Background:The present comprehensive protocol is focused on the detection of pathogenic enteric RNA viruses,explicitly focusing on norovirus genogroup II(GII),astrovirus,rotavirus,Aichi virus,sapovirus,hepatitis A and...Background:The present comprehensive protocol is focused on the detection of pathogenic enteric RNA viruses,explicitly focusing on norovirus genogroup II(GII),astrovirus,rotavirus,Aichi virus,sapovirus,hepatitis A and E viruses in wastewater treatment plants through droplet digital PCR(ddPCR).Enteric viruses are of significant public health concern,as they are the leading cause of diseases like gastroenteritis.Regular monitoring of environmental samples,particularly from wastewater treatment plants,is crucial for early detection and control of these viruses.This research aims to improve the understanding of the prevalence and dynamics of enteric viruses in urban India and will serve as a model for similar studies in other regions.Our protocol's objective is to establish a novel ddPCRbased methodology for the detection and molecular characterization of enteric viruses present in wastewater samples sourced from Bhopal,India.Our assay is capable of accurately quantifying virus concentrations without standard curves,minimizing extensive optimization,and enhancing sensitivity and precision,especially for low-abundance targets.Methods:The study involves fortnightly collecting and analyzing samples from nine wastewater treatment plants over two years,ensuring comprehensive coverage and consistent data.Our study innovatively applies ddPCR to simultaneously detect and quantify enteric viruses in wastewater,a more advanced technique.Additionally,we will employ next-generation sequencing for detailed viral genome identification in samples tested positive for pathogenic viruses.Conclusion:This study will aid in understanding these viruses’genetic diversity and mutation rates,which is crucial for developing tailored intervention strategies.The findings will be instrumental in shaping public health responses and improving epidemiological surveillance,especially in localities heaving sewage networks.展开更多
Type-2 diabetes mellitus(T2DM)is a global epidemic with significant societal costs.The gut microbiota,including its metabolites,plays a pivotal role in maintaining health,while gut dysbiosis is implicated in several m...Type-2 diabetes mellitus(T2DM)is a global epidemic with significant societal costs.The gut microbiota,including its metabolites,plays a pivotal role in maintaining health,while gut dysbiosis is implicated in several metabolic disorders,including T2DM.Although data exists on the relationship between the gut bacteriome and metabolic disorders,further attention is needed for the mycobiome and virome.Recent advancements have begun to shed light on these connections,offering potential avenues for preventive measures.However,more comprehensive investigations are required to untangle the interrelations between different microbial kingdoms and their role in T2DM development or mitigation.This review presents a simplified overview of the alterations in the gut bacteriome in T2DM and delves into the current understanding of the mycobiome and virome’s role in T2DM,along with their interactions with the cohabiting bacteriome.Subsequently,it explores into the age-related dynamics of the gut microbiome and the changes observed in the microbiome composition with the onset of T2DM.Further,we explore the basic workflow utilized in gut microbiome studies.Lastly,we discuss potential therapeutic interventions in gut microbiome research,which could contribute to the amelioration of the condition,serve as preventive measures,or pave the way towards personalized medicine.展开更多
文摘Background:The present comprehensive protocol is focused on the detection of pathogenic enteric RNA viruses,explicitly focusing on norovirus genogroup II(GII),astrovirus,rotavirus,Aichi virus,sapovirus,hepatitis A and E viruses in wastewater treatment plants through droplet digital PCR(ddPCR).Enteric viruses are of significant public health concern,as they are the leading cause of diseases like gastroenteritis.Regular monitoring of environmental samples,particularly from wastewater treatment plants,is crucial for early detection and control of these viruses.This research aims to improve the understanding of the prevalence and dynamics of enteric viruses in urban India and will serve as a model for similar studies in other regions.Our protocol's objective is to establish a novel ddPCRbased methodology for the detection and molecular characterization of enteric viruses present in wastewater samples sourced from Bhopal,India.Our assay is capable of accurately quantifying virus concentrations without standard curves,minimizing extensive optimization,and enhancing sensitivity and precision,especially for low-abundance targets.Methods:The study involves fortnightly collecting and analyzing samples from nine wastewater treatment plants over two years,ensuring comprehensive coverage and consistent data.Our study innovatively applies ddPCR to simultaneously detect and quantify enteric viruses in wastewater,a more advanced technique.Additionally,we will employ next-generation sequencing for detailed viral genome identification in samples tested positive for pathogenic viruses.Conclusion:This study will aid in understanding these viruses’genetic diversity and mutation rates,which is crucial for developing tailored intervention strategies.The findings will be instrumental in shaping public health responses and improving epidemiological surveillance,especially in localities heaving sewage networks.
基金supported/funded by the Indian Council of Medical Research DHR-ICMR/GIA/05/18/2020.
文摘Type-2 diabetes mellitus(T2DM)is a global epidemic with significant societal costs.The gut microbiota,including its metabolites,plays a pivotal role in maintaining health,while gut dysbiosis is implicated in several metabolic disorders,including T2DM.Although data exists on the relationship between the gut bacteriome and metabolic disorders,further attention is needed for the mycobiome and virome.Recent advancements have begun to shed light on these connections,offering potential avenues for preventive measures.However,more comprehensive investigations are required to untangle the interrelations between different microbial kingdoms and their role in T2DM development or mitigation.This review presents a simplified overview of the alterations in the gut bacteriome in T2DM and delves into the current understanding of the mycobiome and virome’s role in T2DM,along with their interactions with the cohabiting bacteriome.Subsequently,it explores into the age-related dynamics of the gut microbiome and the changes observed in the microbiome composition with the onset of T2DM.Further,we explore the basic workflow utilized in gut microbiome studies.Lastly,we discuss potential therapeutic interventions in gut microbiome research,which could contribute to the amelioration of the condition,serve as preventive measures,or pave the way towards personalized medicine.
