Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approach...Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approaches,although minimally invasive,are often limited by insufficient tissue penetration,uncontrolled thermal damage,and narrow therapeutic windows.We introduce GHz high-repetition-rate pulsed lasers as a transformative modality for tumor ablation.This approach capitalizes on the thermal accumulation effect of GHz pulse trains,in which the pulse interval is significantly shorter than the thermal relaxation time of biological tissue.Such a regime enables efficient and localized heat deposition in tumor regions.By precisely tuning the repetition frequency,pulse duration,and energy density,we establish a dynamic“ablation-cooling”cycle:rapid energy delivery followed by transient inter-pulse cooling.This thermal modulation ensures sharply confined ablation zones with reduced collateral damage.Our systematic investigation of laser-tissue interaction parameters demonstrates that GHz lasers offer superior spatial selectivity,minimized off-target injury,and enhanced treatment safety,presenting a compelling rationale for clinical translation of this paradigm in precision photothermal oncology.展开更多
Objective: Hemp seed oil is perfect for most skin types;it moisturizes skin and protects it from inflammation, oxidation, and other causes of aging. The problem is that the Hemp oil-based products do not penetrate the...Objective: Hemp seed oil is perfect for most skin types;it moisturizes skin and protects it from inflammation, oxidation, and other causes of aging. The problem is that the Hemp oil-based products do not penetrate the skin;they remain on the skin’s surface. Recently researchers have been trying to prepare nano emulsions of hemp oil to facilitate its permeation to deep skin layers. In all techniques used today, surfactants are added to the emulsification process. These surfactants may cause unwanted skin side effects. In the present study, we prepare micronized Hemp (m-Hemp) without using any surfactants in the micronization process, thus avoiding the side effects associated with surfactant addition. Methods & Results: Particles size of m-Hemp was evaluated using electron microscopy. Various sizes of m-Hemp were found, the smallest being 100 nm in diameter. The antioxidation properties of m-Hemp were measured using the Electron Spin Resonance (ESR) technique and were found to be enhanced. Skin topography and morphology following a cream containing m-Hemp treatment were visualized by Optical Profilometry and ESEM respectively. The results show a marked improvement in skin topography in all measured parameters. In addition, human keratinocytes (HaCaT) were exposed to inflammatory conditions and were then treated using Hemp. As a result, one of the key inflammatory factors (IL-2) was significantly reduced after treatment with m-Hemp (p ≤ 0.0001). The skin penetration of the cream containing m-Hemp was tested on human skin using the IMOPE (Iterative Multi-plane Optical Property Extraction) system. The results indicate that m-Hemp penetrates both the stratum corneum and the deep epidermal layers towards the dermis. Conclusion: The new cream prepared with micronized Hemp shows significant anti-inflammatory and antioxidative effects and demonstrates the entrance of m-Hemp to the skin epidermal layer.展开更多
In this study, we developed a highly sensitive dual-mode imaging system using gold nanoparticles （GNPs） conjugated to various fluorophores in solid phantoms. The system consists of fluorescence-lifetime imaging micr...In this study, we developed a highly sensitive dual-mode imaging system using gold nanoparticles （GNPs） conjugated to various fluorophores in solid phantoms. The system consists of fluorescence-lifetime imaging microscopy （FLIM） for surface imaging, diffusion reflection （DR） for deep-tissue imaging （up to 1 cm）, and metal-enhanced fluorescence （MEF）. We detected quenching in the fluorescent intensity （FI） for the conjugation of both gold nanospheres （GNS） and gold nanorods （GNRs） to Fluorescein, which has an excitation peak at a wavelength shorter than the surface plasmon resonance （SPR） of both types of GNPs. Enhanced FI was detected in conjugation to Rhodamine B （RhB） and Sulforhodamine B （SRB）, both with excitation peaks in the SPR regions of the GNPs. The enhanced FI was detected both in solution and in solid phantoms by the FLIM measurements. DR measurements detected the presence of GNRs within the solid phantoms by recording the dropped rates of light scattering in wavelengths corresponding to the absorption spectra of the GNRs. With the inclusion of MEF, this promising dual-mode imaging technique enables efficient and sensitive molecular and functional imaging.展开更多
Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing na...Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing nanohybrids composed of noble metals and fluorescent materials is a new way to perform multimodal imaging to overcome the limitations of single-modality counterparts. Herein, we propose the novel design of a multimodal contrast agent; namely, an enhanced nanohybrid comprising gold nanorods （GNRs） and carbon dots （CDs） with silica （SiO2） as a bridge. The nanohybrid （GNR@SiO2@CD） construction is based on covalent bonding between SiO2 and the silane-functionalized CDs, which links the GNRs with the CDs to form typical core-shell units. The novel structure not only retains and even highly improves the optical properties of the GNRs and CDs, but also possesses superior imaging performance in both diffusion reflection （DR） and fluorescence lifetime imaging microscopy （FLIM） measurements compared with bare GNRs or fluorescence dyes and CDs. The superior bioimaging properties of the GNR@SiOa@CD nanohybrids were successfully exploited for in vitro DR and FLIM measurements of macrophages within tissue-like phantoms, paving the way toward a theranostic contrast agent for atherosclerosis and cancer.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2022YFB3207204)the National Natural Science Foundation of China(Grant No.52293422)+6 种基金the Basic and Applied Basic Research Foundation of Guangdong Province-Regional Joint Fund-Key Projects(Grant Nos.2022B1515120012 and 2023B0101200003)the Department of Science and Technology of Guangdong Province(Grant No.2023B0101200003)the Science and Technology Innovation Commission of Shenzhen(Grant Nos.JCYJ20240813141317023,KJZD20240903095707010,KCXFZ20230731093259009,JCYJ20220818102618040,GJHZ20220913143207014,JCYJ20241202130558075,and KJZD20230923114002005)the Shenzhen Medical Research Fund(Grant Nos.D2301014 and D2402002)the Chemical Department of Hangzhou Normal University and the Ministry of Education Key Laboratory Open Scientific Projects Fund(Grant No.KFJJ2023007)the Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University,the Research Team Cultivation Program of Shenzhen University(Grant No.2023QNT008)the Graduate Independent Innovation Achievement Cultivation Project of Shenzhen University in 2025(Grant No.315-000066010715).
