The internal microstructures of rock materials, including mineral heterogeneity and intrinsic microdefects, exert a significant influence on their nonlinear mechanical and cracking behaviors. It is of great significan...The internal microstructures of rock materials, including mineral heterogeneity and intrinsic microdefects, exert a significant influence on their nonlinear mechanical and cracking behaviors. It is of great significance to accurately characterize the actual microstructures and their influence on stress and damage evolution inside the rocks. In this study, an image-based fast Fourier transform (FFT) method is developed for reconstructing the actual rock microstructures by combining it with the digital image processing (DIP) technique. A series of experimental investigations were conducted to acquire information regarding the actual microstructure and the mechanical properties. Based on these experimental evidences, the processed microstructure information, in conjunction with the proposed micromechanical model, is incorporated into the numerical calculation. The proposed image-based FFT method was firstly validated through uniaxial compression tests. Subsequently, it was employed to predict and analyze the influence of microstructure on macroscopic mechanical behaviors, local stress distribution and the internal crack evolution process in brittle rocks. The distribution of feldspar is considerably more heterogeneous and scattered than that of quartz, which results in a greater propensity for the formation of cracks in feldspar. It is observed that initial cracks and new cracks, including intragranular and boundary ones, ultimately coalesce and connect as the primary through cracks, which are predominantly distributed along the boundary of the feldspar. This phenomenon is also predicted by the proposed numerical method. The results indicate that the proposed numerical method provides an effective approach for analyzing, understanding and predicting the nonlinear mechanical and cracking behaviors of brittle rocks by taking into account the actual microstructure characteristics.展开更多
Semiconductor quantum dots(QDs),as high-performance materials,play an essential role in contemporary industry,mainly due to their high photoluminescent quantum yield,wide absorption characteristics,and size-dependent ...Semiconductor quantum dots(QDs),as high-performance materials,play an essential role in contemporary industry,mainly due to their high photoluminescent quantum yield,wide absorption characteristics,and size-dependent light emission.It is essential to construct well-defined micro-/nano-structures using QDs as building blocks for micro-optic applications.However,the fabrication of stable QDs with designed functional structures has long been challenging.Here,we propose a strategy for three-dimensional direct lithography of desired QDs within a hybrid medium with specific protection properties.The acrylate-functionalized hybrid precursors enable local crosslinking through ultrafast laser-induced multiphoton absorption,achieving sub-100 nm resolution surpassing the diffraction limit.The printed micro-/nano-structures possess thermal stability up to 600℃,which can be transformed to inorganic architectures with a volume shrinkage.Due to the encapsulated QDs within the densely silicon-oxygen molecular networks,the functional structures demonstrate good stability against ultraviolet irradiation,corrosive solutions,and elevated temperatures.Based on hybrid3D nanolithography,bicolor multilayer micro-/nano-structures are manufactured for applications in 3D data storage and optical information encryption.This research presents an effective strategy for the fabrication of desired QD micro-/nano-structures,supporting the development of stable functional device applications.展开更多
Superconducting kinetic inductance detectors(KIDs)are considered to be a highly promising technique for the large-scale imaging of millimeter and submillimeter waves in astronomy.As the pixel density and the array siz...Superconducting kinetic inductance detectors(KIDs)are considered to be a highly promising technique for the large-scale imaging of millimeter and submillimeter waves in astronomy.As the pixel density and the array size increase,the electromagnetic crosstalk inevitably becomes a problem that prevents increasing the multiplexing during the development of larger KIDs arrays.In this work,an effective method is introduced to suppress the electromagnetic crosstalk and achieve a compact pixel distribution and small frequency intervals.The electromagnetic crosstalk is first analyzed by simulating the behavior of two neighboring pixels,and the physical distance and the frequency interval are optimized.Then,the arrangement of the pixels on the whole array is redesigned using a genetic algorithm to satisfy the requirements.The simulation results reveal that the normalized electromagnetic crosstalk can be reduced to 0.5%on an 8×8 array.Larger arrays of 16×16 pixels have been fabricated and measured to validate this method,and the results reveal that both the resonance property and survival rate of pixels are improved effectively with this method.This method will be very helpful for designing high-multiplexing KIDs arrays within a limited bandwidth.展开更多
To accurately predict coal burst hazards and estimate the failure of coal pillars in underground coal mining systems,it is of great significance to understand the mechanical behavior of coal-rock bimaterial composite ...To accurately predict coal burst hazards and estimate the failure of coal pillars in underground coal mining systems,it is of great significance to understand the mechanical behavior of coal-rock bimaterial composite structures.This paper presents experimental and numerical investigations on the response of rock-coal,coal-rock,and rock-coal-rock bimaterial composite structures under triaxial compression.The triaxial compression experiments are conducted under confining pressures in the range of 0-20 MPa.The resulting inside fracture networks are detected using X-ray-based computed tomography(CT).The experimentally observed data indicate that the mechanical parameters of the rock-coalrock composites are superior to those of the rock-coal and coal-rock combinations.After compression failure,the coal-rock combination specimens are analyzed via X-ray CT.The results display that the failure of the coal-rock composite bodies primarily takes place within the coal.Further,the bursting proneness is reduced by increasing confining pressure.Subsequently,the corresponding numerical simulations of the experiments are carried out by using the particle flow code.The numerical results reveal that coal is vulnerable with regard to energy storage and accumulation.展开更多
