Highly active and stable FeOOH cocatalysts are essential for achieving optimal performance of BiVO_(4)(BVO)photoanodes.Despite offering remarkable structural stability,widely used thick FeOOH cocatalysts often suffer ...Highly active and stable FeOOH cocatalysts are essential for achieving optimal performance of BiVO_(4)(BVO)photoanodes.Despite offering remarkable structural stability,widely used thick FeOOH cocatalysts often suffer from insufficient hole transport capability,which hinders the overall activity.The present study demonstrates that a simple photoetching strategy is able to introduce gradient distributed oxygen vacancies(GO_(V))in the thick FeOOH layer and significantly enhances the photogenerated holes transport dynamics.The incorporation of GO_(V)within FeOOH not only realizes the“relay transport”of photogenerated hole through the progressive upward shift of the valence band in the spatial distribution,but also provides abundant oxidation active sites by efficient hole trapping.These improvements effectively improve the oxygen evolution reaction(OER)activities and mitigate photocorrosion by the instantaneous hole extraction.Consequently,the FeOOH-GO_(V)layer enables the BVO/FeOOH-GO_(V)photoanode to achieve an impressive photocurrent density of 5.37 mA cm^(-2)and a robust operational stability up to 160 h at 1.23 VRHE,setting new benchmarks for current density and stability in FeOOH-based BVO photoanodes.This work provides an effective avenue to optimize OER cocatalysts for constructing highly efficient and stable photoelectrochemical water splitting devices.展开更多
The Wide Field Survey Telescope(WFST)is located at 4200 m on Saishiteng Mountain in Lenghu,Qinghai Province,China.It features a primary mirror with a diameter of 2.5 m and a camera equipped with nine CCDs,providing a ...The Wide Field Survey Telescope(WFST)is located at 4200 m on Saishiteng Mountain in Lenghu,Qinghai Province,China.It features a primary mirror with a diameter of 2.5 m and a camera equipped with nine CCDs,providing a wide field of view of approximately 3×3 square degrees.Calibration parameters are essential to ensure the precision of astrometric observations with the WFST.These parameters are derived from geometric distortion(GD)and gaps through astrometric modeling and are subsequently validated via the Yao’An High Precision Telescope(YAHPT).The GD solutions show maximum distortions between 1.18 and 10.29 pixels for the WFST chips,with central chips exhibiting lower distortion.After applying the GD correction,the precision of the WFST reaches 4 mas.The interchip gaps of the WFST range from 1.922 mm to 7.765 mm,corresponding to 10μm/pixel,aligning with the design and measurements.The calibrated parameters guarantee that the WFST can perform highly accurate astrometric measurements.Furthermore,as the WFST undergoes updates,the parameter model remains consistently applicable.展开更多
Background:Ferroptosis is a type of regulated cell death characterized by iron-dependent lipid peroxidation,which has been linked to tumor progression and therapeutic resistance.However,the contribution of lactate met...Background:Ferroptosis is a type of regulated cell death characterized by iron-dependent lipid peroxidation,which has been linked to tumor progression and therapeutic resistance.However,the contribution of lactate metabolism and its receptor,hydroxycarboxylic acid receptor 1(HCAR1),in ferroptosis regulation in gastric cancer(GC)remains poorly understood.Focusing specifically on its effects on cell proliferation,ferroptosis regulation,and the disruption of lactate-mediatedmetabolic pathways,the study aimed to clarify the role ofHCAR1 in GC progression.Methods:Bioinformatics analysis identified prognostic genes associated with ferroptosis in GC.Receiver operating characteristic(ROC)curves were generated to assess the diagnostic potential of the predictive genes.The biological role of HCAR1 was investigated through gain and loss-of-function experiments in GC cell lines,followed by assessments of cell viability,oxidative stress indicators,gene/protein expression,and ferroptosis sensitivity under lactate stimulation or HCAR1modulation.Results:HCAR1 was significantly upregulated in GC tissues and linked to poor patient outcomes.Silencing HCAR1 inhibited GC cell growth and induced ferroptosis,as shown by increased levels of reactive oxygen species(ROS)and malondialdehyde(MDA),along with decreased expression of solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4).Conversely,HCAR1 overexpression or exposure to extracellular lactate inhibited ferroptosis and activated antioxidant defenses.Mechanistically,lactate activation of HCAR1 increases ATP levels,which in turn inactivates AMP-activated protein kinase(AMPK).It also upregulates stearoyl-CoA desaturase 1(SCD1)through the sterol regulatory element binding protein 1(SREBP1)signaling pathway.Blocking HCAR1 reversed these effects and restored ferroptosis sensitivity.Conclusion:HCAR1 mediates lactate-driven ferroptosis resistance in GC through the AMPK-SCD1 signaling pathway.Targeting the HCAR1-lactate axis may offer a promising strategy for overcoming metabolic adaptation and improving GC treatment outcomes.展开更多
BACKGROUND In recent years,the rapid development of artificial intelligence(AI)in hepatobiliary surgery research has led to an increase in articles exploring its benefits.We performed a bibliometric analysis of AI app...BACKGROUND In recent years,the rapid development of artificial intelligence(AI)in hepatobiliary surgery research has led to an increase in articles exploring its benefits.We performed a bibliometric analysis of AI applications in hepatobiliary surgery to better delineate the contemporary state of AI application in hepatobiliary surgery and potential future trajectories.AIM To provide clinical practitioners with a reliable reference point.It offers a detailed overview of the development of AI in hepatobiliary surgery by systematically examining the contributions of authors,countries,institutions,journals,and keywords in this domain over the last 10 years.METHODS The academic resources utilized in this study were obtained from the Web of Science Core Collection database.The search results were subsequently integrated and imported into CiteSpace and VOSviewer software for the purpose of visual analysis.RESULTS The study analyzed 2552 publications during 2014–2024.These publications collectively garnered 32628 citations,averaging 15.66 citations per paper.The top contributor to this field was China.The USA had the highest citation count.The author with the highest citation count was Summers RM.In terms of the number of articles published,the leading journals were Medical Physics.Excluding the subject search terms,the most frequently used keywords included“classification”,“CT and“diagnosis”.CONCLUSION This bibliometric analysis indicates that research on AI in hepatobiliary surgery has entered a period of rapid development,particularly in the domain of disease imaging diagnostics.展开更多
