Wheat stripe rust is a devastating disease in many regions of the world.In wheat,49 resistance genes for stripe rust have been officially documented,but only three genes are cloned,including the race-specific resistan...Wheat stripe rust is a devastating disease in many regions of the world.In wheat,49 resistance genes for stripe rust have been officially documented,but only three genes are cloned,including the race-specific resistance YrlO candidate gene(YrlOca)and slow-rusting genes Lr34/Yr18(hereafter designated as Yrl8)and Fr36.In this study,we developed gene-specific markers for these genes and used them to screen a collection of 659 wheat accessions,including 485 Chinese cultivars.Thirteen percent and eleven percent of the tested Chinese cultivars were positive for the markers for YrlOca and Yr18nn(the resistant haplotype of YrlS),respectively,but none were positive for the Yr36 marker.Since there is a limited use of the YrlO gene in Chinese wheat,the relatively high frequency of wheat varieties with the YrlOcG marker suggests that the identity of the YrlO gene is unknown.With regards to the Yr18 gene,29%of the tested cultivars that are used in the Middle and Lower Yangtze Valleys'winter wheat zone were positive for Yrl8RH markers.A non-functional allele of Yrl8Rn was identified in'Mingxian 169',a commonly used susceptible check for studying stripe rust.The data presented here will provide useful information for marker-assisted selection for wheat stripe rust resistance.展开更多
Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites.Seed-specific promoters(SSPs)can direct the expression of genes specifically in seeds to avoid undesirable...Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites.Seed-specific promoters(SSPs)can direct the expression of genes specifically in seeds to avoid undesirable effects associated with constitutive expression.However,few SSPs have been identified in peanut.Previous studies have shown that some allergen-encoding genes encode seed storage proteins or exhibit seed-specific/preferential expression.In this study,we characterized allergen-encoding genes from across the genomes of Arachis species to explore seed-specific genes.We found that at least 9 out of 16 identified peanut allergen-encoding genes were expressed specifically in the seeds or were preferentially expressed.A 1493-bp promoter fragment of allergen gene Ara h 1(we named it AHSSP6)was isolated from cultivated peanut genome.cis-element analysis showed that three RY repeat elements which usually exsisted in seed or embryo specific promoter sequence were also present in AHSSP6 sequence.Histochemical analysis showed AHSSP6 could drive the expression of aβ-glucuronidase(GUS)reporter gene specifically in the seeds or cotyledon tissue of transgenic Arabidopsis,while not in other tissues.These findings indicated that these promoters of allergen genes were candidate SSPs,and AHSSP6 was a novel SSP which could be potentially utilized in peanut improvement.展开更多
Many species of Triticeae display a glaucous phenotype. In wheat, glaucousness/waxiness on spikes, leaves and shoots is controlled by wax production genes(W loci) and epistatic inhibitors(Iw loci). In this study, a su...Many species of Triticeae display a glaucous phenotype. In wheat, glaucousness/waxiness on spikes, leaves and shoots is controlled by wax production genes(W loci) and epistatic inhibitors(Iw loci). In this study, a suppressor of glaucousness from wild emmer wheat(Triticum turgidum ssp. dicoccoides) accession "PI 481521" was investigated in a pair of durum(T. turgidum ssp. durum cv. "Langdon", LDN)—wild emmer wheat chromosome substitution lines, LDN and "LDNDIC521-2B". Genetic analysis revealed that the non-glaucous phenotype of LDNDIC521-2Bwas controlled by the dominant glaucous suppressor Iw1 on the short arm of chromosome 2B. In total, 371 2B-specific marker differences were identified between LDN and LDNDIC521-2B. The location of the Iw1 gene was mapped using an F2 population that stemmed from LDN and LDNDIC521-2B, generating a partial linkage map that included 19 simple sequence repeats(SSR) and ten gene-based markers. On the current map, the Iw1 gene was located within the Xgwm614–BE498111 interval, and cosegregated with BQ788707,CD893659, CD927782, CD938589, and Xbarc35. Mapping of Iw1 in LDNDIC521-2B, a publically accessible and widely distributed line, will provide valuable information for marker-assisted selection of the agronomically important trait of glaucousness.展开更多