In a strong crosswind,the wake of a bridge tower will lead to an abrupt change of the aerodynamic forces acting on a vehicle passing through it,which may result in problems related to the transportation safety.This st...In a strong crosswind,the wake of a bridge tower will lead to an abrupt change of the aerodynamic forces acting on a vehicle passing through it,which may result in problems related to the transportation safety.This study investigates the transient aerodynamic characteristics of a high-speed train moving in a truss girder bridge and passing by a bridge tower in a wind tunnel.The scaled ratio of the train,bridge,and tower are 1:30.Effects of various parameters such as the incoming wind speed,train speed,and yaw angle on the aerodynamic performance of the train were considered.Then the sudden change mechanism of aerodynamic loads on the train when it crosses over the tower was further discussed.The results show that the bridge tower has an apparent shielding effect on the train passing through it,with the influencing width being larger than the width of the tower.The train speed is the main factor affecting the influencing width of aerodynamic coefficients,and the mutation amplitude is mainly related to the yaw angle obtained by changing the incoming wind speed or train speed.The vehicle movement introduces an asymmetry of loading on the train in the process of approaching and leaving the wake of the bridge tower,which should not be neglected.展开更多
This paper experimentally studied the stress corrosion behavior of 2195-T8 Al-Li alloys with prefabricated pits in 30vol%HNO3.The microstructure was observed and determined by the scanning electron microscopy and the ...This paper experimentally studied the stress corrosion behavior of 2195-T8 Al-Li alloys with prefabricated pits in 30vol%HNO3.The microstructure was observed and determined by the scanning electron microscopy and the transmission electron microscopy.The typical corrosion features,i.e.,regular or irregular corrosion pits and sub/intergranular corrosion,were investigated,and the role of stress were analyzed.The statistical results of the number and area of corrosion pits in various sizes were studied using an improved image processing method.Experimental results demonstrate that stress affects the intergranular corrosion or sub-intergranular corrosion of the alloy most,and promotes them to evolve into cracks under stress corrosion.Stress also plays a role in accelerating corrosion severity of certain corrosion feature.The number of corrosion pits with an area of 0–50μm~2 can be seen as an index to reflect the degree of corrosion.Besides,a corrosion phenomenon,i.e.,the pit bottom in the site with the maximum stress locally suffering from no severe corrosion,was observed and discussed.展开更多
Optical fibers are typically used in telecommunications services for data transmission,where the use of fiber tags is essential to distinguish between the different transmission fibers or channels and thus ensure the ...Optical fibers are typically used in telecommunications services for data transmission,where the use of fiber tags is essential to distinguish between the different transmission fibers or channels and thus ensure the working functionality of the communication system.Traditional physical entity marking methods for fiber labeling are bulky,easily confused,and,most importantly,the label information can be accessed easily by all potential users.This work proposes an encrypted optical fiber tag based on an encoded fiber Bragg grating(FBG)array that is fabricated using a point-by-point femtosecond laser pulse chain inscription method.Gratings with different resonant wavelengths and reflectivities are realized by adjusting the grating period and the refractive index modulations.It is demonstrated that a binary data sequence carried by a fiber tag can be inscribed into the fiber core in the form of an FBG array,and the tag data can be encrypted through appropriate design of the spatial distributions of the FBGs with various reflection wavelengths and reflectivities.The proposed fiber tag technology can be used for applications in port identification,encrypted data storage,and transmission in fiber networks.展开更多
Campylobacter spp. are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of Campylobacter spp. (C. spp.) that confers high-level resistanc...Campylobacter spp. are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of Campylobacter spp. (C. spp.) that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines (TETs), all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P < 0.05), but not for ciprofloxacin (CIP), TET, and gentamicin (GEN). However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.展开更多
The emergence and spread of the mobile colistin-resistance gene,mcr-1,and its variants pose achallenge to the use of colistin,a last-resort antibiotic used to treat severe infections caused by extensively drug-resista...The emergence and spread of the mobile colistin-resistance gene,mcr-1,and its variants pose achallenge to the use of colistin,a last-resort antibiotic used to treat severe infections caused by extensively drug-resistant(XDR)Gram-negative pathogens.Antibiotic adjuvants are a promising strategy to enhance the efficacy of colistin against colistin-resistant pathogens;however,few studies have considered the effects of adjuvants on limiting resistance-gene transmission.We found that chelerythrine(4 mg·L^(-1))derived from Macleaya cordata extract,which is used as an animal feed additive,reduced the minimal inhibitory concentration(MIC)of colistin against an mcr-1 positive Escherichia coli(E.coli)strain by 16-fold(from 2.000 to 0.125 mg·L^(-1)).eliminated approximately 10^(4) colony-forming units(CFUs)of an mcr-1-carrying strain in a murine intestinal infection model,and inhibited the conjugation of an mcr-1-bearing plasmid in vitro(by>100-fold)and in a mouse model(by up to 5-fold).A detailed analysis revealed that chelery-thrine binds to phospholipids on bacterial membranes and increases cytoplasmic membrane fluidity,thereby impairing respiration,disrupting proton motive force(PMF),generating reactive oxygen species(ROS),and decreasing intracellular adenosine triphosphate(ATP)levels,which subsequently downregu-lates mcr-1 and conjugation-associated genes.These dual effects of chelerythrine can expand the use of antibiotic adjuvants and may provide a new strategy for circumventing mobile colistin resistance.展开更多
The low-velocity impact response and infrared radiation characteristics of composites have rarely been focused on simultaneously.This study aims to investigate the low-velocity impact response and infrared radiation c...The low-velocity impact response and infrared radiation characteristics of composites have rarely been focused on simultaneously.This study aims to investigate the low-velocity impact response and infrared radiation characteristics of the glass fiber reinforced thermoplastic polypropylene and carbon fiber reinforced thermosetting epoxy resin laminates wildly used in the aircraft industry.The impact tests were conducted at five energy levels.Characterization parameters such as impact load,displacement,and absorbed energy were measured.The damage evolution and damage modes of the laminates were analyzed through active and passive thermography,ultrasonic C-scan,and optical microscope.The results indicate that Thermosets(TS)laminates exhibit better impact resistance,while Thermoplastics(TP)laminates show higher delamination ductility,and the maximum contact force of TP laminates is much smaller than that of the TS laminates under lowvelocity impacts,but the low bending stiffness and low ductility of the TP matrix cause the difference in energy absorption level between the two not significant.The temperature characteristic changes of passive infrared thermography heat maps could characterize the damage mode of the laminates.The correlation between the heat maps and the impact characteristic curves is explained;the fluctuation of the impact characteristic curves is directly related to the hot spot characteristics changes of the heat maps.More frequent curve fluctuations correspond to a larger and brighter hot spot on the heat map,which peaks at the maximum impact load after the impact force versus time curve fluctuation cutoff point,the maximum center displacement of the impact force versus displacement curve,and the maximum absorbed energy of the absorbed energy versus time curve.展开更多
Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At p...Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At present,the impact of livestock-associated CREC strains on human populations at the national level is unknown.Here,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence.We also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin.Overall,631/29799(2.1%)clinical Escherichia coli(E.coli)isolates were identified as CREC.Multivariable analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC infection.In general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),respectively.Among all the Chinese NDM-positive E.coli(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels.All the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe.展开更多
What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healt...What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healthcare settings.What is added by this report?A comprehensive surveillance study revealed enterococci in 14.39%of clinical samples from dogs and cats across China during 2018–2021.Multidrugresistant enterococcal infections showed significant correlation with urinary tract catheterization and extended hospitalization periods.Notably,pet-derived Enterococcus faecalis isolates demonstrated high genetic similarity with strains isolated from humans,farm animals,and environmental sources.What are the implications for public health practice?These findings underscore the critical need for enhanced surveillance of enterococcal infections and implementation of stringent aseptic protocols in veterinary clinical settings.Particular attention should be directed toward linezolid-resistant Enterococcus faecalis infections due to their demonstrated potential for transmission between pets and humans.展开更多
Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus hav...Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus have exhibited considerable potentials in the fields of microforce,micro-vibration,and microforce sensors.In this work,a controllable microstructured cantilever probe on an optical fiber tip for microforce detection is demonstrated both theoretically and experimentally.The static performances of the probe are firstly investigated based on the finite element method(FEM),which provides the basis for the structural design.The proposed cantilever probe is then 3D printed by means of the TPP technology.The experimental results show that the elastic constant k of the proposed cantilever probe can be actively tuned from 2.46N/m to 62.35N/m.The force sensitivity is 2.5nm/μN,the Q-factor is 368.93,and the detection limit is 57.43nN.Moreover,the mechanical properties of the cantilever probe can be flexibly adjusted by the geometric configuration of the cantilever.Thus,it has an enormous potential for matching the mechanical properties of biological samples in the direct contact mode.展开更多
A novel,highly efficient and accurate adaptive higher-order finite element method(hp-FEM)is used to simulate a multi-frequency resistivity loggingwhile-drilling(LWD)tool response in a borehole environment.Presented in...A novel,highly efficient and accurate adaptive higher-order finite element method(hp-FEM)is used to simulate a multi-frequency resistivity loggingwhile-drilling(LWD)tool response in a borehole environment.Presented in this study are the vector expression of Maxwell’s equations,three kinds of boundary conditions,stability weak formulation of Maxwell’s equations,and automatic hpadaptivity strategy.The new hp-FEM can select optimal refinement and calculation strategies based on the practical formation model and error estimation.Numerical experiments show that the new hp-FEM has an exponential convergence rate in terms of relative error in a user-prescribed quantity of interest against the degrees of freedom,which provides more accurate results than those obtained using the adaptive h-FEM.The numerical results illustrate the high efficiency and accuracy of the method at a given LWD tool structure and parameters in different physical models,which further confirm the accuracy of the results using the Hermes library(http://hpfem.org/hermes)with a multi-frequency resistivity LWD tool response in a borehole environment.展开更多
As of this writing,the world is currently experiencing a huge wave of infection with the Omicron variant of SARS-CoV-2.Two SARS-CoV-2inactivated vaccines(IAVs,CoronaVac by Sinovac and BBIBP-CorV by Sinopharm)with a tw...As of this writing,the world is currently experiencing a huge wave of infection with the Omicron variant of SARS-CoV-2.Two SARS-CoV-2inactivated vaccines(IAVs,CoronaVac by Sinovac and BBIBP-CorV by Sinopharm)with a two-dose vaccination regimen,one recombinant protein subunit vaccine(PRV,ZF2001 by Anhui Zhifei Longcom)with a three-dose vaccination regimen,and one single-dose recombinant adenovirus-vectored vaccine(AdV.Convidecia by Cansino)have been given conditional approval for general public use or approved for emergency use by China[1].These four vaccines form the core of China's vaccination program.It has been reported that Omicron extensively escapes vaccine neutralization,and a booster shot seems to be necessary[2.3].It is urgent to discover a superior booster strategy in China's current vaccination program context to fight against the Omicron variant.展开更多
The emergence and worldwide dissemination of mobile tigecycline resistance genes tet(X3)/tet(X4)posed an enor-mous threat to the public health.Urgently,feasible strategies must be implemented to restore the clinical e...The emergence and worldwide dissemination of mobile tigecycline resistance genes tet(X3)/tet(X4)posed an enor-mous threat to the public health.Urgently,feasible strategies must be implemented to restore the clinical efficacy of tetracyclines and prolong the lifespan of existing drugs to address the emerging global antimicrobial resistance threat.Herein,versatile structural scaffolds of quinones for antibiotic adjuvants discovery enlightened a promising and underappreciated reservoir to circumvent the antibiotic resistance.2-methoxy-1,4-naphthoquinone(MNQ)exhib-ited the potent potentiation(4 to 32-fold)with tetracyclines,along with effective inhibition on biofilm formation.Mechanistic studies revealed that MNQ synergistically operates with tetracyclines by inhibiting the enzymatic activity of Tet(X3)/Tet(X4)proteins through interaction with their active residues.Furthermore,exposure to MNQ significantly dissipate the proton motive force,leading to a cascade of membrane structural damage and metabolic homeostasis imbalance.Encouragingly,the MNQ-tetracyclines combination showcased substantial therapeutic benefits in two in vivo infection models,as evidenced by the reduced bacterial burden and mitigated pathological injury.Our find-ings propose a potential therapeutic option and a novel tetracyclines’adjuvant against drug-resistant pathogens carrying Tet(X3)/Tet(X4).展开更多
