To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium and concentrations and ratios of plant growth regulators on tissue culture and rapid propaga...To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium and concentrations and ratios of plant growth regulators on tissue culture and rapid propagation of T.amurensis were studied.The results showed that 1/2 MS medium was the most suitable proliferation medium,and the proliferation coefficient could reach 13.5 after adding 0.05 mg/L 6-BA and 0.03 mg/L IBA;MS medium was the most suitable medium for strong plantlets and rooting,and the best medium for strong plantlets was MS+0.1 mg/L 6-BA+0.1 mg/L IBA+0.03 mg/L GA_(3),with which the average plantlet height reached 5.15 cm;and the best rooting medium was MS+1.0 mg/L6-BA+0.05 mg/L NAA,with which the rooting rate was 93.3%and the number of roots was 5.7 roots.展开更多
Ash trees(Fraxinus)exhibit rich genetic diversity and wide adaptation to various ecological environments,and several species are highly salt tolerant.Dissecting the genomic basis of salt adaptation in Fraxinus is vita...Ash trees(Fraxinus)exhibit rich genetic diversity and wide adaptation to various ecological environments,and several species are highly salt tolerant.Dissecting the genomic basis of salt adaptation in Fraxinus is vital for its resistance breeding.Here,we present 11 high-quality chromosome-level genome assemblies for Fraxinus species,which reveal two unequal subgenome compositions and two recent whole-genome triplication events in their evolutionary history.A Fraxinus pan-genome was constructed on the basis of structural variations and revealed that presence–absence variations(PAVs)of transmembrane transport genes have likely contributed to salt adaptation in Fraxinus.Through whole-genome resequencing of an F1 population from an interspecies cross of F.velutina‘Lula 3’(salt tolerant)with F.pennsylvanica‘Lula 5’(salt sensitive),we mapped salt-tolerance PAV-based quantitative trait loci(QTLs)and pinpointed two PAV-QTLs and candidate genes associated with Fraxinus salt tolerance.Mechanistically,FvbHLH85 enhances salt tolerance by mediating reactive oxygen species and Na^(+)/K^(+)homeostasis,whereas FvSWEET5 enhances salt tolerance by mediating osmotic homeostasis.Collectively,these findings provide valuable genomic resources for Fraxinus salt-resistance breeding and the research community.展开更多
Dear Editor,Colorectal cancer(CRC)is an important digestive tract tumor with high malignancy.Nearly one million people suffer from CRC every year with a mortality rate of 33%.Therefore,it is urgent to determine the me...Dear Editor,Colorectal cancer(CRC)is an important digestive tract tumor with high malignancy.Nearly one million people suffer from CRC every year with a mortality rate of 33%.Therefore,it is urgent to determine the mechanism for the development of CRC,in order to find more effective diagnosis markers and therapeutic targets.Various RNA modifications have been identified in both mRNA and non-coding RNAs,with RNA methylation being the most common mRNA modification in eukaryotes.展开更多
Let A and B be rings and U a(B,A)-bimodule.If BU is flat and UA is finitely generated projective(resp.,BU is finitely generated projective and UA is flat),then the characterizations of level modules and Gorenstein AC-...Let A and B be rings and U a(B,A)-bimodule.If BU is flat and UA is finitely generated projective(resp.,BU is finitely generated projective and UA is flat),then the characterizations of level modules and Gorenstein AC-projective modules(resp.,absolutely clean modules and Gorenstein AC-injective modules)over the formal triangular matrix ring T=(A0 UB)are given.As applications,it is proved that every Gorenstein AC-projective left T-module is projective if and only if each Gorenstein AC-projective left A-module and B-module is projective,and every Gorenstein AC-injective left T-module is injective if and only if each Gorenstein AC-injective left A-module and B-module is injective.Moreover,Gorenstein AC-projective and AC-injective dimensions over the formal triangular matrix ring T are studied.展开更多
基金Supported by Key R&D Program of Shandong Province(2017GNC10112)。
文摘To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium and concentrations and ratios of plant growth regulators on tissue culture and rapid propagation of T.amurensis were studied.The results showed that 1/2 MS medium was the most suitable proliferation medium,and the proliferation coefficient could reach 13.5 after adding 0.05 mg/L 6-BA and 0.03 mg/L IBA;MS medium was the most suitable medium for strong plantlets and rooting,and the best medium for strong plantlets was MS+0.1 mg/L 6-BA+0.1 mg/L IBA+0.03 mg/L GA_(3),with which the average plantlet height reached 5.15 cm;and the best rooting medium was MS+1.0 mg/L6-BA+0.05 mg/L NAA,with which the rooting rate was 93.3%and the number of roots was 5.7 roots.
