TOPLESS/TOPLESS-RELATED(TPL/TPR)proteins are transcriptional corepressors that play pivotal roles in plant development,hormone signaling,and stress responses.Although TPL/TPR proteins have been identified in various o...TOPLESS/TOPLESS-RELATED(TPL/TPR)proteins are transcriptional corepressors that play pivotal roles in plant development,hormone signaling,and stress responses.Although TPL/TPR proteins have been identified in various organisms,their functions in rice disease resistance remain largely unexplored.Here,we conducted a comprehensive analysis of the three rice TPL/TPR proteins,designated OsTPR1,OsTPR2,and OsTPR3,examining their evolutionary relationships,expression patterns,and subcellular localization,and assessing their roles in disease resistance.Phylogenetic analysis revealed that the three OsTPRs belonged to distinct evolutionary clades.Expression analysis demonstrated tissue-specific patterns and responsiveness to jasmonate(JA),with all three genes being induced upon infection with Xanthomonas oryzae pv.oryzae(Xoo).Consistent with their roles as transcriptional corepressors,all three OsTPRs localized to the nucleus.Disease resistance assays showed that,after inoculation with Xoo,lesion lengths on ostpr2 and ostpr3 mutants were significantly shorter than those on wild-type plants.Protein interaction assays demonstrated that OsTPR2 interacted with JA ZIM-domain protein(OsJAZ12),whose expression is also induced by Xoo.Furthermore,haplotype analysis of OsTPRs revealed natural variation,leading to the identification of superior allelic variants that confer improved resistance to bacterial blight without a yield penalty.Collectively,our findings provide a systematic characterization of TPL/TPR proteins in rice,highlight their potential roles in resistance to bacterial leaf blight,and identify valuable allelic resources for molecular breeding aimed at improving both disease resistance and yield.展开更多
目的了解广州市男男性行为者(men who have sex with men,MSM)的抑郁状况并探讨其影响因素。方法2017-06/12采用方便抽样法,在广州市越秀区疾病预防控制中心和岭南伙伴社区支持中心招募MSM进行匿名式问卷调查,问卷内容主要包括人口学特...目的了解广州市男男性行为者(men who have sex with men,MSM)的抑郁状况并探讨其影响因素。方法2017-06/12采用方便抽样法,在广州市越秀区疾病预防控制中心和岭南伙伴社区支持中心招募MSM进行匿名式问卷调查,问卷内容主要包括人口学特征、性行为特征、Kinsey量表、流调中心用抑郁自评量表及儿童期虐待量表。利用R version 3.6.1进行数据分析,采用t检验、卡方检验和Wilcoxon秩和检验等进行单因素分析,采用Logistic回归进行多因素分析。结果共调查463名MSM,抑郁症状检出率为43.84%(203/463)。单因素分析结果显示,儿童期虐待量表总得分(t=5.398,P=0.000)以及分量表中情感虐待(t=6.095,P=0.000)、躯体虐待(t=3.249,P=0.001)、性虐待(t=2.879,P=0.004)、躯体忽视(t=2.034,P=0.042)和情感忽视(t=3.503,P=0.001)的得分均会影响MSM抑郁症状的检出率。多因素Logistic回归分析结果显示,儿童期虐待分量表中情感虐待得分较高是MSM抑郁症状发生的危险因素。结论广州市MSM的抑郁症状检出率较高,抑郁症状的发生与儿童期的情感虐待相关。今后应加强对MSM的心理干预。展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 32302291 and 32400207)the Zhejiang A&F University Research and Development Fund Project, China (Grant Nos. 2025LFR012 and 2022LFR127)
文摘TOPLESS/TOPLESS-RELATED(TPL/TPR)proteins are transcriptional corepressors that play pivotal roles in plant development,hormone signaling,and stress responses.Although TPL/TPR proteins have been identified in various organisms,their functions in rice disease resistance remain largely unexplored.Here,we conducted a comprehensive analysis of the three rice TPL/TPR proteins,designated OsTPR1,OsTPR2,and OsTPR3,examining their evolutionary relationships,expression patterns,and subcellular localization,and assessing their roles in disease resistance.Phylogenetic analysis revealed that the three OsTPRs belonged to distinct evolutionary clades.Expression analysis demonstrated tissue-specific patterns and responsiveness to jasmonate(JA),with all three genes being induced upon infection with Xanthomonas oryzae pv.oryzae(Xoo).Consistent with their roles as transcriptional corepressors,all three OsTPRs localized to the nucleus.Disease resistance assays showed that,after inoculation with Xoo,lesion lengths on ostpr2 and ostpr3 mutants were significantly shorter than those on wild-type plants.Protein interaction assays demonstrated that OsTPR2 interacted with JA ZIM-domain protein(OsJAZ12),whose expression is also induced by Xoo.Furthermore,haplotype analysis of OsTPRs revealed natural variation,leading to the identification of superior allelic variants that confer improved resistance to bacterial blight without a yield penalty.Collectively,our findings provide a systematic characterization of TPL/TPR proteins in rice,highlight their potential roles in resistance to bacterial leaf blight,and identify valuable allelic resources for molecular breeding aimed at improving both disease resistance and yield.
文摘目的了解广州市男男性行为者(men who have sex with men,MSM)的抑郁状况并探讨其影响因素。方法2017-06/12采用方便抽样法,在广州市越秀区疾病预防控制中心和岭南伙伴社区支持中心招募MSM进行匿名式问卷调查,问卷内容主要包括人口学特征、性行为特征、Kinsey量表、流调中心用抑郁自评量表及儿童期虐待量表。利用R version 3.6.1进行数据分析,采用t检验、卡方检验和Wilcoxon秩和检验等进行单因素分析,采用Logistic回归进行多因素分析。结果共调查463名MSM,抑郁症状检出率为43.84%(203/463)。单因素分析结果显示,儿童期虐待量表总得分(t=5.398,P=0.000)以及分量表中情感虐待(t=6.095,P=0.000)、躯体虐待(t=3.249,P=0.001)、性虐待(t=2.879,P=0.004)、躯体忽视(t=2.034,P=0.042)和情感忽视(t=3.503,P=0.001)的得分均会影响MSM抑郁症状的检出率。多因素Logistic回归分析结果显示,儿童期虐待分量表中情感虐待得分较高是MSM抑郁症状发生的危险因素。结论广州市MSM的抑郁症状检出率较高,抑郁症状的发生与儿童期的情感虐待相关。今后应加强对MSM的心理干预。
