The effects of kojic acid on phenoloxidase (PO) of Plutella xylostella were investigated, when it had been partially purified by 40% saturated [(NH4)2SO4] and Sephadex G-100 gel filtration. Kojic acid showed inhibit...The effects of kojic acid on phenoloxidase (PO) of Plutella xylostella were investigated, when it had been partially purified by 40% saturated [(NH4)2SO4] and Sephadex G-100 gel filtration. Kojic acid showed inhibitory effects on both monophenolase and o-diphenolase activity of the PO. The inhibitor concentrations leading to 50% (IC50) activity lost were estimated to be 0.07 mmol L-1 for monophenolase and 1 mmol L-1 for diphenolase, respectively. Kojic acid can also prolong the lag time of PO for oxidation of L-tyrosine. The inhibition kinetics were analyzed by Lineweaver-Burk plots and kojic acid was found to be a reversible competitive inhibitor with the Ki of 0.47 mmol L-1. The ability of kojic acid to inhibit the enzyme activity may be associated with its ability to chelate copper at the active site. In addition, the iron ion was found to shorten the lag time obviously, but have no important effect on the monophenolase activity.展开更多
As an eco-economic tree species in the three northern regions, Prunus armeniaca has the characteristics of strong adaptability, wide distribution, high resistance and high survival rate. It is usually used as a wastel...As an eco-economic tree species in the three northern regions, Prunus armeniaca has the characteristics of strong adaptability, wide distribution, high resistance and high survival rate. It is usually used as a wasteland afforestation priority. In order to provide technical support and reference for the ecological construction of barren hills and wasteland, this paper firstly introduces the sowing and breeding technology of apricot and the cloning and breeding technology of apricot, and then introduces the seedling raising technology, seed quality identification, sowing and management and nursery of apricot from the aspects of soil preparation, seedling raising and seedling raising.展开更多
In recent years, due to the progress of national economy, people's living standard is also getting higher and higher. However, China's forestry industry is also facing many problems in the process of rapid and...In recent years, due to the progress of national economy, people's living standard is also getting higher and higher. However, China's forestry industry is also facing many problems in the process of rapid and vigorous development, delaying the country's economic and social vigorous development rate. Moreover, the social problems in forestry development are not conducive to the formulation of sustainable economic development strategies. As the seedling raising technology of modern forestry is a systematic technology, the requirements for technicians are also very high. In the modern forestry seedling raising project, seedling raising, afforestation and other activities are considered. Only by further making up for seedling cultivation techniques and afforestation methods can the project meet the needs of the vigorous development of modern forestry projects. Based on this, the requirements of modern forestry seedling raising engineering technology and afforestation plan will be analyzed for reference.展开更多
基金The study was supperted by the State Science and Technology Commission of China(30270887).
文摘The effects of kojic acid on phenoloxidase (PO) of Plutella xylostella were investigated, when it had been partially purified by 40% saturated [(NH4)2SO4] and Sephadex G-100 gel filtration. Kojic acid showed inhibitory effects on both monophenolase and o-diphenolase activity of the PO. The inhibitor concentrations leading to 50% (IC50) activity lost were estimated to be 0.07 mmol L-1 for monophenolase and 1 mmol L-1 for diphenolase, respectively. Kojic acid can also prolong the lag time of PO for oxidation of L-tyrosine. The inhibition kinetics were analyzed by Lineweaver-Burk plots and kojic acid was found to be a reversible competitive inhibitor with the Ki of 0.47 mmol L-1. The ability of kojic acid to inhibit the enzyme activity may be associated with its ability to chelate copper at the active site. In addition, the iron ion was found to shorten the lag time obviously, but have no important effect on the monophenolase activity.
文摘As an eco-economic tree species in the three northern regions, Prunus armeniaca has the characteristics of strong adaptability, wide distribution, high resistance and high survival rate. It is usually used as a wasteland afforestation priority. In order to provide technical support and reference for the ecological construction of barren hills and wasteland, this paper firstly introduces the sowing and breeding technology of apricot and the cloning and breeding technology of apricot, and then introduces the seedling raising technology, seed quality identification, sowing and management and nursery of apricot from the aspects of soil preparation, seedling raising and seedling raising.
文摘In recent years, due to the progress of national economy, people's living standard is also getting higher and higher. However, China's forestry industry is also facing many problems in the process of rapid and vigorous development, delaying the country's economic and social vigorous development rate. Moreover, the social problems in forestry development are not conducive to the formulation of sustainable economic development strategies. As the seedling raising technology of modern forestry is a systematic technology, the requirements for technicians are also very high. In the modern forestry seedling raising project, seedling raising, afforestation and other activities are considered. Only by further making up for seedling cultivation techniques and afforestation methods can the project meet the needs of the vigorous development of modern forestry projects. Based on this, the requirements of modern forestry seedling raising engineering technology and afforestation plan will be analyzed for reference.
