The insecticidal protein produced by Pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and ...The insecticidal protein produced by Pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and fat body, and kills locusts. The disinsection rates of feeding and injection are 63.3% and 65.7%, respectively. After 24h to 48h, it is observed that all these tissues and cells show pathological changes in varying degrees, and so did the host cellular organs of these cells, such as cytoblast, mitochondria, endoplasmic reticulum and ribosome. Particularly, the changes of the mitochondria are much more serious than those of others. Detection of oxygen electrode shows the efficiency of oxidative phosphorylation and the ATP synthesis decreases, while the activity of mitochondrial ATPase is almost not affected. That means the energy utilization of locusts is in gear, but the shortage of the supplying of energy results in their death. This research forms the substantial basis for controlling locusts.展开更多
Budget is a means of competition in the external environment of enterprises. Adopting reasonable budget can achieve the goal of controlling the cost of enterprises and integrate it into many different businesses. The ...Budget is a means of competition in the external environment of enterprises. Adopting reasonable budget can achieve the goal of controlling the cost of enterprises and integrate it into many different businesses. The budget has a strong covering ability, which can cover many aspects such as business investment, financial statements and so on. At present, the comprehensive budget has become an important control means in the future enterprise development process, which plays a certain planning role in promoting enterprise operation and development. This paper will analyze the actual development and existing problems of enterprises in the process of budget management, and put forward specific solutions combined with the problems.展开更多
文摘The insecticidal protein produced by Pseudomonas pseudoalcaligenes is purified from the suspension of the bacterial culture. As an intact molecule, the protein acts on the foregut, midgut, hindgut, vasa Malpighii and fat body, and kills locusts. The disinsection rates of feeding and injection are 63.3% and 65.7%, respectively. After 24h to 48h, it is observed that all these tissues and cells show pathological changes in varying degrees, and so did the host cellular organs of these cells, such as cytoblast, mitochondria, endoplasmic reticulum and ribosome. Particularly, the changes of the mitochondria are much more serious than those of others. Detection of oxygen electrode shows the efficiency of oxidative phosphorylation and the ATP synthesis decreases, while the activity of mitochondrial ATPase is almost not affected. That means the energy utilization of locusts is in gear, but the shortage of the supplying of energy results in their death. This research forms the substantial basis for controlling locusts.
文摘Budget is a means of competition in the external environment of enterprises. Adopting reasonable budget can achieve the goal of controlling the cost of enterprises and integrate it into many different businesses. The budget has a strong covering ability, which can cover many aspects such as business investment, financial statements and so on. At present, the comprehensive budget has become an important control means in the future enterprise development process, which plays a certain planning role in promoting enterprise operation and development. This paper will analyze the actual development and existing problems of enterprises in the process of budget management, and put forward specific solutions combined with the problems.
