Paclitaxel is a widely used microtubule drug and cancer medicine.Here we report that by short exposure to paclitaxel at a low dose,multipolar spindles were induced in mitotic cells without centrosome amplification.Bot...Paclitaxel is a widely used microtubule drug and cancer medicine.Here we report that by short exposure to paclitaxel at a low dose,multipolar spindles were induced in mitotic cells without centrosome amplification.Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity.Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis.The surviving cells grew into clones with varied genome content.The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose,implicating potential side effects of paclitaxel.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.31071188,31030044,30721064,30900726 and 90913021)the National Key Basic Research and Development Program of China(Grant Nos.2010CB833705,2006CB910101 and 2007CB914502)
文摘Paclitaxel is a widely used microtubule drug and cancer medicine.Here we report that by short exposure to paclitaxel at a low dose,multipolar spindles were induced in mitotic cells without centrosome amplification.Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity.Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis.The surviving cells grew into clones with varied genome content.The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose,implicating potential side effects of paclitaxel.
