Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tusca...Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica(Italy)and sampled ca.60 days post-anthesis over three consecutive years(2016-2017-2018).We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics.To complement the data,a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids.Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola,while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties.Finally,proteomics on Morellona and Crognola showed variations in proteins involved in stress response,primary metabolism and cell wall expansion.To the best of our knowledge,this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes,proteins and metabolites.展开更多
Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,ca...Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,carotenoids and other antioxidants in plants.We determined the content and composition of main functional constituents of goji berries produced in central Italy in relation to the time of harvesting during two ripening seasons by analyzing the main environmental variables involved.The highest flavonoids content(0,19±0,006 mg QE/g)and antioxidant power(0,03±0,001 mmol Fe^(2+)eq/g)were found at the beginning of the fruiting season.In September,berries show the highest total polyphenols(3,22±0,09 mg GAE/g)and zeaxanthin contents(339,79±14,12μg/g).Our study focused on some important aspects related to the production of goji fruits with high nutraceutical characteristics in a non-experimental cultivation.Our results could provide reliable information on Goji performance in the European climate to be used for the exploitation of the species suggesting the best harvesting timing in terms of antioxidant power and nutraceutical features of berries.This information could be particularly useful for farmers eager to improve the quality of their products.展开更多
文摘Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica(Italy)and sampled ca.60 days post-anthesis over three consecutive years(2016-2017-2018).We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics.To complement the data,a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids.Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola,while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties.Finally,proteomics on Morellona and Crognola showed variations in proteins involved in stress response,primary metabolism and cell wall expansion.To the best of our knowledge,this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes,proteins and metabolites.
文摘Goji(Lycium barbarum L.)berries are well known for their biological activities and health-promoting antioxidant properties.Environmental and climatic factors can greatly affect accumulation of secondary metabolites,carotenoids and other antioxidants in plants.We determined the content and composition of main functional constituents of goji berries produced in central Italy in relation to the time of harvesting during two ripening seasons by analyzing the main environmental variables involved.The highest flavonoids content(0,19±0,006 mg QE/g)and antioxidant power(0,03±0,001 mmol Fe^(2+)eq/g)were found at the beginning of the fruiting season.In September,berries show the highest total polyphenols(3,22±0,09 mg GAE/g)and zeaxanthin contents(339,79±14,12μg/g).Our study focused on some important aspects related to the production of goji fruits with high nutraceutical characteristics in a non-experimental cultivation.Our results could provide reliable information on Goji performance in the European climate to be used for the exploitation of the species suggesting the best harvesting timing in terms of antioxidant power and nutraceutical features of berries.This information could be particularly useful for farmers eager to improve the quality of their products.