In this study,we propose the use of a fiber-reinforced plastic grid with polymer−cement−mortar(FRP Grid-PCM)to reinforce segment joints in tunnel shield linings.These joints play a crucial role in determining bearing ...In this study,we propose the use of a fiber-reinforced plastic grid with polymer−cement−mortar(FRP Grid-PCM)to reinforce segment joints in tunnel shield linings.These joints play a crucial role in determining bearing capacity but are vulnerable to deterioration during operation.To investigate how to enhance the flexural performance of longitudinal shield lining joints,we built eccentric short column specimens by bolting two half-corbel columns together and tested them in the laboratory.The test program comprised two control specimens and three strengthened specimens with FRP grid applied on one side,away from the axial load.The tests varied two main parameters:loading eccentricity and the number of FRP grid layers.We conducted a detailed analysis of the failure process,bearing capacity,and bending stiffness of longitudinal joints under different conditions.Furthermore,we developed an analytical model to predict the flexural bearing capacity of longitudinal joints upgraded with the FRP Grid-PCM method and validated it through experimental results.The research demonstrates that the FRP grid effectively reduces joint opening and rotation angles while enhancing the bearing capacity of the short column,particularly with concurrent increases in loading eccentricity and the number of FRP grid layers.Overall,our findings offer a novel alternative for improving the flexural performance of longitudinal joints in shield tunnels.展开更多
The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to...The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to inves-tigate the epidemic features and transmission dynamics of tet(X4)-positive Klebsiella pneumoniae(K.pneumo-niae)through the analysis of 206 raw meats,including pork(n=182),beef(n=16),duck(n=5),and chicken(n=3).These samples were collected from schools,markets,and restaurants in Chengdu City,China.A total of 25 isolates were obtained from 13 administrative regions.All isolates exhibited resistance to tetracycline,tigecycline,ampicillin,chloramphenicol,and florfenicol.Over half of the isolates also demon-strated resistance to streptomycin(80%),sulfamethoxazole/trimethoprim(72%),ciprofloxacin(64%),and ampicillin/sulbactam(56%).Among these strains,14 distinct sequence types(STs)were identified,revealing evidence of inter-regional clonal spread,notably among 9 K.pneumoniae ST3393.Phylogenetic analysis revealed the presence of two K.pneumoniae ST5 closely resembling hypervirulent K.pneumoniae from Jiangsu.Importantly,12 isolates were capable of transferring tigecycline resistance to Escherichia coli J53.Further plasmid analysis showed that the tet(X4)-harboring plasmids in K.pneumoniae could be classified into four types,primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid(n=16)and IncFII plasmid(n=7),which significantly contributed to the cross-species dissemination of tet(X4).In summary,this study highlights the prevalence of MDR tet(X4)-positive K.pneumoniae in Chengdu,driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer.These findings emphasize the importance of contin-uous surveillance of tet(X4)-positive K.pneumoniae in raw meat and the implementation of effective measures to control their spread.展开更多
This study aimed to investigate the protective effect of the nature product osthole(OST)against Clostridium perfrin-gens type A infection-caused myonecrosis in a mouse model.Male mice were divided into(1)control,(2)in...This study aimed to investigate the protective effect of the nature product osthole(OST)against Clostridium perfrin-gens type A infection-caused myonecrosis in a mouse model.Male mice were divided into(1)control,(2)infected,(3)OST50 and(4)OST100 treatment groups.In the infected groups,mice were intramuscularly injected with 1×10^(8) CFU of C.perfringens per day for 6 days.Mice in the OST50 and OST100 groups were administrated intraperitoneally with OST at the doses of 50 or 100 mg/kg per day post C.perfringens infection.Our results showed that C.perfringens infection caused marked necrosis and inflammatory cell infiltration in the muscle tissues of mice.Mice in the OST50 and OST100 treatment groups displayed significantly attenuated C.perfringens infection-induced lipid peroxida-tion,oxidative stress,and apoptosis in their muscle tissue.Furthermore,OST treatment significantly downregulated the expressions of NF-κB,IL-1β,and TNF-αmRNA and protein levels,while concomitantly upregulating the expressions of Nrf2 and HO-1 mRNA and protein.OST treatments also inhibited the expression of phosphorylation(p)-p38,p-mTOR,and p-Erk1/2 proteins,and upregulated LC3II and Beclin1 proteins.In summary,our results reveal that OST therapy confers a protective effect against C.perfringens infection-induced oxidative stress and inflammation in muscle tissue,via activation of Nrf2/HO-1 and autophagy pathways and inhibition of p38,Erk1/2 and NF-κB pathways.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.11802332)the China Scholarship Council(Grant No.202206435003)the Fundamental Research Funds for the Central Universities(Grant No.2024ZKPYLJ03).
文摘The internal microstructures of rock materials, including mineral heterogeneity and intrinsic microdefects, exert a significant influence on their nonlinear mechanical and cracking behaviors. It is of great significance to accurately characterize the actual microstructures and their influence on stress and damage evolution inside the rocks. In this study, an image-based fast Fourier transform (FFT) method is developed for reconstructing the actual rock microstructures by combining it with the digital image processing (DIP) technique. A series of experimental investigations were conducted to acquire information regarding the actual microstructure and the mechanical properties. Based on these experimental evidences, the processed microstructure information, in conjunction with the proposed micromechanical model, is incorporated into the numerical calculation. The proposed image-based FFT method was firstly validated through uniaxial compression tests. Subsequently, it was employed to predict and analyze the influence of microstructure on macroscopic mechanical behaviors, local stress distribution and the internal crack evolution process in brittle rocks. The distribution of feldspar is considerably more heterogeneous and scattered than that of quartz, which results in a greater propensity for the formation of cracks in feldspar. It is observed that initial cracks and new cracks, including intragranular and boundary ones, ultimately coalesce and connect as the primary through cracks, which are predominantly distributed along the boundary of the feldspar. This phenomenon is also predicted by the proposed numerical method. The results indicate that the proposed numerical method provides an effective approach for analyzing, understanding and predicting the nonlinear mechanical and cracking behaviors of brittle rocks by taking into account the actual microstructure characteristics.
