平是NAION发生的危险因素,但与NAION的视野损害程度无关;视觉诱发电位的波幅和潜伏期峰值可一定程度上反映视野损害的程度。展开更多
Objective To evaluate the value of whole-body diffusion weighted imaging （WB-DWI） on detection of malignant metastasis. Methods Forty-six patients with malignant tumors underwent WB-DWI examinations between April 20...Objective To evaluate the value of whole-body diffusion weighted imaging （WB-DWI） on detection of malignant metastasis. Methods Forty-six patients with malignant tumors underwent WB-DWI examinations between April 2007 and August 2007 in our hospital. Before WB-DWI examination, the primary cancers of all the patients were confirmed by pathology, and the TNM-stage was assessed with conventional magnetic resonance imaging （MRI） or computed tomography （CT）. WB-DWI was performed using short TI inversion recovery echo-planar imaging （STIR-EPI） sequence. Abnormal high signal intensities on WB-DWI were considered as metastases. The results of WB-DWI were compared with other imaging modalities. For the assessment of the diagnostic capability of WB-DWI, WB-DWI were compared with CT for demonstrating mediastinal lymph node metastases and lung metastases, and with conventional MRI for demonstrating metastases in other locations. Results WB-DWI demonstrated 143 focuses, 14 routine imaging. The number of bone metastases depicted of which were diagnosed to be benign lesions in on WB-DWI and routine imaging was 85 and 86; lymph node metastases was 17 and 18; liver metastases was 14 and 14; lung metastases was 4 and 8; and brain metastases was 6 and 8, respectively. WB-DWI failed to detect 12 metastatic lesions including 3 osteoplastic bone metastases, 4 lung metastases, 3 mediastinal lymph node metastases, and 2 brain metastases Four metastatic lesions including 2 deltopectoral lymph nodes and 2 rib metastases were detected with WB-DWI alone, all of which evolved greatly during clinical follow-up for more than 6 months. WB-DWI had higher detection rates for metastatic lesions in liver, bone, and lymph nodes than those in lung and brain （ X^2=30, P〈0.001）. Conclusions WB-DWI could detect most of metastatic lesions that were diagnosed with conventional MRI and CT. The limitations of WB-DWI might be had high false-positive rate and low efficiency in detecting mecliastinal lymph node, brain, and lung metastases.展开更多
Lily is a well-known ornamental plant with a diversity of fragrant types.Basic information on lily floral scent compounds has been obtained for only a few accessions,and little is known about Lilium aroma types,the te...Lily is a well-known ornamental plant with a diversity of fragrant types.Basic information on lily floral scent compounds has been obtained for only a few accessions,and little is known about Lilium aroma types,the terpene synthase genes that may play roles in the production of key volatiles,or the range of monoterpenes that these genes produce.In this study,41 cultivars were analyzed for volatile emissions,and a total of 46 individual volatile compounds were identified,16 for the first time in lilies.Lily accessions were classified into six groups according to the composition of major scent components:faint-scented,cool,fruity,musky,fruity-honey,and lily.Monoterpenes were one of the main groups of volatiles identified,and attention was focused on terpene synthase(TPS)genes,which encode enzymes that catalyze the last steps in monoterpene synthesis.Thirty-two candidate monoterpene synthase cDNAs were obtained from 66 lily cultivars,and 64 SNPs were identified.Two InDels were also shown to result from variable splicing,and sequence analysis suggested that different transcripts arose from the same gene.All identified nucleotide substitution sites were highly correlated with the amounts of myrcene emitted,and InDel site 230 was highly correlated with the emission of all major monoterpenoid components,especially(E)-β-ocimene.Heterologous expression of five cDNAs cloned from faint-scented and strong-scented lilies showed that their corresponding enzymes could convert geranyl diphosphate to(E)-β-ocimene,α-pinene,and limonene.The findings from this study provide a major resource for the assessment of lily scent volatiles and will be helpful in breeding of improved volatile components.展开更多
