中国气象局陆面数据同化系统(CLDAS)和高分辨率CLDAS(HRCLDAS)实况格点分析资料弥补了海面风观测稀疏的不足,然而其风速极大值与实际存在偏差,例如在狭管效应显著的台湾海峡区域普遍存在低估现象,其中CLDAS低估更严重,不能满足气象服务...中国气象局陆面数据同化系统(CLDAS)和高分辨率CLDAS(HRCLDAS)实况格点分析资料弥补了海面风观测稀疏的不足,然而其风速极大值与实际存在偏差,例如在狭管效应显著的台湾海峡区域普遍存在低估现象,其中CLDAS低估更严重,不能满足气象服务需求。因此本文提出一种基于地面站点观测风速二次融合的格点风场极大值订正技术,即采用反距离权重法将站点风速极大值观测周围一定影响半径区域内的站点风速观测插值到区域内的风场格点上,并综合考虑区域内风场误差和空间平滑程度以确定最优影响半径。利用2021~2023年CLDAS和HRCLDAS数据开展台湾海峡区域分区域(闽东、闽中及闽南渔场)风场极大值订正试验,结果表明:风场极大值订正技术能有效改进CLDAS和HRCLDAS对台湾海峡区域格点风速极大值低估情况,且HRCLDAS改进效果更优。HRCLDAS相较CLDAS出现小值最优影响半径频率增加。极值订正后,CLDAS和HRCLDAS逐小时平均绝对误差(MAE)降低率大多达到70%~85%和90%~95%;不同月份MAE降低率均超过60%,其中10月至次年1月订正效果最优,MAE降低率分别超过85%和90%;订正后误差空间分布与海岸线平行,自西向东减少,位于福建沿海及台湾海峡区域浮标站点区域MAE极值订正后降低至1 m s^(-1)以下。2305号台风“杜苏芮”和2023年1月23~25日典型冷空气大风过程的评估结果进一步表明,风场极大值订正技术对不同类别的大风过程均有效。展开更多
Ethylene response factors(ERFs)are plant transcription agents that play a pivotal role in disease resistance through the ethylene signaling pathway.However,whether and how ERFs regulate resistance to sheath blight(ShB...Ethylene response factors(ERFs)are plant transcription agents that play a pivotal role in disease resistance through the ethylene signaling pathway.However,whether and how ERFs regulate resistance to sheath blight(ShB),caused by Rhizoctonia solani in rice,remains largely unknown.Here,we demonstrated that OsERF7 negatively regulates rice resistance to ShB by inhibiting phytoalexin biosynthesis.Overexpression of OsERF7(OsERF7OE)significantly decreased ShB resistance,whereas knockout of OsERF7(oserf7)enhanced it.Mechanistically,antioxidant enzyme activities are significantly reduced in OsERF7OE plants,but increased in oserf7 plants.Furthermore,transcriptome analysis revealed that oserf7 plants exhibited significant upregulation of pathogenesis-related(PR)and phytoalexin biosynthesis genes upon R.solani infection.Consistently,transcript levels of phytoalexin biosynthesis genes,including OsKSL7,OsKSL8,OsKOL5,and OsCPS4,were significantly elevated in oserf7 plants,but reduced in OsERF7OE plants in response to R.solani infection.Electrophoretic mobility shift assays and dual-luciferase(LUC)reporter assays further confirmed that OsERF7 directly binds to the promoters of OsKSL8,OsKOL5,and OsCPS4,thereby repressing their expression.In summary,our study revealed that OsERF7 negatively regulated rice resistance to ShB primarily by inhibiting phytoalexin biosynthesis.展开更多
文摘中国气象局陆面数据同化系统(CLDAS)和高分辨率CLDAS(HRCLDAS)实况格点分析资料弥补了海面风观测稀疏的不足,然而其风速极大值与实际存在偏差,例如在狭管效应显著的台湾海峡区域普遍存在低估现象,其中CLDAS低估更严重,不能满足气象服务需求。因此本文提出一种基于地面站点观测风速二次融合的格点风场极大值订正技术,即采用反距离权重法将站点风速极大值观测周围一定影响半径区域内的站点风速观测插值到区域内的风场格点上,并综合考虑区域内风场误差和空间平滑程度以确定最优影响半径。利用2021~2023年CLDAS和HRCLDAS数据开展台湾海峡区域分区域(闽东、闽中及闽南渔场)风场极大值订正试验,结果表明:风场极大值订正技术能有效改进CLDAS和HRCLDAS对台湾海峡区域格点风速极大值低估情况,且HRCLDAS改进效果更优。HRCLDAS相较CLDAS出现小值最优影响半径频率增加。极值订正后,CLDAS和HRCLDAS逐小时平均绝对误差(MAE)降低率大多达到70%~85%和90%~95%;不同月份MAE降低率均超过60%,其中10月至次年1月订正效果最优,MAE降低率分别超过85%和90%;订正后误差空间分布与海岸线平行,自西向东减少,位于福建沿海及台湾海峡区域浮标站点区域MAE极值订正后降低至1 m s^(-1)以下。2305号台风“杜苏芮”和2023年1月23~25日典型冷空气大风过程的评估结果进一步表明,风场极大值订正技术对不同类别的大风过程均有效。
基金supported by the National Natural Science Foundation of China(Grant Nos.32200430 and 32272110)Jiangsu Province Higher Vocational College Teacher Professional Leader High-End Training Program,China(Grant No.2023GRFX083)+2 种基金China Postdoctoral Science Foundation(Grant No.2022M712700)Jiangsu Funding Program for Excellent Postdoctoral Talent,China(Grant No.2022ZB628)a Project Funded by Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘Ethylene response factors(ERFs)are plant transcription agents that play a pivotal role in disease resistance through the ethylene signaling pathway.However,whether and how ERFs regulate resistance to sheath blight(ShB),caused by Rhizoctonia solani in rice,remains largely unknown.Here,we demonstrated that OsERF7 negatively regulates rice resistance to ShB by inhibiting phytoalexin biosynthesis.Overexpression of OsERF7(OsERF7OE)significantly decreased ShB resistance,whereas knockout of OsERF7(oserf7)enhanced it.Mechanistically,antioxidant enzyme activities are significantly reduced in OsERF7OE plants,but increased in oserf7 plants.Furthermore,transcriptome analysis revealed that oserf7 plants exhibited significant upregulation of pathogenesis-related(PR)and phytoalexin biosynthesis genes upon R.solani infection.Consistently,transcript levels of phytoalexin biosynthesis genes,including OsKSL7,OsKSL8,OsKOL5,and OsCPS4,were significantly elevated in oserf7 plants,but reduced in OsERF7OE plants in response to R.solani infection.Electrophoretic mobility shift assays and dual-luciferase(LUC)reporter assays further confirmed that OsERF7 directly binds to the promoters of OsKSL8,OsKOL5,and OsCPS4,thereby repressing their expression.In summary,our study revealed that OsERF7 negatively regulated rice resistance to ShB primarily by inhibiting phytoalexin biosynthesis.
