Loss of multiyear ice(MYI)is of great importance for Arctic climate and marine systems and can be monitored using active and passive microwave satellite data.In this paper,we describe an upgraded classification algori...Loss of multiyear ice(MYI)is of great importance for Arctic climate and marine systems and can be monitored using active and passive microwave satellite data.In this paper,we describe an upgraded classification algorithm using the data from the scatterometer and radiometer sensors onboard the Chinese Haiyang-2B(HY-2B)satellite to identify MYI and first-year ice(FYI).The proposed method was established based on K-means and fuzzy clustering(K-means+FC)and was used to focus on the transition zone where the ice condition is complex due to the highly commixing of MYI and FYI,leading to the high challenge for accurate classification of sea ice.The K-means algorithm was applied to preliminarily classify MYI using the combination of scatterometer and radiometer data,followed by applying fuzzy clustering to reclassify MYI in the transition zone.The HY-2B K-means+FC results were compared with the ice type products[including the Ocean and Sea Ice Satellite Application Facility(OSI SAF)sea ice type product and the Equal-Area Scalable Earth-Grid sea ice age dataset],and showed agreement in the time series of MYI extent.Intercomparisons in the transition zone indicated that the HY-2B K-means+FC results can identify more old ice than the OSI SAF product,but with an underestimation in identifying second-year ice.Comparisons between K-means and Kmeans+FC results were performed using regional ice charts and Sentinel-1 synthetic aperture radar(SAR)data.By adding fuzzy clustering,the MYI is more consistent with the ice charts,with the overall accuracy(OA)increasing by 0.9%–6.5%.Comparing against SAR images,it is suggested that more scattered MYI floes can be identified by fuzzy clustering,and the OA is increased by about 3%in middle freezing season and 7%–20%in early and late freezing season.展开更多
Ferroptosis,a type of cell death that mainly involves iron metabolism imbalance and lipid peroxidation,is strongly correlated with the phagocytic response caused by bleeding after spinal cord injury.Thus,in this study...Ferroptosis,a type of cell death that mainly involves iron metabolism imbalance and lipid peroxidation,is strongly correlated with the phagocytic response caused by bleeding after spinal cord injury.Thus,in this study,bulk RNA sequencing data(GSE47681 and GSE5296)and single-cell RNA sequencing data(GSE162610)were acquired from gene expression databases.We then conducted differential analysis and immune infiltration analysis.Atf3 and Piezo1 were identified as key ferroptosis genes through random forest and least absolute shrinkage and selection operator algorithms.Further analysis of single-cell RNA sequencing data revealed a close relationship between ferroptosis and cell types such as macrophages/microglia and their intrinsic state transition processes.Differences in transcription factor regulation and intercellular communication networks were found in ferroptosis-related cells,confirming the high expression of Atf3 and Piezo1 in these cells.Molecular docking analysis confirmed that the proteins encoded by these genes can bind cycloheximide.In a mouse model of T8 spinal cord injury,low-dose cycloheximide treatment was found to improve neurological function,decrease levels of the pro-inflammatory cytokine inducible nitric oxide synthase,and increase levels of the anti-inflammatory cytokine arginase 1.Correspondingly,the expression of the ferroptosis-related gene Gpx4 increased in macrophages/microglia,while the expression of Acsl4 decreased.Our findings reveal the important role of ferroptosis in the treatment of spinal cord injury,identify the key cell types and genes involved in ferroptosis after spinal cord injury,and validate the efficacy of potential drug therapies,pointing to new directions in the treatment of spinal cord injury.展开更多
To investigate the strength degradation characteristics and microscopic damage mechanisms of moraine soil under hydro-thermo-mechanical coupling conditions,a series of X-ray Diffraction(XRD),standard triaxial testing,...To investigate the strength degradation characteristics and microscopic damage mechanisms of moraine soil under hydro-thermo-mechanical coupling conditions,a series of X-ray Diffraction(XRD),standard triaxial testing,Scanning Electron Microscopy(SEM),and Nuclear Magnetic Resonance(NMR)experiments were conducted.The mechanical property degradation laws and evolution characteristics of the microscopic pore structure of moraine soil under Freeze-Thaw(F-T)conditions were revealed.After F-T cycles,the stress-strain curves of moraine soil showed a strain-softening trend.In the early stage of F-T cycles(0–5 cycles),the shear strength and elastic modulus exhibited damage rate of approximately 10.33%±0.8%and 16.60%±1.2%,respectively.In the later stage(10–20 cycles),the strength parameters fluctuated slightly and tended to stabilize.The number of F-T cycles was negatively exponentially correlated with cohesion,while showing only slight fluctuation in the internal friction angle,thereby