基金supported by the Agriculture Seed Improvement Project of Shandong Province(2023LZGVC012 and 2019LZGC009).
文摘Ash trees(Fraxinus)exhibit rich genetic diversity and wide adaptation to various ecological environments,and several species are highly salt tolerant.Dissecting the genomic basis of salt adaptation in Fraxinus is vital for its resistance breeding.Here,we present 11 high-quality chromosome-level genome assemblies for Fraxinus species,which reveal two unequal subgenome compositions and two recent whole-genome triplication events in their evolutionary history.A Fraxinus pan-genome was constructed on the basis of structural variations and revealed that presence–absence variations(PAVs)of transmembrane transport genes have likely contributed to salt adaptation in Fraxinus.Through whole-genome resequencing of an F1 population from an interspecies cross of F.velutina‘Lula 3’(salt tolerant)with F.pennsylvanica‘Lula 5’(salt sensitive),we mapped salt-tolerance PAV-based quantitative trait loci(QTLs)and pinpointed two PAV-QTLs and candidate genes associated with Fraxinus salt tolerance.Mechanistically,FvbHLH85 enhances salt tolerance by mediating reactive oxygen species and Na^(+)/K^(+)homeostasis,whereas FvSWEET5 enhances salt tolerance by mediating osmotic homeostasis.Collectively,these findings provide valuable genomic resources for Fraxinus salt-resistance breeding and the research community.
基金funded by Outstanding Clinical Discipline Project of Shanghai Pudong(No.PWYgy-2018-08)Program for Medical Key Departments of Shanghai(No.ZK2019A10)+4 种基金Shanghai Key Laboratory of Vascular Lesions Regulation and Remodeling(No.zdsys202002)Committee of Shanghai Science and Technology(Nos.18ZR1433900 and 17DZ2200200)Discipline Construction Promoting Project of Shanghai Pudong Hospital(Zdxk2020-01)Academic Leaders Training Program Supported by Pudong Health Bureau of Shanghai(No.PWRd2016-05)Specialty Feature Construction Project of Pudong Health and Family Planning Commission of Shanghai(No.PWZzb2017-21).
文摘Dear Editor,Colorectal cancer(CRC)is an important digestive tract tumor with high malignancy.Nearly one million people suffer from CRC every year with a mortality rate of 33%.Therefore,it is urgent to determine the mechanism for the development of CRC,in order to find more effective diagnosis markers and therapeutic targets.Various RNA modifications have been identified in both mRNA and non-coding RNAs,with RNA methylation being the most common mRNA modification in eukaryotes.
基金partly supported by NSF of China(grants 11761047 and 11861043).
文摘Let A and B be rings and U a(B,A)-bimodule.If BU is flat and UA is finitely generated projective(resp.,BU is finitely generated projective and UA is flat),then the characterizations of level modules and Gorenstein AC-projective modules(resp.,absolutely clean modules and Gorenstein AC-injective modules)over the formal triangular matrix ring T=(A0 UB)are given.As applications,it is proved that every Gorenstein AC-projective left T-module is projective if and only if each Gorenstein AC-projective left A-module and B-module is projective,and every Gorenstein AC-injective left T-module is injective if and only if each Gorenstein AC-injective left A-module and B-module is injective.Moreover,Gorenstein AC-projective and AC-injective dimensions over the formal triangular matrix ring T are studied.
