Background The sterile insect technique(SIT)requires distinguishing sterile from wild male mosquitoes to evaluate male qualities and maintain an appropriate release ratio for efficient population suppression.Current d...Background The sterile insect technique(SIT)requires distinguishing sterile from wild male mosquitoes to evaluate male qualities and maintain an appropriate release ratio for efficient population suppression.Current dye/powder marking methods have limitations and may affect SIT effectiveness,necessitating alternative discrimination strategies.Aedes albopictus naturally harbors two Wolbachia infections(wAlbA/wAlbB),which can be eliminated via tetracycline.Although Wolbachia removal minimally affect host fitness,its impact on microbiota remains unclear.Characterizing post-elimination microbial communities is the first step to identify novel endogenous biomarkers for SIT monitoring.Methods We analyzed the bacterial diversity and composition of two strains of wild-type GUA(Wolbachia-infected)and GT(Wolbachia-free)mosquitoes using the 16S rRNA V3-V4 region sequencing.qPCR was employed to confirm the relative abundance of four major bacterial genera,while PCR was used to validate selected biomarkers for distinguishing factory-reared sterile males from wild males.Kruskal-Wallis or Mann-Whitney test was used to analyze the comparable parameters between GUA and GT strains.Results Five-day-old GUA and GT females showed similar microbial diversity/composition,while young males shared diversity but differed in composition.The core microbiota in both strains consisted of Proteobacteria(64.27%),Firmicutes(16.09%),Actinobacteriota(11.22%),and Bacteroidota(4.96%).Asaia was dominant in both strains(GUA:47.33%;GT:32.69%),whereas Enterococcus increased in GT males with aging.Wolbachia was absent in GT mosquitoes,and Elizabethkingia was undetected in GUA males.qPCR further confirmed these trends.PCR analysis revealed that wAlbB exhibited higher stability in differentiating factory-reared GT males from their wild counterparts(96.7%infection in field males,n=60)compared to wAlbA(61.7%,n=60)or Enterococcus(65.8%,n=120).The mark-releaserecapture experiment further confirmed the detectability using wAlbB biomarker.Conclusions Without obvious fitness costs observed previously in the Ae.albopictus GT strain compared to GUA strain,the removal of Wolbachia significantly changes the microbial composition in male mosquitoes in this study.Wolbachia wAlbB is recommended as a reliable biomarker for distinguishing sterile males from wild males when using GT strain in SIT programs targeting Ae.albopictus.展开更多
Background Gastropoda,the largest class within the phylum Mollusca,houses diverse gut microbiota,and some gastropods serve as intermediate hosts for parasites.Studies have revealed that gut bacteria in gastropods are ...Background Gastropoda,the largest class within the phylum Mollusca,houses diverse gut microbiota,and some gastropods serve as intermediate hosts for parasites.Studies have revealed that gut bacteria in gastropods are associated with various biological aspects,such as growth,immunity and host-parasite interactions.Here,we summarize our current knowledge of gastropod gut microbiomes and highlight future research priorities and perspectives.Methods A literature search was undertaken using PubMed,Web of Science and CNKI for the articles on the gut microbiota of gastropods until December 31,2022.We retrieved a total of 166 articles and identified 73 eligible articles for inclusion in this review based on the inclusion and exclusion criteria.Results Our analysis encompassed freshwater,seawater and land snails,with a specific focus on parasite-transmitting gastropods.We found that most studies on gastropod gut microbiota have primarily utilized 16S rRNA gene sequencing to analyze microbial composition,rather than employing metagenomic,metatranscriptomic,or metabolomic approaches.This comprehensive review provided an overview of the parasites carried by snail species in the context of gut microbiota studies.We presented the gut microbial trends,a comprehensive summary of the diversity and composition,influencing factors,and potential functions of gastropod gut microbiota.Additionally,we discussed the potential applications,research gaps and future perspectives of gut microbiomes in parasite-transmitting gastropods.Furthermore,several strategies for enhancing our comprehension of gut microbiomes in snails were also discussed.Conclusions This review comprehensively summarizes the current knowledge on the composition,potential function,influencing factors,potential applications,limitations,and challenges of gut microbiomes in gastropods,with a specific emphasis on parasite-transmitting gastropods.These findings provide important insights for future studies aiming to understand the potential role of gastropod gut microbiota in controlling snail populations and snail-borne diseases.展开更多
基金funded by the NSFC-BMGF(82261128006 and 2022YFML1005)the Guangzhou Basic and Applied Basic Research Foundation(SL2024A04J01715)+1 种基金the 6th Nuclear Energy R&D Project(20201192)the IAEA Department of Technical Cooperation(RAS5095)and the IAEA Coordinated Research Project(D44005).