Objective:To investigate the application effects of intelligent guidance systems in optimizing health check-up process management.Methods:A total of 400 examinees who underwent physical examinations at the hospital’s...Objective:To investigate the application effects of intelligent guidance systems in optimizing health check-up process management.Methods:A total of 400 examinees who underwent physical examinations at the hospital’s Health Management Center from January to December 2024 were randomly divided into a control group(200 cases)and an observation group(200 cases).The control group used traditional manual guidance methods,while the observation group employed the intelligent guidance system.The study compared two groups in terms of completion time,waiting time for each procedure,check-up efficiency scores,examinee satisfaction,and report issuance time.Results:The overall examination time in the observation group(85.3±12.7 minutes)was significantly shorter than that in the control group(142.6±18.5 minutes)(P<0.01);average waiting time per procedure decreased by 62.4%;check-up efficiency scores(8.9±0.8 points)were significantly higher than those in the control group(5.2±1.1 points)(P<0.01);satisfaction reached 96.5%,significantly higher than the control group’s 78.0%(P<0.01);and report issuance time was advanced by 1.5 days.Conclusion:Intelligent guidance systems can significantly optimize check-up processes,improve work efficiency,and examinee satisfaction,demonstrating significant clinical application value.展开更多
Various therapeutic modalities have been engineered for lung cancer treatment,but their clinic application is severely impeded by the poor therapy efficiency and immunosuppressive microenvironment.Herein,we fabricated...Various therapeutic modalities have been engineered for lung cancer treatment,but their clinic application is severely impeded by the poor therapy efficiency and immunosuppressive microenvironment.Herein,we fabricated a library of small molecule redox-labile nanoparticles(NPs)(i.e.,diPTX-2C NPs,diPTX-2S NPs,and diPTX-2Se NPs)by the self-assembly of dimer paclitaxel(PTX)prodrug,and then utilized these NPs with the traditional Chinese medicine(TCM)Qi-Yu-San-Long-Fang(Q)for effective chemoimmunotherapy on Lewis lung carcinoma(LLC)-bearing mice models.Under the high concentration of glutathione(GSH)and H_(2)O_(2),diPTX-2Se NPs could specifically release PTX in cancer cells and exert a higher selectivity and toxicity than normal cells.In LLC tumor-bearing mice,oral administration of Q not only effectively downregulated programmed death ligand-1(PD-L1)expression,but also remodeled the immunosuppressive tumor immune microenvironment via the increase of CD4^(+)T and CD8^(+)T cell proportion and the repolarization of M2 into M1 macrophages in tumor tissues,collectively achieving superior synergistic treatment outcomes in combination with intravenous PTX prodrug NPs.Besides,we found that the combination regimen also demonstrated excellent chemoimmunotherapeutic performances on low-dose small established tumor and high-dose large established tumor models.This study may shed light on the potent utilization of Chinese and Western-integrative strategy for efficient tumor chemoimmunotherapy.展开更多
Regional turbofan aircraft,which are used for medium-short distances,have a heightened risk of high-altitude Wake Vortices(VV)because of their tail-mounted engines and high horizontal tail configurations.For some regi...Regional turbofan aircraft,which are used for medium-short distances,have a heightened risk of high-altitude Wake Vortices(VV)because of their tail-mounted engines and high horizontal tail configurations.For some regional medium-short-range turbofan aircraft,this threat is higher than that for conventionally designed aircraft.To analyze the flight safety of turbofan aircraft during cruise,this study developed a model to assess wake vortex encounters based on evolutionary high-altitude wake flow patterns.First,the high-altitude wake vortex aircraft dissipation patterns were analyzed by combining Quick Access Recorder(QAR)flight data with the wake vortex evolution model.Then,to consider the uniqueness of the medium-short-range turbofan aircraft,the severity of the wake vortex encounters was simulated using an induced roll moment coefficient.The proposed high-altitude wake vortex encounter model was able to identify and assess the highaltitude wake vortex changes,the bearing moments at different altitudes,and the atmospheric pressure conditions.Using the latest wake separation standards from the International Civil Aviation Organization(ICAO),acceptable safety wake intervals for follower aircraft in different scenarios were determined for the safety assessment.The results indicate that compared to mid and low altitudes,the high-altitude aircraft wake vortex dissipation rate is faster,the ultimate bearing moment is weaker,and the roll moment coefficient is higher,which confirm that there is elevated wake vortex encounter severity for regional turbofan aircraft.As safety is found to deteriorate when encountering wake vortices at altitudes higher than 8 km,new medium-short-range turbofan regional aircraft require higher safety margins than the latest wake separation standards.展开更多
Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence i...Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence immunoassay system was designed.We encapsulated carbon dots with a high fluorescence quantum yield(QY=92.5%)inside silicon nanocapsules to serve as fluorescent markers.These markers were then integrated with the streptavidin(SA)-biotin biomagnification system and immunomagnetic separation technology for the sensitive detection of GFAP.Based on the signal cascade amplification effect of the silicon nanocapsules and SA-biotin,the fluorescence signal of the SA-biotin-modified immunofluorescence nanocapsules increased 3.6-fold compared to the carbon dot-based immunoprobe.The fluorescence immunoassay system was constructed for GFAP using SA-biotin-modified immunocapsules as the sensing probe and immunomagnetic nanoparticles as the immunorecognition probe.The fluorescence immunoassay system can specifically and ultra-sensitively quantify GFAP in blood samples,with a detection range of 10 pg/mL–10 ng/mL and detection limits of 3.2 pg/mL(serum)and 3.6 pg/mL(plasma).Moreover,the fluorescence immunoassay system exhibited prominent recoveries of 99.4%–100.4%(phosphate buffered saline),96%–102.6%(serum),and 93.2%–110.2%(plasma),with favorable specificity and excellent stabilization.The novel fluorescence immunoassay system provides a new approach to the clinical analysis of GFAP and may serve as a potential tool for screening and diagnosing TBI.展开更多
The adsorption of pollutants can not only promote the direct surface reaction,but also modify the catalyst itself to improve its photoelectric characteristics,which is rarely studied for water treatment with inorganic...The adsorption of pollutants can not only promote the direct surface reaction,but also modify the catalyst itself to improve its photoelectric characteristics,which is rarely studied for water treatment with inorganic photocatalyst.A highly crystalline BiOBr(c-BiOBr)was synthesized by a two-step preparation process.Owing to the calcination,the highly crystalline enhanced the interface interaction between pollutant and c-BiOBr.The complex of organic pollutant and[Bi_(2)O_(2)]^(2+)could promote the active electron transfer from the adsorbed pollutant to c-BiOBr for the direct pollutant degradation by holes(h^(+)).Moreover,the pollutant adsorption actually modified c-BiOBr and promoted more unpaired electrons,which would coupling with the photoexcitation to promote generate more O_(2)^(•-).The molecular modification effect derived from pollutant adsorption significantly improved the removal of pollutants.This work strongly deepens the understanding of the molecularmodification effect from the pollutant adsorption and develops a novel and efficient approach for water treatment.展开更多
Objective:To investigate the impact of programmed harmonious nursing combined with hierarchical management on nursing quality and satisfaction in a health management center.Methods:A total of 100 patients who received...Objective:To investigate the impact of programmed harmonious nursing combined with hierarchical management on nursing quality and satisfaction in a health management center.Methods:A total of 100 patients who received care at this health management center from January 2024 to January 2025 were selected as subjects.Using a random number table method,they were divided into an observation group(n=50)and a control group(n=50).The control group followed traditional methods,while the observation group integrated programmed harmonious nursing with hierarchical management.Comparative analysis was conducted on nursing quality scores,adverse event occurrence rates,and patient satisfaction between the two groups.Results:The observation group showed significantly improved nursing quality scores(P<0.05)and markedly reduced incidence of adverse events(P<0.05),with statistically significant differences compared to pre-treatment conditions(P<0.05).Conclusion:The combination of hierarchical management and programmed harmonious nursing demonstrates effectiveness in enhancing medical service quality,reducing adverse reactions,and improving patient satisfaction—a method worthy of promotion.展开更多
AIM： To investigate the role of Livin and vascular endothelial growth factor （VEGF） in human esophageal carcinoma, and analyze its relationship to clinical stages.METHODS： Expression of Livin in fresh esophageal c...AIM： To investigate the role of Livin and vascular endothelial growth factor （VEGF） in human esophageal carcinoma, and analyze its relationship to clinical stages.METHODS： Expression of Livin in fresh esophageal cancer tissues was detected by immunohistochemistry （IHC）, Western blotting and reverse transcriptasepolyrnerase chain reaction （RT-PCR）, and VEGF by Western blotting and RT-PCR. All statistical analyses were performed by SPSS version 11.0. RESULTS： Livin positivity was also significantly correlated with tumor stages, increasing with tumor progression. Expression of Livin and VEGF increased with the process of esophageal carcinoma. In the fourth clinical stage, expression of Livin and VEGF was the most significant. Expression of Livin was positively correlated with VEGF. CONCLUSION： Over-expression of Livin and VEGF contributes to the pathogenesis of esophageal carcinoma.展开更多
BACKGROUND Although total or subtotal colectomy for slow-transit constipation(STC)has been proven to be a definite treatment,the associated defecation function and quality of life(QOL)are rarely studied.AIM To evaluat...BACKGROUND Although total or subtotal colectomy for slow-transit constipation(STC)has been proven to be a definite treatment,the associated defecation function and quality of life(QOL)are rarely studied.AIM To evaluate the effectiveness of surgery for STC regarding defecation function and QOL.METHODS From March 2013 to September 2017,30 patients undergoing surgery for STC in our department were analyzed.Preoperative,intra-operative,and postoperative 3-mo,6-mo,1-year,and 2-year follow-up details were recorded.Defecation function was assessed by bowel movements,abdominal pain,bloating,straining,laxative,enema use,diarrhea,and the Wexner constipation and incontinence scales.QOL was evaluated using the gastrointestinal QOL index and the 36-item short form survey.RESULTS The majority of patients(93.1%,27/29)stated that they benefited from the operation at the 2-year follow-up.At each time point of the follow-up,the number of bowel movements per week significantly increased compared with that of the preoperative conditions(P<0.05).Similarly,compared with the preoperative values,a marked decline was observed in bloating,straining,laxative,and enema use at each time point of the follow-up(P<0.05).Postoperative diarrhea could be controlled effectively and notably improved at the 2-year follow-up.The Wexner incontinence scores at 6-mo,1-year,and 2-year were notably lower than those at the 3-mo follow-up(P<0.05).Compared with those of the preoperative findings,the Wexner constipation scores significantly decreased following surgery(P<0.05).Thus,it was reasonable to find that the gastrointestinal QOL index scores clearly increase(P<0.05)and that the 36-item short form survey results displayed considerable improvements in six spheres(role physical,role emotional,physical pain,vitality,mental health,and general health)following surgery.CONCLUSION Total or subtotal colectomy for STC is not only effective in alleviating constipation-related symptoms but also in enhancing patients’QOL.展开更多
