A nickel hexacyanoferrate(NiHCF)film electrode was prepared with NiHCF,conductive carbon black,and polyvinylidene difluoride,which was coated on graphite plate substrate for selective extraction of Cs^(+)ions by using...A nickel hexacyanoferrate(NiHCF)film electrode was prepared with NiHCF,conductive carbon black,and polyvinylidene difluoride,which was coated on graphite plate substrate for selective extraction of Cs^(+)ions by using electrochemically switched ion exchange(ESIX)technology.A potential-responsive ionpump system for efficient extraction of Cs+ions was designed,and the effect of wet film thicknesses,charging modes,flow rates,and chamber widths on Cs+ions extraction performance was investigated.In the system,the adsorption capacity and removal percentage of Cs^(+)ions on the NiHCF film electrode reached as high as 147.69 mg·g^(-1)and 92.47%,respectively.Furthermore,the NiHCF film electrode showed high selectivity for Cs^(+)ions and stability.After seven cycles of adsorption/desorption,the desorption percentage could reach about 100%.The excellent Cs^(+)extraction performance should be attributed to the strong driving force produced by the potential-responsive ion-pumping effect in the ESIX process,as well as the low ion transfer resistance of the film electrode which is caused by the special crystal structure of NiHCF.In addition,the NiHCF film electrode was implemented to work together with the bismuth oxybromide(BiOBr)film electrode to accomplish the simultaneous extraction of Cs^(+)and Br^(-).And the adsorption capacity and removal percentage of Br^(-)ions on the BiOBr film electrode reached 69.53 mg·g^(-1)and 77.32%,correspondingly.It is expected that such a potential-responsive ion-pump system based on NiHCF and BiOBr film electrodes could be used for the selective extraction and concentration of Cs^(+)and Br^(-)ions from salt lake brine.展开更多
Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor...Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor.It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip.For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment,dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001μg·ml-1 and to 0.01μg·ml-1,respectively.Furthermore,when aflatoxin B1 concentrations are 0.1μg·ml-1,1μg·ml-1,10μg·ml-1 and 100μg·ml-1,dip amplitudes of 0.2179,0.226,0.2384 and 0.2527 and dip redshifts of 10.1 GHz,20.1 GHz,27.7 GHz and 37.6 GHz are respectively observed.These results illustrate high-sensitivity,label-free detection of aflatoxin B1,enriching the applications of sensors in the terahertz domain.展开更多
Food allergy is an emerging global health concern with limited treatment options.This study evaluated the efficacy of Limosilactobacillus reuteri(L.reuteri)FN041 in a cow’s milkβ-lactoglobulin-induced murine model o...Food allergy is an emerging global health concern with limited treatment options.This study evaluated the efficacy of Limosilactobacillus reuteri(L.reuteri)FN041 in a cow’s milkβ-lactoglobulin-induced murine model of food allergy.Daily oral administration of L.reuteri FN041 alleviated allergic symptoms,normalized body temperature,and reduced lung inflammation.L.reuteri FN041 lowered serum IgE levels,rebalanced Th1/Th2 cytokines,and expanded splenic regulatory T cells.Intestinal barrier integrity was improved,as evidenced by restored tight junction proteins and enhanced antioxidant capacity.Although the overall gut microbialα-andβ-diversity remained unchanged,L.reuteri FN041 selectively enriched Duncaniella muris and Muribaculum gor-doncarteri,while suppressing Waltera intestinalis and Lacrimispora xylanisolvens.Metabolomic profiling revealed an upregulation of anti-inflammatory flavonoids,particularly naringenin-7-O-glucoside.Mechanistically,L.reuteri FN041 inhibited AKT/mTOR and NF-κB signaling.These findings suggest that L.reuteri FN041 is a promising biotherapeutic for food allergy,acting through immune modulation,gut barrier protection,microbiota remodeling,and oxidative stress mitigation.展开更多
