Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.Howeve...Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.However, the presence of structural isomers varying in C=C double bond(DB) locations makes isomerresolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics.展开更多
Stomachache is the main symptom of stomach duct pain near the heart.Professor Xiaoquan Du is experienced in treating spleen and stomach diseases with traditional Chinese medicine(TCM)syndrome differentiation.He has va...Stomachache is the main symptom of stomach duct pain near the heart.Professor Xiaoquan Du is experienced in treating spleen and stomach diseases with traditional Chinese medicine(TCM)syndrome differentiation.He has vast practical TCM experience through years of clinical diagnosis and treatment,and incorporated his own characteristic methods in treatments,thereby developing seven methods for the treatment of stomachache.Clinically,methods such as“invigorating the spleen and benefiting the stomach”and“wanning the middle and dispersing cold”are mostly adopted to treat stomach duct pain.展开更多
A systematic collection of relevant literature on acupuncture and moxibustion treatment of stomach pain,and a summary of the rule of acupoints selection for acupuncture treatment of stomach pain.In conclusion,Zusanli,...A systematic collection of relevant literature on acupuncture and moxibustion treatment of stomach pain,and a summary of the rule of acupoints selection for acupuncture treatment of stomach pain.In conclusion,Zusanli,Zhongwan,Neiguan,and Liang Qiu are selected as the main acupoints.At the same time,according to the different syndrome types,dyspepsia hurt a stomach syndrome is compatible with Burong,Chengman,Tianshu and Shangjuxu acupoints.Cold evil invading the stomach syndrome is compatible with Shenque and Qihai acupoints.Liver-Qi attacking stomach syndrome is compatible with Danzhong,Zhangmen,and Qimen acupoints;for blood stasis to stop the stomach,it is compatible with Xuehai,Geshu,and Pishu acupoints.Adopting this rule of acupoints selection has a significant effect on acupuncture treatment of stomach pain,and it also provides a reference for other doctors to treat stomach pain.展开更多
Acid regurgitation is a common symptom in digestive system diseases which is mainly caused by emotions,diet,and other factors.It is related to gastroesophageal reflux disease,chronic gastritis,and other diseases in We...Acid regurgitation is a common symptom in digestive system diseases which is mainly caused by emotions,diet,and other factors.It is related to gastroesophageal reflux disease,chronic gastritis,and other diseases in Western medicine.According to modem medical research,acid reflux is related to Helicobacter pylori(HP)infection,weak effect of anti-reflux barrier,decreased clearance of esophageal acid,dysfunction of esophageal mucosal barrier,strong stimulation of reflux on esophageal mucosal wall,and other factors[1].It can be divided into four types which are heat,cold,food stagnation,and yin deficiency It is often accompanied by vomiting,heartbum,stomachache,and other symptoms.Clinically,it is a mixture of cold and heat,as well as excess and deficiency.Professor Du's self-made method of“first soothing,second reducing,and third harmonizmg”(soothing liver and stomach,relieving stomach qi,and neutralizing stomach acid)is used to treat both,symptoms and the root causes.The commonly used prescriptions are left-running metal pill,cuttlefish bone and fritillaria powder,calcined ark shell,oyster shell,etc.展开更多
Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys...Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19.展开更多
Small biomolecules(m/z<500)are the material basis of organisms and participate in life activities,but their comprehensive and accurate detection in complex samples remains a challenge.Matrix-assisted laser desorpti...Small biomolecules(m/z<500)are the material basis of organisms and participate in life activities,but their comprehensive and accurate detection in complex samples remains a challenge.Matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is a powerful detection tool for molecular analysis with high throughput.The development of a new matrix is essential to improve the efficiency of the MALDI-MS for molecular compound detection.In this work,the sandwich-like gold nanoparticles@mesoporous silica nanocomposite@silver nanoparticles(Au@MSN@Ag)nanospheres were prepared by layer-by-layer super-assembly strategy,and can be used as a novel matrix for the quantitative detection and enrichment of small biomolecules by LDI-MS.The sandwich-like nanospheres form a unique plasma resonant cavity that effectively absorbs the laser energy,while the homogeneous mesoporous structure of MSN can lock the analyte,which is essential