extending the Mohr-Coulomb strength criterion for moraine soil under F-T cycles.The NMR experiments quantitatively characterized the evolution of the internal pore structure of moraine soil under F-T cycles.As the number of F-T cycles increased,fine and micro pores gradually expanded and merged due to the frost-heaving effect during the water-ice phase transition,forming larger pores.The proportion of large and medium pores increased to 59.55%±2.1%(N=20),while that of fine and micro pores decreased to 40.45%±2.1%(N=20).The evolution of pore structure characteristics was essentially completed in the later stage of F-T cycles(10–20 cycles).This study provides a theoretical foundation and technical support for major engineering construction and disaster prevention in the Qinghai-Xizang Plateau.展开更多
BACKGROUND Due to the dry and cold climate,the obvious temperature difference between day and night,and the low oxygen content of the air in the plateau area,people are prone to upper respiratory tract diseases,and of...BACKGROUND Due to the dry and cold climate,the obvious temperature difference between day and night,and the low oxygen content of the air in the plateau area,people are prone to upper respiratory tract diseases,and often the condition is prolonged,and the patients are prone to anxiety and uneasiness,which may be related to the harshness of the plateau environment,somatic discomfort due to the lack of oxygen,anxiety about the disease,and other factors.AIM To investigate the effects of cognitive behavioral therapy(CBT)on anxiety,sleep disorders,and hypoxia tolerance in patients with high-altitude respiratory diseases.METHODS A total of 2337 patients with high-altitude-related respiratory diseases treated at our hospital between November 2023 and January 2024 were selected as the study subjects.The subjects’pre-high-altitude residential altitude was approximately 1700 meters.They were divided into two groups.Both groups were given symptomatic treatment,and the control group implemented conventional nursing intervention,while the research group simultaneously conducted CBT intervention;assessed the degree of health knowledge of the two groups,and applied the Hamilton Anxiety Scale and the Pittsburgh Sleep Quality Index to assess the anxiety and sleep quality of the patients before and after the intervention,respectively.It also observed the length and efficiency of sleep,and detected the level of serum hypoxia inducible factor-1α,erythropoietin(EPO)and clinical intervention before and after intervention.EPO levels,and investigated satisfaction with the clinical intervention.RESULTS The rate of excellent health knowledge in the intervention group was 93.64%,which was higher than that in the control group(74.23%;P<0.05).Before the intervention,there was no significant difference in Hamilton Anxiety Scale and Pittsburgh Sleep Quality Index scores between the two groups(P>0.05),and after the intervention,the scores of the study group were significantly lower than those of the control group(P<0.05).There was no significant difference in sleep duration and sleep efficiency between the groups before the intervention(P>0.05),and after the intervention,the scores of the study group were significantly larger than those of the control group(P<0.05).There was no significant difference in serum hypoxia inducible factor-1αand EPO between the two groups before intervention(P>0.05),and both research groups were significantly lower than the control group after intervention(P<0.05).According to the questionnaire survey,the intervention satisfaction of the study group was 95.53%,which was higher than that of the control group(80.14%;P<0.05).CONCLUSION The CBT intervention in the treatment of patients with high-altitude-related respiratory diseases helps improve patients'health knowledge,relieve anxiety,improve sleep quality and hypoxia tolerance,and improve nursing satisfaction.展开更多
Objective Arsenic trioxide(ATO)is a clinically effective anticancer agent used in the treatment of leukemia.However,it exerts adverse effects on non-tumor cells,including bone marrow mesenchymal stem cells(BMSCs).This...Objective Arsenic trioxide(ATO)is a clinically effective anticancer agent used in the treatment of leukemia.However,it exerts adverse effects on non-tumor cells,including bone marrow mesenchymal stem cells(BMSCs).This study aims to investigate the protective role and molecular mechanism of traditional Chinese medicine Astragalus polysaccharides(APS)in mitigating ATO-induced apoptosis in BMSCs.Methods BMSCs exposed to ATO(0.5μmol/L)were treated with APS(20,40,100,and 200μg/mL).Cell viability,proliferation,and migration were assessed by using MTT,EdU staining,Transwell,and scratch wound healing assays.Apoptosis was evaluated via TUNEL assay,Hoechst 33258 staining,and flow cytometry.Intracellular reactive oxygen species(ROS)and mitochondrial membrane potential were measured by using DCFH-DA and JC-1 staining.Apoptotic protein expression was analyzed by Western blotting.Results ATO exposure significantly inhibited the proliferation and migration of BMSCs and induced apoptosis,while APS markedly attenuated the apoptosis of BMSCs induced by ATO,and