Chinese bayberry （Myrica rubra Sieb. et Zucc.） is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat （SSR） molecular mark...Chinese bayberry （Myrica rubra Sieb. et Zucc.） is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat （SSR） molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content （PIC） varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera （syn. More/la cerifera） and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between ＇Biqi＇ and ＇Dongkui＇. A neighbor-joining （N J） tree was con- structed to assess the genetic relationships among accessions, and the elite accessions ＇Y2010-70＇, ＇Y2012-140＇, and ＇Y2012-145＇, were characterized as potential new genotypes for cultivation.展开更多
Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells,which are involved in autoimmune diseases.However,it is unknown whether attenuated activa...Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells,which are involved in autoimmune diseases.However,it is unknown whether attenuated activated healthy autologous T-cell immunization could increase anti-tumor immune responses.To this end,C57B1/6 mice were immunized with attenuated activated autologous T cells.The splenocytes from immunized mice showed a higher proliferative ability than that from naive mice.The special phenotype analysis showed that there were more CD8+T cells and CD62L+T cells in immunized mice after 24 h of culture with 10%fetal calf serum complete medium in vitro(P〈0.01).These results demonstrated that this immunization may activate T cells in vivo.Furthermore,the splenocytes from immunized mice revealed resistance to activation-induced cell death(AICD)in vitro.To further study the relative genes that are responsible for the higher proliferation and resistance to AICD,the expression of Fas/Fas ligand(FasL)and GADD4513 was measured by real-time PCR.The results indicated that GADD45βtranscription was higher in the splenocytes from immunized mice than that in the naive mice.In addition,the Fas expression showed a parallel higher,but FasL did not change obviously.To investigate the biologic functions induced by immunization in vivo,a tumor model was established by EL-4 tumor cell inoculation in C57/B1 mice.Mice receiving autologous T-cell immunization had significantly inhibited tumor growth in vivo(P〈0.01).This study implicated that immunization with attenuated activated autologous T cells enhances anti-tumor immune responses that participate in tumor growth inhibition.展开更多
As the agricultural internet of things(IoT)technology has evolved,smart agricultural robots needs to have both flexibility and adaptability when moving in complex field environments.In this paper,we propose the concep...As the agricultural internet of things(IoT)technology has evolved,smart agricultural robots needs to have both flexibility and adaptability when moving in complex field environments.In this paper,we propose the concept of a vision-based navigation system for the agricultural IoT and a binocular vision navigation algorithm for smart agricultural robots,which can fuse the edge contour and the height information of rows of crop in images to extract the navigation parameters.First,the speeded-up robust feature(SURF)extracting and matching algorithm is used to obtain featuring point pairs from the green crop row images observed by the binocular parallel vision system.Then the confidence density image is constructed by integrating the enhanced elevation image and the corresponding binarized crop row image,where the edge contour and the height information of crop row are fused to extract the navigation parameters(θ,d)based on the model of a smart agricultural robot.Finally,the five navigation network instruction sets are designed based on the navigation angleθand the lateral distance d,which represent the basic movements for a certain type of smart agricultural robot working in a field.Simulated experimental results in the laboratory show that the algorithm proposed in this study is effective with small turning errors and low standard deviations,and can provide a valuable reference for the further practical application of binocular vision navigation systems in smart agricultural robots in the agricultural IoT system.展开更多
