Rainwater harvesting RWH has yet to see significant uptake in UK households primarily due to a lack of innovation in residential RWH system types.This ppa er presents the results of per liminary investigations into a ...Rainwater harvesting RWH has yet to see significant uptake in UK households primarily due to a lack of innovation in residential RWH system types.This ppa er presents the results of per liminary investigations into a range of traditional and innovative residential-scale RWH systems. These systems are examined using a patent application search informal interviews with industry professionals cost-benefit analysis and a simple multi criteria analysis MCA .The latter examines the sustainability of the systems based on a priori social economic and ne vironmenat l criteria.Two of the innovative systems are subject to a more detailed analysis and benchmarked agains ta rt aditional system.Results of the MCA indicate that the innovative RWH systems achieve better sustainability scores than the traditional RWH with a lower capital cost.Further research is focused on monitoring the identified systems to generate empir cal datasets in order to undertake the WLC/LCAs and to identify challenges associated with installation.展开更多
Zika virus(ZIKV)causes significant human diseases without specific therapy.Previously we found erythrosin B,an FDA-approved food additive,inhibited viral NS2B−NS3 interactions,leading to inhibition of ZIKV infection i...Zika virus(ZIKV)causes significant human diseases without specific therapy.Previously we found erythrosin B,an FDA-approved food additive,inhibited viral NS2B−NS3 interactions,leading to inhibition of ZIKV infection in cell culture.In this study,we performed pharmacokinetic and in vivo studies to demonstrate the efficacy of erythrosin B against ZIKV in 3D mini-brain organoid and mouse models.Our results showed that erythrosin B is very effective in abolishing ZIKV replication in the 3D organoid model.Although pharmacokinetics studies indicated that erythrosin B had a low absorption profile,mice challenged by a lethal dose of ZIKV showed a significantly improved survival rate upon oral administration of erythrosin B,compared to vehicle control.Limited structure−activity relationship studies indicated that most analogs of erythrosin B with modifications on the xanthene ring led to loss or reduction of inhibitory activities towards viral NS2B−NS3 interactions,protease activity and antiviral efficacy.In contrast,introducing chlorine substitutions on the isobenzofuran ring led to slightly increased activities,suggesting that the isobenzofuran ring is well tolerated for modifications.Cytotoxicity studies indicated that all derivatives are nontoxic to human cells.Overall,our studies demonstrated erythrosin B is an effective antiviral against ZIKV both in vitro and in vivo.展开更多
Waterborne viral epidemics are a major threat to public health.Increasing interest in wastewater reclamation highlights the importance of understanding the health risks associated with potential microbial hazards,part...Waterborne viral epidemics are a major threat to public health.Increasing interest in wastewater reclamation highlights the importance of understanding the health risks associated with potential microbial hazards,particularly for reused water in direct contact with humans.This study focused on identifying viral epidemic patterns in municipal wastewater reused for recreational applications based on long-term,spatially explicit global literature data during 2000e2021,and modelled human health risks from multiple exposure pathways using a well-established quantitative microbial risk assessment methodology.Global median viral loads in municipal wastewater ranged from 7.92×10^(4)to 1.4×10^(6)GC L^(-1)in the following ascending order:human adenovirus(HAdV),norovirus(NoV)GII,enterovirus(EV),NoV GI,rotavirus(RV),and severe acute respiratory syndrome coronavirus 2(SARSCoV-2).Following secondary or tertiary wastewater treatment,NoV GI,NoV GII,EV,and RV showed a relatively higher and more stable log reduction value with medians all above 0.8(84%),whereas SARSCoV-2 and HAdV showed a relatively lower reduction,with medians ranging from 0.33(53%)to 0.55(72%).A subsequent disinfection process effectively enhanced viral removal to over 0.89-log(87%).The predicted event probability of virus-related gastrointestinal illness and acute febrile respiratory illnesses in reclaimed recreational water exceeded the World Health Organization recommended recreational risk benchmark(5%and 1.9%,respectively).Overall,our results provided insights on health risks associated with reusing wastewater for recreational purposes and highlighted the need for establishing a regulatory framework ensuring the safety management of reclaimed waters.展开更多
基金Severn Trent Water and the EPSRC as part of the STREAM Engineering Doctorate Program
文摘Rainwater harvesting RWH has yet to see significant uptake in UK households primarily due to a lack of innovation in residential RWH system types.This ppa er presents the results of per liminary investigations into a range of traditional and innovative residential-scale RWH systems. These systems are examined using a patent application search informal interviews with industry professionals cost-benefit analysis and a simple multi criteria analysis MCA .The latter examines the sustainability of the systems based on a priori social economic and ne vironmenat l criteria.Two of the innovative systems are subject to a more detailed analysis and benchmarked agains ta rt aditional system.Results of the MCA indicate that the innovative RWH systems achieve better sustainability scores than the traditional RWH with a lower capital cost.Further research is focused on monitoring the identified systems to generate empir cal datasets in order to undertake the WLC/LCAs and to identify challenges associated with installation.