significantly improved cell proliferation and migration(P<0.01).Mechanistically,APS effectively reduced ATO-induced ROS(P<0.01),while the protein expression of Bcl-2-associated X protein(Bax)and cleaved Caspase-3 was significantly decreased(P<0.05),and the protein expression of Bcl-2 was significantly increased(P<0.01).In addition,APS markedly decreased the protein expression of c-Jun N-terminal kinase(Jnk)and p38 in ATO-activated BMSCs(P<0.05),and significantly decreased the protein expression of p16 and p53(P<0.01),and increased the protein expression of phosphorylated protein kinase B(p-Akt)and phosphorylated extracellular signal-regulated kinase(p-Erk)(P<0.01,0.05).Conclusion Our study reveals that APS exert significant protective effects against ATO-induced apoptosis in BMSCs.The mechanisms involve suppressing ROS generation,maintaining mitochondrial membrane stability,enhancing cell viability,migration,and proliferation,as well as inhibiting Jnk and p38 mitogen-activated protein kinase(p38 MAPK)signaling pathways.The findings highlight potential molecular targets and novel strategies for the clinical prevention and treatment of ATO-related toxicity.展开更多
In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the i...In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.展开更多
FAU zeolites have emerged as multifunctional materials with broad applications in catalysis and adsorption,owing to their hierarchical pore architectures,elevated specific surface areas,and adjustable extra-framework ...FAU zeolites have emerged as multifunctional materials with broad applications in catalysis and adsorption,owing to their hierarchical pore architectures,elevated specific surface areas,and adjustable extra-framework cationic sites.This review provides a critical overview of recent advances in FAU zeolite research with emphasis on their roles in environmental pollutant mitigation.A bibliometric analysis was performed to ascertain worldwide research trends,cooperation networks,and principal theme areas.Strategies for synthesis and functionalization,including crystallization pathways,one-pot methods,and post-synthetic modifications,were systematically evaluated for their capacity to tailor structural and physicochemical properties.Environmental applications were discussed in detail,particularly in heavy metal extraction,CO_(2)capture,and catalytic NOx reduction.Despite these advances,challenges persisted,notably restricted chemical stability under extreme pH conditions,scalability obstacles from laboratory to industrial production,and the necessity for enhanced catalytic efficiency.By integrating fundamental understanding with application-oriented perspectives,this review identifies existing knowledge gaps and delineates future directions for the rational design of FAU zeolites toward sustainable environmental remediation.展开更多
Lattice materials have demonstrated promising potential in engineering applications owing to their exceptional lightweight,high specific strength,and tunable mechanical properties.However,the traditional homogenizatio...Lattice materials have demonstrated promising potential in engineering applications owing to their exceptional lightweight,high specific strength,and tunable mechanical properties.However,the traditional homogenization methods based on the classical elasticity theory struggle to accurately describe the non-classical mechanical behaviors of lattice materials,especially when dealing with complex unit-cell geometries featured by non-symmetric configurations or non-single central node connections.In response to this limitation,this study establishes a generalized homogenization model based on the micropolar theory framework,employing Hill's boundary conditions to precisely predict the equivalent moduli of complex lattice materials.By introducing the independent rotational degree of freedom(DOF)characteristic of the micropolar theory,the proposed model successfully overcomes the limitation of conventional methods in accurately describing the asymmetric deformation and scale effects.We initially calculate the constitutive relations of two-dimensional(2D)cross-shaped multi-node chiral lattices and subsequently extend the method to three-dimensional(3D)lattices,successfully predicting the mechanical properties of both traditional and eccentric body-centered cubic(BCC)lattices.The theoretical model is validated through the finite element numerical verification which shows excellent consistency with the theoretical predictions.A further parametric study investigates the influence of geometric parameters,revealing the underlying size-effect mechanism.This paper provides a reliable theoretical tool for the design and property optimization of complex lattice materials.展开更多
Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation...Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation-integrated multimodal photoacoustic/ultrasound(PA/US)imaging framework designed to quantify entheseal oxygen saturation(SO_(2))for assessing entheseal disease activity in PsA.In this cross-sectional study,25 PsA patients underwent bilateral PA/US imaging of 12 entheses,where ultrasound lesions were scored using the Outcome Measures in Rheumatology scoring system,and PA-derived SO_(2) levels,quantified via dual-wavelength PA imaging,were classified into hyperoxia or hypoxia groups using k-means clustering.This approach provides metabolic insights complementary to conventional ultrasonic assessment.A composite score integrating hypoxia with US parameters was validated against clinical disease activity indices(Disease Activity Score 28-C-reactive protein,DAS28-CRP;Disease Activity Index for Psoriatic Arthritis,DAPSA).Among 300 entheses,103(34.3%)exhibited PA positivity,with 40(38.8%)classified as hypoxia.Hypoxia scores independently predicted DAS28-CRP(β=0.618,p=0.001)and DAPSA(β=0.612,p<0:001).The hypoxia-optimized PAUS score demonstrated superior correlation with disease activity indices compared to conventional US(DAS28-CRP:r=0.615,p=0.001 versus r=0.474,p=0.017;DAPSA:r=0.743,p<0:001 versus r=0.567,p=0.003),alongside superior diagnostic accuracy for minimal disease activity(area under the curve,AUC 0.776 versus 0.614,p=0.008)and low disease activity(AUC 0.853 versus 0.772,p=0.009).This multimodal scoring system enhances the stratification of PsA disease activity by providing unique metabolic insights,offering a potential tool for therapeutic monitoring and guiding treat-to-target strategies.展开更多
BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after ESD....BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after ESD.AIM To examine the effectiveness and underlying mechanism of Kangfuxin solution(KFX)in mitigating excessive fibrotic repair of the esophagus post-ESD.METHODS Pigs received KFX at 0.74 mL/kg/d for 21 days after esophageal full circumferential ESD.Endoscopic examinations occurred on days 7 and 21 post-ESD.In vitro,recombinant transforming growth factor(TGF)-β1(5 ng/mL)induced a fibrotic microenvironment in primary esophageal fibroblasts(pEsF).After 24 hours of KFX treatment(at 1.5%,1%,and 0.5%),expression ofα-smooth muscle actin-2(ACTA2),fibronectin(FN),and type collagen I was assessed.Profibrotic signaling was analyzed,including TGF-β1,Smad2/3,and phosphor-smad2/3(p-Smad2/3).RESULTS Compared to the Control group,the groups treated with KFX and prednisolone exhibited reduced esophageal stenosis,lower weight loss rates,and improved food tolerance 21 d after ESD.After treatment,Masson staining revealed thinner and less dense collagen fibers in the submucosal layer.Additionally,the expression of fibrotic effector molecules was notably inhibited.Mechanistically,KFX downregulated the transduction levels of fibrotic functional molecules such as TGF-β1,Smad2/3,and p-Smad2/3.In vitro,pEsF exposed to TGF-β1-induced fibrotic microenvironment displayed increased fibrotic activity,which was reversed by KFX treatment,leading to reduced activation of ACTA2,FN,and collagen I.The 1.5%KFX treatment group showed decreased expression of p-Smad 2/3 in TGF-β1-activated pEsF.CONCLUSION KFX showed promise as a therapeutic option for post-full circumferential esophageal ESD strictures,potentially by suppressing fibroblast fibrotic activity through modulation of the TGF-β1/Smads signaling pathway.展开更多
基金the National Key Research and Development Program of China under contract No.2021YFC2803301the Fundamental Research Funds for the Central Universities,China under contract Nos 2042024kf0037 and 2042022dx0001the Natural Science Foundation of Wuhan under cocntract No.2024040701010030.
文摘Loss of multiyear ice(MYI)is of great importance for Arctic climate and marine systems and can be monitored using active and passive microwave satellite data.In this paper,we describe an upgraded classification algorithm using the data from the scatterometer and radiometer sensors onboard the Chinese Haiyang-2B(HY-2B)satellite to identify MYI and first-year ice(FYI).The proposed method was established based on K-means and fuzzy clustering(K-means+FC)and was used to focus on the transition zone where the ice condition is complex due to the highly commixing of MYI and FYI,leading to the high challenge for accurate classification of sea ice.The K-means algorithm was applied to preliminarily classify MYI using the combination of scatterometer and radiometer data,followed by applying fuzzy clustering to reclassify MYI in the transition zone.The HY-2B K-means+FC results were compared with the ice type products[including the Ocean and Sea Ice Satellite Application Facility(OSI SAF)sea ice type product and the Equal-Area Scalable Earth-Grid sea ice age dataset],and showed agreement in the time series of MYI extent.Intercomparisons in the transition zone indicated that the HY-2B K-means+FC results can identify more old ice than the OSI SAF product,but with an underestimation in identifying second-year ice.Comparisons between K-means and Kmeans+FC results were performed using regional ice charts and Sentinel-1 synthetic aperture radar(SAR)data.By adding fuzzy clustering,the MYI is more consistent with the ice charts,with the overall accuracy(OA)increasing by 0.9%–6.5%.Comparing against SAR images,it is suggested that more scattered MYI floes can be identified by fuzzy clustering,and the OA is increased by about 3%in middle freezing season and 7%–20%in early and late freezing season.
