Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α...Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis （TB） among patients with coal worker＇s pneumoconiosis （CWP）. Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism （PCR-RFLP）. Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher＇s exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls （2,2=5.44, P=-0.07）. But difference in frequency of the TNF-α-308 A allele was identified between them （2,2-5.14, P=0.02）. No significant difference in frequencies of the TNF-α-238 genotype and allele （P=0.23 and P=0.09, respectively） was found between cases and controls either, with combined （GG and AA） OR of 3.96 （95% confidence interval of 1.30-12.09） at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 （95% CI of 1.06-3.71） for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index （OR=4.92）, as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.展开更多
目的探讨肺泡动脉氧分压差[P_((A-a))O_2]对急性肺栓塞(APE)患者远期生存状况的影响。方法回顾性收集2011~2016年在唐山市3所三甲医院首次确诊为APE患者的病历资料,并电话随访收集患者的生存信息。根据P_((A-a))O_2与APE患者预后的ROC曲...目的探讨肺泡动脉氧分压差[P_((A-a))O_2]对急性肺栓塞(APE)患者远期生存状况的影响。方法回顾性收集2011~2016年在唐山市3所三甲医院首次确诊为APE患者的病历资料,并电话随访收集患者的生存信息。根据P_((A-a))O_2与APE患者预后的ROC曲线,将患者分成2组:P_((A-a))O_2<41.5 mm Hg组和P_((A-a))O_2≥41.5 mm Hg组。采用Kaplan-Meier法绘制生存曲线,采用COX比例风险回归模型分析P_((A-a))O_2水平与APE患者出院后死亡的关系。结果共432例APE患者进入分析,其中59例发生出院后死亡。P_((A-a))O_2<41.5 mm Hg组和P_((A-a))O_2≥41.5 mm Hg组的出院后病死率分别为2.7%和31.0%,调整其他影响因素后,P_((A-a))O_2≥41.5 mmHg可提高APE患者的出院死亡风险[H~^R=1.776(95%CI:1.006,3.136)]。结论P_((A-a))O_2水平可以预示APE患者的预后。展开更多
基金supported by grants from China National Programs for Science and Technology Development (Grant No. 2003BA712A11-24)Scientific Research Fund of North China Coal Medical College (Grant No. 2005-14)
文摘Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis （TB） among patients with coal worker＇s pneumoconiosis （CWP）. Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism （PCR-RFLP）. Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher＇s exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls （2,2=5.44, P=-0.07）. But difference in frequency of the TNF-α-308 A allele was identified between them （2,2-5.14, P=0.02）. No significant difference in frequencies of the TNF-α-238 genotype and allele （P=0.23 and P=0.09, respectively） was found between cases and controls either, with combined （GG and AA） OR of 3.96 （95% confidence interval of 1.30-12.09） at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 （95% CI of 1.06-3.71） for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index （OR=4.92）, as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.
文摘目的探讨肺泡动脉氧分压差[P_((A-a))O_2]对急性肺栓塞(APE)患者远期生存状况的影响。方法回顾性收集2011~2016年在唐山市3所三甲医院首次确诊为APE患者的病历资料,并电话随访收集患者的生存信息。根据P_((A-a))O_2与APE患者预后的ROC曲线,将患者分成2组:P_((A-a))O_2<41.5 mm Hg组和P_((A-a))O_2≥41.5 mm Hg组。采用Kaplan-Meier法绘制生存曲线,采用COX比例风险回归模型分析P_((A-a))O_2水平与APE患者出院后死亡的关系。结果共432例APE患者进入分析,其中59例发生出院后死亡。P_((A-a))O_2<41.5 mm Hg组和P_((A-a))O_2≥41.5 mm Hg组的出院后病死率分别为2.7%和31.0%,调整其他影响因素后,P_((A-a))O_2≥41.5 mmHg可提高APE患者的出院死亡风险[H~^R=1.776(95%CI:1.006,3.136)]。结论P_((A-a))O_2水平可以预示APE患者的预后。
