目的旨在探讨老年人不同健康状况变化轨迹与认知障碍之间的关联,为认知障碍的早期筛查及预防提供科学依据。方法利用中国健康与养老追踪调查(China Health and Retirement Longitudinal Study,CHARLS)的3轮全国数据(2011—2015年),纳入...目的旨在探讨老年人不同健康状况变化轨迹与认知障碍之间的关联,为认知障碍的早期筛查及预防提供科学依据。方法利用中国健康与养老追踪调查(China Health and Retirement Longitudinal Study,CHARLS)的3轮全国数据(2011—2015年),纳入符合标准的3185名≥60岁成年人。通过潜类别轨迹模型识别认知功能得分和不同健康状况[握力、睡眠时长、基本日常生活活动(basic activities of daily living,BADLs)限制、抑郁症状]之间的纵向变化,采用Cox比例风险回归模型分析不同健康状况轨迹与认知障碍发生风险的关联,采用线性混合效应模型评估不同健康状况变化轨迹与认知功能得分之间的纵向关联。结果横断面分析显示,认知障碍者在基线时表现出低握力、短睡眠时长、BADLs限制和抑郁症状。在纵向队列研究中,Cox比例风险回归模型分析显示,抑郁症状高轨迹(HR=1.60,95%CI:1.31~1.96)、BADLs高限制轨迹(HR=1.39,95%CI:1.21~1.60)及短睡眠时长轨迹(HR=1.30,95%CI:1.13~1.49)增加认知障碍发生的风险,而握力高水平轨迹(HR=0.75,95%CI:0.63~0.90)可降低该风险。结论握力、睡眠时长、BADLs限制及抑郁症状的纵向变化轨迹可作为老年人认知障碍风险的早期识别指标,为针对性干预提供科学依据。展开更多
OBJECTIVE:To explore the mechanism of Tangfukang formula(糖复康方,TFK)in treating type 2 diabetes mellitus(T2DM).METHODS:We employed network pharmacology combined with experimental validation to explore the potential ...OBJECTIVE:To explore the mechanism of Tangfukang formula(糖复康方,TFK)in treating type 2 diabetes mellitus(T2DM).METHODS:We employed network pharmacology combined with experimental validation to explore the potential mechanism of TFK against T2DM.Initially,we filtered bioactive compounds with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and Symptom Mapping(Sym Map),and gathered targets of TFK and T2DM.Subsequently,we constructed a protein-protein interaction(PPI)network,enriched core targets through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and adopted molecular docking to study the binding mode of compounds and the signaling pathway.Finally,we employed a KKAy mice model to investigate the effect and mechanism of TFK against T2DM.Biochemical assay,histology assay,and Western blot(WB)were used to assess the mechanism.RESULTS:There were 492 bioactive compounds of TFK screened,and 1226 overlapping targets of TFK against T2DM identified.A compound-T2DM-related target network with 997 nodes and 4439 edges was constructed.KEGG enrichment analysis identified some core pathways related to T2DM,including adenosine 5-monophosphate-activated protein kinase(AMPK)signaling pathway.Molecular docking study revealed that compounds of TFK,including citric acid,could bind to the active pocket of AMPK crystal structure with free binding energy of-4.8,-8 and-7.9,respectively.Animal experiments indicated that TFK decreased body weight,fasting blood glucose,fasting serum insulin,homeostasis model of insulin resistance,glycosylated serum protein,total cholesterol,triglyceride,and low-density lipoprotein cholesterol,and improve oral glucose tolerance test results.TFK reduced steatosis in liver tissue,and infiltration of inflammatory cells,and protected liver cells to a certain extent.WB analysis revealed that,TFK upregulated the phosphorylation of AMPK and branchedchainα-ketoacid dehydrogenase proteins.CONCLUSION:TFK has the potential to effectively manage T2DM,possibly by regulating the AMPK signaling pathway.The present study lays a new foundation for the therapeutic application of TFK in the treatment of T2DM.展开更多
OBJECTIVE: To investigate the effect of a modified Banxia Xiexin decoction(MBXD) plus chemotherapy on postoperative adverse reaction in patients with stage Ⅲ colon cancer in patients whose symptoms were identified as...OBJECTIVE: To investigate the effect of a modified Banxia Xiexin decoction(MBXD) plus chemotherapy on postoperative adverse reaction in patients with stage Ⅲ colon cancer in patients whose symptoms were identified as cold-heat complicated pattern in terms of the theory of Traditional Chinese Medicine(TCM).METHODS: A prospective non-randomized control study of patients with stage Ⅲ colon cancer in Beijing Shijitan Hospital and Guang'anmen Hospital between January 2012 and December 2013. A total of 80 patients were divided into experimental group(MBXD + chemotherapy) and control group(chemotherapy). The adverse reactions, life quality and disease-free survival(DFS) were compared between the two groups.RESULTS: The cold-heat complex pattern was released in 94.3% of patients in experimental group whereas 62.2% in control group(P < 0.05). Life quality was improved in 24 vs 14 cases, stabilized in 9 vs14 cases whereas decreased in 2 vs 15 cases in experimental group and control group, respectively(P < 0.05). The average DFS was 22.371 months in experimental group, while 13.932 months in control group(P < 0.05).CONCLUSION: MBXD combined with chemotherapy may significantly relieve clinical symptoms, reduce chemotherapy associated adverse effects, improve life quality, and prolong DFS of patients with stage Ⅲ colon cancer(cold-heat complicated pattern).展开更多
