Arabinogalactan from Lycium barbarum(LBP-3)is the major biological activity component and contributes to maintaining intestinal immune homeostasis by targeting gut microbiota and their metabolites.However,it is still ...Arabinogalactan from Lycium barbarum(LBP-3)is the major biological activity component and contributes to maintaining intestinal immune homeostasis by targeting gut microbiota and their metabolites.However,it is still a big challenge to know the specific ecological microbe-metabolite interaction regulated by LBP-3 due to the host complex diet webs and lots of unidentified intestinal bacteria.Therefore,the present study constructed a defined microbial consortium,composing of symbiotic Bacteroides caccae,Phocaeicola vulgatus,B.thetaiotaomicron,B.uniformis,B.ovatus,Lactobacillus plantarum and Escherichia coli in vitro,to investigate the impact of LBP-3 on the interactions of species and their metabolites.The utilization of LBP-3 by the defined microbial consortium of 7 intestinal species was 22.5%.Full-length 16S rRNA analysis demonstrated that LBP-3 significantly modulated the composition of synthetic bacterial community,especially inhibiting the level of opportunistic pathogenic E.coli and improving the relative abundance of probiotic L.plantarum and all Bacteroidetes species except B.ovatus.LBP-3 remarkedly influenced 27 differential metabolites,mainly belong to amino acids,peptides and analogues(37.04%)and carboxylic acid and derivatives(7.41%).Moreover,targeted metabolomics revealed that the levels of acetate,propionate,tryptophan and 5-methylthioadenosine were improved by LBP-3.In addition,LBP-3 modulated the pathways of tryptophan metabolism,cysteine and methionine metabolism,and biosynthesis of siderophore group nonribosomal peptides.Notably,some amino acids(e.g.,proline and glutamate)and their related metabolic pathways(e.g.,arginine and proline metabolism)altered by LBP-3 were significantly correlated with the levels of species,such as B.uniformis,P.vulgatus,and B.thetaiotaomicron.展开更多
基金supported by Natural Science Basic Research Program of Shaanxi(No.2024JC-YBQN-0238)Xi’an Association for Science and Technology Youth Talent Support Program(No.959202413057)+1 种基金Science and Technology Program of Xi’an(No.24NYGG0050)Shaanxi Provincial Science and Technology Key Project(No.2023-YBNY-167).
文摘Arabinogalactan from Lycium barbarum(LBP-3)is the major biological activity component and contributes to maintaining intestinal immune homeostasis by targeting gut microbiota and their metabolites.However,it is still a big challenge to know the specific ecological microbe-metabolite interaction regulated by LBP-3 due to the host complex diet webs and lots of unidentified intestinal bacteria.Therefore,the present study constructed a defined microbial consortium,composing of symbiotic Bacteroides caccae,Phocaeicola vulgatus,B.thetaiotaomicron,B.uniformis,B.ovatus,Lactobacillus plantarum and Escherichia coli in vitro,to investigate the impact of LBP-3 on the interactions of species and their metabolites.The utilization of LBP-3 by the defined microbial consortium of 7 intestinal species was 22.5%.Full-length 16S rRNA analysis demonstrated that LBP-3 significantly modulated the composition of synthetic bacterial community,especially inhibiting the level of opportunistic pathogenic E.coli and improving the relative abundance of probiotic L.plantarum and all Bacteroidetes species except B.ovatus.LBP-3 remarkedly influenced 27 differential metabolites,mainly belong to amino acids,peptides and analogues(37.04%)and carboxylic acid and derivatives(7.41%).Moreover,targeted metabolomics revealed that the levels of acetate,propionate,tryptophan and 5-methylthioadenosine were improved by LBP-3.In addition,LBP-3 modulated the pathways of tryptophan metabolism,cysteine and methionine metabolism,and biosynthesis of siderophore group nonribosomal peptides.Notably,some amino acids(e.g.,proline and glutamate)and their related metabolic pathways(e.g.,arginine and proline metabolism)altered by LBP-3 were significantly correlated with the levels of species,such as B.uniformis,P.vulgatus,and B.thetaiotaomicron.
