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大豆花叶病毒半夏分离物侵染性克隆构建及鉴定 被引量:6
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作者 张丽 王德富 +2 位作者 裴燕妮 咸珅 牛颜冰 《生物工程学报》 CAS CSCD 北大核心 2020年第5期949-958,共10页
大豆花叶病毒(Soybean mosaic virus,SMV)作为半夏(Pinellia ternata(Thunb.)Breit.)主要病毒病害之一,已对其产量和品质造成严重影响。构建病毒侵染性克隆是反向遗传学研究病毒基因功能、病毒与宿主相互作用的有力工具,为明确SMV侵染... 大豆花叶病毒(Soybean mosaic virus,SMV)作为半夏(Pinellia ternata(Thunb.)Breit.)主要病毒病害之一,已对其产量和品质造成严重影响。构建病毒侵染性克隆是反向遗传学研究病毒基因功能、病毒与宿主相互作用的有力工具,为明确SMV侵染半夏的分子机制,开展SMV全长cDNA侵染性克隆的构建特别重要。因此文中利用Gibson体外重组系统对大豆花叶病毒山西半夏分离物(SMV-SXBX)侵染性克隆进行组装,通过农杆菌浸润法接种健康半夏;进一步通过机械传代、逆转录-聚合酶链式反应(RT-PCR)证实SMV-SXBX侵染性克隆3′末端含有poly(A)尾56 nt时具有稳定侵染性。该方法便捷、高效,且避免了SMV侵染性克隆在大肠杆菌中的不稳定问题。SMV全长侵染性cDNA克隆的构建,为进一步研究SMV复制和发病的分子机制奠定了基础。 展开更多
关键词 侵染性克隆 大豆花叶病毒 Gibson组装 多聚腺苷酸尾 半夏
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浓度梯度微流控芯片在药物筛选中的应用 被引量:1
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作者 张芳娟 刘海兵 +3 位作者 高梦琪 王德富 牛颜冰 申少斐 《化学进展》 SCIE CAS CSCD 北大核心 2021年第7期1138-1151,共14页
微流控芯片技术作为21世纪极具代表性的微型分析平台技术之一,以其试剂消耗低、分析微型化、可集成化、易于控制、自动化和良好的生物相容性等优点而成为研究热点,在生物、医学、食品和环境等多个领域都有杰出表现,尤其是药物筛选领域... 微流控芯片技术作为21世纪极具代表性的微型分析平台技术之一,以其试剂消耗低、分析微型化、可集成化、易于控制、自动化和良好的生物相容性等优点而成为研究热点,在生物、医学、食品和环境等多个领域都有杰出表现,尤其是药物筛选领域。其中备受关注的浓度梯度微流控芯片更是取得了显著成果。本文综述了近年来用于药物筛选的浓度梯度纸基芯片、浓度梯度水凝胶芯片、浓度梯度液滴芯片、浓度梯度聚二甲基硅氧烷(PDMS)芯片的研究进展;同时对浓度梯度微流控芯片在单细胞分析、组合药物筛选、三维(3D)细胞培养和细胞微环境模拟等方面的应用及优缺点进行了阐述,并在此基础上对其发展前景进行了展望。 展开更多
关键词 微流控芯片 浓度梯度 药物筛选
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Hyperfine structures and the field effects of IBr molecule in its rovibronic ground state
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作者 defu wang Xuping Shao +2 位作者 Yunxia Huang Chuanliang Li Xiaohua Yang 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第11期304-308,共5页
Hyperfine structures and the field effects of IBr molecule in its rovibronic ground state are theoretically studied by diagonalizing the effective Hamiltonian matrix.Perturbations of high-J levels up to 4 are taken in... Hyperfine structures and the field effects of IBr molecule in its rovibronic ground state are theoretically studied by diagonalizing the effective Hamiltonian matrix.Perturbations of high-J levels up to 4 are taken into account when studying the hyperfine sub-levels of the J=0 level,and thus,an 80×80 matrix is constructed and solved.Some of the experimentally absent molecular constants are computed using Dalton program.Our results will be helpful in the experimental investigation of manipulation and further cooling of cold IBr molecules. 展开更多
关键词 hyperfine structure Zeeman effect Stark effect IBr molecule
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Mixed-field effect at the hyperfine level of^(127)I^(79)Br in its rovibronic ground state:Toward field manipulation of cold molecules
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作者 包正斌 王得富 +2 位作者 邵旭萍 黄云霞 杨晓华 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第12期312-317,共6页
The mixed-field effect at the hyperfine level of the rovibronic ground state of the^(127)I^(79)Br(X^(1)Σ,v=0,J=0)molecule is computed on the J-I uncoupled basis of|JM_(J)I_(1)M_(1)I_(2)M_(2)>,where J is the molecu... The mixed-field effect at the hyperfine level of the rovibronic ground state of the^(127)I^(79)Br(X^(1)Σ,v=0,J=0)molecule is computed on the J-I uncoupled basis of|JM_(J)I_(1)M_(1)I_(2)M_(2)>,where J is the molecular total angular momentum excluding nuclear spin,M_J is the projection number of J,I_(1) and I_(2) are the nuclear spins of the iodine and bromine atoms,and M_(1) and M_(2) are the projection numbers of I_(1) and I_(2),respectively.When the two applied electric and magnetic fields are parallel,the perturbations are rare and only one perturbation is observed in a relatively large field regime in our computation range.However,when the two fields are off-parallel,the perturbations increase significantly and some sublevels show the Feshbach-like resonance phenomenon.Therefore,such sublevels transit between weak-field seeking and strong-field seeking repeatedly,which can be utilized to enhance or suppress cold molecular collision and chemical reaction rates.Such behavior of the molecular hyperfine structure in the mixed off-parallel fields may also be utilized to construct an electric-field-assisted anti-Helmholtz magnetic trap for cold molecules and to realize evaporative cooling of cold molecules(sub-mK)into the ultracold regime(μK). 展开更多
关键词 hyperfine structure mixed Zeeman and Stark effect evaporative cooling IBr molecule
