Stipes are the major waste from the processing of Lentinus edodes.To make full use of L.edodes stipes(LES),differ-ent fractions of LES polysaccharides(LESPs)were first obtained by water extraction and gradient ethanol...Stipes are the major waste from the processing of Lentinus edodes.To make full use of L.edodes stipes(LES),differ-ent fractions of LES polysaccharides(LESPs)were first obtained by water extraction and gradient ethanol precipitation.Afterwards,the LES residues were treated with an optimal combination of Aspergillus niger and Saccharomyces cerevisiae for the preparation of soluble/insoluble dietary fibers(LESS/LESI)using the response surface methodology and the Box-Behnken design.Subsequently,the in vitro immunomodulatory activity of LESPs and LESS,as well as the adsorption capacities of LESI were evaluated.The results showed that LESPs were neutral polysaccharides,mainly containing glucose.The optimal parameters for modifying the residues of LES were the followings:4%(w/w)A.niger,8%(w/w)S.cerevisiae,31℃,3 d,and a solid-liquid ratio of 1:12.5 in a yield of 14.73%/82.45%of LESS/LESI,respectively.The in vitro immunomodulatory activity assays revealed that LESPs and LESS had potent immunostimulatory activity to increase phagocytosis,acid phosphatase activity and nitric oxide production of RAW264.7 murine cell macrophages.The evaluation of adsorption capacities revealed that LESI owned stronger water holding capacity,oil holding capacity and water swelling capacity.This research could provide an effective way to fully utilize discarded L.edodes stipes with high added-value.展开更多
基金supported by the National Key Research and Development Program of China(The integration and demonstration of key technologies of characteristic cash crops,fruit and vegetable industry in Qinba Mountain area of Shaanxi Province,No.2022YFD1602000)the National Natural Science Foundation of China(No.32272428,31801491)+4 种基金the Scientific Program Funded by Xi’an Science and Technology Bureau(No.22NYYF032)Young Talent Lifting Project Funded by Shaanxi Association for Science and Technology(No.20220216)National Foreign Expert Project Funded by the Ministry of Science and Technology of China(G2022041012L)Xi’an Weiyang District of Technology and Industrial Information Bureau(No.202134)Shaanxi Administration of Traditional Chinese Medicine(SZY-KJCYC-2023-014).
文摘Stipes are the major waste from the processing of Lentinus edodes.To make full use of L.edodes stipes(LES),differ-ent fractions of LES polysaccharides(LESPs)were first obtained by water extraction and gradient ethanol precipitation.Afterwards,the LES residues were treated with an optimal combination of Aspergillus niger and Saccharomyces cerevisiae for the preparation of soluble/insoluble dietary fibers(LESS/LESI)using the response surface methodology and the Box-Behnken design.Subsequently,the in vitro immunomodulatory activity of LESPs and LESS,as well as the adsorption capacities of LESI were evaluated.The results showed that LESPs were neutral polysaccharides,mainly containing glucose.The optimal parameters for modifying the residues of LES were the followings:4%(w/w)A.niger,8%(w/w)S.cerevisiae,31℃,3 d,and a solid-liquid ratio of 1:12.5 in a yield of 14.73%/82.45%of LESS/LESI,respectively.The in vitro immunomodulatory activity assays revealed that LESPs and LESS had potent immunostimulatory activity to increase phagocytosis,acid phosphatase activity and nitric oxide production of RAW264.7 murine cell macrophages.The evaluation of adsorption capacities revealed that LESI owned stronger water holding capacity,oil holding capacity and water swelling capacity.This research could provide an effective way to fully utilize discarded L.edodes stipes with high added-value.
