Background and Objectives: Dengue is an arbovirosis caused by the dengue virus with 04 serotypes. The aim of the study was to characterise the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of the dengue virus cir...Background and Objectives: Dengue is an arbovirosis caused by the dengue virus with 04 serotypes. The aim of the study was to characterise the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of the dengue virus circulating in Ouagadougou, Burkina Faso. Methods: This was a descriptive analytical study that included 2833 patients and was carried out from January 2021 to December 2022. Rapid diagnosis of dengue was performed using the “Dengue Duo (AgNS1/IgM/IgG)” kit (SD Bioline, Korea). Viral RNA was extracted using the QIAGEN RNA RNeasy Plus Mini Kit (Quiagen, Germany) and virus serotypes were identified using the DENGUE Real-TM Genotype PCR kit (Sacace biotechnologies, Italy). Platelet counts were also performed using the XN-1500 Sysmex. Results: The prevalence of acute infections (NS1Ag positive) by TDR was 5.7% (162/2833), with the peak of dengue virus infection occurring between October and November. On the other hand, the AgNS1+ samples tested by RT-PCR were 53.7% positive for dengue virus;this shows the extent of probable cross-reactions with rapid diagnostic tests and false positives. Serotype 1 accounted for 52.6%, 28.4% had serotype 3, 16.8% had serotype 2 and 2.1% had serotype 4. We found cases of co-infection with DENV-1 and DENV-2 in two patients, co-infection with DENV-1 and DENV-3 in three patients, co-infection with DENV-1 and DENV-4 in one patient, co-infection with DENV-3 and DENV-4 in one patient and co-infection with three serotypes, DENV-1, DENV-2 and DENV-3 in one patient. Conclusion: The study showed that all four serotypes of the dengue virus were circulating in Ouagadougou. Serotype 1 was predominant.展开更多
Background:Dengue fever is a major public health problem in Burkina Faso.Some people infected by dengue virus develop a severe dengue(SD)and po-tentially fatal form of the disease which are dengue hemorrhagic syndrome...Background:Dengue fever is a major public health problem in Burkina Faso.Some people infected by dengue virus develop a severe dengue(SD)and po-tentially fatal form of the disease which are dengue hemorrhagic syndrome or dengue shock syndrome.Host genetic factors may be relevant,predisposing some individuals to severe disease.Polymorphisms of pro-inflammatory cy-tokines have been associated with the pathogenesis of dengue fever(DF).The aim of this study was to assess the involvement of IL10 cytokine gene poly-morphisms to restriction sequence(rs),rs1800872 and rs1800896 in the path-ogenesis of dengue fever and even in its progression to severe forms of the disease in Burkina Faso.We conducted a descriptive and analytical case-con-trol study.A total of 246 subjects were recruited including,with 117 people who had never been in contact with the dengue virus considered as controls and 129 dengue disease patients.Polymorphisms in the IL10-592 and IL10-1082 genes were obtained by restriction fragment length polymorphism(PCR-RFLP).We found that the AA genotype(46.81%/DF,53.57%/DS and 23.08%/control)of the IL10-592 polymorphism was a risk factor associated with the DS form and the CA heterozygote(21.31%)tended not to favor DF infection.The GG genotype of IL10-1082 was associated with dengue fever and possible progression from DF to DS,while the AG genotype was associ-ated with a higher risk of DF infection.The A allele of IL10-1082(OR=1.521 CI[1.046-2.215],p-value=0.027)was associated with the development of dengue and would not be indifferent in the progression to the DS form.Poly-morphisms in the IL10-1082 and IL10-592 genes seems to be associated with the pathogenesis of dengue disease and also with progression to the severe form of the disease.展开更多
文摘Background and Objectives: Dengue is an arbovirosis caused by the dengue virus with 04 serotypes. The aim of the study was to characterise the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) of the dengue virus circulating in Ouagadougou, Burkina Faso. Methods: This was a descriptive analytical study that included 2833 patients and was carried out from January 2021 to December 2022. Rapid diagnosis of dengue was performed using the “Dengue Duo (AgNS1/IgM/IgG)” kit (SD Bioline, Korea). Viral RNA was extracted using the QIAGEN RNA RNeasy Plus Mini Kit (Quiagen, Germany) and virus serotypes were identified using the DENGUE Real-TM Genotype PCR kit (Sacace biotechnologies, Italy). Platelet counts were also performed using the XN-1500 Sysmex. Results: The prevalence of acute infections (NS1Ag positive) by TDR was 5.7% (162/2833), with the peak of dengue virus infection occurring between October and November. On the other hand, the AgNS1+ samples tested by RT-PCR were 53.7% positive for dengue virus;this shows the extent of probable cross-reactions with rapid diagnostic tests and false positives. Serotype 1 accounted for 52.6%, 28.4% had serotype 3, 16.8% had serotype 2 and 2.1% had serotype 4. We found cases of co-infection with DENV-1 and DENV-2 in two patients, co-infection with DENV-1 and DENV-3 in three patients, co-infection with DENV-1 and DENV-4 in one patient, co-infection with DENV-3 and DENV-4 in one patient and co-infection with three serotypes, DENV-1, DENV-2 and DENV-3 in one patient. Conclusion: The study showed that all four serotypes of the dengue virus were circulating in Ouagadougou. Serotype 1 was predominant.
基金The World Academy of Sciences and the Swedish Inter-national Development Cooperation Agency(Sida)for funding this research through the grant No.17-403 RG/BIO/AF/AC_I-FR3240297757.
文摘Background:Dengue fever is a major public health problem in Burkina Faso.Some people infected by dengue virus develop a severe dengue(SD)and po-tentially fatal form of the disease which are dengue hemorrhagic syndrome or dengue shock syndrome.Host genetic factors may be relevant,predisposing some individuals to severe disease.Polymorphisms of pro-inflammatory cy-tokines have been associated with the pathogenesis of dengue fever(DF).The aim of this study was to assess the involvement of IL10 cytokine gene poly-morphisms to restriction sequence(rs),rs1800872 and rs1800896 in the path-ogenesis of dengue fever and even in its progression to severe forms of the disease in Burkina Faso.We conducted a descriptive and analytical case-con-trol study.A total of 246 subjects were recruited including,with 117 people who had never been in contact with the dengue virus considered as controls and 129 dengue disease patients.Polymorphisms in the IL10-592 and IL10-1082 genes were obtained by restriction fragment length polymorphism(PCR-RFLP).We found that the AA genotype(46.81%/DF,53.57%/DS and 23.08%/control)of the IL10-592 polymorphism was a risk factor associated with the DS form and the CA heterozygote(21.31%)tended not to favor DF infection.The GG genotype of IL10-1082 was associated with dengue fever and possible progression from DF to DS,while the AG genotype was associ-ated with a higher risk of DF infection.The A allele of IL10-1082(OR=1.521 CI[1.046-2.215],p-value=0.027)was associated with the development of dengue and would not be indifferent in the progression to the DS form.Poly-morphisms in the IL10-1082 and IL10-592 genes seems to be associated with the pathogenesis of dengue disease and also with progression to the severe form of the disease.
