Liver cancer is a major killer threatening human health worldwide.At this stage the clinical choice to the treatment of liver cancer is a combination of surgery,chemotherapy and radiotherapy.Alternatively,using hydrog...Liver cancer is a major killer threatening human health worldwide.At this stage the clinical choice to the treatment of liver cancer is a combination of surgery,chemotherapy and radiotherapy.Alternatively,using hydrogen to treat cancer has great prospects and development space.Herein,we fabricated a hierarchical and flexible electrode that being able to continuously generate hydrogen in vivo in the deep abdominal liver through efficient water electrolysis to kill tumor cells and regulate the tumor microenvironment.The flexibility of the electrode facilitated to fit the tumor surface and thus improved the contact area of hydrogen therapy.By in situ growth of molybdenum disulfide on a hierarchical carbon skeleton,improved reaction kinetics and smaller impedance with a low overpotential of 1.02 V at-10 m A/cm^(2)in cell culture medium and Tafel slope of 73 m V/dec were achieved.Animal experiments showed that the electrode could effectively inhibit the growth of human hepatocellular carcinoma cells in nude mice by efficient H_(2)-production in vivo.The apoptosis rate of cancer cells reached 81.8%,and the proliferation rate decreased to 3.39%.Moreover,this treatment does not affect the growth of normal hepatocytes according to the results of cell experiments.This study demonstrated that the in vivo hydrogen production by our flexible electrode is a safe and effective treatment for liver cancer,with the advantages of minimal invasiveness,simple operation,low side effects and low cost.展开更多
基金financially supported by research grants from the Natural Science Foundation of China(No.52173235)Science and Technology Innovation and Improving Project of Army Medical University(No.2021XJS24)+1 种基金Science and Technology Innovation Capability Enhancement Project of Army Medical University(No.2022XJS20)Key Innovation Project for Clinical Technology of the Second Affiliated Hospital of Army Medical University(No.2018JSLC0025)。
文摘Liver cancer is a major killer threatening human health worldwide.At this stage the clinical choice to the treatment of liver cancer is a combination of surgery,chemotherapy and radiotherapy.Alternatively,using hydrogen to treat cancer has great prospects and development space.Herein,we fabricated a hierarchical and flexible electrode that being able to continuously generate hydrogen in vivo in the deep abdominal liver through efficient water electrolysis to kill tumor cells and regulate the tumor microenvironment.The flexibility of the electrode facilitated to fit the tumor surface and thus improved the contact area of hydrogen therapy.By in situ growth of molybdenum disulfide on a hierarchical carbon skeleton,improved reaction kinetics and smaller impedance with a low overpotential of 1.02 V at-10 m A/cm^(2)in cell culture medium and Tafel slope of 73 m V/dec were achieved.Animal experiments showed that the electrode could effectively inhibit the growth of human hepatocellular carcinoma cells in nude mice by efficient H_(2)-production in vivo.The apoptosis rate of cancer cells reached 81.8%,and the proliferation rate decreased to 3.39%.Moreover,this treatment does not affect the growth of normal hepatocytes according to the results of cell experiments.This study demonstrated that the in vivo hydrogen production by our flexible electrode is a safe and effective treatment for liver cancer,with the advantages of minimal invasiveness,simple operation,low side effects and low cost.
