Peroxiredoxins（Prxs） are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species（ROS）. Although extensive efforts have been made over th...Peroxiredoxins（Prxs） are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species（ROS）. Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye（Leymus Chinensis）, designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H2O2 and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na2CO3 in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na2CO3.展开更多
基金Supported by the Transgenic Plant Research Special Program of China(No.2008ZX08003-005)the Technology Development Project of Jilin Province,China(Nos.20086029,20076016)
文摘Peroxiredoxins（Prxs） are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species（ROS）. Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye（Leymus Chinensis）, designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H2O2 and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na2CO3 in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na2CO3.