基金supported by the National Natural Science Foundation of China,No.81972073(to HZ)a grant from the Taishan Scholars Program ofShandong Province-Young Taishan Scholars,No.tsqn201909197(to HZ)+1 种基金a grant from Tianjin Key Medical Discipline(Specialty)Construct Project,No.TJYXZDXK-027A(to SF)a grant from Academic Expert International Innovation Summit,No.22JRRCRC00010(to SF).
文摘Ferroptosis,a type of cell death that mainly involves iron metabolism imbalance and lipid peroxidation,is strongly correlated with the phagocytic response caused by bleeding after spinal cord injury.Thus,in this study,bulk RNA sequencing data(GSE47681 and GSE5296)and single-cell RNA sequencing data(GSE162610)were acquired from gene expression databases.We then conducted differential analysis and immune infiltration analysis.Atf3 and Piezo1 were identified as key ferroptosis genes through random forest and least absolute shrinkage and selection operator algorithms.Further analysis of single-cell RNA sequencing data revealed a close relationship between ferroptosis and cell types such as macrophages/microglia and their intrinsic state transition processes.Differences in transcription factor regulation and intercellular communication networks were found in ferroptosis-related cells,confirming the high expression of Atf3 and Piezo1 in these cells.Molecular docking analysis confirmed that the proteins encoded by these genes can bind cycloheximide.In a mouse model of T8 spinal cord injury,low-dose cycloheximide treatment was found to improve neurological function,decrease levels of the pro-inflammatory cytokine inducible nitric oxide synthase,and increase levels of the anti-inflammatory cytokine arginase 1.Correspondingly,the expression of the ferroptosis-related gene Gpx4 increased in macrophages/microglia,while the expression of Acsl4 decreased.Our findings reveal the important role of ferroptosis in the treatment of spinal cord injury,identify the key cell types and genes involved in ferroptosis after spinal cord injury,and validate the efficacy of potential drug therapies,pointing to new directions in the treatment of spinal cord injury.
基金support from the National Natural Science Foundation of China(Grant Nos.42107193,42077245)supported by the Sichuan Science and Technology Program(2025YFNH0008,2025YFNH0004)+1 种基金the State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Independent Research Project(SKLGP2023Z006)the Everest Scientific Research Program 2.0:Research on mechanism and control of glacial lake outburst chain catastrophe in Qinghai-Xizang Plateau based on man-earth coordination perspective.
文摘To investigate the strength degradation characteristics and microscopic damage mechanisms of moraine soil under hydro-thermo-mechanical coupling conditions,a series of X-ray Diffraction(XRD),standard triaxial testing,Scanning Electron Microscopy(SEM),and Nuclear Magnetic Resonance(NMR)experiments were conducted.The mechanical property degradation laws and evolution characteristics of the microscopic pore structure of moraine soil under Freeze-Thaw(F-T)conditions were revealed.After F-T cycles,the stress-strain curves of moraine soil showed a strain-softening trend.In the early stage of F-T cycles(0–5 cycles),the shear strength and elastic modulus exhibited damage rate of approximately 10.33%±0.8%and 16.60%±1.2%,respectively.In the later stage(10–20 cycles),the strength parameters fluctuated slightly and tended to stabilize.The number of F-T cycles was negatively exponentially correlated with cohesion,while showing only slight fluctuation in the internal friction angle,thereby extending the Mohr-Coulomb strength criterion for moraine soil under F-T cycles.The NMR experiments quantitatively characterized the evolution of the internal pore structure of moraine soil under F-T cycles.As the number of F-T cycles increased,fine and micro pores gradually expanded and merged due to the frost-heaving effect during the water-ice phase transition,forming larger pores.The proportion of large and medium pores increased to 59.55%±2.1%(N=20),while that of fine and micro pores decreased to 40.45%±2.1%(N=20).The evolution of pore structure characteristics was essentially completed in the later stage of F-T cycles(10–20 cycles).This study provides a theoretical foundation and technical support for major engineering construction and disaster prevention in the Qinghai-Xizang Plateau.
基金Supported by Army Logistics Department Health Bureau Project,No.QJGYXYJZX-012.
