生物完整性指数(Index of biotic integrity,IBI)被广泛运用于河流的健康评价.然而,随着人类活动对河流影响的增加,传统指示生物多样性的损失降低了评价的准确性.在高度受影响的水体环境中,微生物群落被证明是更敏感的生态健康指标.本...生物完整性指数(Index of biotic integrity,IBI)被广泛运用于河流的健康评价.然而,随着人类活动对河流影响的增加,传统指示生物多样性的损失降低了评价的准确性.在高度受影响的水体环境中,微生物群落被证明是更敏感的生态健康指标.本研究采用Illumina高通量测序技术,于2018年冬季和2019年春季对无锡市境内8条河流的微生物群落组成进行了分析,从群落组成/相对丰度、丰富度/多样性、敏感性/耐受性3个角度确定了候选指标.并对传统的核心指标筛选方法进行了改进,通过差异性分析、箱线图检验、Speraman相关性分析,建立了冬、春两季的微生物生物完整性指标框架.评价结果表明,M-IBI能有效区分不同程度的受损位点,且与水质状况和物理生境存在显著的相关性.总体而言,M-IBI为城市河流生态状况提供了一种新的评价工具.展开更多
目的应用生物信息学方法探究猪重组NK-lysin(prNK-lysin)抑制肝癌细胞转移潜在的作用靶点和通路。方法将肝癌细胞设置为空白对照组,PBS处理组和prNK-lysin处理组,37℃作用6 h后,应用高效液相色谱串联质谱对肽段进行鉴定,按照Foldchange=...目的应用生物信息学方法探究猪重组NK-lysin(prNK-lysin)抑制肝癌细胞转移潜在的作用靶点和通路。方法将肝癌细胞设置为空白对照组,PBS处理组和prNK-lysin处理组,37℃作用6 h后,应用高效液相色谱串联质谱对肽段进行鉴定,按照Foldchange=1.2倍且P<0.05筛选出差异表达蛋白。基于GO、KEGG等数据库对差异表达蛋白进行GO功能分析和KEGG通路分析。运用RT-qPCR验证细胞中多肽-N-乙酰半乳糖胺基转移酶13(GALNT13)、跨膜蛋白51(TMEM51)和FKBP脯酰异构酶3(FKBP3)的mRNA相对表达量。Western blot验证FKBP3的蛋白表达量。结果蛋白组学表明,与空白对照组相比,prNK-lysin处理组中有1989个差异表达蛋白;与PBS处理组相比,prNK-lysin处理组中有2753个差异表达蛋白;PBS处理组和空白对照组相比,有15个差异表达蛋白。相对于PBS处理组和空白对照组,prNK-lysin处理组中共有1909个差异表达蛋白。GO和KEGG分析表明差异表达蛋白主要参与Viral process、translational initiation、RNAbinding等过程,主要富集于Ribosome、Protein process in endoplasmic reticulum、RNA transport等通路。RT-qPCR表明,与空白对照组相比,prNK-lysin处理组显著升高了细胞内GALNT13(1.54±0.06 vs 1.02±0.17,P<0.05)和TMEM51(1.27±0.07 vs 1.00±0.04,P<0.01)的mRNA相对表达量,显著降低了FKBP3(0.43±0.06 vs 1.02±0.24,P<0.05)的mRNA相对表达量。Western blotting表明prNK-lysin处理组与空白对照组相比显著降低了细胞内FKBP3(0.68±0.02 vs 1.02±0.03,P<0.001)的蛋白表达量。结论prNK-lysin处理后肝癌细胞SMMOL/LC-7721的蛋白质组与空白组相比发生显著变化,prNK-lysin作用于FKBP3蛋白,并能通过影响细胞内氧化磷酸化和糖酵解等通路发挥其抑制作用。展开更多
OBJECTIVE: To evaluate the effect and safety of miniscalpel-needle(MSN) on reducing the pain of myofascial pain syndrome(MPS).METHODS: We reviewed the available literatures inception up to February 2014 using Pubmed, ...OBJECTIVE: To evaluate the effect and safety of miniscalpel-needle(MSN) on reducing the pain of myofascial pain syndrome(MPS).METHODS: We reviewed the available literatures inception up to February 2014 using Pubmed, EMBASE, Cochrane Library, Chinese National Knowledge Infrastructure Database, Chinese Biomedical Database and Wanfang Database.RESULTS: Eight randomized controlled trials were finally identified. The main controls involved acupuncture, medications, injection, massage and cupping. We found that all of the studies agreed on the potential benefit of MSN as a strategy for MPS and the superiority compared to the controls, however,randomized methods applied in most of the trials could be criticized for their high or unclear risk of bias. Further research is also needed to clarify questions around the appropriate frequency and number of treatment sessions of MSN.CONCLUSION: This review shows that MSN might have the effect on MPS, even though there were some limitations in the studies included in the review. Studies with robust methodology are warranted to further test its pain-relieving effect on MPS.展开更多
文摘生物完整性指数(Index of biotic integrity,IBI)被广泛运用于河流的健康评价.然而,随着人类活动对河流影响的增加,传统指示生物多样性的损失降低了评价的准确性.在高度受影响的水体环境中,微生物群落被证明是更敏感的生态健康指标.本研究采用Illumina高通量测序技术,于2018年冬季和2019年春季对无锡市境内8条河流的微生物群落组成进行了分析,从群落组成/相对丰度、丰富度/多样性、敏感性/耐受性3个角度确定了候选指标.并对传统的核心指标筛选方法进行了改进,通过差异性分析、箱线图检验、Speraman相关性分析,建立了冬、春两季的微生物生物完整性指标框架.评价结果表明,M-IBI能有效区分不同程度的受损位点,且与水质状况和物理生境存在显著的相关性.总体而言,M-IBI为城市河流生态状况提供了一种新的评价工具.