基金This study was partially supported by grants AI131669,AI140726,and AI141178 from the National Institute of Allergy and Infectious Diseases(NIAID,USA)the National Institutes of Health(Hongmin Li and Jia Zhou)+2 种基金Additionally,Jia Zhou is partly supported by the John D.Stobo,M.D.Distinguished Chair Endowment Fund at UTMBHongmin Li is additionally supported by NIH grants AI133219,AI134568,AI140406,and AI140491,USAthe R.Ken and Donna Coit Endowed Chair fund in Drug Discovery.
文摘Zika virus(ZIKV)causes significant human diseases without specific therapy.Previously we found erythrosin B,an FDA-approved food additive,inhibited viral NS2B−NS3 interactions,leading to inhibition of ZIKV infection in cell culture.In this study,we performed pharmacokinetic and in vivo studies to demonstrate the efficacy of erythrosin B against ZIKV in 3D mini-brain organoid and mouse models.Our results showed that erythrosin B is very effective in abolishing ZIKV replication in the 3D organoid model.Although pharmacokinetics studies indicated that erythrosin B had a low absorption profile,mice challenged by a lethal dose of ZIKV showed a significantly improved survival rate upon oral administration of erythrosin B,compared to vehicle control.Limited structure−activity relationship studies indicated that most analogs of erythrosin B with modifications on the xanthene ring led to loss or reduction of inhibitory activities towards viral NS2B−NS3 interactions,protease activity and antiviral efficacy.In contrast,introducing chlorine substitutions on the isobenzofuran ring led to slightly increased activities,suggesting that the isobenzofuran ring is well tolerated for modifications.Cytotoxicity studies indicated that all derivatives are nontoxic to human cells.Overall,our studies demonstrated erythrosin B is an effective antiviral against ZIKV both in vitro and in vivo.
基金supported by the National Natural Science Foundation of China(No.51922013)the Shenzhen Science and Technology Innovation Commission,China(No.KQTD201909209172630447,No.GXWD20220818172959001,No.KCXST20221021111404011).
文摘Waterborne viral epidemics are a major threat to public health.Increasing interest in wastewater reclamation highlights the importance of understanding the health risks associated with potential microbial hazards,particularly for reused water in direct contact with humans.This study focused on identifying viral epidemic patterns in municipal wastewater reused for recreational applications based on long-term,spatially explicit global literature data during 2000e2021,and modelled human health risks from multiple exposure pathways using a well-established quantitative microbial risk assessment methodology.Global median viral loads in municipal wastewater ranged from 7.92×10^(4)to 1.4×10^(6)GC L^(-1)in the following ascending order:human adenovirus(HAdV),norovirus(NoV)GII,enterovirus(EV),NoV GI,rotavirus(RV),and severe acute respiratory syndrome coronavirus 2(SARSCoV-2).Following secondary or tertiary wastewater treatment,NoV GI,NoV GII,EV,and RV showed a relatively higher and more stable log reduction value with medians all above 0.8(84%),whereas SARSCoV-2 and HAdV showed a relatively lower reduction,with medians ranging from 0.33(53%)to 0.55(72%).A subsequent disinfection process effectively enhanced viral removal to over 0.89-log(87%).The predicted event probability of virus-related gastrointestinal illness and acute febrile respiratory illnesses in reclaimed recreational water exceeded the World Health Organization recommended recreational risk benchmark(5%and 1.9%,respectively).Overall,our results provided insights on health risks associated with reusing wastewater for recreational purposes and highlighted the need for establishing a regulatory framework ensuring the safety management of reclaimed waters.
