Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span st...Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> were finely grinded and fractionated</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> by sieving into four granulometric classes (<100 μm, 100 - 200 μm, 200 - 355 μm and >355 μm). The obtained powder fractions were used for essential oil (EO) extraction by hydrodistillation and their phytochemical profile and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activities were evaluated. The mother powder (unsieved powder) was used for comparison. Particle size exerted a significant influence (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.05) on the phytochemical composition and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant prop</span><span><span style="font-family:Verdana;">erties of the EOs. Comparatively, the mother powder had the highest contents of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-pinene (55.6%), camphene (3.4%) and limonene (3.7%), while 1,8-cineole (26.6% and 22.4%), </span></span><span style="font-family:Verdana;">exo-fenchol (5.6% and 3.5%), </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-campholenol (4.2% and 3.4%), L-trans-pinocarveol (5.5% and 2.7%), L-borneol (12.6% and 6.8%) and </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-terpineol (16.4% and 7.6%) are the main compounds of EOs from the <100 μm and 100 - 200 μm fractions, respectively. The antioxidant activities of the EOs revealed higher radical-scavenging activities DPPH (90.62% and 70.46%) and ABTS (89.59% and 73.31%) for finer fractions (<100 μm and 100 - 200 μm, respectively). The best reducing power (36.15% and 34.27%) were also found in these finer powder fractions which improved by more than 2 times the value of mother powder (reducing power of 17.01%). These results suggest that grinding followed by sieve fractionation concentrates the majority of antioxidant phytochemicals in the EOs of the finer powder fractions of </span><i><span style="font-family:Verdana;">E.</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> leaves. Finer powders could be used as functional ingredients in food formulations for the management of chronic diseases.</span></span>展开更多
The revaluation of medicinal plants in the veterinary pharmacopoeia is essential for the development of livestock in Burkina Faso. In order to justify the use of C. sesamoides and S. hermonthica in the treatment of ga...The revaluation of medicinal plants in the veterinary pharmacopoeia is essential for the development of livestock in Burkina Faso. In order to justify the use of C. sesamoides and S. hermonthica in the treatment of gastrointestinal parasitosis of small ruminants, a quantification of phenolic compounds as well as antioxidant activity and acute toxicité evaluation of both plants were performed. Acute toxicity was evaluated by administering a single dose of 2000 mg/kg body weight of aqueous extract of both plants to mice. The highest total polyphenol content for C. sesamo?des was obtained in the ethyl acetate fraction with 47.236 ± 2.57 mgEAG/100mg while that of S. hermonthica was 74.871 ± 2.57 mgEAG/100mg obtained with the n-butanol Fraction. The dichloromethane extract of S. hermonthica obtained the highest total flavonoid content with 7.31 ± 0.48 mgEQ/100 while the highest total flavonoid content of C. sesamo?des was obtained with ethyl acetate fraction with 5.4273 ± 0.35 mgEQ/100mg. The aqueous extract of C. sesamo?des obtained the highest content of condensed tannins with 3.028 ± 0.13 mgEAT/100mg. Both plant’s extracts did not show any signs of toxicity in NMRI mice after administration of the dose of 2000 mg/kg. The antioxidant activity by DPPH, FRAP and ABTS methods was good compared to those of Quercetin and Trolox taken as standard.展开更多
Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential ...Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential of Eucalyptus camaldulensis essential oils (EO) on CCl<sub>4</sub>-induced hepatic damage in Wistar rats. Animals were daily orally treated with the EOs extracted by hydrodistillation from powder sieved at four particle sizes (≥355, 200 - 355, 100 - 200, ≤100 μm) and those of the unsieved powder at dose of 50 mg/Kg for 7 days. Compounds that are evaluated for these activities are hydrocarbons and oxygenated terpenes that were identified and quantified by GC/MS. Activities of enzymes markers of hepatocellular damage in serum and antioxidant enzymes in the liver homogenates were measured. In this research, EOs significantly prevented the increase in serum ALT and AST (p < 0.05), total cholesterol, triglyceride and LDL-cholesterol level in acute liver damage induced by CCl<sub>4</sub> and significant increase level of plasma HDL-cholesterol. Also, significantly (p < 0.05) decreased the extent of malondialdehyde (MDA) formation and elevated the activities of superoxide dismutase (SOD) and catalase (CAT) liver in comparison to negative control group. The best antioxidant and hepatoprotective activities were those of EOs from two fine powder fractions (≤100 μm and 100 - 200 μm) was correlated to their high concentration in oxygenated terpenes (70.9% and 46.4%, respectively), when compared to the large particles (200 - 355 μm and ≥355 μm, with 33.3% and 41.8%, respectively) and unsieved powder (37.4%).展开更多