BACKGROUND It is important to differentiate benign and malignant focal liver lesions(FLLs)accurately.Despite the wide use and acceptance of shear wave elastography(SWE),its value for assessing the elasticity of FLLs a...BACKGROUND It is important to differentiate benign and malignant focal liver lesions(FLLs)accurately.Despite the wide use and acceptance of shear wave elastography(SWE),its value for assessing the elasticity of FLLs and differentiating benign and malignant FLLs is still investigational.Previous studies of SWE for FLLs used mean elasticity as the parameter to reflect the stiffness of FLLs.Considering the inhomogeneity of tumor stiffness,maximal elasticity(Emax)might be the suitable parameter to reflect the stiffness of FLLs and to differentiate malignant FLLs from benign ones.AIM To explore the value of SWE with Emax in differential diagnosis of solid FLLs.METHODS We included 104 solid FLLs in 95 patients and 50 healthy volunteers.All the subjects were examined using conventional ultrasound(US)and virtual touch tissue quantification(VTQ)imaging.A diagnosis of benign or malignant FLL was made using conventional US.Ten VTQ values were acquired after 10 consecutive measurements for each FLL and each normal liver,and the largest value was recorded as Emax.RESULTS There were 56 cases of malignant FLLs and 48 cases of benign FLLs in this study.Emax of malignant FLLs(3.29±0.88 m/s)was significantly higher than that of benign FLLs(1.30±0.46 m/s,P<0.01)and that of livers in healthy volunteers(1.15±0.17 m/s,P<0.01).The cut-off point of Emax was 1.945,and the area under the curve was 0.978.The sensitivity and specificity of Emax were 92.9%and 91.7%,respectively,higher(but not significantly)than those of conventional US(80.4%for sensitivity and 81.3%for specificity).Combined diagnosis of conventional US and Emax using parallel testing improved the sensitivity to 100%with specificity of 75%.CONCLUSION SWE is a convenient and easy method to obtain accurate stiffness information of solid FLLs.Emax is useful for differential diagnosis of FLLs,especially in combination with conventional US.展开更多
Objectives To investigate the overall prevalence of cardiovascular disease （CVD） in subjects hospitalized for chronic obstructive pulmonary disease （COPD）,and explore the prevalence of the major CVD complications ...Objectives To investigate the overall prevalence of cardiovascular disease （CVD） in subjects hospitalized for chronic obstructive pulmonary disease （COPD）,and explore the prevalence of the major CVD complications and trends in patients with COPD over a 10-year period.Methods Medical records in the PLA General Hospital,Beijing Union Medical College Hospital,and Beijing Hospital from 2000/01/01 to 2010/03/03 were retrospectively reviewed.A total of 4960 patients with COPD were reviewed in the study （3570 males,mean age,72.2 ± 10.5 years; 1390 females,mean age,72.0 ± 10.4 years）.Results The prevalence of CVD in COPD patients was 51.7％.The three most prevalent CVDs were ischemic heart disease （28.9％）,heart failure （19.6％）,and arrhythmia （12.6％）.During the 10-year study period,the prevalence of various CVDs in COPD patients showed a gradual increasing trend with increasing age.There was higher morbidity due to ischemic heart disease （P ＜ 0.01） in male COPD patients than in the female counterparts.However,heart failure （P ＜ 0.01）and hypertension （P ＜ 0.01） occurred less frequently in male COPD patients than in female COPD patients.Furthermore,the prevalence of ischemic heart disease decreased year by year.In addition to heart failure,various types of CVD complications in COPD patients tended to occur in younger subjects.The prevalence of all major types of CVD in women tended to increase year by year.Conclusions The prevalence of CVD in patients hospitalized for COPD in Beijing was high.Age,sex and CVD trends,as well as life style changes,should be considered when prevention and control strategies are formulated.展开更多
The accurate fault-cause identification for overhead transmission lines supports the operation and maintenance personnel in formulating targeted maintenance strategies and shortening the time of inspecting faulty line...The accurate fault-cause identification for overhead transmission lines supports the operation and maintenance personnel in formulating targeted maintenance strategies and shortening the time of inspecting faulty lines.With the goal of achieving“carbon peak and carbon neutrality”,the schemes for clean energy generation have rapidly developed.Moreover,new energy-consuming equipment has been widely connected to the power grid,and the operating characteristics of the power system have significantly changed.Consequently,these have impacted traditional fault identification methods.Based on the time-frequency characteristics of the fault waveform,new energy-related parameters,and deep learning model,this study proposes a fault identification method suitable for scenarios where a high proportion of new energy is connected to the power grid.Ten parameters related to the causes of transmission line fault and new energy connection scenarios are selected as model characteristic parameters.Further,a fault identification model based on adaptive deep belief networks was constructed,and its effect was verified by field data.展开更多
基金supported by the State Key Laboratory of Solidification Processing in NWPU(SKLSP202407)the National Natural Science Foundation of China(52402130)+2 种基金the Natural Science Basis Research Plan in Shaanxi Province of China(2024JC-YBQN-0384)the Shaanxi Science and Technology Innovation Team(2023-CX-TD-44)the National Natural Science Foundation of China(52301015).
