The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel m...The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.展开更多
The development of biosensors is gaining tremendous attention in various fields due to their extraordinary advantages, however, their sensitivity and accuracy are still challenging. Herein, we proposed a novel multifu...The development of biosensors is gaining tremendous attention in various fields due to their extraordinary advantages, however, their sensitivity and accuracy are still challenging. Herein, we proposed a novel multifunctional nanocomposite Ce-MOF@PdNPs (MOF = metal-organic framework, PdNPs = Pd nanoparticles)-mediated triple-readout aptasensor for accurate and reliable detection of diethylstilbestrol (DES), in which Ce-MOF@PdNPs exhibited excellent peroxidase (POD)-like activity, fluormetric, and electro conductive properties. In addition, enzymes-assisted target recycling amplification was utilized to improve the sensitivity, that is the specific binding of aptamer and DES triggered an Exo III enzyme-assisted recycling reaction. The generated F-DNA was captured by the H3 strand linked to Ce-MOF@PdNPs immobilized on the electrode, exposing cleavage sites and activating the Nt.BbvCI enzyme-assisted recycling reaction, leading to the dissociation of Ce-MOF@PdNPs and a significant reduced electrochemical signal. The collected Ce-MOF@PdNPs solution also induced a proportional change in the color and fluorescence, achieving a colorimetric and fluormetric detection functionality. The detection limit under colorimetric mode was 0.16 and 0.76 ng/mL under fluorescence mode, and 0.87 pg/mL under electrochemical mode. This triple-readout aptasensor exhibits high sensitivity, selectivity and accuracy, providing a new idea for designing novel biosensing platforms for veterinary drug residue detection.展开更多
基金supported by the National Natural Science Foundation of China(32272449)the Basic Research Program of Jiangsu(BK20240100)+1 种基金the Fundamental Research Funds for the Central Universities(JUSRP622025)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.
文摘The design of advanced dual-mode aptasensors has emerged as a promising strategy for the reliable detection of contaminants,with nanomaterial-mediated signal output playing a pivotal role.Herein,we developed a novel multifunctional nanocomposite material(FePOs@CeO_(2))-mediated colorimetric/electrochemical dual-mode aptasensor for the sensitive and specific detection of amoxicillin(AMO),a widely used semi-synthetic peni-cillin.Therein,FePOs@CeO_(2)relied on the catalytic ability of FePOs and the excellent electrochemical perfor-mance of cerium’s multivalent states(Ce^(3+)/Ce^(4+))to promote effective electron transfer at the electrode interface,thereby exhibiting excellent peroxidase(POD)like activity and conductive properties.Furthermore,an aptamer AMO-1 with specific binding affinity for AMO was obtained and its truncated derivative(AMO-1b)with enhanced binding affinity was obtained through molecular docking simulation and secondary structure analysis.The specific binding of this high-affinity aptamer to AMO caused FePOs@CeO_(2)to detach from the electrode surface,thereby triggering a change in the electrochemical signal.The collected FePOs@CeO_(2)solution induced a color change,thereby enabling the colorimetric detection function.The sensing platform demonstrated high sensitivity,specificity,and accuracy in the detection of AMO,with detection limits of 0.828μg/mL(colorimetric)and 7.24×10^(-5)μg/mL(electrochemical).In addition,the sensor exhibited good practicality in the detection of AMO in milk,with recovery rates of 97.49%-98.25%(colorimetric)and 92.24%-93.01%(electrochemical),respectively.The dual detection results could verify each other,which improved the accuracy and reliability of the results and provided a strategy for the design of a novel biosensing platform for veterinary drug residue detection.
基金supported by Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(22)3006)National Natural Science Foundation of China(Nos.32272449 and 32072310)+1 种基金the Fundamental Research Funds for the Central Universities(No.JUSRP622025)Collaborative innovation center of food safety and quality control in Jiangsu Province.
文摘The development of biosensors is gaining tremendous attention in various fields due to their extraordinary advantages, however, their sensitivity and accuracy are still challenging. Herein, we proposed a novel multifunctional nanocomposite Ce-MOF@PdNPs (MOF = metal-organic framework, PdNPs = Pd nanoparticles)-mediated triple-readout aptasensor for accurate and reliable detection of diethylstilbestrol (DES), in which Ce-MOF@PdNPs exhibited excellent peroxidase (POD)-like activity, fluormetric, and electro conductive properties. In addition, enzymes-assisted target recycling amplification was utilized to improve the sensitivity, that is the specific binding of aptamer and DES triggered an Exo III enzyme-assisted recycling reaction. The generated F-DNA was captured by the H3 strand linked to Ce-MOF@PdNPs immobilized on the electrode, exposing cleavage sites and activating the Nt.BbvCI enzyme-assisted recycling reaction, leading to the dissociation of Ce-MOF@PdNPs and a significant reduced electrochemical signal. The collected Ce-MOF@PdNPs solution also induced a proportional change in the color and fluorescence, achieving a colorimetric and fluormetric detection functionality. The detection limit under colorimetric mode was 0.16 and 0.76 ng/mL under fluorescence mode, and 0.87 pg/mL under electrochemical mode. This triple-readout aptasensor exhibits high sensitivity, selectivity and accuracy, providing a new idea for designing novel biosensing platforms for veterinary drug residue detection.
