Rice heading date,or flowering time,is important for yield optimization and regional adaptation.While many genes controlling this trait in rice are known,breeders often need only slight adjustments.Our study used the ...Rice heading date,or flowering time,is important for yield optimization and regional adaptation.While many genes controlling this trait in rice are known,breeders often need only slight adjustments.Our study used the CRISPR/Cas9 method to edit the Hd6 gene in the early-maturing,late-season japonica rice cultivar Nanjing 46(NJ46),creating three mutants with reduced flowering times of 9-12 d under natural conditions in Nanjing city,Jiangsu Province,China.These mutants showed higher Hd3a and RFT1 expression levels without compromising yield or eating and cooking quality,demonstrating that Hd6 gene editing is an effective precision breeding tool for shortening heading date without laborious traditional methods.展开更多
基金supported by the Ministry of Agriculture and Rural Affairs of China,the Jiangsu Provincial Key Research and Development Program (Grant No.BE2022383)the Natural Science Foundation of Jiangsu Province,China (Grant No.BK20212010)+2 种基金the STI 2030-Major Projects (Grant No.2023ZD04066)Jiangsu Agricultural Science and Technology Innovation Fund,China (Grant No.CX (22)3138)Zhongshan Biological Breeding Laboratory,China (Grant No.ZSBBL-KY2023-04-1)。
文摘Rice heading date,or flowering time,is important for yield optimization and regional adaptation.While many genes controlling this trait in rice are known,breeders often need only slight adjustments.Our study used the CRISPR/Cas9 method to edit the Hd6 gene in the early-maturing,late-season japonica rice cultivar Nanjing 46(NJ46),creating three mutants with reduced flowering times of 9-12 d under natural conditions in Nanjing city,Jiangsu Province,China.These mutants showed higher Hd3a and RFT1 expression levels without compromising yield or eating and cooking quality,demonstrating that Hd6 gene editing is an effective precision breeding tool for shortening heading date without laborious traditional methods.
