Soybean(Glycine max)provides vegetable oils and proteins for human consumption.Its production depends on seeds and other productionrelated agronomic traits.How the seed traits are regulated in soybean remains largely ...Soybean(Glycine max)provides vegetable oils and proteins for human consumption.Its production depends on seeds and other productionrelated agronomic traits.How the seed traits are regulated in soybean remains largely unclear.In this study,we identified a miR172a-ERF416/413module for the regulation of seed traits.The miR172a can cleave the targets ERF416 and ERF413 to affect the downstream gene expression for the reduction of soybean seed size and weight.Both the MIR172a-overexpressing transgenic soybean plants and the erf416/413mutants produced smaller seeds than the control.Consistently,the ERF416-overexpressing transgenic soybean plants generated larger seeds.ERF416 and ERF413 were directly targeted to the promoter of Gm KIX8-1 and Gm SWEET10a to regulate their gene expression for seed size/weight control.Interestingly,the erf416/413mutants showed higher seed yield per plant and higher total seed fatty acid(FA)content,whereas the MIR172a-transgenic soybean had lower total seed FA content compared with the control cultivar,suggesting that mi R172a and ERF416/413 may function in FA accumulation through different pathways.Haplotypes of the ERF416 promoter region were further analyzed and Hap1 was correlated with higher gene expression and higher seed weight,while Hap3 was correlated with higher total seed lipid content.Our study revealed a new module for seed trait control.Manipulation of such alleles should facilitate breeding for high-oil and high-yield soybean cultivars.展开更多
Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and character...Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. Manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.展开更多
Ethylene is a gaseous hormone which plays important roles in both plant growth and development and stress responses. Based on studies in the dicot model plant species Arabidopsis, a linear ethylene signaling pathway h...Ethylene is a gaseous hormone which plays important roles in both plant growth and development and stress responses. Based on studies in the dicot model plant species Arabidopsis, a linear ethylene signaling pathway has been established,according to which ethylene is perceived by ethylene receptors and transduced through CONSTITUTIVE TRIPLE RESPONSE 1(CTR1) and ETHYLENE-INSENSITIVE 2(EIN2) to activate transcriptional reprogramming. In addition to this canonical signaling pathway, an alternative ethylene receptor-mediated phosphor-relay pathway has also been proposed to participate in ethylene signaling. In contrast to Arabidopsis, rice, a monocot, grows in semiaquatic environments and has a distinct plant structure. Several novel regulators and/or mechanisms of the rice ethylene signaling pathway have recently been identified,indicating that the ethylene signaling pathway in rice has its own unique features. In this review,we summarize the latest progress and compare the conserved and divergent aspects of the ethylene signaling pathway between Arabidopsis and rice. The crosstalk between ethylene and other plant hormones is also reviewed. Finally, we discuss how ethylene regulates plant growth, stress responses and agronomic traits. These analyses should help expand our knowledge of the ethylene signaling mechanism and could further be applied for agricultural purposes.展开更多
Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protei...Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protein Gm ZF351 is an oil level regulator. In this study, we discovered that the Gm ZF351 gene is induced by stress and that the overexpression of Gm ZF351 confers stress tolerance to transgenic soybean. Gm ZF351 directly regulates the expression of Gm CIPK9 and Gm SnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements.Stress induction of Gm ZF351 is mediated through reduction in the H3K27me3 level at the Gm ZF351locus.TwoJMJ30-demethylase-likegenes,Gm JMJ30-1 and Gm JMJ30-2, are involved in this demethylationprocess.Overexpressionof Gm JMJ30-1/2 in transgenic hairy roots enhances Gm ZF351 expression mediated by histone demethylation and confers stress tolerance to soybean.Yield-related agronomic traits were evaluated in stable Gm ZF351-transgenic plants under mild drought stress conditions. Our study reveals a new mode of Gm JMJ30-Gm ZF351 action in stress tolerance, in addition to that of Gm ZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments.展开更多
Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine...Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine max JASMONATEZIM DOMAIN 3(GmJAZ3),a gene identified through gene co-expression network analysis,regulates seed-related traits in soybean.Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation.GmJAZ3 interacted with both G.max RESPONSE REGULATOR 18a(GmRR18a)and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G.max CYTOKININ OXIDASE 3-4(GmCKX3-4),which usually affects seed traits.Meanwhile,the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression.In GmJAZ3-overexpressing soybean seeds,the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds,indicating that the GmJAZ3 affects seed size/weight and compositions.Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity,higher gene expression and higher seed weight.The Hap3 promoter may be selected and fixed during soybean domestication.JAZ3 orthologs from other plants/crops may also control seed size and weight.Taken together,our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control,providing promising targets during soybean molecular breeding for better seed traits.展开更多
基金supported by the National Natural Science Foundation of China(32090062,32090063,32171930)the National Key R&D Program(2021YFF1000104,2023YFD1200602)the Biological Breeding-National Science and Technology Major Project(2024ZD04078)。
文摘Soybean(Glycine max)provides vegetable oils and proteins for human consumption.Its production depends on seeds and other productionrelated agronomic traits.How the seed traits are regulated in soybean remains largely unclear.In this study,we identified a miR172a-ERF416/413module for the regulation of seed traits.The miR172a can cleave the targets ERF416 and ERF413 to affect the downstream gene expression for the reduction of soybean seed size and weight.Both the MIR172a-overexpressing transgenic soybean plants and the erf416/413mutants produced smaller seeds than the control.Consistently,the ERF416-overexpressing transgenic soybean plants generated larger seeds.ERF416 and ERF413 were directly targeted to the promoter of Gm KIX8-1 and Gm SWEET10a to regulate their gene expression for seed size/weight control.Interestingly,the erf416/413mutants showed higher seed yield per plant and higher total seed fatty acid(FA)content,whereas the MIR172a-transgenic soybean had lower total seed FA content compared with the control cultivar,suggesting that mi R172a and ERF416/413 may function in FA accumulation through different pathways.Haplotypes of the ERF416 promoter region were further analyzed and Hap1 was correlated with higher gene expression and higher seed weight,while Hap3 was correlated with higher total seed lipid content.Our study revealed a new module for seed trait control.Manipulation of such alleles should facilitate breeding for high-oil and high-yield soybean cultivars.
