The exchange of information and materials between organelles plays a crucial role in regulating cellular physiological functions and metabolic levels.Mitochondria-associated endoplasmic reticulum membranes serve as ph...The exchange of information and materials between organelles plays a crucial role in regulating cellular physiological functions and metabolic levels.Mitochondria-associated endoplasmic reticulum membranes serve as physical contact channels between the endoplasmic reticulum membrane and the mitochondrial outer membrane,formed by various proteins and protein complexes.This microstructural domain mediates several specialized functions,including calcium(Ca^(2+))signaling,autophagy,mitochondrial morphology,oxidative stress response,and apoptosis.Notably,the dysregulation of Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes is a critical factor in the pathogenesis of neurological diseases.Certain proteins or protein complexes within these membranes directly or indirectly regulate the distance between the endoplasmic reticulum and mitochondria,as well as the transduction of Ca^(2+)signaling.Conversely,Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes influences other mitochondria-associated endoplasmic reticulum membraneassociated functions.These functions can vary significantly across different neurological diseases—such as ischemic stroke,traumatic brain injury,Alzheimer's disease,Parkinson's disease,amyotrophic lateral sclerosis,and Huntington's disease—and their respective stages of progression.Targeted modulation of these disease-related pathways and functional proteins can enhance neurological function and promote the regeneration and repair of damaged neurons.Therefore,mitochondria-associated endoplasmic reticulum membranes-mediated Ca^(2+)signaling plays a pivotal role in the pathological progression of neurological diseases and represents a significant potential therapeutic target.This review focuses on the effects of protein complexes in mitochondria-associated endoplasmic reticulum membranes and the distinct roles of mitochondria-associated endoplasmic reticulum membranes-mediated Ca^(2+)signaling in neurological diseases,specifically highlighting the early protective effects and neuronal damage that can result from prolonged mitochondrial Ca^(2+)overload or deficiency.This article provides a comprehensive analysis of the various mechanisms of Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes in neurological diseases,contributing to the exploration of potential therapeutic targets for promoting neuroprotection and nerve repair.展开更多
Based on a comprehensive eco-geochemical survey of Shandong Province,the differences in pesticide residue situation and contents of DDTs and HCHs in the soils of Yantai City and Southwest Shandong Province(abbreviated...Based on a comprehensive eco-geochemical survey of Shandong Province,the differences in pesticide residue situation and contents of DDTs and HCHs in the soils of Yantai City and Southwest Shandong Province(abbreviated as SSP)were studied in this paper.The results showed that the detection rates of DDTs and HCHs in the soil of SSP were apparently higher than Yantai City.However,the mean contents of DDTs and HCHs in the soil of Yantai were 9 and 25 times of SSP,respectively,and higher than the mean contents of many other cities and areas.p,p′-DDE was the main pesticide residual form in the soil of SSP.It is recognized as the degradation product of DDTs in oxidation environment in past,nevertheless,5.52%of the soils in Yantai City still have a little of DDT input recently,which has posed a big impact on soil environment quality.β-HCH was the main form of the four isomers of HCHs in Yantai City,andα-HCH/γ-HCH in SSP was low.These reveal that the residual time of HCHs in the soils of both the two areas has a long period.After primary analysis,the risk of DDTs and HCHs in the soil of SSP is low,but DDTs in the soil of Yantai City still has a certain risk.展开更多
Objective:To provide new insights for the evaluation and diagnosis of chronic neck pain(CNP).Methods:22 patients with CNP and 22 healthy individuals were recruited from South China Normal University,who were all colle...Objective:To provide new insights for the evaluation and diagnosis of chronic neck pain(CNP).Methods:22 patients with CNP and 22 healthy individuals were recruited from South China Normal University,who were all college students.The subjects'neck extensor muscle strength in the head neutral position,the natural anteversion position,and the maximum forward flexion position were measured by an isometric muscle strength tester respectively.The neck extensor strength of CNP patients and healthy subjects were compared.Results:In the neutral position,the maximum isometric muscle strength of neck extensor muscles was 12.31 kg for CNP patients and 15.16 kg for healthy individuals,resulting in a ratio of 81%strength in patients compared to healthy subjects.This difference was highly significant(P<0.000).In the natural anteversion position,the respective values were 12.6 kg for CNP patients and 15.05 kg for healthy individuals,with a ratio of 83%,and a significant difference between groups(P<0.001).In the maximum forward flexion position of the head,the values were 13.36 kg for CNP patients and 16.15 kg for healthy individuals,with a ratio of 82%,and a highly significant difference(P<0.000).Conclusion:The neck extensor muscle strength levels in college students with CNP were significantly lower compared to healthy college students across all measured positions.展开更多