文摘目的旨在探讨老年人不同健康状况变化轨迹与认知障碍之间的关联,为认知障碍的早期筛查及预防提供科学依据。方法利用中国健康与养老追踪调查(China Health and Retirement Longitudinal Study,CHARLS)的3轮全国数据(2011—2015年),纳入符合标准的3185名≥60岁成年人。通过潜类别轨迹模型识别认知功能得分和不同健康状况[握力、睡眠时长、基本日常生活活动(basic activities of daily living,BADLs)限制、抑郁症状]之间的纵向变化,采用Cox比例风险回归模型分析不同健康状况轨迹与认知障碍发生风险的关联,采用线性混合效应模型评估不同健康状况变化轨迹与认知功能得分之间的纵向关联。结果横断面分析显示,认知障碍者在基线时表现出低握力、短睡眠时长、BADLs限制和抑郁症状。在纵向队列研究中,Cox比例风险回归模型分析显示,抑郁症状高轨迹(HR=1.60,95%CI:1.31~1.96)、BADLs高限制轨迹(HR=1.39,95%CI:1.21~1.60)及短睡眠时长轨迹(HR=1.30,95%CI:1.13~1.49)增加认知障碍发生的风险,而握力高水平轨迹(HR=0.75,95%CI:0.63~0.90)可降低该风险。结论握力、睡眠时长、BADLs限制及抑郁症状的纵向变化轨迹可作为老年人认知障碍风险的早期识别指标,为针对性干预提供科学依据。
基金Tsinghua Precision Medicine Foundation:Tangfukang Plays the Therapeutic Role in Type 2 Diabetes Patients with Qi and Yin Deficiency Syndrome by Regulating the Intestinal Flora Mediated Branched-chain Amino Acids-Phosphatidylinositide 3-Kinases-Protein Kinase B Signaling Pathway(grant number 10001020105)National Natural Science Foundation of China:Tangfukang Plays the Therapeutic Role in Type 2 Diabetes Mellitus by Regulating the Intestinal Flora Mediated Adiponectin-adenosine 5-Monophosphate-activated Protein Kinase-branched-chain Amino Acids Signaling Pathway(grant number 82104812)。
文摘OBJECTIVE:To explore the mechanism of Tangfukang formula(糖复康方,TFK)in treating type 2 diabetes mellitus(T2DM).METHODS:We employed network pharmacology combined with experimental validation to explore the potential mechanism of TFK against T2DM.Initially,we filtered bioactive compounds with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and Symptom Mapping(Sym Map),and gathered targets of TFK and T2DM.Subsequently,we constructed a protein-protein interaction(PPI)network,enriched core targets through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and adopted molecular docking to study the binding mode of compounds and the signaling pathway.Finally,we employed a KKAy mice model to investigate the effect and mechanism of TFK against T2DM.Biochemical assay,histology assay,and Western blot(WB)were used to assess the mechanism.RESULTS:There were 492 bioactive compounds of TFK screened,and 1226 overlapping targets of TFK against T2DM identified.A compound-T2DM-related target network with 997 nodes and 4439 edges was constructed.KEGG enrichment analysis identified some core pathways related to T2DM,including adenosine 5-monophosphate-activated protein kinase(AMPK)signaling pathway.Molecular docking study revealed that compounds of TFK,including citric acid,could bind to the active pocket of AMPK crystal structure with free binding energy of-4.8,-8 and-7.9,respectively.Animal experiments indicated that TFK decreased body weight,fasting blood glucose,fasting serum insulin,homeostasis model of insulin resistance,glycosylated serum protein,total cholesterol,triglyceride,and low-density lipoprotein cholesterol,and improve oral glucose tolerance test results.TFK reduced steatosis in liver tissue,and infiltration of inflammatory cells,and protected liver cells to a certain extent.WB analysis revealed that,TFK upregulated the phosphorylation of AMPK and branchedchainα-ketoacid dehydrogenase proteins.CONCLUSION:TFK has the potential to effectively manage T2DM,possibly by regulating the AMPK signaling pathway.The present study lays a new foundation for the therapeutic application of TFK in the treatment of T2DM.
基金Supported by the Construction Program of Key Discipline of Combined Traditonal Chinese Medicine and Western Medicine for Neuroendocrine-Immunoregulatory Diseases,China [Beijing Regions Registration Number(2010)126]Construction Program of Beijing Institute of Combined TCM and Western Medicine for Cancers,China [Beijing Chinese Medicine Administration Registration Number(2015)154]
文摘OBJECTIVE: To investigate the effect of a modified Banxia Xiexin decoction(MBXD) plus chemotherapy on postoperative adverse reaction in patients with stage Ⅲ colon cancer in patients whose symptoms were identified as cold-heat complicated pattern in terms of the theory of Traditional Chinese Medicine(TCM).METHODS: A prospective non-randomized control study of patients with stage Ⅲ colon cancer in Beijing Shijitan Hospital and Guang'anmen Hospital between January 2012 and December 2013. A total of 80 patients were divided into experimental group(MBXD + chemotherapy) and control group(chemotherapy). The adverse reactions, life quality and disease-free survival(DFS) were compared between the two groups.RESULTS: The cold-heat complex pattern was released in 94.3% of patients in experimental group whereas 62.2% in control group(P < 0.05). Life quality was improved in 24 vs 14 cases, stabilized in 9 vs14 cases whereas decreased in 2 vs 15 cases in experimental group and control group, respectively(P < 0.05). The average DFS was 22.371 months in experimental group, while 13.932 months in control group(P < 0.05).CONCLUSION: MBXD combined with chemotherapy may significantly relieve clinical symptoms, reduce chemotherapy associated adverse effects, improve life quality, and prolong DFS of patients with stage Ⅲ colon cancer(cold-heat complicated pattern).