文摘目的应用生物信息学方法探究猪重组NK-lysin(prNK-lysin)抑制肝癌细胞转移潜在的作用靶点和通路。方法将肝癌细胞设置为空白对照组,PBS处理组和prNK-lysin处理组,37℃作用6 h后,应用高效液相色谱串联质谱对肽段进行鉴定,按照Foldchange=1.2倍且P<0.05筛选出差异表达蛋白。基于GO、KEGG等数据库对差异表达蛋白进行GO功能分析和KEGG通路分析。运用RT-qPCR验证细胞中多肽-N-乙酰半乳糖胺基转移酶13(GALNT13)、跨膜蛋白51(TMEM51)和FKBP脯酰异构酶3(FKBP3)的mRNA相对表达量。Western blot验证FKBP3的蛋白表达量。结果蛋白组学表明,与空白对照组相比,prNK-lysin处理组中有1989个差异表达蛋白;与PBS处理组相比,prNK-lysin处理组中有2753个差异表达蛋白;PBS处理组和空白对照组相比,有15个差异表达蛋白。相对于PBS处理组和空白对照组,prNK-lysin处理组中共有1909个差异表达蛋白。GO和KEGG分析表明差异表达蛋白主要参与Viral process、translational initiation、RNAbinding等过程,主要富集于Ribosome、Protein process in endoplasmic reticulum、RNA transport等通路。RT-qPCR表明,与空白对照组相比,prNK-lysin处理组显著升高了细胞内GALNT13(1.54±0.06 vs 1.02±0.17,P<0.05)和TMEM51(1.27±0.07 vs 1.00±0.04,P<0.01)的mRNA相对表达量,显著降低了FKBP3(0.43±0.06 vs 1.02±0.24,P<0.05)的mRNA相对表达量。Western blotting表明prNK-lysin处理组与空白对照组相比显著降低了细胞内FKBP3(0.68±0.02 vs 1.02±0.03,P<0.001)的蛋白表达量。结论prNK-lysin处理后肝癌细胞SMMOL/LC-7721的蛋白质组与空白组相比发生显著变化,prNK-lysin作用于FKBP3蛋白,并能通过影响细胞内氧化磷酸化和糖酵解等通路发挥其抑制作用。
基金Supported by Grants from the National Natural Science Foundation of China(Effect of Electroacupuncture at Weizhong(BL 40)Acupoint for Skeletal Muscle Satellite Cells and Inflammatory Reaction during the Repairment Of Rabbit Lumbar Muscle Injury,No.81141120)the Specialized Research Fund for the Doctoral Program of Higher Education of Ministry of Education of China(Effect of Acupuncture on Mechanical Characteristics of Myofascial Pain Syndrome of Rabbits,No.20100013110014)
文摘OBJECTIVE: To evaluate the effect and safety of miniscalpel-needle(MSN) on reducing the pain of myofascial pain syndrome(MPS).METHODS: We reviewed the available literatures inception up to February 2014 using Pubmed, EMBASE, Cochrane Library, Chinese National Knowledge Infrastructure Database, Chinese Biomedical Database and Wanfang Database.RESULTS: Eight randomized controlled trials were finally identified. The main controls involved acupuncture, medications, injection, massage and cupping. We found that all of the studies agreed on the potential benefit of MSN as a strategy for MPS and the superiority compared to the controls, however,randomized methods applied in most of the trials could be criticized for their high or unclear risk of bias. Further research is also needed to clarify questions around the appropriate frequency and number of treatment sessions of MSN.CONCLUSION: This review shows that MSN might have the effect on MPS, even though there were some limitations in the studies included in the review. Studies with robust methodology are warranted to further test its pain-relieving effect on MPS.