Regulatory T cells （Treg） play important roles in immune system homeostasis, and may also be involved in tumor immunotolerance by suppressing Th1 immune response which is involved in anti-tumor immunity. We have pre...Regulatory T cells （Treg） play important roles in immune system homeostasis, and may also be involved in tumor immunotolerance by suppressing Th1 immune response which is involved in anti-tumor immunity. We have previously reported that immunization with attenuated activated autologous T cells leads to enhanced anti-tumor immunity and upregulated Thl responses in vivo. However, the underlying molecular mechanisms are not well understood. Here we show that Treg function was significantly downregulated in mice that received immunization of attenuated activated autologous T cells. We found that Foxp3 expression decreased in CD4＋CD25＋ T cells from the immunized mice. Moreover, CD4＋CD25＋Foxp3＋ Treg obtained from immunized mice exhibited diminished immunosuppression ability compared to those from naive mice. Further analysis showed that the serum of immunized mice contains a high level ofanti-CD25 antibody （about 30 ng/ml, p〈0.01 vs controls）. Consistent with a role ofanti-CD25 response in the downregulation of Treg, adoptive transfer of serum from immunized mice to naive mice led to a significant decrease in Treg population and function in recipient mice. The triggering of anti-CD25 response in immunized mice can be explained by the fact that CD25 was induced to a high level in the ConA activated autologous T cells used for immunization. Our results demonstrate for the first time that immunization with attenuated activated autologous T cells evokes anti-CD25 antibody production, which leads to impeded CD4＋CD25＋Foxp3＋ Treg expansion and function in vivo. We suggest that dampened Treg function likely contributes to enhanced Thl response in immunized mice and is at least part of the mechanism underlying the boosted anti-tumor immunity.展开更多
Bone marrow mononuclear cells （BMMCs） can be directly harvested from the donor, allowing for easier application compared with bone marrow mesenchymal stem cells. The present study hypothesized that BMMC transplantat...Bone marrow mononuclear cells （BMMCs） can be directly harvested from the donor, allowing for easier application compared with bone marrow mesenchymal stem cells. The present study hypothesized that BMMC transplantation could ameliorate cerebral ischemia in a mouse model. BMMCs were double-labeled with PKH26 and 4＇, 6-diamidino-2-phenylindole （DAPI）, followed by infusion into mice via the tail vein to induce focal cerebral ischemia. At 14 days after transplantation, morphological and neurofunctional recovery were analyzed. PKH26 and DAPI double-positive BMMCs were detected in the cerebral hemisphere of all transplantation mice. Following BMMC administration, there was significant difference in neurofunctional recovery, but no significant difference in survival rates between BMMC-treated mice and other mice. These results demonstrate that transplanted BMMCs migrate to brain tissue and promote neurological function recovery in a mouse model of cerebral ischemia.展开更多
Clinical reports have demonstrated that the Kongsheng Zhenzhong pill （KSZZP）, a classical prescription deriving from Valuable Prescription for Emergencies, has good therapeutic effects on vascular dementia. However,...Clinical reports have demonstrated that the Kongsheng Zhenzhong pill （KSZZP）, a classical prescription deriving from Valuable Prescription for Emergencies, has good therapeutic effects on vascular dementia. However, the mechanisms that mediate its effects remain unclear. In this study, the expression of N-methyI-D-aspartate receptor 1 mRNA, the content of nitric oxide, and the concentration of calcium in neurons was determined with in situ hybridization, spectrophotometry and flow cytometry, respectively. In addition, the expressions of N-methyI-D-aspartate receptor 1, nerve growth factor protein, and glial cell line-derived neurotrophic factor protein were detected with immunohistochemistry. We found that KSZZP could significantly decrease the expression of N-methyI-D-aspartate receptor 1 mRNA and protein, the content of nitric oxide, and the concentration of calcium in neurons. KSZZP also increased the expression of nerve growth factor and glial cell line-derived neurotrophic factor protein in the hippocampus CA1 region and in the cerebral cortex. Morris water maze and passive avoidance tests verified that KSZZP ameliorated the cognitive impairments of vascular dementia rats. Moreover, the KSZZP-induced improvements in the cognitive functions of vascular dementia rats were correlated with both inhibition of N-methyl-D-aspartate-induced excitable neurotoxicity and elevation of neurotrophic factor expression.展开更多
