AIM: To evaluate the sensitivity of brush cytology and forceps biopsy in a homogeneous patient group with hilar cholangiocarcinoma.METHODS: Brush cytology and forceps biopsy were routinely performed in patients with s...AIM: To evaluate the sensitivity of brush cytology and forceps biopsy in a homogeneous patient group with hilar cholangiocarcinoma.METHODS: Brush cytology and forceps biopsy were routinely performed in patients with suspected malignant biliary strictures. Fifty-eight consecutive patients undergoing endoscopic retrograde cholangio-pancreatography (ERCP) including forceps biopsy and brush cytology in patients with hilar cholangiocarcinoma between 1995-2005.RESULTS: Positive results for malignancy were obtained in 24/58 patients (41.4%) by brush cytology and in 31/58 patients (53.4%) by forceps biopsy. The combination of both techniques brush cytology and forceps biopsy resulted only in a minor increase in diagnostic sensitivity to 60.3% (35/58 patients). In 20/58 patients (34.5%), diagnosis were obtained by both positive cytology and positive histology, in 11/58 (19%) by positive histology (negative cytology) and only 4/58 patients (6.9%) were confirmed by positive cytology (negative histology).CONCLUSION: Brush cytology and forceps biopsy have only limited sensitivity for the diagnosis of malignant hilar tumors. In our eyes, additional diagnostic techniques should be evaluated and should become routine in patients with negative cytological and histological findings.展开更多
Taji et al . have reported in their study on 13 patients with gastric mucosa-associated lymphoid tissue （MALT） lymphomas an aggressive tumor course in trisomy 3 positive cases. The authors analyzed only stage I pat...Taji et al . have reported in their study on 13 patients with gastric mucosa-associated lymphoid tissue （MALT） lymphomas an aggressive tumor course in trisomy 3 positive cases. The authors analyzed only stage I patients with classical low-grade marginal zone lymphoma of the MALT type and detected the trisomy 3 using an alphasatellite DNA probe directed to the centromere. Their data support the observation that trisomy 3 is the most frequent cytogenetic aberration in MALT lymphomas .展开更多
Multispectral imaging is a common method to enhance tissue differentiation based on different cell or tissue structures.These differences arise from the unique structural properties of each tissue type,which result in...Multispectral imaging is a common method to enhance tissue differentiation based on different cell or tissue structures.These differences arise from the unique structural properties of each tissue type,which result in characteristic differences in spectral absorption.In the context of distinguishing between healthy and malignant tissues,differences are observed not only in spectral absorption but also in their elastic properties.To improve the reliability of measurement devices,we present a novel bimodal measurement system that includes a hyperspectral and an elastographic Fourier transform profilometry system to combine the individual results and to improve the overall task of tissue differentiation.The results of both subsystems show good ability to differentiate between healthy and malignant tissue.However,some limitations were identified for each individual sensor concept,such as contamination with pathological dye in hyperspectral imaging and insufficient sample thickness in elastographic measurements.Nevertheless,the multimodal combination of the individual sensor systems ensures good tissue differentiation.While the elastographic sensor can differentiate tissue even when contaminated with pathological staining agents,hyperspectral imaging can be used on very thin tissue samples and also enables the detection of tumor boundaries.展开更多
The incidence of metastatic disease in the central nervous system(CNS)is rising.According to current estimates,up to a third of adult cancer patients will suffer from CNS metastasis.Clinical evidence-based data from p...The incidence of metastatic disease in the central nervous system(CNS)is rising.According to current estimates,up to a third of adult cancer patients will suffer from CNS metastasis.Clinical evidence-based data from prospective randomized trials are rare,however,because CNS metastasis patients were often excluded from clinical trial participation.The management of CNS metastasis patients is therefore rather ill-defined and an interdisciplinary challenge.Recent basic and translational science data have begun contributing to a more profound understanding of the molecular mechanisms leading to invasion of tumor cells into the CNS.This report reviews advances,challenges,and perspectives in this field.展开更多
文摘AIM: To evaluate the sensitivity of brush cytology and forceps biopsy in a homogeneous patient group with hilar cholangiocarcinoma.METHODS: Brush cytology and forceps biopsy were routinely performed in patients with suspected malignant biliary strictures. Fifty-eight consecutive patients undergoing endoscopic retrograde cholangio-pancreatography (ERCP) including forceps biopsy and brush cytology in patients with hilar cholangiocarcinoma between 1995-2005.RESULTS: Positive results for malignancy were obtained in 24/58 patients (41.4%) by brush cytology and in 31/58 patients (53.4%) by forceps biopsy. The combination of both techniques brush cytology and forceps biopsy resulted only in a minor increase in diagnostic sensitivity to 60.3% (35/58 patients). In 20/58 patients (34.5%), diagnosis were obtained by both positive cytology and positive histology, in 11/58 (19%) by positive histology (negative cytology) and only 4/58 patients (6.9%) were confirmed by positive cytology (negative histology).CONCLUSION: Brush cytology and forceps biopsy have only limited sensitivity for the diagnosis of malignant hilar tumors. In our eyes, additional diagnostic techniques should be evaluated and should become routine in patients with negative cytological and histological findings.
文摘Taji et al . have reported in their study on 13 patients with gastric mucosa-associated lymphoid tissue （MALT） lymphomas an aggressive tumor course in trisomy 3 positive cases. The authors analyzed only stage I patients with classical low-grade marginal zone lymphoma of the MALT type and detected the trisomy 3 using an alphasatellite DNA probe directed to the centromere. Their data support the observation that trisomy 3 is the most frequent cytogenetic aberration in MALT lymphomas .
基金framework of the Graduate School 2543/2"Intraoperative Multi-Sensory Tissue Differentiation in Oncology"(Project ID 40947457,subprojects A1,A2,C2,and C3)funded by the German Research Foundation(DFG-Deutsche Forschungsgemeinschaft).
文摘Multispectral imaging is a common method to enhance tissue differentiation based on different cell or tissue structures.These differences arise from the unique structural properties of each tissue type,which result in characteristic differences in spectral absorption.In the context of distinguishing between healthy and malignant tissues,differences are observed not only in spectral absorption but also in their elastic properties.To improve the reliability of measurement devices,we present a novel bimodal measurement system that includes a hyperspectral and an elastographic Fourier transform profilometry system to combine the individual results and to improve the overall task of tissue differentiation.The results of both subsystems show good ability to differentiate between healthy and malignant tissue.However,some limitations were identified for each individual sensor concept,such as contamination with pathological dye in hyperspectral imaging and insufficient sample thickness in elastographic measurements.Nevertheless,the multimodal combination of the individual sensor systems ensures good tissue differentiation.While the elastographic sensor can differentiate tissue even when contaminated with pathological staining agents,hyperspectral imaging can be used on very thin tissue samples and also enables the detection of tumor boundaries.
基金Intramural Funding of the Center for Personalized Medicine(Demonstratorprojekt“CNSMet”).
文摘The incidence of metastatic disease in the central nervous system(CNS)is rising.According to current estimates,up to a third of adult cancer patients will suffer from CNS metastasis.Clinical evidence-based data from prospective randomized trials are rare,however,because CNS metastasis patients were often excluded from clinical trial participation.The management of CNS metastasis patients is therefore rather ill-defined and an interdisciplinary challenge.Recent basic and translational science data have begun contributing to a more profound understanding of the molecular mechanisms leading to invasion of tumor cells into the CNS.This report reviews advances,challenges,and perspectives in this field.