Limosilactobacillus reuteri(L.reuteri)FN041,a probiotic isolated from breast milk,exhibits immunomodulatory effects against allergic and immune-related diseases.However,the preventive and therapeutic potential of L.re...Limosilactobacillus reuteri(L.reuteri)FN041,a probiotic isolated from breast milk,exhibits immunomodulatory effects against allergic and immune-related diseases.However,the preventive and therapeutic potential of L.reuteri FN041 in ulcerative colitis(UC)remains unreported.This study investigates the protective effects of L.reuteri FN041 in a mouse model of colitis induced by dextran sodium sulfate(DSS).Significant alleviation of colitis symptoms was observed with L.reuteri FN041 treatment,demonstrated by decreased weight loss,reduced colon shortening,and lower Disease Activity Index and Histological Index scores.Inflammation and oxidative stress levels were reduced,as shown by lower local and systemic IL-6,elevated IL-10 and increased colonic malondialdehyde levels.The expression of tight junction proteins,indicators of intestinal mucosal permeability,increased,while serum lipopolysaccharide and D-lactate levels decreased.Fecal metagenomic analysis indicated that therapeutic effects of L.reuteri FN041 may be microbiota-dependent,demonstrated by increased beneficial gut bacteria and decreased bacteria associated with inflammation and tissue damage.Metabolomic analysis of cecal contents revealed that 1-myristoyl-sn-glycero-3-phosphocholine,4-androsten-17-beta-ol-3-one glucosiduronate,gamma-L-glutamylputrescine,fosfomycin and alpha-cyperone were metabolite markers significantly disrupted by DSS and partially restored by probiotics.Correlation analysis among clinical colitis parameters,metagenomics,and metabolomics confirmed the interdependence of these factors.Thus,L.reuteri FN041 may alleviate colitis by reshaping the intestinal microbial flora and metabolites.In summary,L.reuteri FN041 represents a promising probiotic for UC prevention and treatment.展开更多
基金supported by the National Natural Science Foundation of China(22108188,U21A20303,U20A20141)CAS Project for Young Scientists in Basic Research(YSBR-039)。
文摘A nickel hexacyanoferrate(NiHCF)film electrode was prepared with NiHCF,conductive carbon black,and polyvinylidene difluoride,which was coated on graphite plate substrate for selective extraction of Cs^(+)ions by using electrochemically switched ion exchange(ESIX)technology.A potential-responsive ionpump system for efficient extraction of Cs+ions was designed,and the effect of wet film thicknesses,charging modes,flow rates,and chamber widths on Cs+ions extraction performance was investigated.In the system,the adsorption capacity and removal percentage of Cs^(+)ions on the NiHCF film electrode reached as high as 147.69 mg·g^(-1)and 92.47%,respectively.Furthermore,the NiHCF film electrode showed high selectivity for Cs^(+)ions and stability.After seven cycles of adsorption/desorption,the desorption percentage could reach about 100%.The excellent Cs^(+)extraction performance should be attributed to the strong driving force produced by the potential-responsive ion-pumping effect in the ESIX process,as well as the low ion transfer resistance of the film electrode which is caused by the special crystal structure of NiHCF.In addition,the NiHCF film electrode was implemented to work together with the bismuth oxybromide(BiOBr)film electrode to accomplish the simultaneous extraction of Cs^(+)and Br^(-).And the adsorption capacity and removal percentage of Br^(-)ions on the BiOBr film electrode reached 69.53 mg·g^(-1)and 77.32%,correspondingly.It is expected that such a potential-responsive ion-pump system based on NiHCF and BiOBr film electrodes could be used for the selective extraction and concentration of Cs^(+)and Br^(-)ions from salt lake brine.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61927813,61865009,and 12104203)Jiangxi Provincial Natural Science Foundation(Grant No.20212ACB201007).
文摘Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor.It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip.For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment,dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001μg·ml-1 and to 0.01μg·ml-1,respectively.Furthermore,when aflatoxin B1 concentrations are 0.1μg·ml-1,1μg·ml-1,10μg·ml-1 and 100μg·ml-1,dip amplitudes of 0.2179,0.226,0.2384 and 0.2527 and dip redshifts of 10.1 GHz,20.1 GHz,27.7 GHz and 37.6 GHz are respectively observed.These results illustrate high-sensitivity,label-free detection of aflatoxin B1,enriching the applications of sensors in the terahertz domain.