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Effect of impeller rotational speed and paddle angle on binary mixing in a paddle mixer based on DEM
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作者 defu wang Jiaqi Yu +5 位作者 Zhijun Lv Yanhui Song Huinan Huang Hui Tian Haibo wang Baohao Su 《Particuology》 2025年第7期178-189,共12页
The discrete element method(DEM)and experimental measurements were employed to investigate the influence of impeller rotational speed and paddle angle on the mixing characteristics of two types of non-spherical partic... The discrete element method(DEM)and experimental measurements were employed to investigate the influence of impeller rotational speed and paddle angle on the mixing characteristics of two types of non-spherical particles in a paddle mixer.A close agreement between the simulation and experimental data was observed.By analyzing the motion of particles,it was found that a wide range of diffusion motion occurred when the particles pushed by the paddle blades rolled down the surface of the upper flowing layer of particles in the vessel,which caused the strong circumferential motion of particles,meanwhile promoted the axial movement of particles.The mixing characteristics were analyzed using relative standard deviation(RSD),velocity frequency distribution,diffusivity coefficient and Peclet numbers.The mixing process was accelerated at the rotational speeds of 15,20,and 25 rpm,meanwhile the superior mixing performance was achieved at the paddle angles of 30°and 45°in the study.The fluidity of particles in circumferential direction was higher than that in axial direction.Diffusion was the prevailing mixing mechanism in the current mixing system. 展开更多
关键词 Paddle mixer Discrete element method(DEM) Non-spherical particle Particle motion Mixing characteristics
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Study on round rice straw bale wrapping silage technology and facilities 被引量:5
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作者 Liqiao Li defu wang Xing Yang 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2018年第4期88-95,共8页
Specific to problems of the existing round bale wrapping machine in China,such as small application scope and failing to satisfy the wrapping demands of round bales of different specifications,a tumbler-type round bal... Specific to problems of the existing round bale wrapping machine in China,such as small application scope and failing to satisfy the wrapping demands of round bales of different specifications,a tumbler-type round bale wrapping machine based on wrapping silage with stretch film was designed.By theoretical analysis and preliminary experiments of the corresponding wrapping process,revolving speed of tumbler,speed ratio between tumbler and carrier rollers,and pre-load force of stretch film were determined as experimental factors,while wrapping time and consumption of stretch film per unit mass of round bale were determined as evaluation indexes.Furthermore,quadratic regression orthogonal rotational combination experimental design was adopted to carry out the wrapping experiment.The regression models were set up,and influencing rules of experimental factors on evaluation indexes were also analyzed.The optimal parameter combination scheme was revolving speed of tumbler of 31-32 r/min,speed ratio between tumbler and carrier rollers of 2.6,and pre-load force of stretch film of 20-22 N.Under such a circumstance,the corresponding wrapping time required was 37.7-38.9 s and consumption of stretch film per unit mass of round bale was 3.21-3.46 kg/t.The predicted values and the measured values of evaluation indexes basically coincided with each other and the relative error fell below 5%,which indicated that both the optimized regression models acquired through the wrapping experiment and the relevant parameter optimization results were reliable.To guarantee the quality of rice straw silage,the round bale should be wrapped by no less than five layers of stretch films through the utilization of the tumbler-type round bale wrapping machine.The study results could be used as references for research and development of the round bale wrapping machine. 展开更多
关键词 round bale SILAGE wrapping machine tumbler-type analysis experiment
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The decoupling between gas exchange and water potential of Cinnamomum camphora seedlings during drought recovery and its relation to ABA accumulation in leaves 被引量:2