for efficient LDI of small molecules.Compared to traditional matrices,Au@MSN@Ag shows the advantages of low background,wide application range,high sensitivity,super high salt and protein tolerance,and good stability.For example,the detection limit of glucose was as low as 5 fmol,and showed a good linear relationship in the range of 1−750μg/mL.Au@MSN@Ag assisted LDI-MS allows the enrichment and detection of small molecules in traditional Chinese medicine(TCM)without derivatization and purification,classification of herbs using the accurate quantitative results oligosaccharides,and identification of gelatin by amino acid content.This research could help in designing more efficient nanostructure matrices and further explored the application of LDI-MS.展开更多
Neurological manifestations are frequently reported in the COVID-19 patients.Neuromechanism of SARS-CoV-2 remains to be elucidated.In this study,we explored the mechanisms of SARS-CoV-2 neurotropism via our establishe...Neurological manifestations are frequently reported in the COVID-19 patients.Neuromechanism of SARS-CoV-2 remains to be elucidated.In this study,we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19.In rhesus monkey,SARS-CoV-2 invades the CNS primarily via the olfactory bulb.Thereafter,viruses rapidly spread to functional areas of the central nervous system,such as hippocampus,thalamus,and medulla oblongata.The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons,microglia,and astrocytes in the CNS.Consistently,SARS-CoV-2 infects neuro-derived SK-N-SH,glial-derived U251,and brain microvascular endothelial cells in vitro.To our knowledge,this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model,which provides important insights into the CNS-related pathogenesis of SARS-CoV-2.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 81903571, 21904080)the Taishan Scholars the Taishan Scholars Program (C. Sun, No. tsqn202103096)the Shandong Province “Double-Hundred Talent Plan” Program。
文摘Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.However, the presence of structural isomers varying in C=C double bond(DB) locations makes isomerresolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics.
文摘Stomachache is the main symptom of stomach duct pain near the heart.Professor Xiaoquan Du is experienced in treating spleen and stomach diseases with traditional Chinese medicine(TCM)syndrome differentiation.He has vast practical TCM experience through years of clinical diagnosis and treatment,and incorporated his own characteristic methods in treatments,thereby developing seven methods for the treatment of stomachache.Clinically,methods such as“invigorating the spleen and benefiting the stomach”and“wanning the middle and dispersing cold”are mostly adopted to treat stomach duct pain.
文摘A systematic collection of relevant literature on acupuncture and moxibustion treatment of stomach pain,and a summary of the rule of acupoints selection for acupuncture treatment of stomach pain.In conclusion,Zusanli,Zhongwan,Neiguan,and Liang Qiu are selected as the main acupoints.At the same time,according to the different syndrome types,dyspepsia hurt a stomach syndrome is compatible with Burong,Chengman,Tianshu and Shangjuxu acupoints.Cold evil invading the stomach syndrome is compatible with Shenque and Qihai acupoints.Liver-Qi attacking stomach syndrome is compatible with Danzhong,Zhangmen,and Qimen acupoints;for blood stasis to stop the stomach,it is compatible with Xuehai,Geshu,and Pishu acupoints.Adopting this rule of acupoints selection has a significant effect on acupuncture treatment of stomach pain,and it also provides a reference for other doctors to treat stomach pain.
文摘Acid regurgitation is a common symptom in digestive system diseases which is mainly caused by emotions,diet,and other factors.It is related to gastroesophageal reflux disease,chronic gastritis,and other diseases in Western medicine.According to modem medical research,acid reflux is related to Helicobacter pylori(HP)infection,weak effect of anti-reflux barrier,decreased clearance of esophageal acid,dysfunction of esophageal mucosal barrier,strong stimulation of reflux on esophageal mucosal wall,and other factors[1].It can be divided into four types which are heat,cold,food stagnation,and yin deficiency It is often accompanied by vomiting,heartbum,stomachache,and other symptoms.Clinically,it is a mixture of cold and heat,as well as excess and deficiency.Professor Du's self-made method of“first soothing,second reducing,and third harmonizmg”(soothing liver and stomach,relieving stomach qi,and neutralizing stomach acid)is used to treat both,symptoms and the root causes.The commonly used prescriptions are left-running metal pill,cuttlefish bone and fritillaria powder,calcined ark shell,oyster shell,etc.