文摘Highly active and stable FeOOH cocatalysts are essential for achieving optimal performance of BiVO_(4)(BVO)photoanodes.Despite offering remarkable structural stability,widely used thick FeOOH cocatalysts often suffer from insufficient hole transport capability,which hinders the overall activity.The present study demonstrates that a simple photoetching strategy is able to introduce gradient distributed oxygen vacancies(GO_(V))in the thick FeOOH layer and significantly enhances the photogenerated holes transport dynamics.The incorporation of GO_(V)within FeOOH not only realizes the“relay transport”of photogenerated hole through the progressive upward shift of the valence band in the spatial distribution,but also provides abundant oxidation active sites by efficient hole trapping.These improvements effectively improve the oxygen evolution reaction(OER)activities and mitigate photocorrosion by the instantaneous hole extraction.Consequently,the FeOOH-GO_(V)layer enables the BVO/FeOOH-GO_(V)photoanode to achieve an impressive photocurrent density of 5.37 mA cm^(-2)and a robust operational stability up to 160 h at 1.23 VRHE,setting new benchmarks for current density and stability in FeOOH-based BVO photoanodes.This work provides an effective avenue to optimize OER cocatalysts for constructing highly efficient and stable photoelectrochemical water splitting devices.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA0350300)the National Natural Science Foundation of China(12203105,12103091,62394351,12073008)the China Manned Space Project(CMS-CSST-2021-A12,CMS-CSST-2021-B10).
文摘The Wide Field Survey Telescope(WFST)is located at 4200 m on Saishiteng Mountain in Lenghu,Qinghai Province,China.It features a primary mirror with a diameter of 2.5 m and a camera equipped with nine CCDs,providing a wide field of view of approximately 3×3 square degrees.Calibration parameters are essential to ensure the precision of astrometric observations with the WFST.These parameters are derived from geometric distortion(GD)and gaps through astrometric modeling and are subsequently validated via the Yao’An High Precision Telescope(YAHPT).The GD solutions show maximum distortions between 1.18 and 10.29 pixels for the WFST chips,with central chips exhibiting lower distortion.After applying the GD correction,the precision of the WFST reaches 4 mas.The interchip gaps of the WFST range from 1.922 mm to 7.765 mm,corresponding to 10μm/pixel,aligning with the design and measurements.The calibrated parameters guarantee that the WFST can perform highly accurate astrometric measurements.Furthermore,as the WFST undergoes updates,the parameter model remains consistently applicable.
文摘Background:Ferroptosis is a type of regulated cell death characterized by iron-dependent lipid peroxidation,which has been linked to tumor progression and therapeutic resistance.However,the contribution of lactate metabolism and its receptor,hydroxycarboxylic acid receptor 1(HCAR1),in ferroptosis regulation in gastric cancer(GC)remains poorly understood.Focusing specifically on its effects on cell proliferation,ferroptosis regulation,and the disruption of lactate-mediatedmetabolic pathways,the study aimed to clarify the role ofHCAR1 in GC progression.Methods:Bioinformatics analysis identified prognostic genes associated with ferroptosis in GC.Receiver operating characteristic(ROC)curves were generated to assess the diagnostic potential of the predictive genes.The biological role of HCAR1 was investigated through gain and loss-of-function experiments in GC cell lines,followed by assessments of cell viability,oxidative stress indicators,gene/protein expression,and ferroptosis sensitivity under lactate stimulation or HCAR1modulation.Results:HCAR1 was significantly upregulated in GC tissues and linked to poor patient outcomes.Silencing HCAR1 inhibited GC cell growth and induced ferroptosis,as shown by increased levels of reactive oxygen species(ROS)and malondialdehyde(MDA),along with decreased expression of solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4).Conversely,HCAR1 overexpression or exposure to extracellular lactate inhibited ferroptosis and activated antioxidant defenses.Mechanistically,lactate activation of HCAR1 increases ATP levels,which in turn inactivates AMP-activated protein kinase(AMPK).It also upregulates stearoyl-CoA desaturase 1(SCD1)through the sterol regulatory element binding protein 1(SREBP1)signaling pathway.Blocking HCAR1 reversed these effects and restored ferroptosis sensitivity.Conclusion:HCAR1 mediates lactate-driven ferroptosis resistance in GC through the AMPK-SCD1 signaling pathway.Targeting the HCAR1-lactate axis may offer a promising strategy for overcoming metabolic adaptation and improving GC treatment outcomes.
基金Supported by the National Key Research and Development Program of China,No.2023YFC3605202Project of the Central Government in Guidance of Local Science and Technology Development,No.2024ZYD0270and Luzhou Science and Technology Bureau,No.2024SYF156.