基金supported by the China Research and Development Initiative on Genetically Modified Plants(Grant No.2009ZX08009-053B)the National Basic Research Program of China(973 Program,Grant No.2011CB 100700)the National Natural Science Foundation of China(Grant No.30871323)
文摘Wheat stripe rust is a devastating disease in many regions of the world.In wheat,49 resistance genes for stripe rust have been officially documented,but only three genes are cloned,including the race-specific resistance YrlO candidate gene(YrlOca)and slow-rusting genes Lr34/Yr18(hereafter designated as Yrl8)and Fr36.In this study,we developed gene-specific markers for these genes and used them to screen a collection of 659 wheat accessions,including 485 Chinese cultivars.Thirteen percent and eleven percent of the tested Chinese cultivars were positive for the markers for YrlOca and Yr18nn(the resistant haplotype of YrlS),respectively,but none were positive for the Yr36 marker.Since there is a limited use of the YrlO gene in Chinese wheat,the relatively high frequency of wheat varieties with the YrlOcG marker suggests that the identity of the YrlO gene is unknown.With regards to the Yr18 gene,29%of the tested cultivars that are used in the Middle and Lower Yangtze Valleys'winter wheat zone were positive for Yrl8RH markers.A non-functional allele of Yrl8Rn was identified in'Mingxian 169',a commonly used susceptible check for studying stripe rust.The data presented here will provide useful information for marker-assisted selection for wheat stripe rust resistance.
基金funded by the Natural Science Foundation of Shandong Province(ZR2021MC128)National Natural Science Foundation of China(32001585)+1 种基金Shandong Elite Variety Project(2020LZGC001)Agro-industry Technology Research System of Shandong Province(SDAIT-04-02)。
文摘Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites.Seed-specific promoters(SSPs)can direct the expression of genes specifically in seeds to avoid undesirable effects associated with constitutive expression.However,few SSPs have been identified in peanut.Previous studies have shown that some allergen-encoding genes encode seed storage proteins or exhibit seed-specific/preferential expression.In this study,we characterized allergen-encoding genes from across the genomes of Arachis species to explore seed-specific genes.We found that at least 9 out of 16 identified peanut allergen-encoding genes were expressed specifically in the seeds or were preferentially expressed.A 1493-bp promoter fragment of allergen gene Ara h 1(we named it AHSSP6)was isolated from cultivated peanut genome.cis-element analysis showed that three RY repeat elements which usually exsisted in seed or embryo specific promoter sequence were also present in AHSSP6 sequence.Histochemical analysis showed AHSSP6 could drive the expression of aβ-glucuronidase(GUS)reporter gene specifically in the seeds or cotyledon tissue of transgenic Arabidopsis,while not in other tissues.These findings indicated that these promoters of allergen genes were candidate SSPs,and AHSSP6 was a novel SSP which could be potentially utilized in peanut improvement.
基金supported by the Natural Science Foundation of Shandong Province, China (JQ201107)the National Natural Science Foundation of China (31110103917)the Cooperative Innovation Center of Efficient Production with High Annual Yield of Wheat and Corn, Shandong Province, China
文摘Many species of Triticeae display a glaucous phenotype. In wheat, glaucousness/waxiness on spikes, leaves and shoots is controlled by wax production genes(W loci) and epistatic inhibitors(Iw loci). In this study, a suppressor of glaucousness from wild emmer wheat(Triticum turgidum ssp. dicoccoides) accession "PI 481521" was investigated in a pair of durum(T. turgidum ssp. durum cv. "Langdon", LDN)—wild emmer wheat chromosome substitution lines, LDN and "LDNDIC521-2B". Genetic analysis revealed that the non-glaucous phenotype of LDNDIC521-2Bwas controlled by the dominant glaucous suppressor Iw1 on the short arm of chromosome 2B. In total, 371 2B-specific marker differences were identified between LDN and LDNDIC521-2B. The location of the Iw1 gene was mapped using an F2 population that stemmed from LDN and LDNDIC521-2B, generating a partial linkage map that included 19 simple sequence repeats(SSR) and ten gene-based markers. On the current map, the Iw1 gene was located within the Xgwm614–BE498111 interval, and cosegregated with BQ788707,CD893659, CD927782, CD938589, and Xbarc35. Mapping of Iw1 in LDNDIC521-2B, a publically accessible and widely distributed line, will provide valuable information for marker-assisted selection of the agronomically important trait of glaucousness.