文摘Background The sterile insect technique(SIT)requires distinguishing sterile from wild male mosquitoes to evaluate male qualities and maintain an appropriate release ratio for efficient population suppression.Current dye/powder marking methods have limitations and may affect SIT effectiveness,necessitating alternative discrimination strategies.Aedes albopictus naturally harbors two Wolbachia infections(wAlbA/wAlbB),which can be eliminated via tetracycline.Although Wolbachia removal minimally affect host fitness,its impact on microbiota remains unclear.Characterizing post-elimination microbial communities is the first step to identify novel endogenous biomarkers for SIT monitoring.Methods We analyzed the bacterial diversity and composition of two strains of wild-type GUA(Wolbachia-infected)and GT(Wolbachia-free)mosquitoes using the 16S rRNA V3-V4 region sequencing.qPCR was employed to confirm the relative abundance of four major bacterial genera,while PCR was used to validate selected biomarkers for distinguishing factory-reared sterile males from wild males.Kruskal-Wallis or Mann-Whitney test was used to analyze the comparable parameters between GUA and GT strains.Results Five-day-old GUA and GT females showed similar microbial diversity/composition,while young males shared diversity but differed in composition.The core microbiota in both strains consisted of Proteobacteria(64.27%),Firmicutes(16.09%),Actinobacteriota(11.22%),and Bacteroidota(4.96%).Asaia was dominant in both strains(GUA:47.33%;GT:32.69%),whereas Enterococcus increased in GT males with aging.Wolbachia was absent in GT mosquitoes,and Elizabethkingia was undetected in GUA males.qPCR further confirmed these trends.PCR analysis revealed that wAlbB exhibited higher stability in differentiating factory-reared GT males from their wild counterparts(96.7%infection in field males,n=60)compared to wAlbA(61.7%,n=60)or Enterococcus(65.8%,n=120).The mark-releaserecapture experiment further confirmed the detectability using wAlbB biomarker.Conclusions Without obvious fitness costs observed previously in the Ae.albopictus GT strain compared to GUA strain,the removal of Wolbachia significantly changes the microbial composition in male mosquitoes in this study.Wolbachia wAlbB is recommended as a reliable biomarker for distinguishing sterile males from wild males when using GT strain in SIT programs targeting Ae.albopictus.
基金supported by the National Key R&D Program of China(Nos.2020YFC1200100,2020YFC1200103,2021YFC2300800 and 2016YFC1200500)the National Natural Science Foundation of China(Nos.82202560,82161160343 and 82272361)+5 种基金the R&D Program in Key Areas of Guangdong Province(No.2022B1111030002)the Fundamental Research Funds for the Central University(No.22qntd4813)the Science and Technology Planning Project of Guangdong Province(No.2021B1212040017)the 111 Project(No.B12003)the 6th Nuclear Energy R&D Project(No.20201192)the National Parasitic Resource Center and Ministry of Science and Technology(No.NPRC-2019-194-30).
文摘Background Gastropoda,the largest class within the phylum Mollusca,houses diverse gut microbiota,and some gastropods serve as intermediate hosts for parasites.Studies have revealed that gut bacteria in gastropods are associated with various biological aspects,such as growth,immunity and host-parasite interactions.Here,we summarize our current knowledge of gastropod gut microbiomes and highlight future research priorities and perspectives.Methods A literature search was undertaken using PubMed,Web of Science and CNKI for the articles on the gut microbiota of gastropods until December 31,2022.We retrieved a total of 166 articles and identified 73 eligible articles for inclusion in this review based on the inclusion and exclusion criteria.Results Our analysis encompassed freshwater,seawater and land snails,with a specific focus on parasite-transmitting gastropods.We found that most studies on gastropod gut microbiota have primarily utilized 16S rRNA gene sequencing to analyze microbial composition,rather than employing metagenomic,metatranscriptomic,or metabolomic approaches.This comprehensive review provided an overview of the parasites carried by snail species in the context of gut microbiota studies.We presented the gut microbial trends,a comprehensive summary of the diversity and composition,influencing factors,and potential functions of gastropod gut microbiota.Additionally,we discussed the potential applications,research gaps and future perspectives of gut microbiomes in parasite-transmitting gastropods.Furthermore,several strategies for enhancing our comprehension of gut microbiomes in snails were also discussed.Conclusions This review comprehensively summarizes the current knowledge on the composition,potential function,influencing factors,potential applications,limitations,and challenges of gut microbiomes in gastropods,with a specific emphasis on parasite-transmitting gastropods.These findings provide important insights for future studies aiming to understand the potential role of gastropod gut microbiota in controlling snail populations and snail-borne diseases.
