Plant morphology is specified by leaves and flowers,and the shoot apical meristem(SAM)defines the architecture of plant leaves and flowers.Here,we reported the characterization of a soybean KNOX gene GmKNT1,which was ...Plant morphology is specified by leaves and flowers,and the shoot apical meristem(SAM)defines the architecture of plant leaves and flowers.Here,we reported the characterization of a soybean KNOX gene GmKNT1,which was highly homologous to Arabidopsis STM.The GmKNT1 was strongly expressed in roots,flowers and developing seeds.Its expression could be induced by IAA,ABA and JA,but inhibited by GA or cytokinin.Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region,SAM of young leaves,sepal and carpel,not in seed and mature leaves.Scanning electron microscopy(SEM)disclosed multiple changes in morphology of the epidermal cells and stigma.The transgenic Arabidopsis plants overexpressing the GmKNT1 showed small and lobed leaves,shortened internodes and small clustered inflorescence.The lobed leaves might result from the function of the meristems located at the boundary of the leaf.Compared with wild type plants,transgenic plants had higher expression of the SAM-related genes including the CUP,WUS,CUC1,KNAT2 and KNAT6.These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression.展开更多
Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with...Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with mercury film was used for simultaneous voltammetric detection of zinc,cadmium,lead and copper ions in water.The electrochemical behavior and the actual surface area of the MEA were investigated by cyclic voltammetry in K_(3)[Fe(CN)_(6)].Electrochemical impedance spectrum(EIS)was utilized to examine the deposition of mercury on the electrode surface.Based on anodic stripping voltammetry,mercury filmAu MEA was applied to the detection of heavy metals in artificial analyte,where good calibrate linearity was obtained for cadmium,lead and copper ions,but with zinc exhibiting poor linearity.展开更多
Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer auto...Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis.This study included the multiparameter cell-based biosensors,cell culture chamber,drug auto-injection detection and analysis.The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume.Finally,the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer,and study effects of different drugs on cell metabolism.Further,the research explores drug analysis method of the multiparameter microphysiometer.The results showed that the cell-based microphysiometer system provides a utility platform for rapid,long-term and automatic cell physiological environment detection and drug analysis.展开更多
The hydrogeologic conditions in Tianjin,China,are characterized by a system of multiple alternating aquifers and aquitards and a high groundwater level.Generally,the groundwater system to a depth of 70 m includes one ...The hydrogeologic conditions in Tianjin,China,are characterized by a system of multiple alternating aquifers and aquitards and a high groundwater level.Generally,the groundwater system to a depth of 70 m includes one phreatic aquifer and three semiconfined aquifers(AqI–AqIII).The distribution of aquifers varies greatly.Accurate estimates of aquifer parameters from pumping tests are essential in geotechnical engineering practice.In this study,the applicability of different models for estimating hydraulic parameters in a multiaquifer system is analyzed.To avoid errors in the graphic-analytical process and to enhance the efficiency and accuracy,a hybrid algorithm,called GALMA(a genetic algorithm(GA)combined with the Levenberg–Marquardt(LM)algorithm),is used with the Neuman and Witherspoon model and ratio method.The estimated values in the second and third semiconfined aquifers(AqII and AqIII)from various solutions are compared with the measured data.Further validations based on the prediction of the drawdown values are performed via a three dimensional numerical simulation.展开更多
基金supported by the National Natural Science Foundation of China(No.30671316)the National Basic Research Program of China(No.2002CB 111303).
文摘Plant morphology is specified by leaves and flowers,and the shoot apical meristem(SAM)defines the architecture of plant leaves and flowers.Here,we reported the characterization of a soybean KNOX gene GmKNT1,which was highly homologous to Arabidopsis STM.The GmKNT1 was strongly expressed in roots,flowers and developing seeds.Its expression could be induced by IAA,ABA and JA,but inhibited by GA or cytokinin.Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region,SAM of young leaves,sepal and carpel,not in seed and mature leaves.Scanning electron microscopy(SEM)disclosed multiple changes in morphology of the epidermal cells and stigma.The transgenic Arabidopsis plants overexpressing the GmKNT1 showed small and lobed leaves,shortened internodes and small clustered inflorescence.The lobed leaves might result from the function of the meristems located at the boundary of the leaf.Compared with wild type plants,transgenic plants had higher expression of the SAM-related genes including the CUP,WUS,CUC1,KNAT2 and KNAT6.These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression.
基金This work has been supported by grants from the National Basic Research Program of China(973 program),Grant No.2009CB320303.
文摘Qualitative and quantitative analysis of trace heavy metals in aqueous environment are rapidly assuming significance along with the rapid development of industry.In this paper,gold microelectrode array(MEA)plated with mercury film was used for simultaneous voltammetric detection of zinc,cadmium,lead and copper ions in water.The electrochemical behavior and the actual surface area of the MEA were investigated by cyclic voltammetry in K_(3)[Fe(CN)_(6)].Electrochemical impedance spectrum(EIS)was utilized to examine the deposition of mercury on the electrode surface.Based on anodic stripping voltammetry,mercury filmAu MEA was applied to the detection of heavy metals in artificial analyte,where good calibrate linearity was obtained for cadmium,lead and copper ions,but with zinc exhibiting poor linearity.
基金This work was supported by the National Natural Science Foundation of China(Grant No.81027003)the Zhejiang Provincial Science and Technology Major Special Program(No.2010C14006).
文摘Cellular metabolism arouses the changes of substance in extracellular physiological microenvironment,and the metabolic level reflects the physiological state of cells.This paper developed a novel microphysiometer automatic analysis instrument based on multiparameter cell-based biosensors for quick drug analysis.This study included the multiparameter cell-based biosensors,cell culture chamber,drug auto-injection detection and analysis.The analysis instrument was capable of real-time detection for the acidic product and other chemical parameters generated by the cellular metabolism in the micro-volume.Finally,the paper employs human breast cancer cell line MCF-7 and drug experiments to verify the performance of microphysiometer,and study effects of different drugs on cell metabolism.Further,the research explores drug analysis method of the multiparameter microphysiometer.The results showed that the cell-based microphysiometer system provides a utility platform for rapid,long-term and automatic cell physiological environment detection and drug analysis.
基金This research was supported by the National Natural Science Foundation of China(Nos.51708405,51378345)the Project of Tianjin Science and Technology Plan(No.16YDLJSF00040).
文摘The hydrogeologic conditions in Tianjin,China,are characterized by a system of multiple alternating aquifers and aquitards and a high groundwater level.Generally,the groundwater system to a depth of 70 m includes one phreatic aquifer and three semiconfined aquifers(AqI–AqIII).The distribution of aquifers varies greatly.Accurate estimates of aquifer parameters from pumping tests are essential in geotechnical engineering practice.In this study,the applicability of different models for estimating hydraulic parameters in a multiaquifer system is analyzed.To avoid errors in the graphic-analytical process and to enhance the efficiency and accuracy,a hybrid algorithm,called GALMA(a genetic algorithm(GA)combined with the Levenberg–Marquardt(LM)algorithm),is used with the Neuman and Witherspoon model and ratio method.The estimated values in the second and third semiconfined aquifers(AqII and AqIII)from various solutions are compared with the measured data.Further validations based on the prediction of the drawdown values are performed via a three dimensional numerical simulation.