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable c...Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid （HA） on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids ＇Sorbonne＇ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations （_〉2.0 mg/L） within 45 d after transplanting （DAT）. The activities of three major starch synthetic enzymes （including adenosine 5＇-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase） were enhanced dramatically after HA application especially low concentration HA （LHA）, indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg （2.9 times that under the control treatment） and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.展开更多
Purpose:.To present a case of frosted branch angiitis with tuberculous meningoencephalitis who was followed up for 18 months after treatment.Methods: Case report.Results:.A 19-year-old female with tuberculous meningoe...Purpose:.To present a case of frosted branch angiitis with tuberculous meningoencephalitis who was followed up for 18 months after treatment.Methods: Case report.Results:.A 19-year-old female with tuberculous meningoencephalitis complained of bilateral blurred vision, and presented with frosted branch angiitis and macula edema in both eyes. Treatment with systemic glucocorticoid and standard anti-tuberculosis medicine totally resolved the retinal periphlebitis and macular edema, with vision improvement achieved at the 18-month follow-up.Conclusion: Frosted branch angiitis could be secondary to tuberculous meningoencephalitis. Based on standard anti-tuberculosis medicine, systemic glucocorticoid may help in the remission of frosted branch angiitis.展开更多
文摘Abstract [Objective] This study was conducted to evaluate the ecotoxicity of boscalid to adult zebrafish （Danio rerio）. [Method] The activities of antioxidant enzymes superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, glutathione peroxidase （GPx）, as well as a non-enzymatic antioxidant malondialdehyde （MDA）, in the liver were measured 3, 7, 14 and 21 d post exposure （dpe） to 0.02 （1/100 of acute toxicity）, 0.036 （monitored concentration）, 0.08 （1/20 of acute toxicity）, 0.16 （1/10 of acute toxicity） and 0.32 mg/L （1/5 of acute toxicity） boscalid using a semi-static method. [Result] SOD, CAT, POD, GPx and MDA activity in the liver of zebrafish varied with boscalid concentration and exposure time. Boscalid significantly enhanced MDA content at 21 dpe. A significant upregulation of the activity of SOD, CAT, POD and GPx at 7 dpe was observed, suggesting that boscalid resulted in oxidative stress and lipid peroxidation. [Conclusion] These results show that these biomarkers are all appropriate for monitoring oxidative stress and the lipid peroxidation status of fish after exposure to boscalid. Key words Boscalid; Zebrafish; Antioxidant enzyme
文摘目的:探讨心血管危险因素对非动脉炎性前部缺血性视神经病变(NAION)的发生及视功能的影响。方法:选取2014-06/2018-06于我科就诊的单眼初发NAION患者(NAION组)和与其基本资料匹配的非NAION患者(对照组)各68例68眼,检测两组患者同型半胱氨酸(Hcy)、血脂、叶酸、维生素B12水平,并进行颈动脉多普勒超声检查,NAION组患者同时进行视功能检查。结果:与对照组相比,NAION组患者Hcy(24.8±13.9μmol/L vs 11.1±8.2μmol/L)、血浆中总胆固醇(4.5±1.0mmol/L vs 3.8±0.7mmol/L)、甘油三酯(2.0±0.9mmol/L vs 1.5±0.5mmol/L)、低密度脂蛋白(2.9±0.8mmol/L vs 2.3±0.5mmol/L)水平均升高(P<0.05),维生素B12水平明显下降(315.6±214.5pg/mL vs 467.9±198.2pg/mL,P<0.05),但两组患者颈内动脉阻力指数和内径无差异。NAION组患者患眼视野缺损值为16.6±7.5dB,Hcy、维生素B12、叶酸及血脂水平以及是否存在全身疾病均不是NAION视野损害的危险因素,而图形视觉诱发电位P100波幅和潜伏期峰值均与视野缺损值相关。结论:高同型半胱氨酸、高血脂和低维生素B12水平是NAION发生的危险因素,但与NAION的视野损害程度无关;视觉诱发电位的波幅和潜伏期峰值可一定程度上反映视野损害的程度。
