Many of the plants of genus Salsola are edible halophytes which are known for their use in treatment of inflammation as well as their nutritional content.This study aims at comprehensive chemical profiling of two Sals...Many of the plants of genus Salsola are edible halophytes which are known for their use in treatment of inflammation as well as their nutritional content.This study aims at comprehensive chemical profiling of two Salsola species that are widely distributed in arid areas;S.imbricata and S.jordanicola,using UHPLC-QqQ-MS in an attempt to decipher the bioactive constituents of the aerial parts of the selected plants and their ex-vivo antiinflammatory activity.73 chromatographic peaks were annotated where phytosterols dominated the metabolites in S.imbracta while phenylalkylamines were the major constituents detected in S.jordanicola.OPLS-DA model coefficients plot indicated that tetradecanoic acid,beta sitosterol and pentacosane were positively correlated to discrimination of S.imbricata species while N-feruloyloctopamine,N-feruloyltyramine,and ferulic acid,were positively correlated to discrimination of S.jordanicola species.Meanwhile,fractions of S.imbracta,reduced the 5 upregulation of TNF-αcaused by LPS to levels lower than those produced by piroxicam while the petroleum ether and n-butanol fraction of S.jordanicola were more effective than piroxicam in reducing IL-6 gene expression.The coefficients plot depicted that methyl palmitate,aegicin,cleomiscoside,beta sitosterol and sitostanol possessed strong positive correlation to the down regulation of the pro-inflammatory marker TNF-α,IL-1βand IFN-γwhile only norepinephrine derivatives were the major metabolites that showed strong positive correlation to the inhibition of IL-6 pro-inflammatory marker.The results obtained may help to explain chemically,the potential anti-inflammatory effect of the various Salsola species fractions increasing the value of the Salsola species as a putative functional foods.展开更多
Olibanum is a resinous extract extracted from several species of Boswellia trees.This works aims to contribute to the research on the chemistry and anti-inflammatory activity of raw,acidic and neutral fractions of dif...Olibanum is a resinous extract extracted from several species of Boswellia trees.This works aims to contribute to the research on the chemistry and anti-inflammatory activity of raw,acidic and neutral fractions of different olibanum species to determine the effects of different extraction procedures on the composition of anti-inflammatory constituents.Chemical profiling of different fractions of Boswellia species was attempted using UPLC/MS/MS coupled to chemometric analysis.The anti-inflammatory activity of the tested extracts was assessed on LPS-stimulated WBCs through four pro-inflammatory cytokines(TNF-α,IL-6,IL-1βand IFN-γ)in addition to reveal the efficacy-directed markers using multivariate analysis.UPLC/MS/MS analysis led to the annotation of 77 compounds belonging to several chemical classes.Chemometric models indicated that samples segregation was based on the geographical source of the oleo-gum-resin rather than extraction procedure.Acidic and neutral extracts of B.carterii and B.sacra alcoholic extract were strongly correlated to inhibition of the pro-inflammatory markers IL-1βand TNF-αwhile there was a strong correlation between the raw extract of B.frereana and the alcoholic extract of B.serrata activity to IFN-γinhibition.Meanwhile,the most active extracts against IL-6 were the acidic and raw extracts of B.serrata and B.frereana.展开更多
Marine algae are well-acknowledged as a prime store of largely unexplored extraordinary chemical entities potentially ameliorating against inflammation however,the efficacy mechanism remains elusive.This study offers ...Marine algae are well-acknowledged as a prime store of largely unexplored extraordinary chemical entities potentially ameliorating against inflammation however,the efficacy mechanism remains elusive.This study offers an integrated strategy of several bioinformatics and computational tools as well as bio-guided fractionation,and multi-step experimental validations integrated with metabolomics to systematically provide a shortcut of potentially active anti-inflammatory biomarkers of seaweeds.Firstly,multi-level network model of“multi-components-multi-targets-multiple pathways”was established.Complementarily,an in vitro anti-inflammatory testing was estimated via monitoring release of pro-inflammatory mediators.Bioassay-guided fractionation of S.platensis integrated with UPLC-MS analysis of the most promising enriched fractions in terms of anti-inflammatory efficacy were undertaken.Molecular docking studies on the top enriched targets were ultimately conducted.Multi-level network analysis illuminated that the intricate pharmacological mechanisms underlying inflammation mitigation was the knockout of the crucial targets of TLR2,AKT1 and PRKCA and further regulation of principal pathways as PI3K-Akt signaling pathway,arachidonic acid(AA)metabolism and Toll-like receptor signaling pathway.In vitro testing,S.platensis exhibited the greatest potency.UPLC-MS analysis of Spirulina active fractions showed linolenic acid,hydroxy linolenic acid,eicosapentaenoic acid,catechin and carnosic acid were efficacy constituents for mitigating inflammation.Molecular docking showed that eicosapentaenoic,hydroxy linolenic acid and carnosic acid displayed favorable molecular binding capacity to TLR2,AKT1 and PRKCA.Our rationally developed workflow offered the convincing evidence marine algae armed with an arsenal of weapons to regain the harmony disturbed in inflammatory disorders by multi-targeted synergistic functions.展开更多