文摘BACKGROUND In recent years,the rapid development of artificial intelligence(AI)in hepatobiliary surgery research has led to an increase in articles exploring its benefits.We performed a bibliometric analysis of AI applications in hepatobiliary surgery to better delineate the contemporary state of AI application in hepatobiliary surgery and potential future trajectories.AIM To provide clinical practitioners with a reliable reference point.It offers a detailed overview of the development of AI in hepatobiliary surgery by systematically examining the contributions of authors,countries,institutions,journals,and keywords in this domain over the last 10 years.METHODS The academic resources utilized in this study were obtained from the Web of Science Core Collection database.The search results were subsequently integrated and imported into CiteSpace and VOSviewer software for the purpose of visual analysis.RESULTS The study analyzed 2552 publications during 2014–2024.These publications collectively garnered 32628 citations,averaging 15.66 citations per paper.The top contributor to this field was China.The USA had the highest citation count.The author with the highest citation count was Summers RM.In terms of the number of articles published,the leading journals were Medical Physics.Excluding the subject search terms,the most frequently used keywords included“classification”,“CT and“diagnosis”.CONCLUSION This bibliometric analysis indicates that research on AI in hepatobiliary surgery has entered a period of rapid development,particularly in the domain of disease imaging diagnostics.
文摘Objective:To investigate the application effects of intelligent guidance systems in optimizing health check-up process management.Methods:A total of 400 examinees who underwent physical examinations at the hospital’s Health Management Center from January to December 2024 were randomly divided into a control group(200 cases)and an observation group(200 cases).The control group used traditional manual guidance methods,while the observation group employed the intelligent guidance system.The study compared two groups in terms of completion time,waiting time for each procedure,check-up efficiency scores,examinee satisfaction,and report issuance time.Results:The overall examination time in the observation group(85.3±12.7 minutes)was significantly shorter than that in the control group(142.6±18.5 minutes)(P<0.01);average waiting time per procedure decreased by 62.4%;check-up efficiency scores(8.9±0.8 points)were significantly higher than those in the control group(5.2±1.1 points)(P<0.01);satisfaction reached 96.5%,significantly higher than the control group’s 78.0%(P<0.01);and report issuance time was advanced by 1.5 days.Conclusion:Intelligent guidance systems can significantly optimize check-up processes,improve work efficiency,and examinee satisfaction,demonstrating significant clinical application value.
基金supported by the Young Elite Scientists Sponsorship Program by China Association of Chinese Medicine(CACM),China(Grant No.:2022-QNRC2-B12)the Key Project of Anhui Province Department of Education,China(Grant Nos.:2023AH030070,2024AH051040,and 2022AH050528)+1 种基金Anhui Province Key Laboratory,China(Grant Nos.:KFKT202305,2024ZYFBAHKLA11,and 2024ZYFBAHKLA15)Greater Health Research Institute of Hefei Comprehensive National Science Center,China(Grant No.:2023CXMMTCM005).
文摘Various therapeutic modalities have been engineered for lung cancer treatment,but their clinic application is severely impeded by the poor therapy efficiency and immunosuppressive microenvironment.Herein,we fabricated a library of small molecule redox-labile nanoparticles(NPs)(i.e.,diPTX-2C NPs,diPTX-2S NPs,and diPTX-2Se NPs)by the self-assembly of dimer paclitaxel(PTX)prodrug,and then utilized these NPs with the traditional Chinese medicine(TCM)Qi-Yu-San-Long-Fang(Q)for effective chemoimmunotherapy on Lewis lung carcinoma(LLC)-bearing mice models.Under the high concentration of glutathione(GSH)and H_(2)O_(2),diPTX-2Se NPs could specifically release PTX in cancer cells and exert a higher selectivity and toxicity than normal cells.In LLC tumor-bearing mice,oral administration of Q not only effectively downregulated programmed death ligand-1(PD-L1)expression,but also remodeled the immunosuppressive tumor immune microenvironment via the increase of CD4^(+)T and CD8^(+)T cell proportion and the repolarization of M2 into M1 macrophages in tumor tissues,collectively achieving superior synergistic treatment outcomes in combination with intravenous PTX prodrug NPs.Besides,we found that the combination regimen also demonstrated excellent chemoimmunotherapeutic performances on low-dose small established tumor and high-dose large established tumor models.This study may shed light on the potent utilization of Chinese and Western-integrative strategy for efficient tumor chemoimmunotherapy.
基金supported by the National Natural Science Foundation of China(Nos.U2333209,U1733203)the National Key R&D Program of China(No.2021YFF0603904)the Civil Aviation Administration of China(No.AQ20200019)。
文摘Regional turbofan aircraft,which are used for medium-short distances,have a heightened risk of high-altitude Wake Vortices(VV)because of their tail-mounted engines and high horizontal tail configurations.For some regional medium-short-range turbofan aircraft,this threat is higher than that for conventionally designed aircraft.To analyze the flight safety of turbofan aircraft during cruise,this study developed a model to assess wake vortex encounters based on evolutionary high-altitude wake flow patterns.First,the high-altitude wake vortex aircraft dissipation patterns were analyzed by combining Quick Access Recorder(QAR)flight data with the wake vortex evolution model.Then,to consider the uniqueness of the medium-short-range turbofan aircraft,the severity of the wake vortex encounters was simulated using an induced roll moment coefficient.The proposed high-altitude wake vortex encounter model was able to identify and assess the highaltitude wake vortex changes,the bearing moments at different altitudes,and the atmospheric pressure conditions.Using the latest wake separation standards from the International Civil Aviation Organization(ICAO),acceptable safety wake intervals for follower aircraft in different scenarios were determined for the safety assessment.The results indicate that compared to mid and low altitudes,the high-altitude aircraft wake vortex dissipation rate is faster,the ultimate bearing moment is weaker,and the roll moment coefficient is higher,which confirm that there is elevated wake vortex encounter severity for regional turbofan aircraft.As safety is found to deteriorate when encountering wake vortices at altitudes higher than 8 km,new medium-short-range turbofan regional aircraft require higher safety margins than the latest wake separation standards.