基金the National Natural Science Foundation of China，the National Key Basic Research Project，the National Transgenic Research Project
文摘Ethylene plays essential roles in adaptive growth of rice plants in water-saturating environment; how- ever, ethylene signaling pathway in rice is largely unclear. In this study, we report identification and characterization of ethylene-response mutants based on the specific ethylene-response phenotypes of etiolated rice seedlings, includ- ing ethylene-inhibited root growth and ethylene-promoted coleoptile elongation, which is different from the ethylene triple-response phenotype in Arabidopsis. We establish an efficient system for screening and a set of rice mutants have been identified. Genetic analysis reveals that these mutants form eight complementation groups. All the mutants show insensitivity or reduced sensitivity to ethylene in root growth but exhibit differential responses in cole0ptile growth. One mutant group mhz7 has insensitivity to ethylene in both root and coleoptile growth. We identified the corresponding gene by a map-based cloning method. MHZ7 encodes a membrane protein homologous to EIN2, a central component of ethylene signaling in Arabidopsis. Upon ethylene treatment, etiolated MHZ7-overexpressing seedlings exhibit enhanced coleoptiie elongation, increased mesocotyl growth and extremely twisted short roots, featuring enhanced ethylene- response phenotypes in rice. Grain length was promoted in MHZ7-transgenic plants and 1000-grain weight was reduced in mhz7 mutants. Leaf senescent process was also affected by MHZ7 expression. Manipulation of ethylene signaling may improve adaptive growth and yield-related traits in rice.
基金supported by the National Natural Science Foundation of China (31530004, 31670274, and 31600980)the State Key Lab of Plant Genomics。
文摘Ethylene is a gaseous hormone which plays important roles in both plant growth and development and stress responses. Based on studies in the dicot model plant species Arabidopsis, a linear ethylene signaling pathway has been established,according to which ethylene is perceived by ethylene receptors and transduced through CONSTITUTIVE TRIPLE RESPONSE 1(CTR1) and ETHYLENE-INSENSITIVE 2(EIN2) to activate transcriptional reprogramming. In addition to this canonical signaling pathway, an alternative ethylene receptor-mediated phosphor-relay pathway has also been proposed to participate in ethylene signaling. In contrast to Arabidopsis, rice, a monocot, grows in semiaquatic environments and has a distinct plant structure. Several novel regulators and/or mechanisms of the rice ethylene signaling pathway have recently been identified,indicating that the ethylene signaling pathway in rice has its own unique features. In this review,we summarize the latest progress and compare the conserved and divergent aspects of the ethylene signaling pathway between Arabidopsis and rice. The crosstalk between ethylene and other plant hormones is also reviewed. Finally, we discuss how ethylene regulates plant growth, stress responses and agronomic traits. These analyses should help expand our knowledge of the ethylene signaling mechanism and could further be applied for agricultural purposes.
基金supported by the National Natural Science Foundation of China (Grant Nos. U1906203, 32101676, 32171930, 31671258, 31971896)the Chinese Academy of Science (CAS) Key Project (ZDRW-ZS-2019-2)+1 种基金the Key R&D Project (Grant No. 2019YFD1002701)State Key Lab of Plant Genomics, IGDB, CAS。
文摘Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protein Gm ZF351 is an oil level regulator. In this study, we discovered that the Gm ZF351 gene is induced by stress and that the overexpression of Gm ZF351 confers stress tolerance to transgenic soybean. Gm ZF351 directly regulates the expression of Gm CIPK9 and Gm SnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements.Stress induction of Gm ZF351 is mediated through reduction in the H3K27me3 level at the Gm ZF351locus.TwoJMJ30-demethylase-likegenes,Gm JMJ30-1 and Gm JMJ30-2, are involved in this demethylationprocess.Overexpressionof Gm JMJ30-1/2 in transgenic hairy roots enhances Gm ZF351 expression mediated by histone demethylation and confers stress tolerance to soybean.Yield-related agronomic traits were evaluated in stable Gm ZF351-transgenic plants under mild drought stress conditions. Our study reveals a new mode of Gm JMJ30-Gm ZF351 action in stress tolerance, in addition to that of Gm ZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments.
基金supported by the National Key R&D Project(2021YFF1001201,2021YFF1000104-2)the National Natural Science Foundation of China(32090062,32090063)+2 种基金the Chinese Academy of Science leading project(XDA24010105)the State Key Lab of Plant Genomicsthe Qilu Zhongke Academy of Modern Microbiology Technology。
文摘Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine max JASMONATEZIM DOMAIN 3(GmJAZ3),a gene identified through gene co-expression network analysis,regulates seed-related traits in soybean.Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation.GmJAZ3 interacted with both G.max RESPONSE REGULATOR 18a(GmRR18a)and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G.max CYTOKININ OXIDASE 3-4(GmCKX3-4),which usually affects seed traits.Meanwhile,the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression.In GmJAZ3-overexpressing soybean seeds,the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds,indicating that the GmJAZ3 affects seed size/weight and compositions.Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity,higher gene expression and higher seed weight.The Hap3 promoter may be selected and fixed during soybean domestication.JAZ3 orthologs from other plants/crops may also control seed size and weight.Taken together,our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control,providing promising targets during soybean molecular breeding for better seed traits.