文摘Many of the plants of genus Salsola are edible halophytes which are known for their use in treatment of inflammation as well as their nutritional content.This study aims at comprehensive chemical profiling of two Salsola species that are widely distributed in arid areas;S.imbricata and S.jordanicola,using UHPLC-QqQ-MS in an attempt to decipher the bioactive constituents of the aerial parts of the selected plants and their ex-vivo antiinflammatory activity.73 chromatographic peaks were annotated where phytosterols dominated the metabolites in S.imbracta while phenylalkylamines were the major constituents detected in S.jordanicola.OPLS-DA model coefficients plot indicated that tetradecanoic acid,beta sitosterol and pentacosane were positively correlated to discrimination of S.imbricata species while N-feruloyloctopamine,N-feruloyltyramine,and ferulic acid,were positively correlated to discrimination of S.jordanicola species.Meanwhile,fractions of S.imbracta,reduced the 5 upregulation of TNF-αcaused by LPS to levels lower than those produced by piroxicam while the petroleum ether and n-butanol fraction of S.jordanicola were more effective than piroxicam in reducing IL-6 gene expression.The coefficients plot depicted that methyl palmitate,aegicin,cleomiscoside,beta sitosterol and sitostanol possessed strong positive correlation to the down regulation of the pro-inflammatory marker TNF-α,IL-1βand IFN-γwhile only norepinephrine derivatives were the major metabolites that showed strong positive correlation to the inhibition of IL-6 pro-inflammatory marker.The results obtained may help to explain chemically,the potential anti-inflammatory effect of the various Salsola species fractions increasing the value of the Salsola species as a putative functional foods.
文摘Olibanum is a resinous extract extracted from several species of Boswellia trees.This works aims to contribute to the research on the chemistry and anti-inflammatory activity of raw,acidic and neutral fractions of different olibanum species to determine the effects of different extraction procedures on the composition of anti-inflammatory constituents.Chemical profiling of different fractions of Boswellia species was attempted using UPLC/MS/MS coupled to chemometric analysis.The anti-inflammatory activity of the tested extracts was assessed on LPS-stimulated WBCs through four pro-inflammatory cytokines(TNF-α,IL-6,IL-1βand IFN-γ)in addition to reveal the efficacy-directed markers using multivariate analysis.UPLC/MS/MS analysis led to the annotation of 77 compounds belonging to several chemical classes.Chemometric models indicated that samples segregation was based on the geographical source of the oleo-gum-resin rather than extraction procedure.Acidic and neutral extracts of B.carterii and B.sacra alcoholic extract were strongly correlated to inhibition of the pro-inflammatory markers IL-1βand TNF-αwhile there was a strong correlation between the raw extract of B.frereana and the alcoholic extract of B.serrata activity to IFN-γinhibition.Meanwhile,the most active extracts against IL-6 were the acidic and raw extracts of B.serrata and B.frereana.
文摘Marine algae are well-acknowledged as a prime store of largely unexplored extraordinary chemical entities potentially ameliorating against inflammation however,the efficacy mechanism remains elusive.This study offers an integrated strategy of several bioinformatics and computational tools as well as bio-guided fractionation,and multi-step experimental validations integrated with metabolomics to systematically provide a shortcut of potentially active anti-inflammatory biomarkers of seaweeds.Firstly,multi-level network model of“multi-components-multi-targets-multiple pathways”was established.Complementarily,an in vitro anti-inflammatory testing was estimated via monitoring release of pro-inflammatory mediators.Bioassay-guided fractionation of S.platensis integrated with UPLC-MS analysis of the most promising enriched fractions in terms of anti-inflammatory efficacy were undertaken.Molecular docking studies on the top enriched targets were ultimately conducted.Multi-level network analysis illuminated that the intricate pharmacological mechanisms underlying inflammation mitigation was the knockout of the crucial targets of TLR2,AKT1 and PRKCA and further regulation of principal pathways as PI3K-Akt signaling pathway,arachidonic acid(AA)metabolism and Toll-like receptor signaling pathway.In vitro testing,S.platensis exhibited the greatest potency.UPLC-MS analysis of Spirulina active fractions showed linolenic acid,hydroxy linolenic acid,eicosapentaenoic acid,catechin and carnosic acid were efficacy constituents for mitigating inflammation.Molecular docking showed that eicosapentaenoic,hydroxy linolenic acid and carnosic acid displayed favorable molecular binding capacity to TLR2,AKT1 and PRKCA.Our rationally developed workflow offered the convincing evidence marine algae armed with an arsenal of weapons to regain the harmony disturbed in inflammatory disorders by multi-targeted synergistic functions.