文摘Objective To evaluate the value of whole-body diffusion weighted imaging （WB-DWI） on detection of malignant metastasis. Methods Forty-six patients with malignant tumors underwent WB-DWI examinations between April 2007 and August 2007 in our hospital. Before WB-DWI examination, the primary cancers of all the patients were confirmed by pathology, and the TNM-stage was assessed with conventional magnetic resonance imaging （MRI） or computed tomography （CT）. WB-DWI was performed using short TI inversion recovery echo-planar imaging （STIR-EPI） sequence. Abnormal high signal intensities on WB-DWI were considered as metastases. The results of WB-DWI were compared with other imaging modalities. For the assessment of the diagnostic capability of WB-DWI, WB-DWI were compared with CT for demonstrating mediastinal lymph node metastases and lung metastases, and with conventional MRI for demonstrating metastases in other locations. Results WB-DWI demonstrated 143 focuses, 14 routine imaging. The number of bone metastases depicted of which were diagnosed to be benign lesions in on WB-DWI and routine imaging was 85 and 86; lymph node metastases was 17 and 18; liver metastases was 14 and 14; lung metastases was 4 and 8; and brain metastases was 6 and 8, respectively. WB-DWI failed to detect 12 metastatic lesions including 3 osteoplastic bone metastases, 4 lung metastases, 3 mediastinal lymph node metastases, and 2 brain metastases Four metastatic lesions including 2 deltopectoral lymph nodes and 2 rib metastases were detected with WB-DWI alone, all of which evolved greatly during clinical follow-up for more than 6 months. WB-DWI had higher detection rates for metastatic lesions in liver, bone, and lymph nodes than those in lung and brain （ X^2=30, P〈0.001）. Conclusions WB-DWI could detect most of metastatic lesions that were diagnosed with conventional MRI and CT. The limitations of WB-DWI might be had high false-positive rate and low efficiency in detecting mecliastinal lymph node, brain, and lung metastases.
基金sponsored by the Natural Science Foundation of Shanxi Province(201601D011077)Shanxi Key Project in Agriculture(201803D221011-2).
文摘Lily is a well-known ornamental plant with a diversity of fragrant types.Basic information on lily floral scent compounds has been obtained for only a few accessions,and little is known about Lilium aroma types,the terpene synthase genes that may play roles in the production of key volatiles,or the range of monoterpenes that these genes produce.In this study,41 cultivars were analyzed for volatile emissions,and a total of 46 individual volatile compounds were identified,16 for the first time in lilies.Lily accessions were classified into six groups according to the composition of major scent components:faint-scented,cool,fruity,musky,fruity-honey,and lily.Monoterpenes were one of the main groups of volatiles identified,and attention was focused on terpene synthase(TPS)genes,which encode enzymes that catalyze the last steps in monoterpene synthesis.Thirty-two candidate monoterpene synthase cDNAs were obtained from 66 lily cultivars,and 64 SNPs were identified.Two InDels were also shown to result from variable splicing,and sequence analysis suggested that different transcripts arose from the same gene.All identified nucleotide substitution sites were highly correlated with the amounts of myrcene emitted,and InDel site 230 was highly correlated with the emission of all major monoterpenoid components,especially(E)-β-ocimene.Heterologous expression of five cDNAs cloned from faint-scented and strong-scented lilies showed that their corresponding enzymes could convert geranyl diphosphate to(E)-β-ocimene,α-pinene,and limonene.The findings from this study provide a major resource for the assessment of lily scent volatiles and will be helpful in breeding of improved volatile components.
基金supported by the Ningbo Science and Technology Bureau(No.2012C10012)the Science and Technology Project of Zhejiang Province(No.2012C12904)the Yuyao Financial Bureau for Chinese Bayberry Breeding Program,China
文摘Chinese bayberry （Myrica rubra Sieb. et Zucc.） is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat （SSR） molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content （PIC） varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera （syn. More/la cerifera） and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between ＇Biqi＇ and ＇Dongkui＇. A neighbor-joining （N J） tree was con- structed to assess the genetic relationships among accessions, and the elite accessions ＇Y2010-70＇, ＇Y2012-140＇, and ＇Y2012-145＇, were characterized as potential new genotypes for cultivation.
基金supported by Science and Technology Commission of Shanghai Municipality(Nos.03DJ14009,04DZ14902,05ZR14055,054319928)Shanghai Munici-pal Education(No.05BZ26)+1 种基金Shanghai Leading Aca-demic Discipline Project(T0206)Translational Research Seed Fund of Institute of Health Sciences(No.XK2172).
