Winged bean(Psophocarpus tetragonolobus(L.)DC.)is a tropical legume crop found in Southeast Asian countries including Thailand.The mature seeds have high protein content and have been targeted as a protein source,and ...Winged bean(Psophocarpus tetragonolobus(L.)DC.)is a tropical legume crop found in Southeast Asian countries including Thailand.The mature seeds have high protein content and have been targeted as a protein source,and germination has been used to increase the value of nutrition.We used nanoLC/timsTOF Pro 2 combined with PEAKS®studio Xpro software to explore the proteomes of mature seeds and sprouts of purple winged bean.This study is the first report on the allergenic proteins in mature seeds and sprouts of purple winged bean.In silico analysis using Allergen Nomenclature search and the Allermatch^(tm) program,37 allergens were found.Conglutin beta 5 and late embryogenesis abundant protein(3 and D-29)were identified only in mature seeds,while 4 types of major pollen allergen Bet V,profilin and isoflavone reductase,were identified only in sprouts.Examples of allergens in both samples include basic 7S globulin,beta-conglycinin alpha/beta subunit(1,2),convicilin,defensin-like protein,7 types of non-specific lipid-transfer protein,P24 oleosin isoform B.Since food allergy and airway allergy are found in sprouts,we used shotgun proteomics and in silico analysis for detecting the profiles of allergens.With regards to food safety,purple winged beans may also induce cross-reactivity in patients who are allergic to peanuts or other legumes.Therefore,identifying the proteomes and potential allergens in mature seeds and sprouts of purple winged beans could assist researchers in studying plant-based food safety.展开更多
A shotgun proteomic and in-silico analysis was performed to identify and characterize the proteomes and potential allergens in both processed and non-processed forms of krill(Acetes spp.)and whiteleg shrimp(Litopenaeu...A shotgun proteomic and in-silico analysis was performed to identify and characterize the proteomes and potential allergens in both processed and non-processed forms of krill(Acetes spp.)and whiteleg shrimp(Litopenaeus vannamei).The samples were analyzed using LC-MS/MS,and the identified proteins were analyzed for protein-protein interactions and Gene Ontology.Potential allergens were analyzed by Allermatch and predicted IgE epitope by AlgPred.The results revealed that processed krill and whiteleg shrimp had a higher number of identified proteins and potential allergens compared to their non-processed forms.The major functions of identified proteins were related to organelle organization,intracellular and cytoskeletal protein binding.Eleven potential allergens were identified in all samples,indicating common allergens in both species.In-silico analysis of potential allergens of krill and whiteleg shrimp showed IgE epitopes on alpha-actinin sarcomeric-like isoform X1,tropomyosin fast isoform and tropomyosin isoform X19.The peptide IQLLEEDLER in tropomyosin fast isoform was identified as a potential allergen marker in fresh,dried and powdered forms of krill and whiteleg shrimp.These findings provide insight into the food safety,clinical and diagnostic aspects of krill and shrimp,particularly regarding its potential allergenicity and its allergen biomarkers.展开更多
基金supported by Thailand Science Research and Innovation(TSRI)and Chulabhorn Research Institute(Grant No.48291/4691915).
文摘Winged bean(Psophocarpus tetragonolobus(L.)DC.)is a tropical legume crop found in Southeast Asian countries including Thailand.The mature seeds have high protein content and have been targeted as a protein source,and germination has been used to increase the value of nutrition.We used nanoLC/timsTOF Pro 2 combined with PEAKS®studio Xpro software to explore the proteomes of mature seeds and sprouts of purple winged bean.This study is the first report on the allergenic proteins in mature seeds and sprouts of purple winged bean.In silico analysis using Allergen Nomenclature search and the Allermatch^(tm) program,37 allergens were found.Conglutin beta 5 and late embryogenesis abundant protein(3 and D-29)were identified only in mature seeds,while 4 types of major pollen allergen Bet V,profilin and isoflavone reductase,were identified only in sprouts.Examples of allergens in both samples include basic 7S globulin,beta-conglycinin alpha/beta subunit(1,2),convicilin,defensin-like protein,7 types of non-specific lipid-transfer protein,P24 oleosin isoform B.Since food allergy and airway allergy are found in sprouts,we used shotgun proteomics and in silico analysis for detecting the profiles of allergens.With regards to food safety,purple winged beans may also induce cross-reactivity in patients who are allergic to peanuts or other legumes.Therefore,identifying the proteomes and potential allergens in mature seeds and sprouts of purple winged beans could assist researchers in studying plant-based food safety.
基金supported by Thailand Science Research and Innovation(TSRI),Chulabhorn Research Institute(Grant No.36821/4274350)the Research Promotion and Technology Transfer Center(RPTTC),Faculty of Liberal Arts and Science,Kasetsart University,Kamphaeng Saen Campusand the Kasetsart University Research and Development Institute(KURDI),Kasetsart University(V-T(D)179.58)。
文摘A shotgun proteomic and in-silico analysis was performed to identify and characterize the proteomes and potential allergens in both processed and non-processed forms of krill(Acetes spp.)and whiteleg shrimp(Litopenaeus vannamei).The samples were analyzed using LC-MS/MS,and the identified proteins were analyzed for protein-protein interactions and Gene Ontology.Potential allergens were analyzed by Allermatch and predicted IgE epitope by AlgPred.The results revealed that processed krill and whiteleg shrimp had a higher number of identified proteins and potential allergens compared to their non-processed forms.The major functions of identified proteins were related to organelle organization,intracellular and cytoskeletal protein binding.Eleven potential allergens were identified in all samples,indicating common allergens in both species.In-silico analysis of potential allergens of krill and whiteleg shrimp showed IgE epitopes on alpha-actinin sarcomeric-like isoform X1,tropomyosin fast isoform and tropomyosin isoform X19.The peptide IQLLEEDLER in tropomyosin fast isoform was identified as a potential allergen marker in fresh,dried and powdered forms of krill and whiteleg shrimp.These findings provide insight into the food safety,clinical and diagnostic aspects of krill and shrimp,particularly regarding its potential allergenicity and its allergen biomarkers.