文摘BACKGROUND Due to the dry and cold climate,the obvious temperature difference between day and night,and the low oxygen content of the air in the plateau area,people are prone to upper respiratory tract diseases,and often the condition is prolonged,and the patients are prone to anxiety and uneasiness,which may be related to the harshness of the plateau environment,somatic discomfort due to the lack of oxygen,anxiety about the disease,and other factors.AIM To investigate the effects of cognitive behavioral therapy(CBT)on anxiety,sleep disorders,and hypoxia tolerance in patients with high-altitude respiratory diseases.METHODS A total of 2337 patients with high-altitude-related respiratory diseases treated at our hospital between November 2023 and January 2024 were selected as the study subjects.The subjects’pre-high-altitude residential altitude was approximately 1700 meters.They were divided into two groups.Both groups were given symptomatic treatment,and the control group implemented conventional nursing intervention,while the research group simultaneously conducted CBT intervention;assessed the degree of health knowledge of the two groups,and applied the Hamilton Anxiety Scale and the Pittsburgh Sleep Quality Index to assess the anxiety and sleep quality of the patients before and after the intervention,respectively.It also observed the length and efficiency of sleep,and detected the level of serum hypoxia inducible factor-1α,erythropoietin(EPO)and clinical intervention before and after intervention.EPO levels,and investigated satisfaction with the clinical intervention.RESULTS The rate of excellent health knowledge in the intervention group was 93.64%,which was higher than that in the control group(74.23%;P<0.05).Before the intervention,there was no significant difference in Hamilton Anxiety Scale and Pittsburgh Sleep Quality Index scores between the two groups(P>0.05),and after the intervention,the scores of the study group were significantly lower than those of the control group(P<0.05).There was no significant difference in sleep duration and sleep efficiency between the groups before the intervention(P>0.05),and after the intervention,the scores of the study group were significantly larger than those of the control group(P<0.05).There was no significant difference in serum hypoxia inducible factor-1αand EPO between the two groups before intervention(P>0.05),and both research groups were significantly lower than the control group after intervention(P<0.05).According to the questionnaire survey,the intervention satisfaction of the study group was 95.53%,which was higher than that of the control group(80.14%;P<0.05).CONCLUSION The CBT intervention in the treatment of patients with high-altitude-related respiratory diseases helps improve patients'health knowledge,relieve anxiety,improve sleep quality and hypoxia tolerance,and improve nursing satisfaction.
基金supported by the National Natural Science Foundation of China(No.82473925,81800784,81972117,82472477)the Young Elite Scientists Sponsorship Program by CAST(No.2019QNRC001)+8 种基金the Postdoctoral Science Foundation of China(No.2018M630370)the Key Research and Development Program of Heilongjiang Province(No.GA21C006)the Heilongjiang Postdoctoral Fund for Young Talent Program(No.LBH-TZ1020)the Natural Science Foundation of Heilongjiang Province(No.YQ2020H003)the Research Institution’s Scientific Research Expenses Project of Heilongjiang Province(No.CZKYF2022-1-C006)the Excellent Young Talents Funding(No.2019-QD-11)the CAMS Innovation Fund for Medical Sciences(No.CIFMS 2020-I2M-5-003)the Natural Science Foundation of Jiangsu Province(No.BK20210110)the Program of Science and Technology of Suzhou(No.SYG202119).
文摘Objective Arsenic trioxide(ATO)is a clinically effective anticancer agent used in the treatment of leukemia.However,it exerts adverse effects on non-tumor cells,including bone marrow mesenchymal stem cells(BMSCs).This study aims to investigate the protective role and molecular mechanism of traditional Chinese medicine Astragalus polysaccharides(APS)in mitigating ATO-induced apoptosis in BMSCs.Methods BMSCs exposed to ATO(0.5μmol/L)were treated with APS(20,40,100,and 200μg/mL).Cell viability,proliferation,and migration were assessed by using MTT,EdU staining,Transwell,and scratch wound healing assays.Apoptosis was evaluated via TUNEL assay,Hoechst 33258 staining,and flow cytometry.Intracellular reactive oxygen species(ROS)and mitochondrial membrane potential were measured by using DCFH-DA and JC-1 staining.Apoptotic protein expression was analyzed by Western blotting.Results ATO exposure significantly inhibited the proliferation and migration of BMSCs and induced apoptosis,while APS markedly attenuated the apoptosis of BMSCs induced by ATO,and significantly improved cell proliferation and migration(P<0.01).Mechanistically,APS effectively reduced ATO-induced ROS(P<0.01),while the protein expression of Bcl-2-associated X protein(Bax)and cleaved Caspase-3 was significantly decreased(P<0.05),and the protein expression of Bcl-2 was significantly increased(P<0.01).In addition,APS markedly decreased the protein expression of c-Jun N-terminal kinase(Jnk)and p38 in ATO-activated BMSCs(P<0.05),and significantly decreased the protein expression of p16 and p53(P<0.01),and increased the protein expression of phosphorylated protein kinase B(p-Akt)and phosphorylated extracellular signal-regulated kinase(p-Erk)(P<0.01,0.05).Conclusion Our study reveals that APS exert significant protective effects against ATO-induced apoptosis in BMSCs.The mechanisms involve suppressing ROS generation,maintaining mitochondrial membrane stability,enhancing cell viability,migration,and proliferation,as well as inhibiting Jnk and p38 mitogen-activated protein kinase(p38 MAPK)signaling pathways.The findings highlight potential molecular targets and novel strategies for the clinical prevention and treatment of ATO-related toxicity.