文摘Dried leaves of <i><span style="font-family:Verdana;">Eucalyptus</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> were finely grinded and fractionated</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> by sieving into four granulometric classes (<100 μm, 100 - 200 μm, 200 - 355 μm and >355 μm). The obtained powder fractions were used for essential oil (EO) extraction by hydrodistillation and their phytochemical profile and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant activities were evaluated. The mother powder (unsieved powder) was used for comparison. Particle size exerted a significant influence (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < 0.05) on the phytochemical composition and </span><i><span style="font-family:Verdana;">in</span></i> <i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antioxidant prop</span><span><span style="font-family:Verdana;">erties of the EOs. Comparatively, the mother powder had the highest contents of </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-pinene (55.6%), camphene (3.4%) and limonene (3.7%), while 1,8-cineole (26.6% and 22.4%), </span></span><span style="font-family:Verdana;">exo-fenchol (5.6% and 3.5%), </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-campholenol (4.2% and 3.4%), L-trans-pinocarveol (5.5% and 2.7%), L-borneol (12.6% and 6.8%) and </span><i><span style="font-family:Verdana;">α</span></i><span style="font-family:Verdana;">-terpineol (16.4% and 7.6%) are the main compounds of EOs from the <100 μm and 100 - 200 μm fractions, respectively. The antioxidant activities of the EOs revealed higher radical-scavenging activities DPPH (90.62% and 70.46%) and ABTS (89.59% and 73.31%) for finer fractions (<100 μm and 100 - 200 μm, respectively). The best reducing power (36.15% and 34.27%) were also found in these finer powder fractions which improved by more than 2 times the value of mother powder (reducing power of 17.01%). These results suggest that grinding followed by sieve fractionation concentrates the majority of antioxidant phytochemicals in the EOs of the finer powder fractions of </span><i><span style="font-family:Verdana;">E.</span></i> <i><span style="font-family:Verdana;">camaldulensis</span></i><span style="font-family:Verdana;"> leaves. Finer powders could be used as functional ingredients in food formulations for the management of chronic diseases.</span></span>
文摘The revaluation of medicinal plants in the veterinary pharmacopoeia is essential for the development of livestock in Burkina Faso. In order to justify the use of C. sesamoides and S. hermonthica in the treatment of gastrointestinal parasitosis of small ruminants, a quantification of phenolic compounds as well as antioxidant activity and acute toxicité evaluation of both plants were performed. Acute toxicity was evaluated by administering a single dose of 2000 mg/kg body weight of aqueous extract of both plants to mice. The highest total polyphenol content for C. sesamo?des was obtained in the ethyl acetate fraction with 47.236 ± 2.57 mgEAG/100mg while that of S. hermonthica was 74.871 ± 2.57 mgEAG/100mg obtained with the n-butanol Fraction. The dichloromethane extract of S. hermonthica obtained the highest total flavonoid content with 7.31 ± 0.48 mgEQ/100 while the highest total flavonoid content of C. sesamo?des was obtained with ethyl acetate fraction with 5.4273 ± 0.35 mgEQ/100mg. The aqueous extract of C. sesamo?des obtained the highest content of condensed tannins with 3.028 ± 0.13 mgEAT/100mg. Both plant’s extracts did not show any signs of toxicity in NMRI mice after administration of the dose of 2000 mg/kg. The antioxidant activity by DPPH, FRAP and ABTS methods was good compared to those of Quercetin and Trolox taken as standard.
文摘Particle size fractionation by sieving is used to optimize antioxydant potential of natural substances. The aim of the present study was to evaluate particle size effects on antioxidant and hepatoprotective potential of Eucalyptus camaldulensis essential oils (EO) on CCl<sub>4</sub>-induced hepatic damage in Wistar rats. Animals were daily orally treated with the EOs extracted by hydrodistillation from powder sieved at four particle sizes (≥355, 200 - 355, 100 - 200, ≤100 μm) and those of the unsieved powder at dose of 50 mg/Kg for 7 days. Compounds that are evaluated for these activities are hydrocarbons and oxygenated terpenes that were identified and quantified by GC/MS. Activities of enzymes markers of hepatocellular damage in serum and antioxidant enzymes in the liver homogenates were measured. In this research, EOs significantly prevented the increase in serum ALT and AST (p < 0.05), total cholesterol, triglyceride and LDL-cholesterol level in acute liver damage induced by CCl<sub>4</sub> and significant increase level of plasma HDL-cholesterol. Also, significantly (p < 0.05) decreased the extent of malondialdehyde (MDA) formation and elevated the activities of superoxide dismutase (SOD) and catalase (CAT) liver in comparison to negative control group. The best antioxidant and hepatoprotective activities were those of EOs from two fine powder fractions (≤100 μm and 100 - 200 μm) was correlated to their high concentration in oxygenated terpenes (70.9% and 46.4%, respectively), when compared to the large particles (200 - 355 μm and ≥355 μm, with 33.3% and 41.8%, respectively) and unsieved powder (37.4%).
