Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of root...Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of rootstock in grafted watermelon(Citrullus lanatus)seedlings.In this study,the effects of different light treatments on root regeneration were determined.This revealed that addition of far-red light(Fr)could significantly expedite root formation in the rootstock.Moreover,the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr,especially for auxin-response proteins(including CmIAA11,CmIAA17,and CmAUX28),Small auxinup RNA genes(including CmSAUR20 and CmSAUR50)and the auxin efflux transporter(CmPIN3).In addition,the expression of Phytochrome Interacting Factor(PIFs),such as CmPIF1,CmPIF3 and CmPIF7,was remarkably increased by Fr.These genes may act together to activate auxinrelated pathways under Fr treatment.Based on the results of HPLC-MS/MS analysis,the concentrations of different auxin-types in adventitious root were significantly influenced by Fr.Furthermore,the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment,which was determined by the acid magenta dyeing experiment.Therefore,all the results suggested that Fr could induce AR formation in rootstocks,which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes.The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings,which will provide a theoretical basis for the speeding development of industrialized seedlings production.展开更多
Solar greenhouses have been widely developed in China.Active heat storage walls using air ducts arranged in the walls can improve the walls'thermal performance and indoor temperatures of solar greenhouses.In the p...Solar greenhouses have been widely developed in China.Active heat storage walls using air ducts arranged in the walls can improve the walls'thermal performance and indoor temperatures of solar greenhouses.In the present work,three kinds of air duct layouts,namely straight-up-and-down duct(Z),fork-shaped top-in-bottom-out distribution duct(DF),and“±”-shaped top-in-side-out distribution duct(CF)are designed.The effect of the three air duct layouts on the heat transfer and storage characteristics of the back wall is studied using the computational fluid dynamics(CFD)method.Results show that after the same time period,the transferred heat amount in the back wall with duct DF is the largest,while that with duct CF is slightly greater than that with duct Z.The temperature of the back walls with air ducts is higher than that without air ducts.The air duct DF is the optimal among the three kinds of air duct layouts.The greenhouse with the duct DF in the back wall shows the most obviously increased indoor average temperature,the highest temperature at night,and the most uniform temperature.展开更多
基金supported by Jiangsu Provincial Basic Research Program(Natural Science Foundation,Grant No.BK20241175)The project of Agriculture Ministry Key Laboratory of Agricultural Engineering in the Middle and Lower Reaches of Yangtze River[Grant No.(23)3104]Jiangsu Agricultural Science and Technology Innovation Fund[Grant No.CX(21)2022]。
文摘Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of rootstock in grafted watermelon(Citrullus lanatus)seedlings.In this study,the effects of different light treatments on root regeneration were determined.This revealed that addition of far-red light(Fr)could significantly expedite root formation in the rootstock.Moreover,the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr,especially for auxin-response proteins(including CmIAA11,CmIAA17,and CmAUX28),Small auxinup RNA genes(including CmSAUR20 and CmSAUR50)and the auxin efflux transporter(CmPIN3).In addition,the expression of Phytochrome Interacting Factor(PIFs),such as CmPIF1,CmPIF3 and CmPIF7,was remarkably increased by Fr.These genes may act together to activate auxinrelated pathways under Fr treatment.Based on the results of HPLC-MS/MS analysis,the concentrations of different auxin-types in adventitious root were significantly influenced by Fr.Furthermore,the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment,which was determined by the acid magenta dyeing experiment.Therefore,all the results suggested that Fr could induce AR formation in rootstocks,which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes.The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings,which will provide a theoretical basis for the speeding development of industrialized seedlings production.
基金financially supported by the National Natural Science Foundation of China(31901420)Jiangsu Provincial Natural Science Foundation(Grant No.BK20241174)Key Research and Development Program Project of Ningxia Hui Autonomous Region(2023BCF01022).
文摘Solar greenhouses have been widely developed in China.Active heat storage walls using air ducts arranged in the walls can improve the walls'thermal performance and indoor temperatures of solar greenhouses.In the present work,three kinds of air duct layouts,namely straight-up-and-down duct(Z),fork-shaped top-in-bottom-out distribution duct(DF),and“±”-shaped top-in-side-out distribution duct(CF)are designed.The effect of the three air duct layouts on the heat transfer and storage characteristics of the back wall is studied using the computational fluid dynamics(CFD)method.Results show that after the same time period,the transferred heat amount in the back wall with duct DF is the largest,while that with duct CF is slightly greater than that with duct Z.The temperature of the back walls with air ducts is higher than that without air ducts.The air duct DF is the optimal among the three kinds of air duct layouts.The greenhouse with the duct DF in the back wall shows the most obviously increased indoor average temperature,the highest temperature at night,and the most uniform temperature.