基金supported by the AMS Funding Project(No.ZZB2023C7010).
文摘Glial fibrillary acidic protein(GFAP)is one of the discriminative biomarkers for diagnosing traumatic brain injury(TBI),and accurate determination of GFAP is clinically significant.In this study,a novel fluorescence immunoassay system was designed.We encapsulated carbon dots with a high fluorescence quantum yield(QY=92.5%)inside silicon nanocapsules to serve as fluorescent markers.These markers were then integrated with the streptavidin(SA)-biotin biomagnification system and immunomagnetic separation technology for the sensitive detection of GFAP.Based on the signal cascade amplification effect of the silicon nanocapsules and SA-biotin,the fluorescence signal of the SA-biotin-modified immunofluorescence nanocapsules increased 3.6-fold compared to the carbon dot-based immunoprobe.The fluorescence immunoassay system was constructed for GFAP using SA-biotin-modified immunocapsules as the sensing probe and immunomagnetic nanoparticles as the immunorecognition probe.The fluorescence immunoassay system can specifically and ultra-sensitively quantify GFAP in blood samples,with a detection range of 10 pg/mL–10 ng/mL and detection limits of 3.2 pg/mL(serum)and 3.6 pg/mL(plasma).Moreover,the fluorescence immunoassay system exhibited prominent recoveries of 99.4%–100.4%(phosphate buffered saline),96%–102.6%(serum),and 93.2%–110.2%(plasma),with favorable specificity and excellent stabilization.The novel fluorescence immunoassay system provides a new approach to the clinical analysis of GFAP and may serve as a potential tool for screening and diagnosing TBI.
基金supported by National Natural Science Foundation of China (Nos.52100032,51838005)Shandong Province Postdoctoral Program for Innovative Talent Support Plan (No.SDBX2022003)the Introduced Innovative R&D Team Project under the“The Pearl River Talent Recruitment Program”of Guangdong Province (2019ZT08L387).
文摘The adsorption of pollutants can not only promote the direct surface reaction,but also modify the catalyst itself to improve its photoelectric characteristics,which is rarely studied for water treatment with inorganic photocatalyst.A highly crystalline BiOBr(c-BiOBr)was synthesized by a two-step preparation process.Owing to the calcination,the highly crystalline enhanced the interface interaction between pollutant and c-BiOBr.The complex of organic pollutant and[Bi_(2)O_(2)]^(2+)could promote the active electron transfer from the adsorbed pollutant to c-BiOBr for the direct pollutant degradation by holes(h^(+)).Moreover,the pollutant adsorption actually modified c-BiOBr and promoted more unpaired electrons,which would coupling with the photoexcitation to promote generate more O_(2)^(•-).The molecular modification effect derived from pollutant adsorption significantly improved the removal of pollutants.This work strongly deepens the understanding of the molecularmodification effect from the pollutant adsorption and develops a novel and efficient approach for water treatment.
文摘Objective:To investigate the impact of programmed harmonious nursing combined with hierarchical management on nursing quality and satisfaction in a health management center.Methods:A total of 100 patients who received care at this health management center from January 2024 to January 2025 were selected as subjects.Using a random number table method,they were divided into an observation group(n=50)and a control group(n=50).The control group followed traditional methods,while the observation group integrated programmed harmonious nursing with hierarchical management.Comparative analysis was conducted on nursing quality scores,adverse event occurrence rates,and patient satisfaction between the two groups.Results:The observation group showed significantly improved nursing quality scores(P<0.05)and markedly reduced incidence of adverse events(P<0.05),with statistically significant differences compared to pre-treatment conditions(P<0.05).Conclusion:The combination of hierarchical management and programmed harmonious nursing demonstrates effectiveness in enhancing medical service quality,reducing adverse reactions,and improving patient satisfaction—a method worthy of promotion.
文摘AIM： To investigate the role of Livin and vascular endothelial growth factor （VEGF） in human esophageal carcinoma, and analyze its relationship to clinical stages.METHODS： Expression of Livin in fresh esophageal cancer tissues was detected by immunohistochemistry （IHC）, Western blotting and reverse transcriptasepolyrnerase chain reaction （RT-PCR）, and VEGF by Western blotting and RT-PCR. All statistical analyses were performed by SPSS version 11.0. RESULTS： Livin positivity was also significantly correlated with tumor stages, increasing with tumor progression. Expression of Livin and VEGF increased with the process of esophageal carcinoma. In the fourth clinical stage, expression of Livin and VEGF was the most significant. Expression of Livin was positively correlated with VEGF. CONCLUSION： Over-expression of Livin and VEGF contributes to the pathogenesis of esophageal carcinoma.
基金Supported by the National Natural Science Foundation of China,No.81270461,No.81570483 and No.81770541.