基金supported by the National Key Research and Development Program of China(2024YFB3213700)Shenzhen Science and Technology Program(Shenzhen Key Laboratory of Ultrafast Laser Micro/Nano Manufacturing ZDSYS20220606100405013)+3 种基金Natural Science Foundation of Guangdong Province(2022B1515120061)National Natural Science Foundation of China(T2421003)Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20231722)Research Team Cultivation Program of Shenzhen University(2023QNT009)。
文摘Semiconductor quantum dots(QDs),as high-performance materials,play an essential role in contemporary industry,mainly due to their high photoluminescent quantum yield,wide absorption characteristics,and size-dependent light emission.It is essential to construct well-defined micro-/nano-structures using QDs as building blocks for micro-optic applications.However,the fabrication of stable QDs with designed functional structures has long been challenging.Here,we propose a strategy for three-dimensional direct lithography of desired QDs within a hybrid medium with specific protection properties.The acrylate-functionalized hybrid precursors enable local crosslinking through ultrafast laser-induced multiphoton absorption,achieving sub-100 nm resolution surpassing the diffraction limit.The printed micro-/nano-structures possess thermal stability up to 600℃,which can be transformed to inorganic architectures with a volume shrinkage.Due to the encapsulated QDs within the densely silicon-oxygen molecular networks,the functional structures demonstrate good stability against ultraviolet irradiation,corrosive solutions,and elevated temperatures.Based on hybrid3D nanolithography,bicolor multilayer micro-/nano-structures are manufactured for applications in 3D data storage and optical information encryption.This research presents an effective strategy for the fabrication of desired QD micro-/nano-structures,supporting the development of stable functional device applications.
基金supported by the National Key Research and Development Program of China(2023YFC2206601)the National Natural Science Foundation of China(12273024,62205211)the Science and Technology Commission of Shanghai Municipality(23010503900,22590780100).
文摘Superconducting kinetic inductance detectors(KIDs)are considered to be a highly promising technique for the large-scale imaging of millimeter and submillimeter waves in astronomy.As the pixel density and the array size increase,the electromagnetic crosstalk inevitably becomes a problem that prevents increasing the multiplexing during the development of larger KIDs arrays.In this work,an effective method is introduced to suppress the electromagnetic crosstalk and achieve a compact pixel distribution and small frequency intervals.The electromagnetic crosstalk is first analyzed by simulating the behavior of two neighboring pixels,and the physical distance and the frequency interval are optimized.Then,the arrangement of the pixels on the whole array is redesigned using a genetic algorithm to satisfy the requirements.The simulation results reveal that the normalized electromagnetic crosstalk can be reduced to 0.5%on an 8×8 array.Larger arrays of 16×16 pixels have been fabricated and measured to validate this method,and the results reveal that both the resonance property and survival rate of pixels are improved effectively with this method.This method will be very helpful for designing high-multiplexing KIDs arrays within a limited bandwidth.
基金This study was financially supported by Beijing Outstanding Young Scientist Program(BJJWZYJH01201911413037)National Natural Science Foundation of China(41877257+3 种基金5162240452009131)Shaanxi Coal Group Key Project(2018SMHKJ-A-J-03)Yueqi outstanding scholar Award Program by CUMTB。
文摘To accurately predict coal burst hazards and estimate the failure of coal pillars in underground coal mining systems,it is of great significance to understand the mechanical behavior of coal-rock bimaterial composite structures.This paper presents experimental and numerical investigations on the response of rock-coal,coal-rock,and rock-coal-rock bimaterial composite structures under triaxial compression.The triaxial compression experiments are conducted under confining pressures in the range of 0-20 MPa.The resulting inside fracture networks are detected using X-ray-based computed tomography(CT).The experimentally observed data indicate that the mechanical parameters of the rock-coalrock composites are superior to those of the rock-coal and coal-rock combinations.After compression failure,the coal-rock combination specimens are analyzed via X-ray CT.The results display that the failure of the coal-rock composite bodies primarily takes place within the coal.Further,the bursting proneness is reduced by increasing confining pressure.Subsequently,the corresponding numerical simulations of the experiments are carried out by using the particle flow code.The numerical results reveal that coal is vulnerable with regard to energy storage and accumulation.
基金The authors would like to gratefully acknowledge the supports from the National Natural Science Foundation of China(No.U1434205,51708645)Zhejiang Provincial Natural Science Foundation of China(No.LY19E080016).
文摘In a strong crosswind,the wake of a bridge tower will lead to an abrupt change of the aerodynamic forces acting on a vehicle passing through it,which may result in problems related to the transportation safety.This study investigates the transient aerodynamic characteristics of a high-speed train moving in a truss girder bridge and passing by a bridge tower in a wind tunnel.The scaled ratio of the train,bridge,and tower are 1:30.Effects of various parameters such as the incoming wind speed,train speed,and yaw angle on the aerodynamic performance of the train were considered.Then the sudden change mechanism of aerodynamic loads on the train when it crosses over the tower was further discussed.The results show that the bridge tower has an apparent shielding effect on the train passing through it,with the influencing width being larger than the width of the tower.The train speed is the main factor affecting the influencing width of aerodynamic coefficients,and the mutation amplitude is mainly related to the yaw angle obtained by changing the incoming wind speed or train speed.The vehicle movement introduces an asymmetry of loading on the train in the process of approaching and leaving the wake of the bridge tower,which should not be neglected.
