Objective:To investigate the effects of silkworm pupa(Bombyx mori) protein(SPP) on cell proliferation,apoptosis and energy metabolism in human colon cancer cells DLD-1.Methods:CCK-8 was used to detect cell proliferati...Objective:To investigate the effects of silkworm pupa(Bombyx mori) protein(SPP) on cell proliferation,apoptosis and energy metabolism in human colon cancer cells DLD-1.Methods:CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group(normal cultured DLD-1 cells) and SPP dose groups;Annexin-V/PI was applied to observe cell apoptosis;XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function.Results:Comparing with the control,SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested(P <0.01);flow cytometry showed that SPP significantly promoted apoptosis(P<0.05).Additionally,SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses.Conclusion:SPP can cause oxidative damage,promote apoptosis,and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells,which reveals that SPP has the potential to serve as the anti-cancer drugs in the future,but further experimental evidence is needed.展开更多
Three-dimensional concrete printing(3DCP)is increasingly being applied in harsh environments and isolated regions.However,the effective utilization of aeolian sand(AS)resources and by-products derived from arid zones ...Three-dimensional concrete printing(3DCP)is increasingly being applied in harsh environments and isolated regions.However,the effective utilization of aeolian sand(AS)resources and by-products derived from arid zones for 3DCP is yet to be fully realized.This study developed a three-dimensional(3D)printing composite using AS and magnesium oxychloride cement(MOC)from local materials.The effects of the mole ratio of MgO/MgCl_(2)and sand/binder(S/B)ratio on the mechanical properties such as water resistance,drying shrinkage strain,rheology,and printability,were investigated systematically.The results indicated that the optimal mole ratio of MgO/MgCl_(2),was 8,which yielded the desired mechanical performance and water resistance.Furthermore,the S/B ratio can be increased to three within the desired printability to increase the AS utilization rate.The rheological recovery and buildability of the 3D-printed MOC with AS were verified.These findings provide a promising strategy for construction in remote deserts.展开更多
文摘Objective:To investigate the effects of silkworm pupa(Bombyx mori) protein(SPP) on cell proliferation,apoptosis and energy metabolism in human colon cancer cells DLD-1.Methods:CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group(normal cultured DLD-1 cells) and SPP dose groups;Annexin-V/PI was applied to observe cell apoptosis;XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function.Results:Comparing with the control,SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested(P <0.01);flow cytometry showed that SPP significantly promoted apoptosis(P<0.05).Additionally,SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses.Conclusion:SPP can cause oxidative damage,promote apoptosis,and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells,which reveals that SPP has the potential to serve as the anti-cancer drugs in the future,but further experimental evidence is needed.
基金the National Natural Science Foundation of China(Grant Nos.52178198,52208239,and U20A20313)the Natural Science Foundation of Hebei(Nos.E2022202203,E2021202039,and E2022202041)the Natural Science Foundation of Tianjin(Nos.20JCYBJC00710 and 22JCQNJC00240).
文摘Three-dimensional concrete printing(3DCP)is increasingly being applied in harsh environments and isolated regions.However,the effective utilization of aeolian sand(AS)resources and by-products derived from arid zones for 3DCP is yet to be fully realized.This study developed a three-dimensional(3D)printing composite using AS and magnesium oxychloride cement(MOC)from local materials.The effects of the mole ratio of MgO/MgCl_(2)and sand/binder(S/B)ratio on the mechanical properties such as water resistance,drying shrinkage strain,rheology,and printability,were investigated systematically.The results indicated that the optimal mole ratio of MgO/MgCl_(2),was 8,which yielded the desired mechanical performance and water resistance.Furthermore,the S/B ratio can be increased to three within the desired printability to increase the AS utilization rate.The rheological recovery and buildability of the 3D-printed MOC with AS were verified.These findings provide a promising strategy for construction in remote deserts.
