大型溞(Daphnia magna)急性活动抑制试验和繁殖试验是研究和评价新化学物质对水生生物危害性的重要手段之一。由于经济合作与发展组织(Organization for Economic Co-operation and Development,OECD)测试导则推荐的Elendt M4培养基中...大型溞(Daphnia magna)急性活动抑制试验和繁殖试验是研究和评价新化学物质对水生生物危害性的重要手段之一。由于经济合作与发展组织(Organization for Economic Co-operation and Development,OECD)测试导则推荐的Elendt M4培养基中含有的乙二胺四乙酸(EDTA)可能会影响金属离子的活性,不适合用于含有金属离子样品的毒理学测试。为选出合适的替代培养基,采用Elendt M4培养基、美国材料与试验协会(American Society for Testing and Materials,ASTM)推荐的ASTM培养基和国际标准化组织(International Organization for Standardization,ISO)推荐的ISO培养基进行大型溞繁殖试验,并收集繁殖试验中亲溞产下的非头胎幼溞进行重铬酸钾的急性活动抑制试验。结果表明,3种培养基对大型溞的生长、存活均无抑制作用,ASTM培养基和ISO培养基中大型溞的繁殖量分别为111±10和70±13,显著低于Elendt M4培养基的132±4(P<0.05)。3种培养基中大型溞的繁殖量均能满足OECD 211-2012大型溞繁殖试验标准中繁殖量大于60的要求,但在ISO培养基中有死胎出现。重铬酸钾对Elendt M4培养基、ASTM培养基和ISO培养基中繁殖幼溞的24 h半数效应浓度(24 h-EC50)值分别为1.29、1.84和0.772 mg·L-1,均在OECD 202-2004大型溞急性活动抑制试验标准要求的0.6~2.1 mg·L-1范围内,说明3种培养基中繁殖的大型溞均能用于大型溞急性活动抑制试验的标准测试中。综上所述,进行含有金属离子样品的毒理测试时,ASTM培养基和ISO培养基均可用于大型溞的短期驯养和急性活动抑制试验,ASTM培养基还可以用于长期驯养和繁殖试验。以上结果为大型溞毒性试验中试验培养基的选择提供理论依据。展开更多
Mesoscale eddies （MEs） in the South China Sea （SCS） simulated by a quasi-global eddy-resolving ocean general circulation model are evaluated against satellite data during 1993-2007. The modeled ocean data show mor...Mesoscale eddies （MEs） in the South China Sea （SCS） simulated by a quasi-global eddy-resolving ocean general circulation model are evaluated against satellite data during 1993-2007. The modeled ocean data show more activity than shown by the satellite data and reproduces more eddies in the SCS. A total of 345 （428） cyclonic eddies （CEs） and 330 （371） anti-cyclonic eddies （AEs） generated for satellite （model） data are identified during the study period, showing increase of -24% and -12% for the model data, respectively. Compared with eddies in satellite, the simulated eddies tend to have smaller radii, larger amplitudes, a slightly longer lifetime, faster movement and rotation speed, a slightly larger nonlinear properties （U/c） in the model. However, the spatial distribution of generated eddies appears to be inhomogeneous, with more CEs in the northern part of SCS and fewer AEs in the southern part. This is attributed to the exaggerated Kuroshio intrusion in the model because the small islands in the Luzon Strait are still not well resolved although the horizontal resolution reaches （1/10）°. The seasonal variability in the number and the amplitude of eddies generated is also investigated.展开更多
文摘大型溞(Daphnia magna)急性活动抑制试验和繁殖试验是研究和评价新化学物质对水生生物危害性的重要手段之一。由于经济合作与发展组织(Organization for Economic Co-operation and Development,OECD)测试导则推荐的Elendt M4培养基中含有的乙二胺四乙酸(EDTA)可能会影响金属离子的活性,不适合用于含有金属离子样品的毒理学测试。为选出合适的替代培养基,采用Elendt M4培养基、美国材料与试验协会(American Society for Testing and Materials,ASTM)推荐的ASTM培养基和国际标准化组织(International Organization for Standardization,ISO)推荐的ISO培养基进行大型溞繁殖试验,并收集繁殖试验中亲溞产下的非头胎幼溞进行重铬酸钾的急性活动抑制试验。结果表明,3种培养基对大型溞的生长、存活均无抑制作用,ASTM培养基和ISO培养基中大型溞的繁殖量分别为111±10和70±13,显著低于Elendt M4培养基的132±4(P<0.05)。3种培养基中大型溞的繁殖量均能满足OECD 211-2012大型溞繁殖试验标准中繁殖量大于60的要求,但在ISO培养基中有死胎出现。重铬酸钾对Elendt M4培养基、ASTM培养基和ISO培养基中繁殖幼溞的24 h半数效应浓度(24 h-EC50)值分别为1.29、1.84和0.772 mg·L-1,均在OECD 202-2004大型溞急性活动抑制试验标准要求的0.6~2.1 mg·L-1范围内,说明3种培养基中繁殖的大型溞均能用于大型溞急性活动抑制试验的标准测试中。综上所述,进行含有金属离子样品的毒理测试时,ASTM培养基和ISO培养基均可用于大型溞的短期驯养和急性活动抑制试验,ASTM培养基还可以用于长期驯养和繁殖试验。以上结果为大型溞毒性试验中试验培养基的选择提供理论依据。
基金The National Key Program for Developing Basic Science of China under contract No.2013CB956204the National Natural Science Foundation of China under contract Nos 41275084 and 41575084the Strategic Priority Research of the Chinese Academy of Science under contract No.XDA 11010304
文摘Mesoscale eddies （MEs） in the South China Sea （SCS） simulated by a quasi-global eddy-resolving ocean general circulation model are evaluated against satellite data during 1993-2007. The modeled ocean data show more activity than shown by the satellite data and reproduces more eddies in the SCS. A total of 345 （428） cyclonic eddies （CEs） and 330 （371） anti-cyclonic eddies （AEs） generated for satellite （model） data are identified during the study period, showing increase of -24% and -12% for the model data, respectively. Compared with eddies in satellite, the simulated eddies tend to have smaller radii, larger amplitudes, a slightly longer lifetime, faster movement and rotation speed, a slightly larger nonlinear properties （U/c） in the model. However, the spatial distribution of generated eddies appears to be inhomogeneous, with more CEs in the northern part of SCS and fewer AEs in the southern part. This is attributed to the exaggerated Kuroshio intrusion in the model because the small islands in the Luzon Strait are still not well resolved although the horizontal resolution reaches （1/10）°. The seasonal variability in the number and the amplitude of eddies generated is also investigated.
