In this work,in situ luminescence analysis was applied for the first time for monitoring the phase transitions of calcium phosphate(CaP)and confirmed by synchrotron in situ X-ray diffraction in addition to in situ inf...In this work,in situ luminescence analysis was applied for the first time for monitoring the phase transitions of calcium phosphate(CaP)and confirmed by synchrotron in situ X-ray diffraction in addition to in situ infrared spectroscopy,with simultaneous measurements of pH and ion conductivity.Applying doped Ce^(3+)and Eu^(3+)as local coordination sensors,the high sensitivity of their emission spectra upon the changes in the coordination sphere of the doped cation sites enabled to detect the formation of amorphous calcium phosphate(ACP)and Ca_(5)(PO_(4))_(3)OH,besides their subsequent transitions to CaHPO_(4)·2H_(2)O and Ca_(8)H_(2)(PO_(4))_(6)·5H_(2)O under real reaction conditions.Calcium phosphates are widely present in mammals and understanding their phase transitions is important to comprehend the conversion between healthy and diseased tissues.In situ luminescence measurements are advantageous for allowing monitoring these phase transitions in a fast and sensitive fashion also in conventional laboratories,independent of synchrotron radiation.展开更多
基金the development of the in situ analysis software as well as the DFG(Priority Program 1415,project BE 1653/29-1,project TE 1147/1-1),Daimler and Benz Foundation and MATsynCELL for the financial support.
文摘In this work,in situ luminescence analysis was applied for the first time for monitoring the phase transitions of calcium phosphate(CaP)and confirmed by synchrotron in situ X-ray diffraction in addition to in situ infrared spectroscopy,with simultaneous measurements of pH and ion conductivity.Applying doped Ce^(3+)and Eu^(3+)as local coordination sensors,the high sensitivity of their emission spectra upon the changes in the coordination sphere of the doped cation sites enabled to detect the formation of amorphous calcium phosphate(ACP)and Ca_(5)(PO_(4))_(3)OH,besides their subsequent transitions to CaHPO_(4)·2H_(2)O and Ca_(8)H_(2)(PO_(4))_(6)·5H_(2)O under real reaction conditions.Calcium phosphates are widely present in mammals and understanding their phase transitions is important to comprehend the conversion between healthy and diseased tissues.In situ luminescence measurements are advantageous for allowing monitoring these phase transitions in a fast and sensitive fashion also in conventional laboratories,independent of synchrotron radiation.
