ZSM-5 aggregates consisting of superfine and hierarchical nanocrystals(combined with micropores and intra-crystalline mesopores) with an average size of 30 nm were prepared through one-pot synthesis with the assistanc...ZSM-5 aggregates consisting of superfine and hierarchical nanocrystals(combined with micropores and intra-crystalline mesopores) with an average size of 30 nm were prepared through one-pot synthesis with the assistance of anionic polyacrylamide(APAM). The resultant zeolites(AHN-ZSM-5) were characterized by XRD, ICP-OES, SEM, TEM, BET, NH_3-TPD, Py-IR, and TG analyses and evaluated in the methanol to gasoline(MTG) reaction. Characterization results show that the hierarchical ZSM-5 aggregates possessed two kinds of mesopores, namely inter-and intra-crystalline mesopores. The amount of APAM considerably influenced the mesoporosity and textural properties of AHN-ZSM-5 zeolites. With the addition of APAM in the synthesis, the AHN-ZSM-5 zeolites exhibited large mesopore volume, large external surface area, and appropriate acidity. When applied in the MTG reaction, AHN-ZSM-5 demonstrated a catalytic lifetime that was 1.6 times longer than that of conventional ZSM-5 synthesized in the absence of APAM.展开更多
Intervertebral disc degeneration(IVDD)represents one of the most prevalent degenerative disorders,significantly impairing quality of life and overall health.The inflammatory cascade and dysregulated degradation of the...Intervertebral disc degeneration(IVDD)represents one of the most prevalent degenerative disorders,significantly impairing quality of life and overall health.The inflammatory cascade and dysregulated degradation of the extracellular matrix(ECM)triggered by pyroptosis are considered key mechanisms underlying the pathogenesis of IVDD.Research has highlighted the potential of Chuanxiong Rhizoma(CX),a traditional Chinese medicine,in exerting anti-pyroptotic effects;nevertheless,the exact mechanisms and pathways through which it modulates inflammation,as well as its potential role in the context of IVDD,remain unknown.Network pharmacology analysis was initially employed to identify the principal components and targets of Chuanxiong Rhizoma(CX)in the treatment of IVDD,ultimately selecting LIG as the key active ingredient responsible for its therapeutical effect.To explore the therapeutic effects of LIG on IVDD,we established a rat acupuncture model to simulate IVDD in vivo.Additionally,we used lipopolysaccharide(LPS)and adenosine triphosphate(ATP)to induce NPC degeneration model in vitro.In vitro experiments revealed that LIG treatment promotes the expression of anabolic markers such as CollagenⅡand Aggrecan while inhibiting the expression of catabolic markers MMP9 and MMP13 in nucleus pulposus cells(NPCs).Furthermore,LIG was also shown to inhibit cell pyroptosis and the secretion of inflammatory cytokines,which was mediated by NLRP3/caspase-1/GSDMD-N activation.Molecular docking and co-immunoprecipitation further demonstrated that LIG acts as a ligand for the Atg5 protein,inhibiting its degradation and promoting its interaction with the NLRP3 protein,ultimately leading to the suppression of pyroptosis activation in NPCs.It was also found that knocking down Atg5 reverses the protective effects of LIG and exacerbates pyroptosis-mediated inflammation in IVDD.In addition,in vivo experiments also showed that LIG delayed acupuncture-mediated IVDD development.Therefore,this paper confirmed that LIG has the ability to protect NPCs against pyroptosis through Atg5/NLRP3 axis and exert its therapeutic application to ameliorate disc degeneration in vivo.This may shed new insights into the role of LIG in IVDD treatment and offer a mechanistic basis for targeting NLRP3-mediated pyroptosis.展开更多
基金supported by the National Natural Science Foundation of China (No. 21276183)
文摘ZSM-5 aggregates consisting of superfine and hierarchical nanocrystals(combined with micropores and intra-crystalline mesopores) with an average size of 30 nm were prepared through one-pot synthesis with the assistance of anionic polyacrylamide(APAM). The resultant zeolites(AHN-ZSM-5) were characterized by XRD, ICP-OES, SEM, TEM, BET, NH_3-TPD, Py-IR, and TG analyses and evaluated in the methanol to gasoline(MTG) reaction. Characterization results show that the hierarchical ZSM-5 aggregates possessed two kinds of mesopores, namely inter-and intra-crystalline mesopores. The amount of APAM considerably influenced the mesoporosity and textural properties of AHN-ZSM-5 zeolites. With the addition of APAM in the synthesis, the AHN-ZSM-5 zeolites exhibited large mesopore volume, large external surface area, and appropriate acidity. When applied in the MTG reaction, AHN-ZSM-5 demonstrated a catalytic lifetime that was 1.6 times longer than that of conventional ZSM-5 synthesized in the absence of APAM.
基金supported by the National Natural Science Foundation of China(81972104,82272547)the Postdoctoral Scienceof China(2024M752336)+3 种基金the Natural Science Foundation of Jiangsu Province(BK20220252,BK20241795)Suzhou Key Clinical Disease Diagnosis and Treatment Technology Special Project(LCZX202203)Zhejiang Province Traditional Chinese Medicine Science and Technology Planning Project(2023ZR062)Clinical Research Project of the First Affiliated Hospital of Soochow University(BXLC017).
文摘Intervertebral disc degeneration(IVDD)represents one of the most prevalent degenerative disorders,significantly impairing quality of life and overall health.The inflammatory cascade and dysregulated degradation of the extracellular matrix(ECM)triggered by pyroptosis are considered key mechanisms underlying the pathogenesis of IVDD.Research has highlighted the potential of Chuanxiong Rhizoma(CX),a traditional Chinese medicine,in exerting anti-pyroptotic effects;nevertheless,the exact mechanisms and pathways through which it modulates inflammation,as well as its potential role in the context of IVDD,remain unknown.Network pharmacology analysis was initially employed to identify the principal components and targets of Chuanxiong Rhizoma(CX)in the treatment of IVDD,ultimately selecting LIG as the key active ingredient responsible for its therapeutical effect.To explore the therapeutic effects of LIG on IVDD,we established a rat acupuncture model to simulate IVDD in vivo.Additionally,we used lipopolysaccharide(LPS)and adenosine triphosphate(ATP)to induce NPC degeneration model in vitro.In vitro experiments revealed that LIG treatment promotes the expression of anabolic markers such as CollagenⅡand Aggrecan while inhibiting the expression of catabolic markers MMP9 and MMP13 in nucleus pulposus cells(NPCs).Furthermore,LIG was also shown to inhibit cell pyroptosis and the secretion of inflammatory cytokines,which was mediated by NLRP3/caspase-1/GSDMD-N activation.Molecular docking and co-immunoprecipitation further demonstrated that LIG acts as a ligand for the Atg5 protein,inhibiting its degradation and promoting its interaction with the NLRP3 protein,ultimately leading to the suppression of pyroptosis activation in NPCs.It was also found that knocking down Atg5 reverses the protective effects of LIG and exacerbates pyroptosis-mediated inflammation in IVDD.In addition,in vivo experiments also showed that LIG delayed acupuncture-mediated IVDD development.Therefore,this paper confirmed that LIG has the ability to protect NPCs against pyroptosis through Atg5/NLRP3 axis and exert its therapeutic application to ameliorate disc degeneration in vivo.This may shed new insights into the role of LIG in IVDD treatment and offer a mechanistic basis for targeting NLRP3-mediated pyroptosis.