文摘Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approaches,although minimally invasive,are often limited by insufficient tissue penetration,uncontrolled thermal damage,and narrow therapeutic windows.We introduce GHz high-repetition-rate pulsed lasers as a transformative modality for tumor ablation.This approach capitalizes on the thermal accumulation effect of GHz pulse trains,in which the pulse interval is significantly shorter than the thermal relaxation time of biological tissue.Such a regime enables efficient and localized heat deposition in tumor regions.By precisely tuning the repetition frequency,pulse duration,and energy density,we establish a dynamic“ablation-cooling”cycle:rapid energy delivery followed by transient inter-pulse cooling.This thermal modulation ensures sharply confined ablation zones with reduced collateral damage.Our systematic investigation of laser-tissue interaction parameters demonstrates that GHz lasers offer superior spatial selectivity,minimized off-target injury,and enhanced treatment safety,presenting a compelling rationale for clinical translation of this paradigm in precision photothermal oncology.
文摘Objective: Hemp seed oil is perfect for most skin types;it moisturizes skin and protects it from inflammation, oxidation, and other causes of aging. The problem is that the Hemp oil-based products do not penetrate the skin;they remain on the skin’s surface. Recently researchers have been trying to prepare nano emulsions of hemp oil to facilitate its permeation to deep skin layers. In all techniques used today, surfactants are added to the emulsification process. These surfactants may cause unwanted skin side effects. In the present study, we prepare micronized Hemp (m-Hemp) without using any surfactants in the micronization process, thus avoiding the side effects associated with surfactant addition. Methods & Results: Particles size of m-Hemp was evaluated using electron microscopy. Various sizes of m-Hemp were found, the smallest being 100 nm in diameter. The antioxidation properties of m-Hemp were measured using the Electron Spin Resonance (ESR) technique and were found to be enhanced. Skin topography and morphology following a cream containing m-Hemp treatment were visualized by Optical Profilometry and ESEM respectively. The results show a marked improvement in skin topography in all measured parameters. In addition, human keratinocytes (HaCaT) were exposed to inflammatory conditions and were then treated using Hemp. As a result, one of the key inflammatory factors (IL-2) was significantly reduced after treatment with m-Hemp (p ≤ 0.0001). The skin penetration of the cream containing m-Hemp was tested on human skin using the IMOPE (Iterative Multi-plane Optical Property Extraction) system. The results indicate that m-Hemp penetrates both the stratum corneum and the deep epidermal layers towards the dermis. Conclusion: The new cream prepared with micronized Hemp shows significant anti-inflammatory and antioxidative effects and demonstrates the entrance of m-Hemp to the skin epidermal layer.
文摘In this study, we developed a highly sensitive dual-mode imaging system using gold nanoparticles （GNPs） conjugated to various fluorophores in solid phantoms. The system consists of fluorescence-lifetime imaging microscopy （FLIM） for surface imaging, diffusion reflection （DR） for deep-tissue imaging （up to 1 cm）, and metal-enhanced fluorescence （MEF）. We detected quenching in the fluorescent intensity （FI） for the conjugation of both gold nanospheres （GNS） and gold nanorods （GNRs） to Fluorescein, which has an excitation peak at a wavelength shorter than the surface plasmon resonance （SPR） of both types of GNPs. Enhanced FI was detected in conjugation to Rhodamine B （RhB） and Sulforhodamine B （SRB）, both with excitation peaks in the SPR regions of the GNPs. The enhanced FI was detected both in solution and in solid phantoms by the FLIM measurements. DR measurements detected the presence of GNRs within the solid phantoms by recording the dropped rates of light scattering in wavelengths corresponding to the absorption spectra of the GNRs. With the inclusion of MEF, this promising dual-mode imaging technique enables efficient and sensitive molecular and functional imaging.
基金This work was supported by the Joint NSFC-ISF Research Program （No. 51561145004）, jointly funded by the National Natural Science Foundation of China and the Israel Science Foundation, and the President＇s International Fellowship Initiative, Chinese Academy of Sciences （No. PIFI2015VTB041）.
文摘Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing nanohybrids composed of noble metals and fluorescent materials is a new way to perform multimodal imaging to overcome the limitations of single-modality counterparts. Herein, we propose the novel design of a multimodal contrast agent; namely, an enhanced nanohybrid comprising gold nanorods （GNRs） and carbon dots （CDs） with silica （SiO2） as a bridge. The nanohybrid （GNR@SiO2@CD） construction is based on covalent bonding between SiO2 and the silane-functionalized CDs, which links the GNRs with the CDs to form typical core-shell units. The novel structure not only retains and even highly improves the optical properties of the GNRs and CDs, but also possesses superior imaging performance in both diffusion reflection （DR） and fluorescence lifetime imaging microscopy （FLIM） measurements compared with bare GNRs or fluorescence dyes and CDs. The superior bioimaging properties of the GNR@SiOa@CD nanohybrids were successfully exploited for in vitro DR and FLIM measurements of macrophages within tissue-like phantoms, paving the way toward a theranostic contrast agent for atherosclerosis and cancer.
