A Microcantilevers resonator is used to detect a protein biomarker called prostate specific antigen(PSA),which is associated with prostate cancer.Different concentrations of PSA in a buffer solution are detected as a ...A Microcantilevers resonator is used to detect a protein biomarker called prostate specific antigen(PSA),which is associated with prostate cancer.Different concentrations of PSA in a buffer solution are detected as a function of deflection of the beams.For this purpose,we use a surface micromachined,antibody-coated polycrystalline silicon micromechanical cantilever beam.Cantilevers have mass sensitivities of the order of 10–17 g/Hz,which result from their small mass.This matter allows them to detect an immobilized antibody monolayer corresponding to a mass of about 70 fg.With these devices,concentrations as low as 150 fg/mL,or 4.5 fM,could be detected from the realistic samples.展开更多
We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range o...We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. The experirnental results strongly indicate that the protein based LSPR chip can be successfully used as a simple, fast, sensitive and quantitative sensor for H2S detection.展开更多
文摘A Microcantilevers resonator is used to detect a protein biomarker called prostate specific antigen(PSA),which is associated with prostate cancer.Different concentrations of PSA in a buffer solution are detected as a function of deflection of the beams.For this purpose,we use a surface micromachined,antibody-coated polycrystalline silicon micromechanical cantilever beam.Cantilevers have mass sensitivities of the order of 10–17 g/Hz,which result from their small mass.This matter allows them to detect an immobilized antibody monolayer corresponding to a mass of about 70 fg.With these devices,concentrations as low as 150 fg/mL,or 4.5 fM,could be detected from the realistic samples.
文摘We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. The experirnental results strongly indicate that the protein based LSPR chip can be successfully used as a simple, fast, sensitive and quantitative sensor for H2S detection.