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作者 Honglang Duan defu wang +7 位作者 Xiaohua Wei Guomin Huang Houbao Fan Shuangxi Zhou Jianping Wu Wenfei Liu David TTissue Songze Wan 《Journal of Plant Ecology》 SCIE CSCD 2020年第6期683-692,共10页
Aims Drought stress and the degree of drought severity are predicted to rise under highly variable patterns of precipitation due to climate change,while the capacity of trees to cope with drought recovery through phys... Aims Drought stress and the degree of drought severity are predicted to rise under highly variable patterns of precipitation due to climate change,while the capacity of trees to cope with drought recovery through physiological and biochemical adjustment remains unclear.We aimed to examine the coupling of physiology and biochemistry in trees during drought and the following recovery.Methods Potted seedlings of Cinnamomum camphora were grown under well watered conditions prior to the experimental drought stress,which was initiated by withholding water.Seedlings were rewatered following attainment of two drought severities:mild drought(stomatal closure)and moderate drought(ψxylem=−1.5 MPa).We measured leaf-level water potential,gas exchange(photosynthesis and stomatal conductance),abscisic acid(ABA),proline and non-structural carbohydrates(NSCs)concentrations in seedlings of C.camphora during drought and a 4-day recovery.Important Findings We found that drought severity largely determined physiological and biochemical responses and affected the rate of recovery.Stomatal closure occurred at the mild drought stress,accompanied with ABA accumulation in leaves and decline in water potential,while leaf proline accumulation and variable NSC were evident at the moderate drought stress.More severe drought stress led to delayed recovery of gas exchange,but it did not have significant effect on water potential recovery.The relationships of water potential and gas exchange differed during drought stress and post-drought recovery.There was tight coupling between water potential and gas exchange during drought,but not during rewatering due to high ABA accumulation in leaves,thereby delaying recovery of stomatal conductance.Our results demonstrate that ABA could be an important factor in delaying the recovery of stomatal conductance following rewatering and after water potential recovery of C.camphora.Furthermore,greater drought severity had significant impacts on the rate of recovery of tree physiology and biochemistry. 展开更多
关键词 DROUGHT RECOVERY gas exchange ABA NSC PROLINE
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Fault diagnosis of silage harvester based on a modified random forest 被引量:2
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作者 Xiuli Zhou Xiaochuan Xu +3 位作者 Junfeng Zhang Ling wang defu wang Pingping Zhang 《Information Processing in Agriculture》 EI CSCD 2023年第3期301-311,共11页
The objective of this study is to investigate the effectiveness of a multi-parameter intelligent fault diagnosis method based on a modified random forest algorithm(RFNB algorithm),so as to reduce the impact of blockag... The objective of this study is to investigate the effectiveness of a multi-parameter intelligent fault diagnosis method based on a modified random forest algorithm(RFNB algorithm),so as to reduce the impact of blockage fault on the operation of a silage harvester,thus providing a reference for the intelligent control.In brief,the forward speed,cutting speed,engine speed and engine load were selected as the input variables.Then,a random forest(RF)was used to construct a naive Bayes classifier for each node of the decision tree,and finally the RFNB algorithm constituted based on the naive Bayes tree(NBTree).The results revealed that by improving the classification accuracy of a single decision tree,the fault diagnosis accuracy of the entire RF was improved.When the sample data were consistent,the accuracy of the RFNB algorithm was 97.9%,while that of the RF algorithm was only 93.27%.Besides,the performance of RFNB classifiers was significantly better than that of RF classifiers.In conclusion,the RFNB model can accurately identify the fault status of the silage harvester with its good robustness,which provides a new idea for the fault monitoring and early warning of large agricultural rotating machinery in the future. 展开更多
关键词 Random forest Silage harvester Fault diagnosis NBTree
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