基金supported by the National Research and Development Project of China(2020YFC0841100,2020YFC0846400,and 2020YFA0707600)CAMS Innovation Fund for Medical Sciences(2016-I2M-2-006 and 2020-I2M-CoV19-012)+1 种基金the Talents Project of Yunnan Province(2017HB068)the Special Funds for High-level Health and Family Planning Technical Personnel training of Yunnan Province(D-201653).
文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19.
基金the National Key R&D Program of China(Nos.2019YFC1604600,2017YFA0206901,2019YFC1604601,2017YFA0206900,and 2018YFC1602301)the National Natural Science Foundation of China(Nos.21705027,2197402951808328,61903235,42007218,and 51703109)+5 种基金the Major Scientific and Technological Innovation Project of Shandong(Nos.2018CXGC1406,2019JZZY010457 and 2019JZZY020309)The Natural Science Foundation of Shanghai(18ZR1404700)Construction Project of Shanghai Key Laboratory of Molecular Imaging(18DZ2260400)Shanghai Municipal Education Commission(Class II Plateau Disciplinary Construction Program of Medical Technology of SUMHS,2018-2020)The ability establishment of sustainable use for valuable Chinese medicine resources(2060302)The natural Science Foundation of Shandong Province,China(ZR2020QE228).
文摘Small biomolecules(m/z<500)are the material basis of organisms and participate in life activities,but their comprehensive and accurate detection in complex samples remains a challenge.Matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is a powerful detection tool for molecular analysis with high throughput.The development of a new matrix is essential to improve the efficiency of the MALDI-MS for molecular compound detection.In this work,the sandwich-like gold nanoparticles@mesoporous silica nanocomposite@silver nanoparticles(Au@MSN@Ag)nanospheres were prepared by layer-by-layer super-assembly strategy,and can be used as a novel matrix for the quantitative detection and enrichment of small biomolecules by LDI-MS.The sandwich-like nanospheres form a unique plasma resonant cavity that effectively absorbs the laser energy,while the homogeneous mesoporous structure of MSN can lock the analyte,which is essential for efficient LDI of small molecules.Compared to traditional matrices,Au@MSN@Ag shows the advantages of low background,wide application range,high sensitivity,super high salt and protein tolerance,and good stability.For example,the detection limit of glucose was as low as 5 fmol,and showed a good linear relationship in the range of 1−750μg/mL.Au@MSN@Ag assisted LDI-MS allows the enrichment and detection of small molecules in traditional Chinese medicine(TCM)without derivatization and purification,classification of herbs using the accurate quantitative results oligosaccharides,and identification of gelatin by amino acid content.This research could help in designing more efficient nanostructure matrices and further explored the application of LDI-MS.
基金This study was supported by the National Key R&D Project of China(2020YFA0707602,2020YFC0846400,2020YFC0841100)CAMS Innovation Fund for Medical Sciences(2016-I2M-2-001,2016-I2M-2-006,and 2020-I2M-COV19-012)+1 种基金Yunnan Key R&D Project(202003AC100003)The Talents Project of Yunnan Province(2017HB068).
文摘Neurological manifestations are frequently reported in the COVID-19 patients.Neuromechanism of SARS-CoV-2 remains to be elucidated.In this study,we explored the mechanisms of SARS-CoV-2 neurotropism via our established non-human primate model of COVID-19.In rhesus monkey,SARS-CoV-2 invades the CNS primarily via the olfactory bulb.Thereafter,viruses rapidly spread to functional areas of the central nervous system,such as hippocampus,thalamus,and medulla oblongata.The infection of SARS-CoV-2 induces the inflammation possibly by targeting neurons,microglia,and astrocytes in the CNS.Consistently,SARS-CoV-2 infects neuro-derived SK-N-SH,glial-derived U251,and brain microvascular endothelial cells in vitro.To our knowledge,this is the first experimental evidence of SARS-CoV-2 neuroinvasion in the NHP model,which provides important insights into the CNS-related pathogenesis of SARS-CoV-2.
