Dissolved organic carbon(DOC)and particulate organic carbon(POC)play essential roles in the carbon sequestration,with macroalgae being major producers of DOC and POC.The intertidal zone is the transition area between ...Dissolved organic carbon(DOC)and particulate organic carbon(POC)play essential roles in the carbon sequestration,with macroalgae being major producers of DOC and POC.The intertidal zone is the transition area between the ocean and the land,the main habitat of macroalgae.However,few studies have focused on the regulation of tidally induced desiccation-rewetting cycles on carbon sequestration by intertidal macroalgae.Therefore,we simulated the intertidal environments to investigate the effects of desiccation-rewetting cycles on the growth,DOC and POC release mechanisms of Ulva pertusa.After 14 days of experiments,the DOC release capacities of U.pertusa(per gram fresh weight)were 1.08,5.31,9.74 and 7.47 mg/g in the subtidal,low,middle and high tide zones,respectively.The corresponding POC release capacities were 0.04,1.00,3.90 and 1.38 mg/g.Combined biological carbon sequestration,the total carbon sequestration capacities of U.pertusa in the subtidal,low,middle and high tide zones were 24.73,32.84,27.83 and 16.97 mg/g,respectively.The results indicated that the highest carbon sequestration capacity of U.pertusa occurred in low tide zones.In conclusion,the results will provide support for the application of seaweed negative emissions.展开更多
Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-ba...Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.展开更多
基金the National Key Research and Development Program of China(No.2022YFC 3106001)the National Natural Science Foundation of China(No.42206131)+2 种基金the Projects of Science&Technology Plan in Yantai City(No.2023ZDCX039)the Ocean Negative Carbon Emissions(ONCE)Programthe Key Technology Research and Application of Ecological and Efficient Comprehensive Utilization in‘Fishery-Photovoltaic Integration’Seawater Ponds in the Yellow River Delta(No.DYYG2024-10)。
文摘Dissolved organic carbon(DOC)and particulate organic carbon(POC)play essential roles in the carbon sequestration,with macroalgae being major producers of DOC and POC.The intertidal zone is the transition area between the ocean and the land,the main habitat of macroalgae.However,few studies have focused on the regulation of tidally induced desiccation-rewetting cycles on carbon sequestration by intertidal macroalgae.Therefore,we simulated the intertidal environments to investigate the effects of desiccation-rewetting cycles on the growth,DOC and POC release mechanisms of Ulva pertusa.After 14 days of experiments,the DOC release capacities of U.pertusa(per gram fresh weight)were 1.08,5.31,9.74 and 7.47 mg/g in the subtidal,low,middle and high tide zones,respectively.The corresponding POC release capacities were 0.04,1.00,3.90 and 1.38 mg/g.Combined biological carbon sequestration,the total carbon sequestration capacities of U.pertusa in the subtidal,low,middle and high tide zones were 24.73,32.84,27.83 and 16.97 mg/g,respectively.The results indicated that the highest carbon sequestration capacity of U.pertusa occurred in low tide zones.In conclusion,the results will provide support for the application of seaweed negative emissions.
基金financial supports from Chinese Academy of Sciences"Innovation Fund"from Dalian Institute of Chemical Physics+7 种基金National Hi-Tech Research and Development Program of China(2006AA09Z435)the European Commission(Project:EVK3-CT1999-00005UVTOX)Ph.D.Research Fund from Science and Technology Bureau of Liaoning Province of China(20091019)Laboratory Program from Key Laboratory of Nearshore Marine Environmental Research of Liaoning Higher Education(LS2010024)Science and Technology Program from Ocean and Fisheries Department of Liaoning Province of China(200917)Open Laboratory Program from Key and Open Laboratory of Marine and Estuarine Fisheries Resources and EcologyMinistry of Agriculture of the People's Republic of China(Open-09-13)Public Welfare Projects from State Oceanic Administration(200805030)
文摘Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