文摘Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells,which are involved in autoimmune diseases.However,it is unknown whether attenuated activated healthy autologous T-cell immunization could increase anti-tumor immune responses.To this end,C57B1/6 mice were immunized with attenuated activated autologous T cells.The splenocytes from immunized mice showed a higher proliferative ability than that from naive mice.The special phenotype analysis showed that there were more CD8+T cells and CD62L+T cells in immunized mice after 24 h of culture with 10%fetal calf serum complete medium in vitro(P〈0.01).These results demonstrated that this immunization may activate T cells in vivo.Furthermore,the splenocytes from immunized mice revealed resistance to activation-induced cell death(AICD)in vitro.To further study the relative genes that are responsible for the higher proliferation and resistance to AICD,the expression of Fas/Fas ligand(FasL)and GADD4513 was measured by real-time PCR.The results indicated that GADD45βtranscription was higher in the splenocytes from immunized mice than that in the naive mice.In addition,the Fas expression showed a parallel higher,but FasL did not change obviously.To investigate the biologic functions induced by immunization in vivo,a tumor model was established by EL-4 tumor cell inoculation in C57/B1 mice.Mice receiving autologous T-cell immunization had significantly inhibited tumor growth in vivo(P〈0.01).This study implicated that immunization with attenuated activated autologous T cells enhances anti-tumor immune responses that participate in tumor growth inhibition.
基金the National Natural Science Foundationof China(No.31760345).
文摘As the agricultural internet of things(IoT)technology has evolved,smart agricultural robots needs to have both flexibility and adaptability when moving in complex field environments.In this paper,we propose the concept of a vision-based navigation system for the agricultural IoT and a binocular vision navigation algorithm for smart agricultural robots,which can fuse the edge contour and the height information of rows of crop in images to extract the navigation parameters.First,the speeded-up robust feature(SURF)extracting and matching algorithm is used to obtain featuring point pairs from the green crop row images observed by the binocular parallel vision system.Then the confidence density image is constructed by integrating the enhanced elevation image and the corresponding binarized crop row image,where the edge contour and the height information of crop row are fused to extract the navigation parameters(θ,d)based on the model of a smart agricultural robot.Finally,the five navigation network instruction sets are designed based on the navigation angleθand the lateral distance d,which represent the basic movements for a certain type of smart agricultural robot working in a field.Simulated experimental results in the laboratory show that the algorithm proposed in this study is effective with small turning errors and low standard deviations,and can provide a valuable reference for the further practical application of binocular vision navigation systems in smart agricultural robots in the agricultural IoT system.
基金This work was supported by National Natural Science Foundation of China(No.30671945)Science and Technology Commission of Shanghai Municipality(Nos.06JC14044,05ZR14055,054319928,04DZ14902)+2 种基金Shanghai Municipal Education(No.05BZ26)Shanghai Leading Academic Discipline Project(T0206)Science Foundation of Shanghai Institute of Immunology(No.07-A04,to Ningli Li).
文摘Regulatory T cells （Treg） play important roles in immune system homeostasis, and may also be involved in tumor immunotolerance by suppressing Th1 immune response which is involved in anti-tumor immunity. We have previously reported that immunization with attenuated activated autologous T cells leads to enhanced anti-tumor immunity and upregulated Thl responses in vivo. However, the underlying molecular mechanisms are not well understood. Here we show that Treg function was significantly downregulated in mice that received immunization of attenuated activated autologous T cells. We found that Foxp3 expression decreased in CD4＋CD25＋ T cells from the immunized mice. Moreover, CD4＋CD25＋Foxp3＋ Treg obtained from immunized mice exhibited diminished immunosuppression ability compared to those from naive mice. Further analysis showed that the serum of immunized mice contains a high level ofanti-CD25 antibody （about 30 ng/ml, p〈0.01 vs controls）. Consistent with a role ofanti-CD25 response in the downregulation of Treg, adoptive transfer of serum from immunized mice to naive mice led to a significant decrease in Treg population and function in recipient mice. The triggering of anti-CD25 response in immunized mice can be explained by the fact that CD25 was induced to a high level in the ConA activated autologous T cells used for immunization. Our results demonstrate for the first time that immunization with attenuated activated autologous T cells evokes anti-CD25 antibody production, which leads to impeded CD4＋CD25＋Foxp3＋ Treg expansion and function in vivo. We suggest that dampened Treg function likely contributes to enhanced Thl response in immunized mice and is at least part of the mechanism underlying the boosted anti-tumor immunity.