基金supported by the National Natural Science Foundation of China(No.52075541)the Natural Science Foundation of Shaanxi Province,China(No.2020JM-354)。
文摘This paper experimentally studied the stress corrosion behavior of 2195-T8 Al-Li alloys with prefabricated pits in 30vol%HNO3.The microstructure was observed and determined by the scanning electron microscopy and the transmission electron microscopy.The typical corrosion features,i.e.,regular or irregular corrosion pits and sub/intergranular corrosion,were investigated,and the role of stress were analyzed.The statistical results of the number and area of corrosion pits in various sizes were studied using an improved image processing method.Experimental results demonstrate that stress affects the intergranular corrosion or sub-intergranular corrosion of the alloy most,and promotes them to evolve into cracks under stress corrosion.Stress also plays a role in accelerating corrosion severity of certain corrosion feature.The number of corrosion pits with an area of 0–50μm~2 can be seen as an index to reflect the degree of corrosion.Besides,a corrosion phenomenon,i.e.,the pit bottom in the site with the maximum stress locally suffering from no severe corrosion,was observed and discussed.
基金supported by the National Natural Science Foundation of China(62122057,62075136,62105217,62205221,62205222)the Basic and Applied Basic Research Foundation of Guangdong Province(2022B1515120061)Shenzhen Science and Technology Program(Shenzhen Key Laboratory of Ultrafast Laser Micro/Nano Manufacturing ZDSYS20220606100405013,RCYX20200714114524139,JCYJ20200109114001806)。
文摘Optical fibers are typically used in telecommunications services for data transmission,where the use of fiber tags is essential to distinguish between the different transmission fibers or channels and thus ensure the working functionality of the communication system.Traditional physical entity marking methods for fiber labeling are bulky,easily confused,and,most importantly,the label information can be accessed easily by all potential users.This work proposes an encrypted optical fiber tag based on an encoded fiber Bragg grating(FBG)array that is fabricated using a point-by-point femtosecond laser pulse chain inscription method.Gratings with different resonant wavelengths and reflectivities are realized by adjusting the grating period and the refractive index modulations.It is demonstrated that a binary data sequence carried by a fiber tag can be inscribed into the fiber core in the form of an FBG array,and the tag data can be encrypted through appropriate design of the spatial distributions of the FBGs with various reflection wavelengths and reflectivities.The proposed fiber tag technology can be used for applications in port identification,encrypted data storage,and transmission in fiber networks.
文摘Campylobacter spp. are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of Campylobacter spp. (C. spp.) that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines (TETs), all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P < 0.05), but not for ciprofloxacin (CIP), TET, and gentamicin (GEN). However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.
基金grants from the Laboratory of Lingnan Modern Agriculture Project(NT2021006 to Yang Wang and Jianzhong Shen)the National Natural Science Foundation of China(81861138051 and 81991535 to Yang Wang and Congming Wu).
文摘The emergence and spread of the mobile colistin-resistance gene,mcr-1,and its variants pose achallenge to the use of colistin,a last-resort antibiotic used to treat severe infections caused by extensively drug-resistant(XDR)Gram-negative pathogens.Antibiotic adjuvants are a promising strategy to enhance the efficacy of colistin against colistin-resistant pathogens;however,few studies have considered the effects of adjuvants on limiting resistance-gene transmission.We found that chelerythrine(4 mg·L^(-1))derived from Macleaya cordata extract,which is used as an animal feed additive,reduced the minimal inhibitory concentration(MIC)of colistin against an mcr-1 positive Escherichia coli(E.coli)strain by 16-fold(from 2.000 to 0.125 mg·L^(-1)).eliminated approximately 10^(4) colony-forming units(CFUs)of an mcr-1-carrying strain in a murine intestinal infection model,and inhibited the conjugation of an mcr-1-bearing plasmid in vitro(by>100-fold)and in a mouse model(by up to 5-fold).A detailed analysis revealed that chelery-thrine binds to phospholipids on bacterial membranes and increases cytoplasmic membrane fluidity,thereby impairing respiration,disrupting proton motive force(PMF),generating reactive oxygen species(ROS),and decreasing intracellular adenosine triphosphate(ATP)levels,which subsequently downregu-lates mcr-1 and conjugation-associated genes.These dual effects of chelerythrine can expand the use of antibiotic adjuvants and may provide a new strategy for circumventing mobile colistin resistance.
基金the Major Research Plan of the National Natural Science Foundation of China (No. 92060106)the National Natural Science Foundation of China (No. 52075541)+1 种基金the China Postdoctoral Science Foundation (No. 2019M650262)the Natural Science Foundation of Shaanxi Province, China (No. 2020JM-354)
文摘The low-velocity impact response and infrared radiation characteristics of composites have rarely been focused on simultaneously.This study aims to investigate the low-velocity impact response and infrared radiation characteristics of the glass fiber reinforced thermoplastic polypropylene and carbon fiber reinforced thermosetting epoxy resin laminates wildly used in the aircraft industry.The impact tests were conducted at five energy levels.Characterization parameters such as impact load,displacement,and absorbed energy were measured.The damage evolution and damage modes of the laminates were analyzed through active and passive thermography,ultrasonic C-scan,and optical microscope.The results indicate that Thermosets(TS)laminates exhibit better impact resistance,while Thermoplastics(TP)laminates show higher delamination ductility,and the maximum contact force of TP laminates is much smaller than that of the TS laminates under lowvelocity impacts,but the low bending stiffness and low ductility of the TP matrix cause the difference in energy absorption level between the two not significant.The temperature characteristic changes of passive infrared thermography heat maps could characterize the damage mode of the laminates.The correlation between the heat maps and the impact characteristic curves is explained;the fluctuation of the impact characteristic curves is directly related to the hot spot characteristics changes of the heat maps.More frequent curve fluctuations correspond to a larger and brighter hot spot on the heat map,which peaks at the maximum impact load after the impact force versus time curve fluctuation cutoff point,the maximum center displacement of the impact force versus displacement curve,and the maximum absorbed energy of the absorbed energy versus time curve.
