A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and...A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and laccase-like)and electrochemical activity.CuO@3-APBA nanoparticles(CuO@3-APBA NPs)were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid(3-APBA)in CuO NPs.3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions.Then,a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme.For the first signal,CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene(TMB)to turn the solution from colorless to blue in the presence of H_(2)O_(2)(absorbance at 652 nm).For the second signal,CuO@3-APBA can catalyze the oxidation of substrates(2,4-dichlorophenol and 4-aminoantipyrine)and turn the solution from colorless to pink(absorbance at 510 nm).For the third signal,electrochemical oxidation peak of copper ion from Cu^(+)to Cu^(2+)of Cu O@3-APBA was recorded by differential pulse voltammetry,which was used to determine the OM.The sensing platform exhibited a wide linear range(0.0000316-100.000000 ng/mL)with a low detection limit(0.0105 pg/mL),as well as showed advantages,such as satisfactory reproducibility,good stability,and excellent selectivity.The assay has the potential applications for ultrasensitive detection of allergen in foods.展开更多
Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electroche...Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.展开更多
Rational fertilization is an important measure to increase crop yield and soil fertility. Through analysis,this paper aims to master the change characteristics of soil organic matter and rice yield under different fer...Rational fertilization is an important measure to increase crop yield and soil fertility. Through analysis,this paper aims to master the change characteristics of soil organic matter and rice yield under different fertilizer treatments,in order to provide an important reference for the sustainable use of soil and effective fertilization. Long-term( 19 years) rice crop rotation experiments in waterloggogenic paddy soil were conducted to investigate the change trend of crop grain yield and soil organic matter with time,reveal the dynamic characteristics and relationship between main fertility factors and crop yields using comparative analysis at three sites with conventional fertilization and non-fertilization in Guilin. The results showed that compared with previous years,the rice yield increased by 53% under the fertilization treatment and degreased by 66% under the control. Over the years,the average soil organic matter( SOM) content under fertilization treatment was 23% higher than under CK treatment. This indicates that chemical fertilizer and organic manure application can increase the rice yield and soil organic matter,and high rice yield can be attributed to the SOM increase.展开更多
In this experiment,two-factor 7-level uniform design scheme was applied.Separate treatment of each factor was performed for single effect test.Then,it analyzed the effects of acid and aluminum stress under soilless cu...In this experiment,two-factor 7-level uniform design scheme was applied.Separate treatment of each factor was performed for single effect test.Then,it analyzed the effects of acid and aluminum stress under soilless culture on the water content of plant during the growth of Ginkgo biloba L.seedlings,so as to provide a theoretical basis for studying the connection between plant water and physiological stress.The results showed that the water content of G.biloba plants declined with the decrease of p H,but the water content of G.biloba plants declined with the increase in the concentration of aluminum treatment,indicating that the stronger the acid and aluminum stress,the lower the water content of the plants,affecting the normal absorption of water content of G.biloba plants,accordingly leading to lack of water.According to the analysis of the degree of influence on the water content,p H is the first factor influencing the growth of G.biloba plants.If p H is lower than 3.5,G.biloba plants will not growth,while p H is higher than 5.0,aluminum has no significant effect on the water content of G.biloba plants.In conclusion,the water content of G.biloba plants can be used as an essential indicator for acid and aluminum stress.展开更多
基金financially supported by Major Science and Technology Project of Yunnan Province(202302AE090022)the National Natural Science Foundation of China(21764005,32160236)+4 种基金National Key Research and Development Program of China(2022YFC2601604)Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)the Second Phase of“Double-First Class”Program Construction of Yunnan UniversityProgram for Donglu Scholars of Yunnan Universitythe Research Innovation Fund for Graduate Students of School of Chemical Science and Technology,Yunnan University。
文摘A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and laccase-like)and electrochemical activity.CuO@3-APBA nanoparticles(CuO@3-APBA NPs)were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid(3-APBA)in CuO NPs.3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions.Then,a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme.For the first signal,CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene(TMB)to turn the solution from colorless to blue in the presence of H_(2)O_(2)(absorbance at 652 nm).For the second signal,CuO@3-APBA can catalyze the oxidation of substrates(2,4-dichlorophenol and 4-aminoantipyrine)and turn the solution from colorless to pink(absorbance at 510 nm).For the third signal,electrochemical oxidation peak of copper ion from Cu^(+)to Cu^(2+)of Cu O@3-APBA was recorded by differential pulse voltammetry,which was used to determine the OM.The sensing platform exhibited a wide linear range(0.0000316-100.000000 ng/mL)with a low detection limit(0.0105 pg/mL),as well as showed advantages,such as satisfactory reproducibility,good stability,and excellent selectivity.The assay has the potential applications for ultrasensitive detection of allergen in foods.
基金financially supported by National Key Research and Development Program of China(2022YFC2601604)Major science and technology project of Yunnan Province(202202AE090085)+9 种基金the National Natural Science Foundation of China(3216059732160236)Science and technology talent and platform plan of YunnanKey Scientific and Technology Project of Yunnan(202203AC100010)Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)the second phase of“Double-First Class”program construction of Yunnan Universitygrants from State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University(2021KF005)Key Scientific and Technology Project of Yunnan(202002AE320005)Program for Excellent Young Talents of Yunnan Universitythe Program for Donglu Scholars of Yunnan University。
文摘Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.
基金Supported by National Natural Science Foundation of China(41361068)Key Science and Technology Research Funds for Guangxi Universities(2013ZD067)Guangxi Natural Science Foundation(2011GXNSFB-018056)
文摘Rational fertilization is an important measure to increase crop yield and soil fertility. Through analysis,this paper aims to master the change characteristics of soil organic matter and rice yield under different fertilizer treatments,in order to provide an important reference for the sustainable use of soil and effective fertilization. Long-term( 19 years) rice crop rotation experiments in waterloggogenic paddy soil were conducted to investigate the change trend of crop grain yield and soil organic matter with time,reveal the dynamic characteristics and relationship between main fertility factors and crop yields using comparative analysis at three sites with conventional fertilization and non-fertilization in Guilin. The results showed that compared with previous years,the rice yield increased by 53% under the fertilization treatment and degreased by 66% under the control. Over the years,the average soil organic matter( SOM) content under fertilization treatment was 23% higher than under CK treatment. This indicates that chemical fertilizer and organic manure application can increase the rice yield and soil organic matter,and high rice yield can be attributed to the SOM increase.
基金Supported by Project of National Natural Science Foundation(41361068)Natural Science Foundation Project of Guangxi(2016GXNSFAA380183,2016GXNSFAA380160)Professor Startup Fund of Hezhou University(HZUJS201611)
文摘In this experiment,two-factor 7-level uniform design scheme was applied.Separate treatment of each factor was performed for single effect test.Then,it analyzed the effects of acid and aluminum stress under soilless culture on the water content of plant during the growth of Ginkgo biloba L.seedlings,so as to provide a theoretical basis for studying the connection between plant water and physiological stress.The results showed that the water content of G.biloba plants declined with the decrease of p H,but the water content of G.biloba plants declined with the increase in the concentration of aluminum treatment,indicating that the stronger the acid and aluminum stress,the lower the water content of the plants,affecting the normal absorption of water content of G.biloba plants,accordingly leading to lack of water.According to the analysis of the degree of influence on the water content,p H is the first factor influencing the growth of G.biloba plants.If p H is lower than 3.5,G.biloba plants will not growth,while p H is higher than 5.0,aluminum has no significant effect on the water content of G.biloba plants.In conclusion,the water content of G.biloba plants can be used as an essential indicator for acid and aluminum stress.