文摘BACKGROUND Although total or subtotal colectomy for slow-transit constipation(STC)has been proven to be a definite treatment,the associated defecation function and quality of life(QOL)are rarely studied.AIM To evaluate the effectiveness of surgery for STC regarding defecation function and QOL.METHODS From March 2013 to September 2017,30 patients undergoing surgery for STC in our department were analyzed.Preoperative,intra-operative,and postoperative 3-mo,6-mo,1-year,and 2-year follow-up details were recorded.Defecation function was assessed by bowel movements,abdominal pain,bloating,straining,laxative,enema use,diarrhea,and the Wexner constipation and incontinence scales.QOL was evaluated using the gastrointestinal QOL index and the 36-item short form survey.RESULTS The majority of patients(93.1%,27/29)stated that they benefited from the operation at the 2-year follow-up.At each time point of the follow-up,the number of bowel movements per week significantly increased compared with that of the preoperative conditions(P<0.05).Similarly,compared with the preoperative values,a marked decline was observed in bloating,straining,laxative,and enema use at each time point of the follow-up(P<0.05).Postoperative diarrhea could be controlled effectively and notably improved at the 2-year follow-up.The Wexner incontinence scores at 6-mo,1-year,and 2-year were notably lower than those at the 3-mo follow-up(P<0.05).Compared with those of the preoperative findings,the Wexner constipation scores significantly decreased following surgery(P<0.05).Thus,it was reasonable to find that the gastrointestinal QOL index scores clearly increase(P<0.05)and that the 36-item short form survey results displayed considerable improvements in six spheres(role physical,role emotional,physical pain,vitality,mental health,and general health)following surgery.CONCLUSION Total or subtotal colectomy for STC is not only effective in alleviating constipation-related symptoms but also in enhancing patients’QOL.
基金Natural Science Foundation of Shanghai of China,No.19ZR1441500.
文摘BACKGROUND It is important to differentiate benign and malignant focal liver lesions(FLLs)accurately.Despite the wide use and acceptance of shear wave elastography(SWE),its value for assessing the elasticity of FLLs and differentiating benign and malignant FLLs is still investigational.Previous studies of SWE for FLLs used mean elasticity as the parameter to reflect the stiffness of FLLs.Considering the inhomogeneity of tumor stiffness,maximal elasticity(Emax)might be the suitable parameter to reflect the stiffness of FLLs and to differentiate malignant FLLs from benign ones.AIM To explore the value of SWE with Emax in differential diagnosis of solid FLLs.METHODS We included 104 solid FLLs in 95 patients and 50 healthy volunteers.All the subjects were examined using conventional ultrasound(US)and virtual touch tissue quantification(VTQ)imaging.A diagnosis of benign or malignant FLL was made using conventional US.Ten VTQ values were acquired after 10 consecutive measurements for each FLL and each normal liver,and the largest value was recorded as Emax.RESULTS There were 56 cases of malignant FLLs and 48 cases of benign FLLs in this study.Emax of malignant FLLs(3.29±0.88 m/s)was significantly higher than that of benign FLLs(1.30±0.46 m/s,P<0.01)and that of livers in healthy volunteers(1.15±0.17 m/s,P<0.01).The cut-off point of Emax was 1.945,and the area under the curve was 0.978.The sensitivity and specificity of Emax were 92.9%and 91.7%,respectively,higher(but not significantly)than those of conventional US(80.4%for sensitivity and 81.3%for specificity).Combined diagnosis of conventional US and Emax using parallel testing improved the sensitivity to 100%with specificity of 75%.CONCLUSION SWE is a convenient and easy method to obtain accurate stiffness information of solid FLLs.Emax is useful for differential diagnosis of FLLs,especially in combination with conventional US.
文摘Objectives To investigate the overall prevalence of cardiovascular disease （CVD） in subjects hospitalized for chronic obstructive pulmonary disease （COPD）,and explore the prevalence of the major CVD complications and trends in patients with COPD over a 10-year period.Methods Medical records in the PLA General Hospital,Beijing Union Medical College Hospital,and Beijing Hospital from 2000/01/01 to 2010/03/03 were retrospectively reviewed.A total of 4960 patients with COPD were reviewed in the study （3570 males,mean age,72.2 ± 10.5 years; 1390 females,mean age,72.0 ± 10.4 years）.Results The prevalence of CVD in COPD patients was 51.7％.The three most prevalent CVDs were ischemic heart disease （28.9％）,heart failure （19.6％）,and arrhythmia （12.6％）.During the 10-year study period,the prevalence of various CVDs in COPD patients showed a gradual increasing trend with increasing age.There was higher morbidity due to ischemic heart disease （P ＜ 0.01） in male COPD patients than in the female counterparts.However,heart failure （P ＜ 0.01）and hypertension （P ＜ 0.01） occurred less frequently in male COPD patients than in female COPD patients.Furthermore,the prevalence of ischemic heart disease decreased year by year.In addition to heart failure,various types of CVD complications in COPD patients tended to occur in younger subjects.The prevalence of all major types of CVD in women tended to increase year by year.Conclusions The prevalence of CVD in patients hospitalized for COPD in Beijing was high.Age,sex and CVD trends,as well as life style changes,should be considered when prevention and control strategies are formulated.
基金This work was supported by State Grid Science and Technology Project(B3440821K003).
文摘The accurate fault-cause identification for overhead transmission lines supports the operation and maintenance personnel in formulating targeted maintenance strategies and shortening the time of inspecting faulty lines.With the goal of achieving“carbon peak and carbon neutrality”,the schemes for clean energy generation have rapidly developed.Moreover,new energy-consuming equipment has been widely connected to the power grid,and the operating characteristics of the power system have significantly changed.Consequently,these have impacted traditional fault identification methods.Based on the time-frequency characteristics of the fault waveform,new energy-related parameters,and deep learning model,this study proposes a fault identification method suitable for scenarios where a high proportion of new energy is connected to the power grid.Ten parameters related to the causes of transmission line fault and new energy connection scenarios are selected as model characteristic parameters.Further,a fault identification model based on adaptive deep belief networks was constructed,and its effect was verified by field data.
