Saccharification of lignocellulosic wastes is the bottleneck of different bio-based chemical industries.Using enzymes for saccharification of lignocellulosic materials has several advantages over using chemicals.In th...Saccharification of lignocellulosic wastes is the bottleneck of different bio-based chemical industries.Using enzymes for saccharification of lignocellulosic materials has several advantages over using chemicals.In the current work,the application of the Maximyze■ enzyme system,which is industrially used in papermaking,was investigated in the saccharification of paper sludge and fiber dust wastes from the tissue paper industry.For optimizing the saccharification process,the effects of the consistency%,enzyme loading,and incubation time were studied and optimized using the Response Surface Methodology.The effect of these factors on the weight loss of paper sludge and total sugars in the hydrolyzate was studied.High-Performance Liquid Chromatography(HPLC)was used to measure the sugars composition of the hydrolyzate.Under the optimized conditions,~90% and~66% of the fiber dust and paper sludge could be hydrolyzed into sugars,respectively.The sugar composition was 80.23% glucose,10.99% xylose,and 8.65% arabinose based on the total sugars in the case of fiber dust.In comparison,80.63% glucose,8.43% xylose,and 10.75% arabinose were detected in the case of paper sludge.The results showed the applicability of the Maximyze Rcommercial enzymes used in the paper industry for efficient saccharification of paper sludge and fiber dust.The presence of non-cellulosic materials in the paper sludge(residual ink,paper additives,and ash)didn’t affect the activity of the enzymes.The study also showed the potential use of fiber dust as a valuable and clean source of sugars that can be used to prepare different bio-based chemicals.展开更多
Recycling of paper sludge waste is crucial for establishing a sustainable green industry.This waste contains valuable sugars that can be converted into important chemicals such as ethanol,poly hydroxybutyrate,and lact...Recycling of paper sludge waste is crucial for establishing a sustainable green industry.This waste contains valuable sugars that can be converted into important chemicals such as ethanol,poly hydroxybutyrate,and lactic acid.However,the main challenge in obtaining sugars in high yield from paper sludge is the high crystallinity of cellulose,which hinders hydrolysis.To address this,pretreatment using phosphoric acid was optimized using response surface methodology to facilitate cellulose hydrolysis with minimal energy and chemicals.The created prediction model using the response surface method considered factors such as acid concentration(ranging from 60%to 85%),consistency(ranging from 4%to 10%),temperature(ranging from 25℃to 80℃),and time(ranging from 0.5 to 4 h).The results revealed that the model’s significant factors affecting the yield were acid concentration,reaction time,temperature,and the product of acid concentration and temperature,while the model’s significant factors affecting the crystallinity were the consistency,the temperature,and their product.The results showed that the optimum conditions for pretreatment were using an acid concentration of 64%,temperature of 25℃,consistency of 10%,and time of 30 min.The hydrolysis of the conditionally pretreated paper sludge resulted in a weight loss of 42%,compared to only 18%weight loss in non-pretreated paper sludge.Furthermore,the optimized conditions led to low levels of furfurals and acetic acid,which are undesirable by-products that can interfere with sugar fermentation.The total sugar obtained under the optimized conditions was 0.43 g glucose/g sample(10.46 g/L),while the contents of methyl furfural,furfural,and acetic acid were 21.65,235.7,and 4.57 mg/L,respectively.This study demonstrates the potential of phosphoric acid for pretreatment and hydrolysis of paper sludge,enabling efficient saccharification and the production of sugars with minimal undesired by-products.展开更多
基金funding of the current work by the Science,Technology,and Innovation Funding Authority(STDF),Egypt,project no.46104:“Recycling of sludge wastes from paper industry via green technologies”.
文摘Saccharification of lignocellulosic wastes is the bottleneck of different bio-based chemical industries.Using enzymes for saccharification of lignocellulosic materials has several advantages over using chemicals.In the current work,the application of the Maximyze■ enzyme system,which is industrially used in papermaking,was investigated in the saccharification of paper sludge and fiber dust wastes from the tissue paper industry.For optimizing the saccharification process,the effects of the consistency%,enzyme loading,and incubation time were studied and optimized using the Response Surface Methodology.The effect of these factors on the weight loss of paper sludge and total sugars in the hydrolyzate was studied.High-Performance Liquid Chromatography(HPLC)was used to measure the sugars composition of the hydrolyzate.Under the optimized conditions,~90% and~66% of the fiber dust and paper sludge could be hydrolyzed into sugars,respectively.The sugar composition was 80.23% glucose,10.99% xylose,and 8.65% arabinose based on the total sugars in the case of fiber dust.In comparison,80.63% glucose,8.43% xylose,and 10.75% arabinose were detected in the case of paper sludge.The results showed the applicability of the Maximyze Rcommercial enzymes used in the paper industry for efficient saccharification of paper sludge and fiber dust.The presence of non-cellulosic materials in the paper sludge(residual ink,paper additives,and ash)didn’t affect the activity of the enzymes.The study also showed the potential use of fiber dust as a valuable and clean source of sugars that can be used to prepare different bio-based chemicals.
文摘Recycling of paper sludge waste is crucial for establishing a sustainable green industry.This waste contains valuable sugars that can be converted into important chemicals such as ethanol,poly hydroxybutyrate,and lactic acid.However,the main challenge in obtaining sugars in high yield from paper sludge is the high crystallinity of cellulose,which hinders hydrolysis.To address this,pretreatment using phosphoric acid was optimized using response surface methodology to facilitate cellulose hydrolysis with minimal energy and chemicals.The created prediction model using the response surface method considered factors such as acid concentration(ranging from 60%to 85%),consistency(ranging from 4%to 10%),temperature(ranging from 25℃to 80℃),and time(ranging from 0.5 to 4 h).The results revealed that the model’s significant factors affecting the yield were acid concentration,reaction time,temperature,and the product of acid concentration and temperature,while the model’s significant factors affecting the crystallinity were the consistency,the temperature,and their product.The results showed that the optimum conditions for pretreatment were using an acid concentration of 64%,temperature of 25℃,consistency of 10%,and time of 30 min.The hydrolysis of the conditionally pretreated paper sludge resulted in a weight loss of 42%,compared to only 18%weight loss in non-pretreated paper sludge.Furthermore,the optimized conditions led to low levels of furfurals and acetic acid,which are undesirable by-products that can interfere with sugar fermentation.The total sugar obtained under the optimized conditions was 0.43 g glucose/g sample(10.46 g/L),while the contents of methyl furfural,furfural,and acetic acid were 21.65,235.7,and 4.57 mg/L,respectively.This study demonstrates the potential of phosphoric acid for pretreatment and hydrolysis of paper sludge,enabling efficient saccharification and the production of sugars with minimal undesired by-products.