基金supported by the General Project of Humanities and Social Sciences Research of the Ministry of Education of China(24YJA790085)the Science and Technology Innovation Program of Beijing Institute of Technology,China(2024CX01020)。
文摘In China,farmers have increasingly adopted direct-seeded rice(DSR).While various impacts of DSR have been studied,limited evidence exists regarding the effect of DSR adoption on pesticide use.This study examines the impact of DSR adoption on pesticide use utilizing data from a 2018 survey of 982 rice farmers in China's Yangtze River Basin.The endogenous treatment-regression and switching regression models are employed to address self-selection bias.The results indicate that,after accounting for self-selection,DSR adopters spend 401.72 CNY ha^(-1) more on pesticides compared to non-adopters.Although DSR adoption significantly increases the use of insecticides,fungicides and herbicides,its impact is most pronounced for insecticide expenditure and least pronounced for herbicide expenditure.The findings remain robust when altering the dependent variable,truncating the research sample,and modifying the estimation method.Heterogeneous analysis reveals that DSR adoption has a stronger positive impact on pesticide expenditure among farmers below 60 years of age,with at least 6 years of education,and managing rice sown areas less than 2 ha.Based on these findings,this study recommends enhancing complementary techniques for DSR,improving the dissemination of DSR cultivation technologies,and strengthening socialized services.This research provides a comprehensive assessment of DSR's advantages and disadvantages,particularly regarding pesticide use,offering important policy implications for pesticide reduction.
基金supported by foundation of Guizhou Provincial Basic Research Program Natural Sciencel Youth Guidance Project(No.QN[2025]090)Special post of Guizhou University(No.[2024]20).
文摘FAU zeolites have emerged as multifunctional materials with broad applications in catalysis and adsorption,owing to their hierarchical pore architectures,elevated specific surface areas,and adjustable extra-framework cationic sites.This review provides a critical overview of recent advances in FAU zeolite research with emphasis on their roles in environmental pollutant mitigation.A bibliometric analysis was performed to ascertain worldwide research trends,cooperation networks,and principal theme areas.Strategies for synthesis and functionalization,including crystallization pathways,one-pot methods,and post-synthetic modifications,were systematically evaluated for their capacity to tailor structural and physicochemical properties.Environmental applications were discussed in detail,particularly in heavy metal extraction,CO_(2)capture,and catalytic NOx reduction.Despite these advances,challenges persisted,notably restricted chemical stability under extreme pH conditions,scalability obstacles from laboratory to industrial production,and the necessity for enhanced catalytic efficiency.By integrating fundamental understanding with application-oriented perspectives,this review identifies existing knowledge gaps and delineates future directions for the rational design of FAU zeolites toward sustainable environmental remediation.
基金Project supported by the National Natural Science Foundation of China(No.12472077)the supports from Shanghai Gaofeng Project for University Academic Program Development,Fundamental Research Funds for the Central Universities(No.22120240353).
文摘Lattice materials have demonstrated promising potential in engineering applications owing to their exceptional lightweight,high specific strength,and tunable mechanical properties.However,the traditional homogenization methods based on the classical elasticity theory struggle to accurately describe the non-classical mechanical behaviors of lattice materials,especially when dealing with complex unit-cell geometries featured by non-symmetric configurations or non-single central node connections.In response to this limitation,this study establishes a generalized homogenization model based on the micropolar theory framework,employing Hill's boundary conditions to precisely predict the equivalent moduli of complex lattice materials.By introducing the independent rotational degree of freedom(DOF)characteristic of the micropolar theory,the proposed model successfully overcomes the limitation of conventional methods in accurately describing the asymmetric deformation and scale effects.We initially calculate the constitutive relations of two-dimensional(2D)cross-shaped multi-node chiral lattices and subsequently extend the method to three-dimensional(3D)lattices,successfully predicting the mechanical properties of both traditional and eccentric body-centered cubic(BCC)lattices.The theoretical model is validated through the finite element numerical verification which shows excellent consistency with the theoretical predictions.A further parametric study investigates the influence of geometric parameters,revealing the underlying size-effect mechanism.This paper provides a reliable theoretical tool for the design and property optimization of complex lattice materials.