基金supported by the Jiangsu Provincial Department of Science and Technology(No.BE2022698)the Wuxi Municipal Science and Technology Bureau(No.Y20222003)+2 种基金the Medical Key Discipline Program of Wuxi Health Commission(No.CXTD202113)the Top Talent Support Program for young and middle-aged people of Wuxi Health Commission(No.BJ2023077)the Biobank Program of Wuxi Health Commission(No.SW202201).
文摘Food allergy is an emerging global health concern with limited treatment options.This study evaluated the efficacy of Limosilactobacillus reuteri(L.reuteri)FN041 in a cow’s milkβ-lactoglobulin-induced murine model of food allergy.Daily oral administration of L.reuteri FN041 alleviated allergic symptoms,normalized body temperature,and reduced lung inflammation.L.reuteri FN041 lowered serum IgE levels,rebalanced Th1/Th2 cytokines,and expanded splenic regulatory T cells.Intestinal barrier integrity was improved,as evidenced by restored tight junction proteins and enhanced antioxidant capacity.Although the overall gut microbialα-andβ-diversity remained unchanged,L.reuteri FN041 selectively enriched Duncaniella muris and Muribaculum gor-doncarteri,while suppressing Waltera intestinalis and Lacrimispora xylanisolvens.Metabolomic profiling revealed an upregulation of anti-inflammatory flavonoids,particularly naringenin-7-O-glucoside.Mechanistically,L.reuteri FN041 inhibited AKT/mTOR and NF-κB signaling.These findings suggest that L.reuteri FN041 is a promising biotherapeutic for food allergy,acting through immune modulation,gut barrier protection,microbiota remodeling,and oxidative stress mitigation.
基金supported by the Jiangsu Provincial Department of Science and Technology(No.BE2022698)the Wuxi Municipal Science and Technology Bureau(No.Y20222003)+2 种基金Medical Key Discipline Program of Wuxi Health Commission(No.CXTD202113)the Top Talent Support Program for young and middle-aged people of Wuxi Health Commission(No.BJ2023077)the Biobank Program of Wuxi Health Commission(No.SW202201).
文摘Limosilactobacillus reuteri(L.reuteri)FN041,a probiotic isolated from breast milk,exhibits immunomodulatory effects against allergic and immune-related diseases.However,the preventive and therapeutic potential of L.reuteri FN041 in ulcerative colitis(UC)remains unreported.This study investigates the protective effects of L.reuteri FN041 in a mouse model of colitis induced by dextran sodium sulfate(DSS).Significant alleviation of colitis symptoms was observed with L.reuteri FN041 treatment,demonstrated by decreased weight loss,reduced colon shortening,and lower Disease Activity Index and Histological Index scores.Inflammation and oxidative stress levels were reduced,as shown by lower local and systemic IL-6,elevated IL-10 and increased colonic malondialdehyde levels.The expression of tight junction proteins,indicators of intestinal mucosal permeability,increased,while serum lipopolysaccharide and D-lactate levels decreased.Fecal metagenomic analysis indicated that therapeutic effects of L.reuteri FN041 may be microbiota-dependent,demonstrated by increased beneficial gut bacteria and decreased bacteria associated with inflammation and tissue damage.Metabolomic analysis of cecal contents revealed that 1-myristoyl-sn-glycero-3-phosphocholine,4-androsten-17-beta-ol-3-one glucosiduronate,gamma-L-glutamylputrescine,fosfomycin and alpha-cyperone were metabolite markers significantly disrupted by DSS and partially restored by probiotics.Correlation analysis among clinical colitis parameters,metagenomics,and metabolomics confirmed the interdependence of these factors.Thus,L.reuteri FN041 may alleviate colitis by reshaping the intestinal microbial flora and metabolites.In summary,L.reuteri FN041 represents a promising probiotic for UC prevention and treatment.