基金supported in part by the grants from National Natural Science Foundation of China(81991535,81861138051,and 81871690)UK MRC DETER-XDR-China-HUB(MR/S013768/1)。
文摘Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At present,the impact of livestock-associated CREC strains on human populations at the national level is unknown.Here,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence.We also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin.Overall,631/29799(2.1%)clinical Escherichia coli(E.coli)isolates were identified as CREC.Multivariable analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC infection.In general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),respectively.Among all the Chinese NDM-positive E.coli(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels.All the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe.
基金Supported by the National Key Research and Development Program of China(2022YFD1800400)National Natural Science Foundation of China(81991531,32002339)2115 Talent Development Program of China Agricultural University.
文摘What is already known about this topic?Enterococcus spp.,while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans,have emerged as significant opportunistic pathogens in healthcare settings.What is added by this report?A comprehensive surveillance study revealed enterococci in 14.39%of clinical samples from dogs and cats across China during 2018–2021.Multidrugresistant enterococcal infections showed significant correlation with urinary tract catheterization and extended hospitalization periods.Notably,pet-derived Enterococcus faecalis isolates demonstrated high genetic similarity with strains isolated from humans,farm animals,and environmental sources.What are the implications for public health practice?These findings underscore the critical need for enhanced surveillance of enterococcal infections and implementation of stringent aseptic protocols in veterinary clinical settings.Particular attention should be directed toward linezolid-resistant Enterococcus faecalis infections due to their demonstrated potential for transmission between pets and humans.
基金supported by the Shenzhen Science and Technology Program (Grant No.RCYX20200714114524139)Shenzhen Key Laboratory of Ultrafast Laser Micro/Nano Manufacturing (Grant No.ZDSYS20220606100405013)+2 种基金Natural Science Foundation of GuangdongProvince (Grant Nos.2022B1515120061 and 2022A1515110971)National Natural Science Foundation of China (Grant Nos.62122057,62075136,62105217,and 62305223)China Postdoctoral Science Foundation (Grant No.2022M722173).
文摘Benefiting from the great advances of the femtosecond laser two-photon polymerization(TPP)technology,customized microcantilever probes can be accurately 3-dimensional(3D)manufactured at the nanoscale size and thus have exhibited considerable potentials in the fields of microforce,micro-vibration,and microforce sensors.In this work,a controllable microstructured cantilever probe on an optical fiber tip for microforce detection is demonstrated both theoretically and experimentally.The static performances of the probe are firstly investigated based on the finite element method(FEM),which provides the basis for the structural design.The proposed cantilever probe is then 3D printed by means of the TPP technology.The experimental results show that the elastic constant k of the proposed cantilever probe can be actively tuned from 2.46N/m to 62.35N/m.The force sensitivity is 2.5nm/μN,the Q-factor is 368.93,and the detection limit is 57.43nN.Moreover,the mechanical properties of the cantilever probe can be flexibly adjusted by the geometric configuration of the cantilever.Thus,it has an enormous potential for matching the mechanical properties of biological samples in the direct contact mode.
基金The work for this paper was supported by the National Natural Science Foundation of China under Projects No.41074099。
文摘A novel,highly efficient and accurate adaptive higher-order finite element method(hp-FEM)is used to simulate a multi-frequency resistivity loggingwhile-drilling(LWD)tool response in a borehole environment.Presented in this study are the vector expression of Maxwell’s equations,three kinds of boundary conditions,stability weak formulation of Maxwell’s equations,and automatic hpadaptivity strategy.The new hp-FEM can select optimal refinement and calculation strategies based on the practical formation model and error estimation.Numerical experiments show that the new hp-FEM has an exponential convergence rate in terms of relative error in a user-prescribed quantity of interest against the degrees of freedom,which provides more accurate results than those obtained using the adaptive h-FEM.The numerical results illustrate the high efficiency and accuracy of the method at a given LWD tool structure and parameters in different physical models,which further confirm the accuracy of the results using the Hermes library(http://hpfem.org/hermes)with a multi-frequency resistivity LWD tool response in a borehole environment.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29040201)the National Natural Science Foundation of China(NSFC)(81901680)。
文摘As of this writing,the world is currently experiencing a huge wave of infection with the Omicron variant of SARS-CoV-2.Two SARS-CoV-2inactivated vaccines(IAVs,CoronaVac by Sinovac and BBIBP-CorV by Sinopharm)with a two-dose vaccination regimen,one recombinant protein subunit vaccine(PRV,ZF2001 by Anhui Zhifei Longcom)with a three-dose vaccination regimen,and one single-dose recombinant adenovirus-vectored vaccine(AdV.Convidecia by Cansino)have been given conditional approval for general public use or approved for emergency use by China[1].These four vaccines form the core of China's vaccination program.It has been reported that Omicron extensively escapes vaccine neutralization,and a booster shot seems to be necessary[2.3].It is urgent to discover a superior booster strategy in China's current vaccination program context to fight against the Omicron variant.
基金supported by the National Natural Science Foundation of China(grant No.U22A20523,32202856,32172912 and 32102723)the Postdoctoral Research Foundation of China(Certificate Number:2023 M731291)+1 种基金Interdisciplinary Integration and Innovation Project of JLU(JLUXKJC2021QZ04)the Graduate Innovation Fund of Jilin University(No.2022001).
文摘The emergence and worldwide dissemination of mobile tigecycline resistance genes tet(X3)/tet(X4)posed an enor-mous threat to the public health.Urgently,feasible strategies must be implemented to restore the clinical efficacy of tetracyclines and prolong the lifespan of existing drugs to address the emerging global antimicrobial resistance threat.Herein,versatile structural scaffolds of quinones for antibiotic adjuvants discovery enlightened a promising and underappreciated reservoir to circumvent the antibiotic resistance.2-methoxy-1,4-naphthoquinone(MNQ)exhib-ited the potent potentiation(4 to 32-fold)with tetracyclines,along with effective inhibition on biofilm formation.Mechanistic studies revealed that MNQ synergistically operates with tetracyclines by inhibiting the enzymatic activity of Tet(X3)/Tet(X4)proteins through interaction with their active residues.Furthermore,exposure to MNQ significantly dissipate the proton motive force,leading to a cascade of membrane structural damage and metabolic homeostasis imbalance.Encouragingly,the MNQ-tetracyclines combination showcased substantial therapeutic benefits in two in vivo infection models,as evidenced by the reduced bacterial burden and mitigated pathological injury.Our find-ings propose a potential therapeutic option and a novel tetracyclines’adjuvant against drug-resistant pathogens carrying Tet(X3)/Tet(X4).