基金supported by the National Natural Science Foundation of China(62325112)the National Key Research and Development Program of China(2023YFC2411700,2023YFC2411705)+2 种基金the National Natural Science Foundation of China(U22A2023)the National High-Level Hospital Clinical Research Funding(2022-PUMCH-C-009,2022-PUMCH-B-064,2022-PUMCH-D-002)the National Basic Research Program of China(973 Program,2014CB541801).
文摘Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation-integrated multimodal photoacoustic/ultrasound(PA/US)imaging framework designed to quantify entheseal oxygen saturation(SO_(2))for assessing entheseal disease activity in PsA.In this cross-sectional study,25 PsA patients underwent bilateral PA/US imaging of 12 entheses,where ultrasound lesions were scored using the Outcome Measures in Rheumatology scoring system,and PA-derived SO_(2) levels,quantified via dual-wavelength PA imaging,were classified into hyperoxia or hypoxia groups using k-means clustering.This approach provides metabolic insights complementary to conventional ultrasonic assessment.A composite score integrating hypoxia with US parameters was validated against clinical disease activity indices(Disease Activity Score 28-C-reactive protein,DAS28-CRP;Disease Activity Index for Psoriatic Arthritis,DAPSA).Among 300 entheses,103(34.3%)exhibited PA positivity,with 40(38.8%)classified as hypoxia.Hypoxia scores independently predicted DAS28-CRP(β=0.618,p=0.001)and DAPSA(β=0.612,p<0:001).The hypoxia-optimized PAUS score demonstrated superior correlation with disease activity indices compared to conventional US(DAS28-CRP:r=0.615,p=0.001 versus r=0.474,p=0.017;DAPSA:r=0.743,p<0:001 versus r=0.567,p=0.003),alongside superior diagnostic accuracy for minimal disease activity(area under the curve,AUC 0.776 versus 0.614,p=0.008)and low disease activity(AUC 0.853 versus 0.772,p=0.009).This multimodal scoring system enhances the stratification of PsA disease activity by providing unique metabolic insights,offering a potential tool for therapeutic monitoring and guiding treat-to-target strategies.
基金Supported by Science and Technology Department of Sichuan Province,No.2020YFS0376National Natural Science Foundation of China,No.81900599Science and Technology Program of Hospital of TCM,Southwest Medical University,No.2022-CXTD-01.
文摘BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after ESD.AIM To examine the effectiveness and underlying mechanism of Kangfuxin solution(KFX)in mitigating excessive fibrotic repair of the esophagus post-ESD.METHODS Pigs received KFX at 0.74 mL/kg/d for 21 days after esophageal full circumferential ESD.Endoscopic examinations occurred on days 7 and 21 post-ESD.In vitro,recombinant transforming growth factor(TGF)-β1(5 ng/mL)induced a fibrotic microenvironment in primary esophageal fibroblasts(pEsF).After 24 hours of KFX treatment(at 1.5%,1%,and 0.5%),expression ofα-smooth muscle actin-2(ACTA2),fibronectin(FN),and type collagen I was assessed.Profibrotic signaling was analyzed,including TGF-β1,Smad2/3,and phosphor-smad2/3(p-Smad2/3).RESULTS Compared to the Control group,the groups treated with KFX and prednisolone exhibited reduced esophageal stenosis,lower weight loss rates,and improved food tolerance 21 d after ESD.After treatment,Masson staining revealed thinner and less dense collagen fibers in the submucosal layer.Additionally,the expression of fibrotic effector molecules was notably inhibited.Mechanistically,KFX downregulated the transduction levels of fibrotic functional molecules such as TGF-β1,Smad2/3,and p-Smad2/3.In vitro,pEsF exposed to TGF-β1-induced fibrotic microenvironment displayed increased fibrotic activity,which was reversed by KFX treatment,leading to reduced activation of ACTA2,FN,and collagen I.The 1.5%KFX treatment group showed decreased expression of p-Smad 2/3 in TGF-β1-activated pEsF.CONCLUSION KFX showed promise as a therapeutic option for post-full circumferential esophageal ESD strictures,potentially by suppressing fibroblast fibrotic activity through modulation of the TGF-β1/Smads signaling pathway.
