Infections by Carbapenem-resistant Enterobacteriaceae(CRE)producing New Delhi metallo-β-lactamases(NDMs)that hydrolyze carbapenems,are difficult to treat.Considering the fact of barren antibiotic development pipeline...Infections by Carbapenem-resistant Enterobacteriaceae(CRE)producing New Delhi metallo-β-lactamases(NDMs)that hydrolyze carbapenems,are difficult to treat.Considering the fact of barren antibiotic development pipeline nowadays,revitalizing carbapenems efficacy using antibiotic adjuvants represents a promising but as-yet-unrealized tactic in combating NDMs-producing CRE,because of their off-target distributions,weak permeability to the outer membrane(OM)barrier,and limited NDMs-inactivation ability.Here,a pathogen-anchoring nano electrosensitizer,is proposed for serving as electrodynamic therapy(EDT)-activatable antibiotic adjuvant to revitalize meropenem(MEM,a typical carbapenems),through layerby-layer assembly of platinum nanoclusters(PtNCs),MEM,and maltodextrin(MA).The MA corona of electro-driven nanosensitizer promotes the efficient anchoring of PtNCs and MEM onto the surface of pathogen.Triggered by a square-wave alternating current(AC),MEM is released,PtNCs-based EDT is activated,accompanied by the generation of electro-driven reactive oxygen(ROS)at the bacterial infectious microenvironment.Subsequently,the antibacterial efficacy of MEM is thereby restored against NDMs-producing CRE through the ROS-mediated cascading effect,including the OM destruction,MEM intracellular accumulation,and NDMs inactivation.By the cooperation of MA-mediated pathogen anchoring and EDT-induced dual action,the pathogen-anchoring nano electrosensitizer revitalizes MEM efficacy against clinically isolated NDMs-producing CRE in vitro,and in mice infection model,importantly,also slows down the development of higher-level resistance in NDMsproducing CRE.The pathogen-anchoring nano electrosensitizer can fully play the therapeutic potency of carbapenems,providing a promising approach to tackle NDMs producer-induced infections.展开更多
This paper gives an overview of the distribution,member scale,production and operation of banana cooperatives in Hainan Province,and points out the market risk and natural risk faced by the production of banana cooper...This paper gives an overview of the distribution,member scale,production and operation of banana cooperatives in Hainan Province,and points out the market risk and natural risk faced by the production of banana cooperatives in Hainan Province. In order to promote the banana cooperatives to form new agricultural management system integrating organization and intensification,this paper puts forth the production and operation recommendations,such as joint production of banana cooperatives,timely planting of banana,brand management,and improvement of production and operation technical level.展开更多
The paper introduces the present development of farmers' specialized cooperatives in China, points out that although the cooperatives in China develop rapidly, they are generally small and their advantages cannot ...The paper introduces the present development of farmers' specialized cooperatives in China, points out that although the cooperatives in China develop rapidly, they are generally small and their advantages cannot be given into full play, and then analyzes the development achievements of the cooperatives, for example, they have realized scale benefits, their management system tends to more mature, the economic value of their brands is improved and they have created great economic benefits. The paper also points out the stable development path for the cooperatives, that is, the cooperatives should cooperate with local villages, and take a path of combined village and cooperative development so that the cooperative members can get a stable income; the cooperatives can also cooperate with local leading enterprises, which can help the cooperatives to manage, sell and form their products brands, thus the cooperatives can get loans easily to solve the capital bottleneck of cooperative development and improve the members' incomes.展开更多
基金supported by the National Natural Science Foundation of China(No.82402470)Postdoctoral Science Foundation of China(No.2023M743207)+2 种基金Science and Technology Key Research Project of Henan Province(No.252102311187)Postdoctoral Fellowship Program of CPSF(No.GZC20232438)Major Science and Technology Projects in Henan Province(No.241110310600).
文摘Infections by Carbapenem-resistant Enterobacteriaceae(CRE)producing New Delhi metallo-β-lactamases(NDMs)that hydrolyze carbapenems,are difficult to treat.Considering the fact of barren antibiotic development pipeline nowadays,revitalizing carbapenems efficacy using antibiotic adjuvants represents a promising but as-yet-unrealized tactic in combating NDMs-producing CRE,because of their off-target distributions,weak permeability to the outer membrane(OM)barrier,and limited NDMs-inactivation ability.Here,a pathogen-anchoring nano electrosensitizer,is proposed for serving as electrodynamic therapy(EDT)-activatable antibiotic adjuvant to revitalize meropenem(MEM,a typical carbapenems),through layerby-layer assembly of platinum nanoclusters(PtNCs),MEM,and maltodextrin(MA).The MA corona of electro-driven nanosensitizer promotes the efficient anchoring of PtNCs and MEM onto the surface of pathogen.Triggered by a square-wave alternating current(AC),MEM is released,PtNCs-based EDT is activated,accompanied by the generation of electro-driven reactive oxygen(ROS)at the bacterial infectious microenvironment.Subsequently,the antibacterial efficacy of MEM is thereby restored against NDMs-producing CRE through the ROS-mediated cascading effect,including the OM destruction,MEM intracellular accumulation,and NDMs inactivation.By the cooperation of MA-mediated pathogen anchoring and EDT-induced dual action,the pathogen-anchoring nano electrosensitizer revitalizes MEM efficacy against clinically isolated NDMs-producing CRE in vitro,and in mice infection model,importantly,also slows down the development of higher-level resistance in NDMsproducing CRE.The pathogen-anchoring nano electrosensitizer can fully play the therapeutic potency of carbapenems,providing a promising approach to tackle NDMs producer-induced infections.
基金Supported by The Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences(1630012013015)
文摘This paper gives an overview of the distribution,member scale,production and operation of banana cooperatives in Hainan Province,and points out the market risk and natural risk faced by the production of banana cooperatives in Hainan Province. In order to promote the banana cooperatives to form new agricultural management system integrating organization and intensification,this paper puts forth the production and operation recommendations,such as joint production of banana cooperatives,timely planting of banana,brand management,and improvement of production and operation technical level.
基金Supported by the Special Funding Project "Economic Development of Tropical Industry Cooperatives" of the Welfare Science Research Institutes at State Level(1630012012025)
文摘The paper introduces the present development of farmers' specialized cooperatives in China, points out that although the cooperatives in China develop rapidly, they are generally small and their advantages cannot be given into full play, and then analyzes the development achievements of the cooperatives, for example, they have realized scale benefits, their management system tends to more mature, the economic value of their brands is improved and they have created great economic benefits. The paper also points out the stable development path for the cooperatives, that is, the cooperatives should cooperate with local villages, and take a path of combined village and cooperative development so that the cooperative members can get a stable income; the cooperatives can also cooperate with local leading enterprises, which can help the cooperatives to manage, sell and form their products brands, thus the cooperatives can get loans easily to solve the capital bottleneck of cooperative development and improve the members' incomes.