文摘Bone marrow mononuclear cells （BMMCs） can be directly harvested from the donor, allowing for easier application compared with bone marrow mesenchymal stem cells. The present study hypothesized that BMMC transplantation could ameliorate cerebral ischemia in a mouse model. BMMCs were double-labeled with PKH26 and 4＇, 6-diamidino-2-phenylindole （DAPI）, followed by infusion into mice via the tail vein to induce focal cerebral ischemia. At 14 days after transplantation, morphological and neurofunctional recovery were analyzed. PKH26 and DAPI double-positive BMMCs were detected in the cerebral hemisphere of all transplantation mice. Following BMMC administration, there was significant difference in neurofunctional recovery, but no significant difference in survival rates between BMMC-treated mice and other mice. These results demonstrate that transplanted BMMCs migrate to brain tissue and promote neurological function recovery in a mouse model of cerebral ischemia.
基金the National Basic Research Program of China(973Program),No.2007CB512601Science and Technology Development Plan of TCM in Shandong Province,No.2009-006Science and Technology Plan in Colleges and Universities of Shandong Province,No.J11LF60,J11LF08
文摘Clinical reports have demonstrated that the Kongsheng Zhenzhong pill （KSZZP）, a classical prescription deriving from Valuable Prescription for Emergencies, has good therapeutic effects on vascular dementia. However, the mechanisms that mediate its effects remain unclear. In this study, the expression of N-methyI-D-aspartate receptor 1 mRNA, the content of nitric oxide, and the concentration of calcium in neurons was determined with in situ hybridization, spectrophotometry and flow cytometry, respectively. In addition, the expressions of N-methyI-D-aspartate receptor 1, nerve growth factor protein, and glial cell line-derived neurotrophic factor protein were detected with immunohistochemistry. We found that KSZZP could significantly decrease the expression of N-methyI-D-aspartate receptor 1 mRNA and protein, the content of nitric oxide, and the concentration of calcium in neurons. KSZZP also increased the expression of nerve growth factor and glial cell line-derived neurotrophic factor protein in the hippocampus CA1 region and in the cerebral cortex. Morris water maze and passive avoidance tests verified that KSZZP ameliorated the cognitive impairments of vascular dementia rats. Moreover, the KSZZP-induced improvements in the cognitive functions of vascular dementia rats were correlated with both inhibition of N-methyl-D-aspartate-induced excitable neurotoxicity and elevation of neurotrophic factor expression.
基金Project supported by the National High-Tech R&D Program(863) of China(No.2011AA100208)the Zhejiang Provincial Natural Science Foundation of China(No.LY12C15003)
文摘Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid （HA） on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids ＇Sorbonne＇ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations （_〉2.0 mg/L） within 45 d after transplanting （DAT）. The activities of three major starch synthetic enzymes （including adenosine 5＇-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase） were enhanced dramatically after HA application especially low concentration HA （LHA）, indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg （2.9 times that under the control treatment） and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.
文摘Purpose:.To present a case of frosted branch angiitis with tuberculous meningoencephalitis who was followed up for 18 months after treatment.Methods: Case report.Results:.A 19-year-old female with tuberculous meningoencephalitis complained of bilateral blurred vision, and presented with frosted branch angiitis and macula edema in both eyes. Treatment with systemic glucocorticoid and standard anti-tuberculosis medicine totally resolved the retinal periphlebitis and macular edema, with vision improvement achieved at the 18-month follow-up.Conclusion: Frosted branch angiitis could be secondary to tuberculous meningoencephalitis. Based on standard anti-tuberculosis medicine, systemic glucocorticoid may help in the remission of frosted branch angiitis.
