Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment.Herein,we developed a novel method to detect and evaluate biomarkers on cancer cell surface.Based on the exce...Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment.Herein,we developed a novel method to detect and evaluate biomarkers on cancer cell surface.Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells,we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction.The spectral characteristics of fluorophore naphthalimide has a good pH dependence.By this way,nucleolin-overexpressed cell could be discriminated from normal cell.Further,this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded(FFPE)tissue specimens.It is considered that our method has the potential to be applied in medical detection.展开更多
基金Supported by the National Program on Key Basic Research Project(973 Program)(2012CB720600,2012CB720603,2012CB720605)the National Natural Science Foundation of China(21432008,91413109,81373256,91213302)
文摘Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment.Herein,we developed a novel method to detect and evaluate biomarkers on cancer cell surface.Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells,we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction.The spectral characteristics of fluorophore naphthalimide has a good pH dependence.By this way,nucleolin-overexpressed cell could be discriminated from normal cell.Further,this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded(FFPE)tissue specimens.It is considered that our method has the potential to be applied in medical detection.