Traditional fluorescent emitters cannot eff ectively utilize triplet excitons owing to the spin statistical limitation, thus their electrochemiluminescence(ECL) efficiency(Φ_(ECL)) is relatively low. Improving the ut...Traditional fluorescent emitters cannot eff ectively utilize triplet excitons owing to the spin statistical limitation, thus their electrochemiluminescence(ECL) efficiency(Φ_(ECL)) is relatively low. Improving the utilization efficiency of triplet excitons is of great significance for developing efficient luminescent materials. Here we designed a hot exciton molecule(NZ2TPA) containing highly efficient chromophore naphthothiadiazole as an electron acceptor and triphenylamine(TPA) with aggregation induced emission(AIE) property as the strong electron donor to synthesize an ECL nanoemitter-NZ2TPA nanoparticles(NT NPs). The hybridized local and charge-transfer(HLCT) excited state of NZ2TPA achieved a high exciton utilization through the reverse intersystem crossing from higher triplet states( h RISC). The combination of HLCT and AIE characteristics endowed NT NPs with superior ΦECLover other nanoemitters, which provided an excellent material for the design of highly sensitive ECL biosensors. Using alkaline phosphatase(ALP) as an analyte model, a “signal-on” ECL biosensing approach was constructed by combining the quenching of manganese dioxide nanosheets(MnO_(2)NSs) on ECL emission of NT NPs and the reduction of MnO_(2)by ascorbic acid produced from ALP-catalyzed dephosphorylation, which showed a detectable range of 0.004-400 U/L with a detection limit of 0.57 mU/L. The excellent performance demonstrated the immense potential of organic nanomaterials through combining HLCT and AIE properties to improve ΦECL.展开更多
基金supported by Yunnan Province Innovation Team of Prevention and Treatment for Brain Disease with Acupuncture and Tuina,No.202405AS350007Youth Top Talent Project of 10-thousand Talent Plan in Yunnan Province,No.YNWR-QNBJ-2018-345+3 种基金the National Natural Science Foundation of China,No.81960731Joint Special Project of Traditional Chinese Medicine in Science and Technology Department of Yunnan Province,Nos.2019FF002[-008],202001AZ070001-002 and 202001AZ070001-030Yunnan Province University Innovation Team Projects No.2019YGC04Yunnan Province Project Education Fund,Nos.2024Y406,2024Y414(all to PZ)。
文摘The exchange of information and materials between organelles plays a crucial role in regulating cellular physiological functions and metabolic levels.Mitochondria-associated endoplasmic reticulum membranes serve as physical contact channels between the endoplasmic reticulum membrane and the mitochondrial outer membrane,formed by various proteins and protein complexes.This microstructural domain mediates several specialized functions,including calcium(Ca^(2+))signaling,autophagy,mitochondrial morphology,oxidative stress response,and apoptosis.Notably,the dysregulation of Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes is a critical factor in the pathogenesis of neurological diseases.Certain proteins or protein complexes within these membranes directly or indirectly regulate the distance between the endoplasmic reticulum and mitochondria,as well as the transduction of Ca^(2+)signaling.Conversely,Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes influences other mitochondria-associated endoplasmic reticulum membraneassociated functions.These functions can vary significantly across different neurological diseases—such as ischemic stroke,traumatic brain injury,Alzheimer's disease,Parkinson's disease,amyotrophic lateral sclerosis,and Huntington's disease—and their respective stages of progression.Targeted modulation of these disease-related pathways and functional proteins can enhance neurological function and promote the regeneration and repair of damaged neurons.Therefore,mitochondria-associated endoplasmic reticulum membranes-mediated Ca^(2+)signaling plays a pivotal role in the pathological progression of neurological diseases and represents a significant potential therapeutic target.This review focuses on the effects of protein complexes in mitochondria-associated endoplasmic reticulum membranes and the distinct roles of mitochondria-associated endoplasmic reticulum membranes-mediated Ca^(2+)signaling in neurological diseases,specifically highlighting the early protective effects and neuronal damage that can result from prolonged mitochondrial Ca^(2+)overload or deficiency.This article provides a comprehensive analysis of the various mechanisms of Ca^(2+)signaling mediated by mitochondria-associated endoplasmic reticulum membranes in neurological diseases,contributing to the exploration of potential therapeutic targets for promoting neuroprotection and nerve repair.