基金supported by the National Key R&D Program of China(No.2023YFB2390300)the National Natural Science Foundation of China(Nos.52379102 and 51878658)+1 种基金Joint Fund of State Key Laboratory of Coal Resources and Safe Mining-the Beijing Outstanding Young Scientist Program(Nos.SKLCRSM20LH03 and BJJWZYJH01201911413037)The Fundamental Research Funds for the Central Universities(No.2024ZKPYLJ03).
文摘In this study,we propose the use of a fiber-reinforced plastic grid with polymer−cement−mortar(FRP Grid-PCM)to reinforce segment joints in tunnel shield linings.These joints play a crucial role in determining bearing capacity but are vulnerable to deterioration during operation.To investigate how to enhance the flexural performance of longitudinal shield lining joints,we built eccentric short column specimens by bolting two half-corbel columns together and tested them in the laboratory.The test program comprised two control specimens and three strengthened specimens with FRP grid applied on one side,away from the axial load.The tests varied two main parameters:loading eccentricity and the number of FRP grid layers.We conducted a detailed analysis of the failure process,bearing capacity,and bending stiffness of longitudinal joints under different conditions.Furthermore,we developed an analytical model to predict the flexural bearing capacity of longitudinal joints upgraded with the FRP Grid-PCM method and validated it through experimental results.The research demonstrates that the FRP grid effectively reduces joint opening and rotation angles while enhancing the bearing capacity of the short column,particularly with concurrent increases in loading eccentricity and the number of FRP grid layers.Overall,our findings offer a novel alternative for improving the flexural performance of longitudinal joints in shield tunnels.
基金supported by the National Key Research and Development Program of China(No.2022YFD1800400)National Natural Science Foundation of China(No.81991531)+1 种基金Natural Science Foundation of Shandong Province(No.ZR2020MC188)The sequence data of 25 K.pneumoniae isolates have been submitted to NCBI under BioProject accession number PRJNA1011004.
文摘The rapid spread of mobile tigecycline resistance presents a significant public health threat,particularly with the increasing prevalence of tet(X4)-positive Enterobacterales across various species.This study aimed to inves-tigate the epidemic features and transmission dynamics of tet(X4)-positive Klebsiella pneumoniae(K.pneumo-niae)through the analysis of 206 raw meats,including pork(n=182),beef(n=16),duck(n=5),and chicken(n=3).These samples were collected from schools,markets,and restaurants in Chengdu City,China.A total of 25 isolates were obtained from 13 administrative regions.All isolates exhibited resistance to tetracycline,tigecycline,ampicillin,chloramphenicol,and florfenicol.Over half of the isolates also demon-strated resistance to streptomycin(80%),sulfamethoxazole/trimethoprim(72%),ciprofloxacin(64%),and ampicillin/sulbactam(56%).Among these strains,14 distinct sequence types(STs)were identified,revealing evidence of inter-regional clonal spread,notably among 9 K.pneumoniae ST3393.Phylogenetic analysis revealed the presence of two K.pneumoniae ST5 closely resembling hypervirulent K.pneumoniae from Jiangsu.Importantly,12 isolates were capable of transferring tigecycline resistance to Escherichia coli J53.Further plasmid analysis showed that the tet(X4)-harboring plasmids in K.pneumoniae could be classified into four types,primarily belonging to the IncFIA(HI1)/HI1A/HI1B hybrid plasmid(n=16)and IncFII plasmid(n=7),which significantly contributed to the cross-species dissemination of tet(X4).In summary,this study highlights the prevalence of MDR tet(X4)-positive K.pneumoniae in Chengdu,driven predominantly by clonal expansion and plasmid-mediated horizontal gene transfer.These findings emphasize the importance of contin-uous surveillance of tet(X4)-positive K.pneumoniae in raw meat and the implementation of effective measures to control their spread.
基金funded by Laboratory of Lingnan Modern Agriculture Project(Award number NT2021006)supported by the National Natural Science Foundation of China(Award number 32102724)Pinduoduo-China Agricultural University Research Fund(No.PC2023A01002).
文摘This study aimed to investigate the protective effect of the nature product osthole(OST)against Clostridium perfrin-gens type A infection-caused myonecrosis in a mouse model.Male mice were divided into(1)control,(2)infected,(3)OST50 and(4)OST100 treatment groups.In the infected groups,mice were intramuscularly injected with 1×10^(8) CFU of C.perfringens per day for 6 days.Mice in the OST50 and OST100 groups were administrated intraperitoneally with OST at the doses of 50 or 100 mg/kg per day post C.perfringens infection.Our results showed that C.perfringens infection caused marked necrosis and inflammatory cell infiltration in the muscle tissues of mice.Mice in the OST50 and OST100 treatment groups displayed significantly attenuated C.perfringens infection-induced lipid peroxida-tion,oxidative stress,and apoptosis in their muscle tissue.Furthermore,OST treatment significantly downregulated the expressions of NF-κB,IL-1β,and TNF-αmRNA and protein levels,while concomitantly upregulating the expressions of Nrf2 and HO-1 mRNA and protein.OST treatments also inhibited the expression of phosphorylation(p)-p38,p-mTOR,and p-Erk1/2 proteins,and upregulated LC3II and Beclin1 proteins.In summary,our results reveal that OST therapy confers a protective effect against C.perfringens infection-induced oxidative stress and inflammation in muscle tissue,via activation of Nrf2/HO-1 and autophagy pathways and inhibition of p38,Erk1/2 and NF-κB pathways.