基金Supported by Shandong Province Land and Resources Survey Project(2006709)Ministry and Province Cooperation Project(1212010310306)
文摘Based on a comprehensive eco-geochemical survey of Shandong Province,the differences in pesticide residue situation and contents of DDTs and HCHs in the soils of Yantai City and Southwest Shandong Province(abbreviated as SSP)were studied in this paper.The results showed that the detection rates of DDTs and HCHs in the soil of SSP were apparently higher than Yantai City.However,the mean contents of DDTs and HCHs in the soil of Yantai were 9 and 25 times of SSP,respectively,and higher than the mean contents of many other cities and areas.p,p′-DDE was the main pesticide residual form in the soil of SSP.It is recognized as the degradation product of DDTs in oxidation environment in past,nevertheless,5.52%of the soils in Yantai City still have a little of DDT input recently,which has posed a big impact on soil environment quality.β-HCH was the main form of the four isomers of HCHs in Yantai City,andα-HCH/γ-HCH in SSP was low.These reveal that the residual time of HCHs in the soils of both the two areas has a long period.After primary analysis,the risk of DDTs and HCHs in the soil of SSP is low,but DDTs in the soil of Yantai City still has a certain risk.
文摘Objective:To provide new insights for the evaluation and diagnosis of chronic neck pain(CNP).Methods:22 patients with CNP and 22 healthy individuals were recruited from South China Normal University,who were all college students.The subjects'neck extensor muscle strength in the head neutral position,the natural anteversion position,and the maximum forward flexion position were measured by an isometric muscle strength tester respectively.The neck extensor strength of CNP patients and healthy subjects were compared.Results:In the neutral position,the maximum isometric muscle strength of neck extensor muscles was 12.31 kg for CNP patients and 15.16 kg for healthy individuals,resulting in a ratio of 81%strength in patients compared to healthy subjects.This difference was highly significant(P<0.000).In the natural anteversion position,the respective values were 12.6 kg for CNP patients and 15.05 kg for healthy individuals,with a ratio of 83%,and a significant difference between groups(P<0.001).In the maximum forward flexion position of the head,the values were 13.36 kg for CNP patients and 16.15 kg for healthy individuals,with a ratio of 82%,and a highly significant difference(P<0.000).Conclusion:The neck extensor muscle strength levels in college students with CNP were significantly lower compared to healthy college students across all measured positions.
基金funded by by National Natural Science Foundation of China (21890741,21827812)。
文摘Traditional fluorescent emitters cannot eff ectively utilize triplet excitons owing to the spin statistical limitation, thus their electrochemiluminescence(ECL) efficiency(Φ_(ECL)) is relatively low. Improving the utilization efficiency of triplet excitons is of great significance for developing efficient luminescent materials. Here we designed a hot exciton molecule(NZ2TPA) containing highly efficient chromophore naphthothiadiazole as an electron acceptor and triphenylamine(TPA) with aggregation induced emission(AIE) property as the strong electron donor to synthesize an ECL nanoemitter-NZ2TPA nanoparticles(NT NPs). The hybridized local and charge-transfer(HLCT) excited state of NZ2TPA achieved a high exciton utilization through the reverse intersystem crossing from higher triplet states( h RISC). The combination of HLCT and AIE characteristics endowed NT NPs with superior ΦECLover other nanoemitters, which provided an excellent material for the design of highly sensitive ECL biosensors. Using alkaline phosphatase(ALP) as an analyte model, a “signal-on” ECL biosensing approach was constructed by combining the quenching of manganese dioxide nanosheets(MnO_(2)NSs) on ECL emission of NT NPs and the reduction of MnO_(2)by ascorbic acid produced from ALP-catalyzed dephosphorylation, which showed a detectable range of 0.004-400 U/L with a detection limit of 0.57 mU/L. The excellent performance demonstrated the immense potential of organic nanomaterials through combining HLCT and AIE properties to improve ΦECL.
